High performance liquid chromatography analysis of tear protein patterns in diabetic and non-diabetic dry-eye patients.

Abstract:

PURPOSE:To analyze and compare the high performance liquid chromatography (HPLC) runs of tear proteins from diabetic (DIDRY) and non-diabetic (DRY) dry-eye patients, and healthy subjects (CTRL). The patterns were analyzed using multivariate statistical methods. METHODS:Tears (total 56 eyes: CTRL: n=16, DIDRY: n=21, and DRY: n=19) were analyzed by HPLC, using a size-exclusion column with an eluent of 0.5 M sodium phosphate buffer. The patients were primarily grouped according to the results of the basic secretory test (BST) in combination with subjective symptoms such as burning, foreign body sensations, tearing, and "dryness" of the eyes. Patients with BST values < or = 10 mm/5 min plus two subjective symptoms were grouped as dry-eye patients. Before statistical analysis, each HPLC run was quantitatively analyzed using ScanPacK software (ScanPacK, Göttingen, Germany), and a data set was created from each HPLC run. The data were then analyzed by multivariate analysis of discriminance. RESULTS:The HPLC patterns of CTRL, DIDRY and DRY were significantly different (Wilks' lambda: 0.0209; p<0.01). The area of the sigA peak was significantly smaller (p<0.05) in dry-eye tears than controls. There was a good correlation between the extent of separation in the multivariate analysis and the BST value (r = -0.71). Classification of all samples resulted in 98% correct assignments. CONCLUSIONS:The analysis of HPLC patterns and subsequent statistical evaluation are useful for the detection of dry eyes. The HPLC method and the statistical routines described allow a shorter analysis time than electrophoresis. HPLC analysis in combination with statistical analysis can be used as a diagnostic tool for the detection of dry eyes, and also improves the quality of analysis of disease-associated tear proteins in clinical research.

journal_name

Eur J Ophthalmol

authors

Grus FH,Augustin AJ

doi

10.1177/112067210101100105

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

19-24

issue

1

eissn

1120-6721

issn

1724-6016

journal_volume

11

pub_type

杂志文章
  • Comparison of 3 pupillometers for determining scotopic pupil diameter.

    abstract:PURPOSE:The pupil diameter under low ambient illumination is diagnostically valuable for refractive surgery. The aim of study was to compare the NeurOptics® Pupillometer, Sirius®, and Ocular Wavefront Analyser® in determining scotopic pupil diameter. MATERIALS AND METHODS:A total of 96 eyes of 48 subjects were include...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000150

    authors: Altan C,Kaya V,Basarir B,Celik U,Azman E,Akar S,Demirok A,Yilmaz OF

    更新日期:2012-11-01 00:00:00

  • Screening for glaucoma in a general population with the non-mydriatic fundus camera and the frequency doubling perimeter.

    abstract:PURPOSE:To evaluate the usefulness of non-mydriatic fundus camera (NMFu-camera) and frequency doubling perimeter (FDP) for detecting glaucoma in a general population. METHODS:This prospective observational multicenter study consisted in screening for glaucoma in the populations of three Belgian cities. Intraocular pre...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章,多中心研究

    doi:10.1177/112067210401400505

    authors: Detry-Morel M,Zeyen T,Kestelyn P,Collignon J,Goethals M,Belgian Glaucoma Society.

