Abstract:
PURPOSE:To analyze and compare the high performance liquid chromatography (HPLC) runs of tear proteins from diabetic (DIDRY) and non-diabetic (DRY) dry-eye patients, and healthy subjects (CTRL). The patterns were analyzed using multivariate statistical methods. METHODS:Tears (total 56 eyes: CTRL: n=16, DIDRY: n=21, and DRY: n=19) were analyzed by HPLC, using a size-exclusion column with an eluent of 0.5 M sodium phosphate buffer. The patients were primarily grouped according to the results of the basic secretory test (BST) in combination with subjective symptoms such as burning, foreign body sensations, tearing, and "dryness" of the eyes. Patients with BST values < or = 10 mm/5 min plus two subjective symptoms were grouped as dry-eye patients. Before statistical analysis, each HPLC run was quantitatively analyzed using ScanPacK software (ScanPacK, Göttingen, Germany), and a data set was created from each HPLC run. The data were then analyzed by multivariate analysis of discriminance. RESULTS:The HPLC patterns of CTRL, DIDRY and DRY were significantly different (Wilks' lambda: 0.0209; p<0.01). The area of the sigA peak was significantly smaller (p<0.05) in dry-eye tears than controls. There was a good correlation between the extent of separation in the multivariate analysis and the BST value (r = -0.71). Classification of all samples resulted in 98% correct assignments. CONCLUSIONS:The analysis of HPLC patterns and subsequent statistical evaluation are useful for the detection of dry eyes. The HPLC method and the statistical routines described allow a shorter analysis time than electrophoresis. HPLC analysis in combination with statistical analysis can be used as a diagnostic tool for the detection of dry eyes, and also improves the quality of analysis of disease-associated tear proteins in clinical research.
journal_name
Eur J Ophthalmoljournal_title
European journal of ophthalmologyauthors
Grus FH,Augustin AJdoi
10.1177/112067210101100105subject
Has Abstractpub_date
2001-01-01 00:00:00pages
19-24issue
1eissn
1120-6721issn
1724-6016journal_volume
11pub_type
杂志文章abstract:PURPOSE:The pupil diameter under low ambient illumination is diagnostically valuable for refractive surgery. The aim of study was to compare the NeurOptics® Pupillometer, Sirius®, and Ocular Wavefront Analyser® in determining scotopic pupil diameter. MATERIALS AND METHODS:A total of 96 eyes of 48 subjects were include...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000150
更新日期:2012-11-01 00:00:00
abstract:PURPOSE:To evaluate the usefulness of non-mydriatic fundus camera (NMFu-camera) and frequency doubling perimeter (FDP) for detecting glaucoma in a general population. METHODS:This prospective observational multicenter study consisted in screening for glaucoma in the populations of three Belgian cities. Intraocular pre...
journal_title:European journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1177/112067210401400505
更新日期:2004-09-01 00:00:00
abstract:PURPOSE:To compare ocular findings in eyes with primary open-angle glaucoma (POAG) and exfoliation glaucoma (EG) at the time of diagnosis when identical diagnostic criteria for glaucoma were applied. METHODS:We conducted a study on records of 519 consecutive, unselected patients with chronic glaucoma filing for cost-f...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000516
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:To examine the nature and frequency of ocular side effects due to systemic target therapy with BRAF and MEK inhibitors as well as immunotherapy with cytotoxic T-lymphocyte antigen 4 (CTLA-4) and programmed cell death 1 (PD-1) monoclonal antibodies used in the treatment of cutaneous malignant melanoma (CMM). DE...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672120930688
更新日期:2020-05-31 00:00:00
abstract:PURPOSE:To evaluate the use of 10/0 polyester (Mersilene) sutures for closure of small corneal incision after congenital cataract surgery. METHODS:The authors retrospectively reviewed the medical records of 58 cases (42 patients) who underwent congenital cataract extraction and intraocular lens implantation between 19...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/112067210801800114
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:To compare between mitomycin C alone, porcine extracellular matrix alone, and combined low dose mitomycin C with porcine extracellular matrix in term of efficacy and safety in phaco-trabeculectomy surgery. STUDY DESIGN:Prospective comparative. METHODS:Sixty eyes of 60 patients complaining of primary open angl...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672120944339
更新日期:2021-01-01 00:00:00
abstract::Cone-rod dystrophy (CORD) is one of the inherited retinal diseases that result in central visual field deterioration and decreased visual acuity (VA). In CORD patients, impaired photoreceptor cells are observed as the disruption of ellipsoid zone (EZ) on optical coherence tomography (OCT) images. In the present study,...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672121990561
更新日期:2021-01-26 00:00:00
