Optimization of 1H decoupling eliminates sideband artifacts in 3D TROSY-based triple resonance experiments.


:TROSY-based triple resonance experiments are essential for protein backbone assignment of large biomolecular systems by solution NMR spectroscopy. In a survey of the current Bruker pulse sequence library for TROSY-based experiments we found that several sequences were plagued by artifacts that affect spectral quality and hamper data analysis. Specifically, these experiments produce sidebands in the 13C(t 1) dimension with inverted phase corresponding to 1HN resonance frequencies, with approximately 5% intensity of the parent 13C crosspeaks. These artifacts originate from the modulation of the 1HN frequency onto the resonance frequency of 13Cα and/or 13Cβ and are due to 180° pulses imperfections used for 1H decoupling during the 13C(t 1) evolution period. These sidebands can become severe for CAi, CAi-1 and/or CBi, CBi-1 correlation experiments such as TROSY-HNCACB. Here, we implement three alternative decoupling strategies that suppress these artifacts and, depending on the scheme employed, boost the sensitivity up to 14% on Bruker spectrometers. A class of comparable Agilent/Varian pulse sequences that use WALTZ16 1H decoupling can also be improved by this method resulting in up to 60-80% increase in sensitivity.


J Biomol NMR


Xia Y,Rossi P,Tonelli M,Huang C,Kalodimos CG,Veglia G




Has Abstract


2017-09-01 00:00:00














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    abstract::The binding site and backbone dynamics of a bioactive complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed heparin hexasaccharide has been investigated by HSQC and relaxation NMR methods. The comparison of the relaxation data for the free and bound states has allowed showing that t...

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    pub_type: 杂志文章


    authors: Canales-Mayordomo A,Fayos R,Angulo J,Ojeda R,Martín-Pastor M,Nieto PM,Martín-Lomas M,Lozano R,Giménez-Gallego G,Jiménez-Barbero J

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  • Automated 1H and 13C chemical shift prediction using the BioMagResBank.

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    journal_title:Journal of biomolecular NMR

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    pub_type: 杂志文章


    authors: Piai A,Gonnelli L,Felli IC,Pierattelli R,Kazimierczuk K,Grudziąż K,Koźmiński W,Zawadzka-Kazimierczuk A

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    journal_title:Journal of biomolecular NMR

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    abstract::In 2HJ(NN)-COSY experiments, which correlate protons with donor/acceptor nitrogens across Nd...HNa bonds, the receptor nitrogen needs to be assigned in order to unambiguously identify the hydrogen bond. For many situations this is a non-trivial task which is further complicated by poor dispersion of (Na,Nd) resonances...

    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Majumdar A,Gosser Y,Patel DJ

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  • An improved double-tuned and isotope-filtered pulse scheme based on a pulsed field gradient and a wide-band inversion shaped pulse.

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Ogura K,Terasawa H,Inagaki F

    更新日期:1996-12-01 00:00:00

  • Simultaneous detection of intra- and inter-molecular paramagnetic relaxation enhancements in protein complexes.

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Olivieri C,Subrahmanian MV,Xia Y,Kim J,Porcelli F,Veglia G

    更新日期:2018-03-01 00:00:00

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Jin C,Prompers JJ,Brüschweiler R

    更新日期:2003-07-01 00:00:00

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    abstract::Limitations in the applicability, accuracy, and precision of individual structure characterization methods can sometimes be overcome via an integrative modeling approach that relies on information from all available sources, including all available experimental data and prior models. The open-source Integrative Modeli...

    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Vallat B,Webb B,Westbrook J,Sali A,Berman HM

    更新日期:2019-07-01 00:00:00

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Pelupessy P,Ravindranathan S,Bodenhausen G

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Hsiao YW,Drakenberg T,Ryde U

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    abstract::This study presents the first application of the model-free analysis (MFA) (Meiler in J Am Chem Soc 123:6098-6107, 2001; Lakomek in J Biomol NMR 34:101-115, 2006) to methyl group RDCs measured in 13 different alignment media in order to describe their supra-tau (c) dynamics in ubiquitin. Our results indicate that meth...

    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Farès C,Lakomek NA,Walter KF,Frank BT,Meiler J,Becker S,Griesinger C

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Ishima R,Yamasaki K,Saito M,Nagayama K

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    journal_title:Journal of biomolecular NMR

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    authors: Vallurupalli P,Hansen DF,Lundström P,Kay LE

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    authors: Björnerås J,Botana A,Morris GA,Nilsson M

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    authors: Wu PS,Ozawa K,Jergic S,Su XC,Dixon NE,Otting G

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Groth M,Malicka J,Czaplewski C,Ołdziej S,Lankiewicz L,Wiczk W,Liwo A

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    authors: Liao SY,Lee M,Wang T,Sergeyev IV,Hong M

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    journal_title:Journal of biomolecular NMR

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    authors: Lee M,Hong M

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    journal_title:Journal of biomolecular NMR

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    authors: Zawadzka-Kazimierczuk A,Koźmiński W,Billeter M

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    authors: Böckmann A,Lange A,Galinier A,Luca S,Giraud N,Juy M,Heise H,Montserret R,Penin F,Baldus M

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    authors: Niklasson M,Otten R,Ahlner A,Andresen C,Schlagnitweit J,Petzold K,Lundström P

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    journal_title:Journal of biomolecular NMR

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    authors: Robertson IM,Boyko RF,Sykes BD

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Sobol AG,Arseniev AS,Abdulaeva GV,Musina LYu,Bystrov VF

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Ishima R,Louis JM,Torchia DA

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: McCoy MA,Dellwo MJ,Schneider DM,Banks TM,Falvo J,Vavra KJ,Mathiowetz AM,Qoronfleh MW,Ciccarelli R,Cook ER,Pulvino TA,Wahl RC,Wang H

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Dasgupta S,Hu X,Keizers PH,Liu WM,Luchinat C,Nagulapalli M,Overhand M,Parigi G,Sgheri L,Ubbink M

    更新日期:2011-11-01 00:00:00

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Waudby CA,Ouvry M,Davis B,Christodoulou J

    更新日期:2020-01-01 00:00:00

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Mikula KM,Krumwiede L,Plückthun A,Iwaï H

    更新日期:2018-08-01 00:00:00

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    journal_title:Journal of biomolecular NMR

    pub_type: 杂志文章


    authors: Pervushin K

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