Abstract:
BACKGROUND:We tested the hypothesis that combined cyclooxygenase-1 (COX-1) and prostacyclin synthase (PGIS) gene transfer selectively augments prostacyclin production without a concurrent overproduction of other prostanoids. METHODS AND RESULTS:ECV304 cells were transfected with bicistronic pCOX-1/PGIS versus pCOX-1 or pPGIS, and prostanoids were analyzed. Contrary to the high prostaglandin E2 synthesis in pCOX-1 transfected cells, selective prostacyclin formation was noted with bicistronic plasmid transfection. Next, we determined the optimal ratio of Ad-COX-1 to Ad-PGIS by transfecting human umbilical vein endothelial cells with various titers of these 2 adenoviral constructs and determined the level of protein expression and prostanoid synthesis. Our results show that optimal ratios of adenoviral titers to achieve a large prostacyclin augmentation without overproduction of prostaglandin E2 or F2alpha were 50 to 100 plaque forming units (pfu) of Ad-COX-1 to 50 pfu of Ad-PGIS per cell. A higher Ad-PGIS to Ad-COX-1 ratio caused a paradoxical decline in prostacyclin synthesis. CONCLUSIONS:Prostacyclin synthesis can be selectively augmented by cotransfecting endothelial cells with an optimal ratio of COX-1 to PGIS. Combined COX-1 and PGIS gene transfer has the potential for therapeutic augmentation of prostacyclin.
journal_name
Circulationjournal_title
Circulationauthors
Shyue SK,Tsai MJ,Liou JY,Willerson JT,Wu KKdoi
10.1161/01.cir.103.16.2090subject
Has Abstractpub_date
2001-04-24 00:00:00pages
2090-5issue
16eissn
0009-7322issn
1524-4539journal_volume
103pub_type
杂志文章相关文献
CIRCULATION文献大全abstract:BACKGROUND:A variable degree of smooth muscle cell (SMC) proliferation after balloon injury has been reported in previous rat studies. It is unknown whether balloon injury induces c-fos expression and whether it is related to the degree of vascular injury in vivo. Therefore, we tested the hypothesis that proportional i...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.92.5.1230
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Although autonomic imbalance is known to be characteristics of patients with clinically overt symptomatic congestive heart failure, it is currently unknown whether this autonomic response arises early in the course of left ventricular dysfunction or is restricted to the later stages of circulatory failure. ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.92.3.555
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND:Pollution by particulates has been consistently associated with increased cardiovascular morbidity and mortality. However, the mechanisms responsible for these effects are not well-elucidated. METHODS AND RESULTS:To assess to what extent and how rapidly inhaled pollutant particles pass into the systemic cir...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/hc0402.104118
更新日期:2002-01-29 00:00:00
abstract:BACKGROUND:Various measures of arterial stiffness and wave reflection have been proposed as cardiovascular risk markers. Prior studies have not assessed relations of a comprehensive panel of stiffness measures to prognosis in the community. METHODS AND RESULTS:We used proportional hazards models to analyze first-onset...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.109.886655
更新日期:2010-02-02 00:00:00
abstract:BACKGROUND:In our multicenter study of the total cavopulmonary connection (TCPC), a cohort of patients with long-segment left pulmonary artery (LPA) stenosis was observed (35%). The clinically recognized detrimental effects of LPA stenosis motivated a computational fluid dynamic simulation study within 3-dimensional pa...
