Abstract：
:Previous studies have shown that, in unstimulated mammary epithelial cells from virgin mice, prolactin receptors are retained intracellularly because of their incomplete N-glycosylation. Activation of the nitric oxide/cGMP pathway stimulates Nacetylglucosamine (NAG) transferase I activity, completion of terminal glycosylation, and redistribution of the receptors to the cell surface. In this study, it was shown that nitric oxide could stimulate the phosphorylation of NAG transferase I in intact cells and that the cGMP-dependent protein kinase (PKG) could directly phosphorylate the purified enzyme. Furthermore, this modification was associated with enhanced enzymatic activity. Conversely, this stimulation of activity was blocked in intact cells by coincubation with a PKG inhibitor and reversed in the immunoprecipitated enzyme by alkaline phosphatase treatment. Kinetic analysis revealed that this effect on enzyme activity was due to an increase in V(max) without any change in K(m). Therefore, it appears that the nitric oxide/cGMP pathway activates NAG transferase I via direct phosphorylation by PKG.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Bolander FF Jrdoi
10.1677/jme.0.0240377subject
Has Abstractpub_date
2000-06-01 00:00:00pages
377-82issue
3eissn
0952-5041issn
1479-6813pii
JME00879journal_volume
24pub_type
杂志文章abstract：:An isolated in-vitro perifused interrenal gland preparation from the dogfish Scyliorhinus canicula was used to study production of quantitatively the major corticosteroid 1 alpha-hydroxycorticosterone (1 alpha-OH-B), measured by radioimmunoassay. Basal secretory rates were 877.1 +/- 145 (S.E.M.) fmol/mg per 15 min (n ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0050055
更新日期：1990-08-01 00:00:00
abstract：:Receptive endometrium is a prerequisite for successful embryo implantation, and it follows that poor endometrial receptivity is a leading cause of implantation failure. miRNAs play important roles as epigenetic regulators of endometrial receptivity and embryo implantation through post-transcriptional modifications. Ho...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-19-0184
更新日期：2020-01-01 00:00:00
abstract：:The cloning and characterization of the mouse TRH receptor (TRH-R) gene revealed an untranslated exon (exon 1), a single intron and an upstream dinucleotide repeat sequence (d(TG)16.d(AG)21) in the 5' untranslated region (UTR). The coding region was contained almost entirely on a second exon (exon 2), with the final a...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0110141
更新日期：1993-10-01 00:00:00
abstract：:A full-length transcript encoding a functional lamprey glycoprotein hormone receptor I (lGpH-R I, GenBank AY750688) was cloned from the testes of the sea lamprey, Petromyzon marinus, using the GpH-R protein fingerprint GLYCHORMONER from the PRINTS database. The present study is the first to identify a GpH-R transcript...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.02064
更新日期：2006-08-01 00:00:00
abstract：:The interaction between human papillomaviruses (HPV) and the apolipoprotein-B mRNA-editing catalytic polypeptide-like (APOBEC)3 (A3) genes has garnered increasing attention in recent years, with considerable efforts focused on understanding their apparent roles in both viral editing and in HPV-driven carcinogenesis. H...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-19-0011
更新日期：2019-05-01 00:00:00
abstract：:Reproductive function in mammals is energetically costly and therefore tightly regulated by nutritional status. To enable this integration of metabolic and reproductive function, information regarding peripheral nutritional status must be relayed centrally to the gonadotropin-releasing hormone (GNRH) neurons that driv...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-16-0212
更新日期：2017-02-01 00:00:00
abstract：:Obesity is likely to be a multifactorial disease with an important genetic component. Animal models of genetic and experimentally induced obesity suggest that glucocorticoid receptor (GR) activity plays a role in the aetiology and maintenance of the obese state. Glucocorticoid activity appears to be essential for the ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0090295
更新日期：1992-12-01 00:00:00
abstract：:Medullary thyroid carcinoma (MTC) is a rare tumour arising from neural crest-derived parafollicular C-cells. Metastatic MTC patients are incurable because the cancer does not respond to radiotherapy or chemotherapy. The REarranged during Transfection (RET) proto-oncogene plays a key role in the development of MTC. How...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/JME-09-0024