    更新日期:2004-09-01 00:00:00

  • Comparison of primary open-angle glaucoma and exfoliation glaucoma at diagnosis.

    abstract:PURPOSE:To compare ocular findings in eyes with primary open-angle glaucoma (POAG) and exfoliation glaucoma (EG) at the time of diagnosis when identical diagnostic criteria for glaucoma were applied. METHODS:We conducted a study on records of 519 consecutive, unselected patients with chronic glaucoma filing for cost-f...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000516

    authors: Tarkkanen AH,Kivelä TT

    更新日期:2015-03-01 00:00:00

  • Ocular side effects of target therapy and immunotherapy in patients with cutaneous malignant melanoma.

    abstract:PURPOSE:To examine the nature and frequency of ocular side effects due to systemic target therapy with BRAF and MEK inhibitors as well as immunotherapy with cytotoxic T-lymphocyte antigen 4 (CTLA-4) and programmed cell death 1 (PD-1) monoclonal antibodies used in the treatment of cutaneous malignant melanoma (CMM). DE...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672120930688

    authors: Eikenberry J,Harris A,Torabi R,Lang M,Denney D,Verticchio Vercellin A,Siesky B

    更新日期:2020-05-31 00:00:00

  • The removal of 10/0 polyester (Mersilene) sutures after small incision congenital cataract surgery.

    abstract:PURPOSE:To evaluate the use of 10/0 polyester (Mersilene) sutures for closure of small corneal incision after congenital cataract surgery. METHODS:The authors retrospectively reviewed the medical records of 58 cases (42 patients) who underwent congenital cataract extraction and intraocular lens implantation between 19...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/112067210801800114

    authors: Bar-Sela SM,Spierer O,Spierer A

    更新日期:2008-01-01 00:00:00

  • The role of mitomycin C and porcine extracellular matrix in phacotrabeculectomy.

    abstract:PURPOSE:To compare between mitomycin C alone, porcine extracellular matrix alone, and combined low dose mitomycin C with porcine extracellular matrix in term of efficacy and safety in phaco-trabeculectomy surgery. STUDY DESIGN:Prospective comparative. METHODS:Sixty eyes of 60 patients complaining of primary open angl...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672120944339

    authors: Hamdi MM,Hamdi IM

    更新日期:2021-01-01 00:00:00

  • Quantification of residual ellipsoid zone and its correlation with visual functions in patients with cone-rod dystrophy.

    abstract::Cone-rod dystrophy (CORD) is one of the inherited retinal diseases that result in central visual field deterioration and decreased visual acuity (VA). In CORD patients, impaired photoreceptor cells are observed as the disruption of ellipsoid zone (EZ) on optical coherence tomography (OCT) images. In the present study,...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672121990561

    authors: Hara T,Zhou HP,Kitano M,Kure K,Asaoka R,Inoue T,Obata R

    更新日期:2021-01-26 00:00:00

  • Unilateral optic atrophy preceding Coats disease in a girl with Parry-Romberg syndrome.

    abstract:PURPOSE:Parry-Romberg syndrome (PRS) is a rare disease of unknown etiology and pathogenesis, characterized by progressive hemifacial atrophy. Diverse ocular manifestations were reported in association with PRS, including enophthalmos, lid retraction, blepharoptosis, restrictive strabismus, ocular motor nerve dysfunctio...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/112067211002000132

    authors: Nasser O,Greiner K,Amer R

    更新日期:2010-01-01 00:00:00

  • The effect of levodopa and dopamine agonists on optic nerve head in Parkinson disease.

    abstract:PURPOSE:To evaluate the effects of levodopa and dopamine agonists on retinal nerve fiber layer using scanning laser ophthalmoscope. METHODS:Forty-four patients with the diagnosis of Parkinson disease and receiving levodopa or dopamine agonist monotherapy were included in this prospective study. The control group consi...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/112067210701700520

    authors: Yavas GF,Yilmaz O,Küsbeci T,Oztürk F

    更新日期:2007-09-01 00:00:00

  • En-face choroidal vascularity in central serous chorioretinopathy.

    abstract:PURPOSE:To evaluate the choroidal vascularity index of eyes for acute and chronic central serous chorioretinopathy patients using swept-source optical coherence tomography generated en-face scans. METHODS:This was a retrospective study, in which slabs of en-face optical coherence tomography scans, at 5 μm intervals, s...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672120908719

    authors: Wong RL,Singh SR,Rasheed MA,Goud A,Chhablani G,Samantaray S,AnkiReddy S,Vupparaboina KK,Chhablani J