abstract:PURPOSE:Parry-Romberg syndrome (PRS) is a rare disease of unknown etiology and pathogenesis, characterized by progressive hemifacial atrophy. Diverse ocular manifestations were reported in association with PRS, including enophthalmos, lid retraction, blepharoptosis, restrictive strabismus, ocular motor nerve dysfunctio...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/112067211002000132
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:To evaluate the effects of levodopa and dopamine agonists on retinal nerve fiber layer using scanning laser ophthalmoscope. METHODS:Forty-four patients with the diagnosis of Parkinson disease and receiving levodopa or dopamine agonist monotherapy were included in this prospective study. The control group consi...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/112067210701700520
更新日期:2007-09-01 00:00:00
abstract:PURPOSE:To evaluate the choroidal vascularity index of eyes for acute and chronic central serous chorioretinopathy patients using swept-source optical coherence tomography generated en-face scans. METHODS:This was a retrospective study, in which slabs of en-face optical coherence tomography scans, at 5 μm intervals, s...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672120908719
更新日期:2020-02-27 00:00:00
abstract:PURPOSE:The authors report the ophthalmic characteristics of a male proband in a Japanese family with autosomal dominant optic atrophy (DOA) harboring a frameshift mutation in the OPA1 gene. METHODS:Conventional ophthalmologic examinations including static automated perimetry were performed, as well as assessment of t...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/112067210701700216
更新日期:2007-03-01 00:00:00
abstract:PURPOSE:To describe a case of traumatic hyphema in a patient with severe hemophilia A. CASE:We present a case of a 16-year-old boy with severe hemophilia A who presented to our ophthalmology department with total hyphema and elevated intraocular pressure 3 days after a history of blunt ocular trauma on his right eye. ...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672119856515
更新日期:2019-06-12 00:00:00
abstract:PURPOSE:Norrie disease (ND) is a rare X-linked recessive disorder characterized by congenital blindness, which is often associated with sensorineural hearing loss and mental retardation. X-linked familial exudative vitreoretinopathy (FEVR) is a hereditary disorder characterized by an abnormality of the peripheral retin...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/112067219900900312
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:Reconstruction of the ocular surface is challenging. As an alternative to mucosal and limbal epithelial, we study the feasibility of cultivated human conjunctival epithelial (HCjE) cells of patients with total limbal stem cell deficiency (LSCD). METHODS:We studied superior forniceal conjunctival biopsies harve...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000511
更新日期:2014-11-06 00:00:00
abstract:OBJECTIVE OF THE STUDY:Inborn lens opacity is the most frequent cause of childhood blindness. In this study, we aimed to define the presumed genetic cause of a congenital cataract present in a Mauritanian family over the last nine generations. METHODS:A family history of the disease and eye examination were carried ou...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672118804757
更新日期:2019-11-01 00:00:00
abstract:PURPOSE:To assess the intraoperative vaulting in patients undergoing implantable collamer lens (ICL) implantation with microscope-integrated intraoperative optical coherence tomography (iOCT) and correlate it with the postoperative vaulting. METHODS:Forty eyes of 22 consecutive patients undergoing ICL implantation wer...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000818
更新日期:2017-01-19 00:00:00
abstract:PURPOSE:To compare the outcomes after switching between bevacizumab and ranibizumab therapy due to poor treatment effect in neovascular age-related macular degeneration (AMD). METHODS:This is a retrospective review of patients with neovascular AMD with first treatment using intravitreal bevacizumab (group 1) or ranibi...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000480
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:This is a report of 2 cases of cutis aplasia and cutis marmorata telangiectasia with associated retinal detachment. METHODS:Retrospective case report. RESULTS:Illustration of ophthalmic associations of the rare congenital dermatologic presentations and description of successful treatment with laser photocoagu...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000137
更新日期:2012-09-01 00:00:00
abstract::A 60-year-old patient with a history of diabetes mellitus and coronary artery disease was admitted to the eye nose and throat clinic due to complaints of difficulty in breathing. The patient was decided for functional endoscopic sinus surgery with the diagnosis of right nasal polyposis and chronic sinusitis. The patie...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672118798570
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To compare the IOP readings of an ocular blood flow (OBF) tonograph (OBF Labs UK Ltd) with Goldmann applanation tonometry. METHODS:194 patients were studied. In group 1, the software version 8.2 of the OBF tonograph was used in 214 eyes of 107 patients and in group 2, the 11.2 version of the tonograph was used...