更新日期：2009-10-01 00:00:00
abstract：:In contrast to the classical model describing the synthesis of androgens and estrogens as restricted to somatic cells, a previous study demonstrated that Xenopus laevis oocytes participate in androgen synthesis. The objective of our study was to determine whether Xenopus oocytes are also involved in estrogen synthesis...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-11-0080
更新日期：2011-09-07 00:00:00
abstract：:Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Despite its essential roles in male reproductive and bone health, very little is known regarding its transcriptional regulation in Leydig cells. To date, few transcription factors h...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-15-0139
更新日期：2016-04-01 00:00:00
abstract：:Excess maternal glucocorticoids reduce placental amino acid transport and fetal growth, but whether these effects are mediated directly on the syncytiotrophoblast remains unknown. We hypothesised that glucocorticoids inhibit mechanistic target of rapamycin (mTOR) signaling and insulin-stimulated System A amino acid tr...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-19-0183
更新日期：2019-11-01 00:00:00
abstract：:In the common brushtail possum (Trichosurus vulpecula) lactation lasts for 200 days and consists of two distinct phases. Milk composition changes dramatically between phase 2 and 3, which correspond to early and late lactation respectively (phase 1 corresponds to pregnancy). RNA expression patterns have been establish...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0200037
更新日期：1998-02-01 00:00:00
abstract：:The major mission of the ovarian follicle is the timely production of the mature fertilizable oocyte, and this is achieved by gonadotropin-regulated, gap junction-mediated cell-cell communication between the oocyte and surrounding nurturing granulosa cells. We have demonstrated that FSH and transforming growth factor ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-15-0076
更新日期：2015-12-01 00:00:00
abstract：:Murine double minute clone 2 (MDM2) is a multifunctional protein, which modulates nuclear receptor-mediated transactivation. In this study, we show that MDM2 significantly enhanced estrogen receptor α (ERα) and ERα/specificity protein-mediated transactivation in MCF-7 and ZR-75 breast cancer cells. This was demonstrat...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-10-0110
更新日期：2011-02-15 00:00:00
abstract：:Urocortin (Ucn), a corticotropin-releasing factor (CRF)-related neuropeptide binding both CRF type 1 receptor (CRFR1) and CRFR2, has recently been found in prostate cancer. However, no report has yet been known to elucidate the roles of Ucn in prostate cancer via the two receptors. In this study, the expression of bot...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-11-0048
更新日期：2011-09-07 00:00:00
abstract：:The chemokine Stromal-derived factor-1alpha (SDF-1alpha) interacts with seven transmembrane (TM) G-protein-coupled receptor (GPR), CXCR4. SDF-1alpha is linked to inflammation, chemoattraction, cancer metastasis, and hematopoiesis. Tachykinin (Tac1) peptides bind seven transmembrane (TM), GPR and are involved in tumor ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-07-0111
更新日期：2008-03-01 00:00:00
abstract：:High energy intake and, specifically, high dietary fat intake challenge the mammalian metabolism and correlate with many metabolic disorders such as obesity and diabetes. However, dietary restriction (DR) is known to prevent the development of metabolic disorders. The current western diets are highly enriched in fat, ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:The crystal structures of the leucine-rich repeat domain (LRD) of the FSH receptor (FSHR) in complex with FSH and the TSH receptor (TSHR) LRD in complex with the thyroid-stimulating autoantibody (M22) provide opportunities to assess the molecular basis of the specificity of glycoprotein hormone-receptor binding. A com...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0040
更新日期：2008-09-01 00:00:00
abstract：:Angiotensin II (AII) receptors were identified in rat tissue membranes by specific binding of 125I-labelled AII. Using an isoelectric focusing technique, two forms of the high-affinity AII receptor were identified in rat adrenal zona glomerulosa and liver membranes. These migrated to isoelectric points (pI) 6.8 and 6....
