Abstract:
BACKGROUND:Administration of angiogenic factors stimulates neovascularization in ischemic tissues. However, there is no evidence that angiogenesis can be induced in normoperfused skeletal muscles. We tested the hypothesis that adenovirus-mediated intramuscular (IM) gene transfer of the 121-amino-acid form of vascular endothelial growth factor (AdCMV.VEGF(121)) could stimulate neovascularization in nonischemic skeletal muscle and consequently attenuate the hemodynamic deficit secondary to surgically induced ischemia. METHODS AND RESULTS:Rabbits and rats received IM injections of AdCMV.VEGF(121), AdCMV.Null, or saline in the thigh, 4 weeks (rabbits) or 2 weeks (rats) before femoral artery removal in the injected limb. In unoperated rats, at the site of injection of AdCMV.VEGF(121), we found 96% and 29% increases in length density of arterioles and capillaries, respectively. Increased tissue perfusion (TP) to the ischemic limb in the AdCMV.VEGF(121) group was documented, as early as day 1 after surgery, by improved blood flow to the ischemic gastrocnemius muscle measured by radioactive microspheres (AdCMV.VEGF(121)=5.69+/-0.40, AdCMV.Null=2.97+/-0.50, and saline=2.78+/-0.43 mL x min(-1) x 100 g(-1), P<0.001), more angiographically recognizable collateral vessels (angioscore) (AdCMV. VEGF(121)=50.58+/-1.48, AdCMV.Null=29.08+/-4.22, saline=11.83+/-1.90, P<0.0001), and improvement of the bioenergetic reserve of the gastrocnemius muscle as assessed by (31)P NMR spectroscopy. Follow-up studies showed that superior TP to the ischemic limb in the AdCMV.VEGF(121) group persisted until it was equalized by spontaneous collateral vessel development in untreated animals. CONCLUSIONS:IM administration of AdCMV.VEGF(121) stimulates angiogenesis in normoperfused skeletal muscles, and the newly formed vessels preserve TP after induction of ischemia.
journal_name
Circulationjournal_title
Circulationauthors
Gowdak LH,Poliakova L,Wang X,Kovesdi I,Fishbein KW,Zacheo A,Palumbo R,Straino S,Emanueli C,Marrocco-Trischitta M,Lakatta EG,Anversa P,Spencer RG,Talan M,Capogrossi MCdoi
10.1161/01.cir.102.5.565subject
Has Abstractpub_date
2000-08-01 00:00:00pages
565-71issue
5eissn
0009-7322issn
1524-4539journal_volume
102pub_type
杂志文章相关文献
CIRCULATION文献大全abstract::The anatomy of 58 specimens of aortic outflow tract atresia was studied. All cases had situs solitus and levocardia, 37 had atrioventricular (AV) concordance, two had common inlet to a right ventricle and 19 had mitral atresia. The great arteries were normally interrelated in all cases. Fifty-one cases had an intact v...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.59.1.173
更新日期:1979-01-01 00:00:00
abstract:BACKGROUND:Functional mitral regurgitation (MR) is caused by systolic traction on the mitral leaflets related to ventricular distortion. Little is known about whether chronic tethering causes the mitral leaflet area to adapt to the geometric needs imposed by tethering, in part because of inability to reconstruct leafle...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.107.749440
更新日期:2008-08-19 00:00:00
abstract:BACKGROUND:Recent findings suggest a causative role of infections in the pathogenesis of atherosclerosis. In hypothesizing an association between infectious agents and the development of atherosclerosis, we would expect a correlation to the extent of atherosclerosis. Moreover, this effect could be multiplied by the num...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/hc0102.101362
更新日期:2002-01-01 00:00:00
abstract::We studied six patients who suffered from angina pectoris but had angiographically patent major coronary arteries. Two of the patients suffered also from congestive heart failure. Three patients had supraventricular tachyarrhythmias. Three patients had conduction disturbances. During coronary angiography the patients ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.74.5.964
更新日期:1986-11-01 00:00:00
abstract:BACKGROUND:The quantity and intensity of physical activity required for the primary prevention of coronary heart disease (CHD) remain unclear. Therefore, we examined the association of the quantity and intensity of physical activity with CHD risk and the impact of other coronary risk factors. METHODS AND RESULTS:We fo...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.9.975
更新日期:2000-08-29 00:00:00
abstract:BACKGROUND:Isoprostanes (iPs) are free radical-catalyzed products of arachidonic acid that reflect lipid peroxidation in vivo. Several iPs exert biological effects in vitro and may contribute to the functional consequences of oxidant stress. For example, iPF(2alpha)-III (8-iso PGF(2alpha)) and iPE(2)-III modulate plate...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.101.24.2833