journal_title:Circulation
pub_type: 杂志文章,多中心研究
doi:10.1161/CIRCULATIONAHA.104.530931
更新日期:2005-11-22 00:00:00
abstract:BACKGROUND:Endothelial nitric oxide (NO)-mediated responses are impaired in arterioles of male spontaneously hypertensive rats (SHR), but they are still present in female SHR. We hypothesized that in vitro incubation of arterioles of male SHR with estrogen will restore NO-mediated responses by upregulation of endotheli...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.101.1.94
更新日期:2000-01-04 00:00:00
abstract:BACKGROUND:Children awaiting heart transplantation have severe limitations in their ability to exercise. The purpose of this study was to assess exercise capacity after pediatric heart transplantation and to identify factors influencing exercise performance. METHODS AND RESULTS:Progressive cycle ergometry testing was ...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND:Although the use of physical signs for the diagnosis of ventricular tachycardia (VT) was described in the early 1900s, their value in this role has never been systematically assessed. METHODS AND RESULTS:Using a blinded, randomized protocol, we examined the ability of 26 clinicians to detect ventriculoatria...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.cir.90.6.3103
更新日期:1994-12-01 00:00:00
abstract::Real-time cross-sectional echocardiographic studies of the main coronary arteries were performed in 20 normal subjects, 12 patients with patent ductus arteriosus and 14 patients with coronary artery fistula in whom the diagnosis was established by angiography. In 12 patients, the coronary artery that formed the fistul...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.65.3.600
更新日期:1982-03-01 00:00:00
abstract:BACKGROUND:Treatment of aortocaval fistulae (ACFs) by open surgery is known to be a high-risk procedure. In this experimental study, we developed a percutaneous model of ACF to test a new nonsurgical method of treatment of ACF using endovascular stent grafts. METHODS AND RESULTS:An ACF was created percutaneously in ei...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.94.1.108
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Erosion and rupture of coronary atheromas are the events preceding the vast majority of acute coronary syndromes. The shoulder regions of atheromas, the sites at which erosion or rupture is most likely to occur, are the sites at which mast cells accumulate. These cells are filled with neutral proteases capab...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.92.5.1084
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Sodium glucose cotransporter 2 inhibitors may reduce kidney hyperfiltration, thereby preventing diabetic kidney disease progression, which may in turn reduce cardiovascular risk, including heart failure. However, the mechanisms that regulate renal function responses to sodium glucose cotransporter 2 inhibiti...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.118.037418
更新日期:2019-07-23 00:00:00
abstract:BACKGROUND:Despite a recent American Heart Association (AHA) consensus statement emphasizing the importance of resistant hypertension, the incidence and prognosis of this condition are largely unknown. METHODS AND RESULTS:This retrospective cohort study in 2 integrated health plans included patients with incident hype...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.111.068064
更新日期:2012-04-03 00:00:00
abstract:BACKGROUND:Factors predicting the occurrence of premature coronary artery disease (CAD) may not be quantitatively the same as those predicting CAD severity, particularly in women, in whom there have been few studies. METHODS AND RESULTS:To determine factors predictive of severity of CAD and of angina pectoris, we docu...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.89.5.1974
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:Previous studies have reported an association between chronic Helicobacter pylori infection and ischemic heart disease. However, it is not clear whether this association is really due to the virulence of the bacterium or is merely the result of confounding factors (in particular, age and social class). METH...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.97.17.1675
更新日期:1998-05-05 00:00:00
abstract:BACKGROUND:Intermittent claudication (IC) is associated with an increased risk of cardiovascular disease morbidity and mortality. The relation of alcohol consumption to the risk of IC remains controversial. The purpose of this study was to assess the relation of alcohol consumption and type of beverage to the developme...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.25.3092
更新日期:2000-12-19 00:00:00
abstract::Eleven patients underwent exercise testing after operative repair of anomalous origin of the left coronary artery from the pulmonary artery. Five patients repaired after 2 years of age comprised a childhood surgery group, and six patients repaired before 2 years of age comprised an infant surgery group. All patients w...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.81.4.1287
更新日期:1990-04-01 00:00:00
abstract::Survival data after 8 years of follow-up for all patients and after 7 years for certain subgroups are reported from CASS, a randomized trial of surgical or medical treatment assignment in patients with coronary artery disease who have less than severe angina or are asymptomatic after myocardial infarction. After 8 yea...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:
更新日期:1985-12-01 00:00:00
abstract::Current imaging modalities can provide only a qualitative or semiquantitative measure of the severity of aortic regurgitation. Ultrafast computed tomography (CT) has the capability of rapid imaging (17 frames/sec) coupled with high spatial resolution (1.5 mm2). Eight millimeter thick images can be acquired to interrog...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.76.3.728
更新日期:1987-09-01 00:00:00
abstract:BACKGROUND:Factor Xa plays a central role in the generation of thrombin, making it a novel target for treatment of arterial thrombosis. Fondaparinux is a synthetic factor Xa inhibitor that has been shown to be superior to standard therapies for the prevention of venous thrombosis. We performed a randomized trial to det...