    更新日期:2020-02-27 00:00:00

  • Dominant optic atrophy in a Japanese family with OPA1 frameshift mutation (V942fsX966).

    abstract:PURPOSE:The authors report the ophthalmic characteristics of a male proband in a Japanese family with autosomal dominant optic atrophy (DOA) harboring a frameshift mutation in the OPA1 gene. METHODS:Conventional ophthalmologic examinations including static automated perimetry were performed, as well as assessment of t...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/112067210701700216

    authors: Hayashi T,Takeuchi T,Gekka T,Kitahara K

    更新日期:2007-03-01 00:00:00

  • Traumatic hyphema in a patient with severe hemophilia A: Clinical features and management.

    abstract:PURPOSE:To describe a case of traumatic hyphema in a patient with severe hemophilia A. CASE:We present a case of a 16-year-old boy with severe hemophilia A who presented to our ophthalmology department with total hyphema and elevated intraocular pressure 3 days after a history of blunt ocular trauma on his right eye. ...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672119856515

    authors: Belviranli S,Ozkagnici A,Tokgoz H,Bitirgen G,Caliskan U

    更新日期:2019-06-12 00:00:00

  • Norrie disease and exudative vitreoretinopathy in families with affected female carriers.

    abstract:PURPOSE:Norrie disease (ND) is a rare X-linked recessive disorder characterized by congenital blindness, which is often associated with sensorineural hearing loss and mental retardation. X-linked familial exudative vitreoretinopathy (FEVR) is a hereditary disorder characterized by an abnormality of the peripheral retin...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/112067219900900312

    authors: Shastry BS,Hiraoka M,Trese DC,Trese MT

    更新日期:1999-07-01 00:00:00

  • Conjunctival epithelial cells cultivated ex vivo from patients with total limbal stem cell deficiency.

    abstract:PURPOSE:Reconstruction of the ocular surface is challenging. As an alternative to mucosal and limbal epithelial, we study the feasibility of cultivated human conjunctival epithelial (HCjE) cells of patients with total limbal stem cell deficiency (LSCD). METHODS:We studied superior forniceal conjunctival biopsies harve...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000511

    authors: Silber PC,Ricardo JR,Cristovam PC,Hazarbassanov RM,Dreyfuss JL,Gomes JA

    更新日期:2014-11-06 00:00:00

  • A novel missense mutation of GJA8 causes congenital cataract in a large Mauritanian family.

    abstract:OBJECTIVE OF THE STUDY:Inborn lens opacity is the most frequent cause of childhood blindness. In this study, we aimed to define the presumed genetic cause of a congenital cataract present in a Mauritanian family over the last nine generations. METHODS:A family history of the disease and eye examination were carried ou...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672118804757

    authors: Hadrami M,Bonnet C,Veten F,Zeitz C,Condroyer C,Wang P,Biya M,Sidi Ahmed MA,Zhang Q,Cheikh S,Audo I,Petit C,Houmeida A

    更新日期:2019-11-01 00:00:00

  • Real-time assessment of intraoperative vaulting in implantable collamer lens and correlation with postoperative vaulting.

    abstract:PURPOSE:To assess the intraoperative vaulting in patients undergoing implantable collamer lens (ICL) implantation with microscope-integrated intraoperative optical coherence tomography (iOCT) and correlate it with the postoperative vaulting. METHODS:Forty eyes of 22 consecutive patients undergoing ICL implantation wer...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000818

    authors: Titiyal JS,Kaur M,Sahu S,Sharma N,Sinha R

    更新日期:2017-01-19 00:00:00

  • Switching intravitreal anti-VEGF treatment in neovascular age-related macular degeneration.