更新日期:2000-06-20 00:00:00
abstract:BACKGROUND:The metabolic syndrome is associated with increased angiotensin II activity, induction of a proinflammatory and oxidative state, and endothelial dysfunction. We evaluated the ability of irbesartan, an angiotensin receptor blocker, and lipoic acid, an antioxidant, to affect endothelial function and inflammati...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.CIR.0000153272.48711.B9
更新日期:2005-01-25 00:00:00
abstract:BACKGROUND:Continuous-flow rotary pumps with axial design are increasingly used for left ventricular assist support. The efficacy of this design compared with pulsatile, volume displacement pumps, with respect to characteristics of left ventricular unloading, and exercise performance remains largely unstudied. METHODS...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.106.677898
更新日期:2007-09-11 00:00:00
abstract:BACKGROUND:Few data are available on the long-term outcome of patients who undergo laser-assisted balloon angioplasty for recanalization of occluded peripheral arteries. Because the cost of laser angioplasty is high, the value of the method should be carefully analyzed before it can be considered a routine method for r...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.83.3.787
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:Coronary artery disease (CAD) and myocardial infarction (MI) are caused in part by genetic factors. Recently, the MEF2A gene was linked to MI/CAD in a single pedigree with autosomal-dominant pattern of inheritance. In addition, genetic variants within the gene have been associated with MI in case-control set...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.107.728485
更新日期:2008-01-15 00:00:00
abstract:BACKGROUND:Increased carotid intimal-medial thickness (IMT) and coronary artery calcification (CAC) are used as 2 markers of early atherosclerosis. Our objectives were to assess whether increased IMT and CAC are related and to determine the relationship between cardiovascular risk factors and carotid IMT in young adult...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.100.8.838
更新日期:1999-08-24 00:00:00
abstract:BACKGROUND:Dysregulation of adipocyte-derived bioactive molecules plays an important role in the development of atherosclerosis. We previously reported that adiponectin, an adipocyte-specific plasma protein, accumulated in the injured artery from the plasma and suppressed endothelial inflammatory response and vascular ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.0000042707.50032.19
更新日期:2002-11-26 00:00:00
abstract:BACKGROUND:During the development of heart failure (HF), the chief myocardial energy substrate switches from fatty acids to glucose. This metabolic switch, which recapitulates fetal cardiac energy substrate preferences, is thought to maintain aerobic energetic balance. The regulatory mechanisms involved in this metabol...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.94.11.2837
更新日期:1996-12-01 00:00:00
abstract::To determine whether alteration of intrinsic myocardial stiffness is responsible for the reduction of left ventricular filling pressure and volume by nifedipine in patients with impaired baseline ventricular function, we evaluated the hemodynamic responses in 32 patients undergoing diagnostic cardiac catheterization. ...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.cir.74.1.126
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND:Clinical studies have implicated preinfarct angina (brief antecedent ischemia/reperfusion [I/R]) as a predictor of more rapid thrombolysis and lower rates of reocclusion. However, the effects of antecedent ischemia on the efficacy of thrombolysis have not been rigorously assessed. Using a canine model of cor...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.1.88
更新日期:2000-07-04 00:00:00
abstract:BACKGROUND:Patients surviving ventricular fibrillation (VF) or sustained ventricular tachycardia (VT) are at a high risk of death due to a recurrence of arrhythmia. The implantable cardioverter defibrillator (ICD) terminates VT or VF, but it is not known whether this device prolongs life in these patients compared with...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.cir.101.11.1297
更新日期:2000-03-21 00:00:00
abstract::Atherosclerotic cardiovascular disease results in >19 million deaths annually, and coronary heart disease accounts for the majority of this toll. Despite major advances in treatment of coronary heart disease patients, a large number of victims of the disease who are apparently healthy die suddenly without prior sympto...