journal_title:Circulation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1161/01.CIR.0000158485.70761.67
更新日期:2005-03-22 00:00:00
abstract::Cardiac hypertrophy is the physiological adaptation of the heart to chronic mechanical overload. Cardiac failure indicates the limits of the process. Cardiac hypertrophy is only one example of biological adaptation and results from the induction of several changes in gene expression, mostly of the fetal type, includin...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:Thrombolytic therapy of acute myocardial infarction (AMI) is evolving toward bolus administration. Derivatization of proteins with polyethylene glycol (PEG) may reduce their clearance. METHODS AND RESULTS:A staphylokinase (SakSTAR) variant with 12 amino acid substitutions to reduce its antigenicity, SakSTAR...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.102.15.1766
更新日期:2000-10-10 00:00:00
abstract:BACKGROUND:Biventricular direct cardiac compression (DCC) has the potential to support the failing heart without the complications associated with a blood/device interface encountered with the use of current ventricular assist devices. A clinically designed DCC device that provides compression pressure around the base ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.100.suppl_2.ii-236
更新日期:1999-11-09 00:00:00
abstract::We have monitored ambulant intra-arterial blood pressure with the Oxford system in six subjects with autonomic failure who exhibited postural hypotension. Plotting pooled hourly mean values we have demonstrated a consistent circadian trend in blood pressure that was the inverse of the normal pattern, with the highest ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.68.3.477
更新日期:1983-09-01 00:00:00
abstract:BACKGROUND:Fingolimod, a sphingosine-1-phosphate receptor agonist, is used for the treatment of multiple sclerosis and exerts antiapoptotic properties. We hypothesized that sphingosine-1-phosphate receptor activation with fingolimod during acute myocardial infarction (MI) inhibits apoptosis, leading to increased myocar...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.115.012427
更新日期:2016-03-08 00:00:00
abstract::The existence of a brain renin-angiotensin system (RAS) as one of various tissue RASs is now firmly established. Angiotensin-containing pathways within brain areas involved in central blood pressure regulation have been described. Evidence from biochemical, neurophysiologic, pharmacologic, and most recently, molecular...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:
更新日期:1988-06-01 00:00:00
abstract:BACKGROUND:Isomerism is associated with a complex spectrum of anomalies. There is paucity of data on prenatally detected cases. METHODS AND RESULTS:Between January 1990 and February 2004, 83 of 166 cases (50%) had a prenatal diagnosis of left isomerism (LAI; 52 of 97) or right isomerism (RAI; 31 of 69) at our institut...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.105.552364
更新日期:2005-10-18 00:00:00
abstract:BACKGROUND:Paroxysmal atrial fibrillation (PAF) can be eliminated with continuous circular lesions (CCLs) around the pulmonary veins (PVs), but it is unclear whether all PVs are completely isolated. METHODS AND RESULTS:Forty-one patients with symptomatic PAF underwent 3D mapping, and all PV ostia were marked on the 3D...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000144459.37455.EE
更新日期:2004-10-12 00:00:00
abstract:BACKGROUND:Since the atria are thin-walled structures, most studies that have examined the spread of activation in the atria have assumed that they behave electrophysiologically as a two-dimensional surface. It was the objective of this study to determine whether or not this assumption is true by simultaneously mapping...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.88.1.250
更新日期:1993-07-01 00:00:00
abstract::Two-dimensional echocardiographic findings in a group of 24 patients with atrial septal defects were correlated with findings obtained by cardiac catheterization and M-mode echocardiography. The prevalence of mitral prolapse was 95% by two-dimensional echocardiography and 59% by angiography in patients with secundum a...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.56.3.447
更新日期:1977-09-01 00:00:00