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0070021
更新日期：1991-08-01 00:00:00
abstract：:Two marsupial casein genes have been isolated from a tammar wallaby (Macropus eugenii) mammary gland cDNA library. Comparisons of the tammar alpha- and beta-casein genes with their eutherian homologous reveal extensive divergence at the levels of nucleotide and amino acid sequences. Regions of similarity between the t...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0080013
更新日期：1992-02-01 00:00:00
abstract：:Decreased suppressors of cytokine signaling (SOCS) activity in human gestational tissues may play a part in the onset/progression of term labor. Since SOCS proteins negatively regulate cytokine-mediated inflammatory processes, we hypothesized that SOCS proteins are elevated in gestational tissues from spontaneous pret...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01767
更新日期：2005-08-01 00:00:00
abstract：:During the process of insulitis in the pathogenesis of type I (insulin-dependent) diabetes mellitus, proinflammatory cytokines induce expression of the death receptor Fas on the surface of pancreatic beta-cells and thereby contribute to the enhanced susceptibility of beta-cells for apoptosis. The aim of this study was...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0300163
更新日期：2003-04-01 00:00:00
abstract：:Parathyroid hormone (PTH), a powerful bone-resorbing agent, is capable of stimulating interstitial collagenase (MMP-13) mRNA production in osteoblastic cells. In this study, a PEA3 consensus binding sequence (-80; AGGAAGT) in addition to a 'TRE-like' sequence (-89; CGACTCA) in the 5' upstream regulatory region of the ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0250073
更新日期：2000-08-01 00:00:00
abstract：:Testicular Leydig cells (LC) are modulated by several pathways, one of them being the histaminergic system. Heme oxygenase-1 (HO-1), whose upregulation comprises the primary response to oxidative noxae, has a central homeostatic role and might dysregulate LC functions when induced. In this report, we aimed to determin...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-19-0063
更新日期：2019-10-01 00:00:00
abstract：:This study investigates the effectiveness and mechanisms of a serum- and glucocorticoid-inducible kinase 1 (SGK1) inhibitor in counteracting hyperglycemia. In an in vivo experiment, we demonstrated that after an 8-week treatment with an SGK1 inhibitor, the fasting blood glucose and HbA1c level significantly decreased ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-15-0285
更新日期：2016-05-01 00:00:00
abstract：:The constitutive expression of 25-hydroxyvitamin D3-24-hydroxylase (25-(OH)D3-24-hydroxylase) activity has been studied in an adherent variant (Ad-HL60) of the human promyelomonocytic leukaemia cell line HL60. The Ad-HL60 cells have a more differentiated phenotype than the non-adherent cells from which they were deriv...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0070185
更新日期：1991-12-01 00:00:00
abstract：:Seven bovine follistatin (FS) cDNA clones were isolated from a bovine ovarian follicle cDNA library. The predicted amino acid sequences revealed that six of the cDNA clones represented an FS precursor of 344 amino acids which corresponded to a mature FS of 315 amino acids (FS 315), with one cDNA clone containing the e...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0130321
更新日期：1994-12-01 00:00:00
abstract：:Tumor malignancy is associated with the epithelial-mesenchymal transition (EMT) process and resistance to chemotherapy. However, little is known about the relationship between the EMT and the multidrug-resistance gene in thyroid tumor progression. We investigated whether the expression of the ABCG2/BCRP gene is associ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-14-0051
更新日期：2014-04-28 00:00:00
abstract：:DNA encoding the N-terminal 415 residues of the human thyrotrophin receptor (predicted to code for the large extracellular region) was introduced into Chinese hamster ovary (CHO) cells using the glutamine synthetase/cytomegalovirus amplifiable expression system, and into E. coli using the pGEX-3X expression vector. Su...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0090227
更新日期：1992-12-01 00:00:00
abstract：:Unliganded thyroid hormone receptors (apoTRs) repress transcription of hormone-activated genes by recruiting corepressors to the promoters. In contrast, on promoters containing so-called negative thyroid hormone response elements (nTREs), apoTRs activate transcription. A number of different molecular mechanisms have b...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01874
更新日期：2006-06-01 00:00:00