journal_title:Circulation
pub_type: 共识发展会议,杂志文章,评审
doi:10.1161/01.CIR.0000087481.55887.C9
更新日期:2003-10-14 00:00:00
abstract:BACKGROUND:Bias in studies of preventive medications can occur when healthier patients are more likely to initiate and adhere to therapy than less healthy patients. We sought evidence of this bias by examining associations between statin exposure and various outcomes that should not be causally affected by statin expos...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.108.824151
更新日期:2009-04-21 00:00:00
abstract::All of the electrophysiological mechanisms of arrhythmias are sensitive to the influences of the autonomic nervous system, particularly to its adrenergic limb. Arrhythmogenic areas may also be dependent on the inhomogeneity of these influences because of their pathophysiological structure and/or the neurogenic or humo...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:
更新日期:1991-04-01 00:00:00
abstract::The clinical and pathologic features of 18 consecutive patients with peripartum cardiomyopathy at The Johns Hopkins Hospital were examined in an attempt to define the incidence of myocarditis and to determine its response to immunosuppressive agents. In addition to routine studies, patients were evaluated with echocar...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.81.3.922
更新日期:1990-03-01 00:00:00
abstract::Experimental studies have suggested that electrocardiographic recognition of left ventricular hypertrophy depends on geometric relationships involving wall thickness and chamber size. To determine the clinical significance of these observations, we studied the effects of echocardiographic LV mass (LVM), posterior wall...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.67.4.907
更新日期:1983-04-01 00:00:00
abstract:BACKGROUND:The implantable left ventricular assist device (LVAD) was designed to provide circulatory support as an alternative to heart transplantation or to continued medical therapy of end-stage heart failure. Initial experience with the implantable LVAD used as a bridge to heart transplantation provides a clinical o...
journal_title:Circulation
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1994-11-01 00:00:00
abstract:BACKGROUND:The release of endothelium-derived relaxing factors has been shown experimentally to be of pivotal importance for the maintenance of coronary blood flow during increased demand. In humans with coronary atherosclerosis, endothelial vasodilator dysfunction is not confined only to epicardial conductance vessels...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.9.2345
更新日期:1995-05-01 00:00:00
abstract::Two hundred forty-five patients with variant angina were followed for an average of 80.5 months (range, 36-184 months). Survival rate at 1, 3, 5, and 10 years was 98%, 97%, 97%, and 93%, respectively. Survival rate without myocardial infarction at 1, 3, 5, and 10 years was 86%, 85%, 83%, and 81%, respectively. By univ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.78.1.1
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND:The outcome of aortic valve replacement in patients with severe aortic stenosis, low transvalvular gradient, and severe left ventricular dysfunction is not well known. METHODS AND RESULTS:Between 1985 and 1995, 52 patients with left ventricular ejection fraction (EF) < or =35% and aortic stenosis with trans...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.101.16.1940
更新日期:2000-04-25 00:00:00
abstract:BACKGROUND:Prospective data relating previous exposure to cytomegalovirus (CMV) to the risk of cardiac mortality are controversial. We investigated the effect of previous exposure to CMV infection on the risk of future cardiac disease-related death in relation to an underlying inflammatory response. METHODS AND RESULT...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.103.24.2915
更新日期:2001-06-19 00:00:00
abstract:BACKGROUND:The pathogenesis of sodium and water accumulation in chronic constrictive pericarditis is not well understood and may differ from that in patients with chronic congestive heart failure due to myocardial disease. This study was undertaken to investigate some of the mechanisms. METHODS AND RESULTS:Using stand...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.83.6.1880
更新日期:1991-06-01 00:00:00
abstract:BACKGROUND:ST-segment depression during exercise testing is frequently observed in the absence of restenosis after coronary angioplasty. METHODS AND RESULTS:We studied the determinants of this phenomenon in 70 consecutive patients with unstable angina related to a single left anterior descending coronary artery lesion...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.90.1.148
更新日期:1994-07-01 00:00:00
abstract::To determine whether a ventricular (LV) end-systolic dimension (ESD) greater than or equal to 55 mm and LV left fractional shortening less than 25% are risk factors for aortic valve replacement (AVR) in patients with aortic insufficiency, we analyzed the clinical course and M-mode echocardiograms in 47 consecutive pat...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.67.1.216
更新日期:1983-01-01 00:00:00
abstract::Postoperative fever and pericardial-pleural reaction, designated postpericardiotomy syndrome (PPS), is a common complication of cardiac surgery involving entry into the pericardium. To determine whether the etiology of PPS is viral or immunologic, we undertook a prospective, triple-blind study of consecutive long-term...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.62.6.1151
更新日期:1980-12-01 00:00:00