    abstract:PURPOSE:To compare the outcomes after switching between bevacizumab and ranibizumab therapy due to poor treatment effect in neovascular age-related macular degeneration (AMD). METHODS:This is a retrospective review of patients with neovascular AMD with first treatment using intravitreal bevacizumab (group 1) or ranibi...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000480

    authors: Küçükerdönmez C,Gelisken F,Yoeruek E,Bartz-Schmidt KU,Leitritz MA

    更新日期:2015-01-01 00:00:00

  • Pediatric retinal detachment in cutis aplasia and cutis marmorata telangiectasia.

    abstract:PURPOSE:This is a report of 2 cases of cutis aplasia and cutis marmorata telangiectasia with associated retinal detachment. METHODS:Retrospective case report. RESULTS:Illustration of ophthalmic associations of the rare congenital dermatologic presentations and description of successful treatment with laser photocoagu...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000137

    authors: Lagan M,Brennan R,McLoone E

    更新日期:2012-09-01 00:00:00

  • A rare complication of endoscopic sinus surgery: Retinal artery occlusion.

    abstract::A 60-year-old patient with a history of diabetes mellitus and coronary artery disease was admitted to the eye nose and throat clinic due to complaints of difficulty in breathing. The patient was decided for functional endoscopic sinus surgery with the diagnosis of right nasal polyposis and chronic sinusitis. The patie...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672118798570

    authors: Kurt MM,Akpolat C,Fethallah B

    更新日期:2019-05-01 00:00:00

  • Clinical comparison of the ocular blood flow tonograph and the Goldmann applanation tonometer.

    abstract:PURPOSE:To compare the IOP readings of an ocular blood flow (OBF) tonograph (OBF Labs UK Ltd) with Goldmann applanation tonometry. METHODS:194 patients were studied. In group 1, the software version 8.2 of the OBF tonograph was used in 214 eyes of 107 patients and in group 2, the 11.2 version of the tonograph was used...

    journal_title:European journal of ophthalmology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Esgin H,Alimgil ML,Erda S

    更新日期:1998-07-01 00:00:00

  • Scleral buckling dislocation mimicking glaucoma progression.

    abstract:UNLABELLED:PURPOSE. To report a case of scleral dislocation mimicking glaucoma progression. METHODS:Interventional case report. RESULTS:A 71-year-old man was referred for glaucoma surgery in his right eye because of perimetry defect progression and uncontrolled intraocular pressure despite maximal medical therapy. A ...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000165

    authors: Codenotti M,Iuliano L,Fogliato G,De Benedetto U,Bandello F

    更新日期:2013-03-01 00:00:00

  • Sealed implantation of foldable intraocular lenses.

    abstract:PURPOSE:To evaluate the safety and efficacy of sealed implantation, a simple technique for implanting foldable intraocular lenses (IOLs). METHODS:This was a retrospective study including consecutive patients who underwent phacoemulsification cataract using the sealed implantation technique. Sealed implantation is a te...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000997

    authors: Nath D,Blumenthal EZ,Sharma M,Mimouni M

    更新日期:2017-08-30 00:00:00

  • Persistency and clinical outcomes associated with latanoprost and beta-blocker monotherapy: evidence from a European retrospective cohort study.

    abstract:PURPOSE:To evaluate persistency (time on initial therapy) and the clinical impact of latanoprost versus beta-blocker monotherapy in treating glaucoma. METHODS:This observational, multicenter, retrospective medical chart review study conducted in four European countries included patients with primary open-angle glaucom...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章,多中心研究

    doi:10.1177/112067210301304s03

    authors: Diestelhorst M,Schaefer CP,Beusterien KM,Plante KM,Fain JM,Mozaffari E,Dhawan R

    更新日期:2003-07-01 00:00:00

  • Burn during a routine pterygium excision operation.