journal_title:European journal of ophthalmology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1998-07-01 00:00:00
abstract:UNLABELLED:PURPOSE. To report a case of scleral dislocation mimicking glaucoma progression. METHODS:Interventional case report. RESULTS:A 71-year-old man was referred for glaucoma surgery in his right eye because of perimetry defect progression and uncontrolled intraocular pressure despite maximal medical therapy. A ...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000165
更新日期:2013-03-01 00:00:00
abstract:PURPOSE:To evaluate the safety and efficacy of sealed implantation, a simple technique for implanting foldable intraocular lenses (IOLs). METHODS:This was a retrospective study including consecutive patients who underwent phacoemulsification cataract using the sealed implantation technique. Sealed implantation is a te...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000997
更新日期:2017-08-30 00:00:00
abstract:PURPOSE:To evaluate persistency (time on initial therapy) and the clinical impact of latanoprost versus beta-blocker monotherapy in treating glaucoma. METHODS:This observational, multicenter, retrospective medical chart review study conducted in four European countries included patients with primary open-angle glaucom...
journal_title:European journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1177/112067210301304s03
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:To report a patient who sustained a spark fire burn during a routine pterygium operation. METHODS:A 40-year-old woman was referred for pterygium excision in her left eye. During the operation a low temperature cautery caused a spark fire that resulted in left eye corneal burn, upper and lower eyelid burns, and...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/112067210801800425
更新日期:2008-07-01 00:00:00
abstract:PURPOSE:To describe the surgical interventions in congenital pupillary-iris-lens membrane. Six patients with congenital pupillary-iris-lens membrane presented with partial or totally occluded pupils, or increased intraocular pressure (IOP). METHODS:We describe the value of creating 2 paracenteses. The first one is mad...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/EJO.2011.7734
更新日期:2011-09-01 00:00:00
abstract:PURPOSE:Management of Graves' ophthalmopathy remains challenging. Over the last decade, previous studies have shown promising results for Rituximab in the treatment of Graves' ophthalmopathy. We present the response of 14 individuals with active moderate-to-severe Graves' ophthalmopathy to Rituximab, representing one o...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672119845224
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To review the current practice of retreatment with Ozurdex injections in patients with macular edema (ME) secondary to retinal vein occlusion (RVO), and to recommend simple guidelines for Ozurdex reinjection in management of RVO. METHODS:This was a multicenter retrospective study of patients who received more ...
journal_title:European journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.5301/ejo.5000376
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:The present study compared the expression of brain-derived neurotrophic factor (BDNF) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of BDNF in the lateral geniculate body in the pathogenesis of amblyopia. METHODS:Twenty kittens were div...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.1177/1120672120953341
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To present a case of ocular decompression retinopathy occurring after an uncomplicated nonpenetrating glaucoma procedure (deep sclerectomy with mitomycin C). METHODS:A 52-year-old man with medically uncontrolled exfoliation glaucoma in the left eye underwent uneventful deep sclerectomy with mitomycin C. The pa...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/EJO.2010.5731
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:To describe the management with intracameral recombinant tissue plasminogen activator (rtPA) of severe fibrin effusion after phacoemulsification, and its prevention with sustained-release dexamethasone intravitreal implant. METHODS:A 59-year-old man with chronic HLA-B27-associated uveitis underwent phacoemulsi...
journal_title:European journal of ophthalmology
pub_type: 杂志文章
doi:10.5301/ejo.5000442
更新日期:2014-07-01 00:00:00