Abstract:
:In order to understand the role of the glycans in glycoproteins in solution, structural information obtained by NMR spectroscopy is obviously required. However, the assignment of the NMR signals from the glycans in larger glycoproteins is still difficult, mainly due to the lack of appropriate methods for the assignment of the resonances originating from the glycans. By using [U-13C6, 2H7]glucose as a metabolic precursor, we have successfully prepared a glycoprotein whose glycan is uniformly labeled with 13C and partially with D at the sugar residues. The D to H exchange ratios at the C1-C6 positions of the sugar residues have been proven to provide useful information for the spectral assignments of the glycan in the glycoprotein. This is the first report on the residue-specific assignment of the anomeric resonances originating from a glycan attached to a glycoprotein by using the metabolic incorporation of hydrogen from the medium into a glycan labeled with [U-13C6, 2H7]glucose.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Yamaguchi Y,Takizawa T,Kato K,Arata Y,Shimada Idoi
10.1023/a:1026776721348subject
Has Abstractpub_date
2000-12-01 00:00:00pages
357-60issue
4eissn
0925-2738issn
1573-5001journal_volume
18pub_type
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