    abstract:PURPOSE:To report a patient who sustained a spark fire burn during a routine pterygium operation. METHODS:A 40-year-old woman was referred for pterygium excision in her left eye. During the operation a low temperature cautery caused a spark fire that resulted in left eye corneal burn, upper and lower eyelid burns, and...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/112067210801800425

    authors: Naftali M,Jabaly-Habib H,Mukari A

    更新日期:2008-07-01 00:00:00

  • Surgical management of congenital pupillary-iris-lens membrane.

    abstract:PURPOSE:To describe the surgical interventions in congenital pupillary-iris-lens membrane. Six patients with congenital pupillary-iris-lens membrane presented with partial or totally occluded pupils, or increased intraocular pressure (IOP). METHODS:We describe the value of creating 2 paracenteses. The first one is mad...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/EJO.2011.7734

    authors: Yu T,Yin ZQ,Yang H

    更新日期:2011-09-01 00:00:00

  • The effects of Rituximab on Graves'orbitopathy: A retrospective study of 14 patients.

    abstract:PURPOSE:Management of Graves' ophthalmopathy remains challenging. Over the last decade, previous studies have shown promising results for Rituximab in the treatment of Graves' ophthalmopathy. We present the response of 14 individuals with active moderate-to-severe Graves' ophthalmopathy to Rituximab, representing one o...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672119845224

    authors: Eid L,Coste-Verdier V,Longueville E,Ribeiro E,Nicolescu-Catargi B,Korobelnik JF

    更新日期:2020-09-01 00:00:00

  • Retreatment with Ozurdex for macular edema secondary to retinal vein occlusion.

    abstract:PURPOSE:To review the current practice of retreatment with Ozurdex injections in patients with macular edema (ME) secondary to retinal vein occlusion (RVO), and to recommend simple guidelines for Ozurdex reinjection in management of RVO. METHODS:This was a multicenter retrospective study of patients who received more ...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章,多中心研究

    doi:10.5301/ejo.5000376

    authors: Coscas G,Augustin A,Bandello F,de Smet MD,Lanzetta P,Staurenghi G,Parravano MC,Udaondo P,Moisseiev E,Soubrane G,Yatziv Y,Loewenstein A

    更新日期:2014-01-01 00:00:00

  • Expression of brain-derived neurotrophic factor in the lateral geniculate body of monocular form deprivation amblyopic kittens.

    abstract:PURPOSE:The present study compared the expression of brain-derived neurotrophic factor (BDNF) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of BDNF in the lateral geniculate body in the pathogenesis of amblyopia. METHODS:Twenty kittens were div...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.1177/1120672120953341

    authors: Li B,Zou Y,Yin X,Tang X,Fan H

    更新日期:2020-09-01 00:00:00

  • Ocular decompression retinopathy after deep sclerectomy with mitomycin C in an eye with exfoliation glaucoma.

    abstract:PURPOSE:To present a case of ocular decompression retinopathy occurring after an uncomplicated nonpenetrating glaucoma procedure (deep sclerectomy with mitomycin C). METHODS:A 52-year-old man with medically uncontrolled exfoliation glaucoma in the left eye underwent uneventful deep sclerectomy with mitomycin C. The pa...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/EJO.2010.5731

    authors: Kozobolis VP,Kalogianni E,Katsanos A,Dardabounis D,Koukoula S,Labiris G

    更新日期:2011-05-01 00:00:00

  • Fibrin reaction after uveitic cataract surgery: treatment and prevention.

    abstract:PURPOSE:To describe the management with intracameral recombinant tissue plasminogen activator (rtPA) of severe fibrin effusion after phacoemulsification, and its prevention with sustained-release dexamethasone intravitreal implant. METHODS:A 59-year-old man with chronic HLA-B27-associated uveitis underwent phacoemulsi...

    journal_title:European journal of ophthalmology

    pub_type: 杂志文章

    doi:10.5301/ejo.5000442

    authors: da Rocha Lima B,Pichi F,Nucci P,Srivastava SK,Lowder CY

    更新日期:2014-07-01 00:00:00