Abstract:
:It has been postulated that patients with ulcerative colitis (UC) have altered reactivity of gut-associated lymphoid tissue. In such cases there is intense infiltration of the mucosa with immune competent cells and associated tissue damage. We have shown previously that the dietary supplementation with the n-3 polyunsaturated fatty acids (n-3 PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) results in significant systemic immune suppression. The aim of this study, therefore, was to evaluate the in situ effect of n-3 PUFAs on distal proctocolitis. Each patient received either fish oil extract (EPA 3.2 g, DHA 2.4 g) (n = 9) or sunflower oil (n = 9) daily in a double blind manner for six months. Monthly assessment included: (1) disease activity using clinical, sigmoidoscopic, and histological scores and (2) immunohistochemical analysis (immunoglobulins, CD profiles) of rectal biopsy specimens (before and after six months supplementation) using monoclonal antibodies and quantitative computer-assisted video image analysis. Prior to receiving supplementation, patients with proctocolitis (n = 18) showed significantly higher numbers of cells expressing CD3 (pan T cells) and HLA-DR and IgM containing cells compared with non-colitic controls (n = 8). Six months supplementation with n-3 PUFAs resulted in significant reduction in the number of cells expressing CD3 and HLA and the percentage of cells containing IgM. There was no significant change in the CD20 nor the percentage of IgG or IgA containing cells in either group of patients with procto-colitis. In patients receiving n-3 PUFA supplementation, there was improvement in the disease activity and histological scores, compared with pretreatment evaluation. This study has demonstrated both evidence of suppression of in situ immune reactivity and concurrent reduction in disease activity in patients with proctocolitis receiving n-3 PUFA supplementation. This may have important implication for therapy in patients with ulcerative colitis.
journal_name
J Clin Immunoljournal_title
Journal of clinical immunologyauthors
Almallah YZ,Ewen SW,El-Tahir A,Mowat NA,Brunt PW,Sinclair TS,Heys SD,Eremin Odoi
10.1023/a:1006698728816subject
Has Abstractpub_date
2000-01-01 00:00:00pages
68-76issue
1eissn
0271-9142issn
1573-2592journal_volume
20pub_type
临床试验,杂志文章,随机对照试验abstract::The sensitivity of tumor cells to lysis by natural killer (NK) and interleukin-2 (IL-2)-activated killer (LAK) cells was studied in three ovarian carcinoma cell lines (2780.9S, SKOV-3, and CHOAUXB1), four multidrug-resistant (MDR) variants, and a melphalan-resistant line. The antitumor activity of LAK cells was evalua...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF01541671
更新日期:1996-11-01 00:00:00
abstract::IgD production by short-term human peripheral blood mononuclear cell (PBM) cultures was studied to establish the in vitro correlates of low serum IgD expression. Cells of persons with less than 3 micrograms/ml IgD in the serum, referred to as the low-serum IgD phenotype (LISP), were analyzed. Advantage was taken of re...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00916005
更新日期:1987-03-01 00:00:00
abstract::The origin of natural antibodies has long been a subject of controversy. Polyreactive natural antibodies recognize multiple ligands and are thought to arise from B1 B cells. Natural antibodies against carbohydrate antigens such as Gal alpha 1-3Gal or against blood groups A and B are thought to be "elicited" by gut bac...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1023/a:1027378716015
更新日期:1997-07-01 00:00:00
abstract::Live-attenuated vaccines (LAVs) can protect humans against 12 viral and three bacterial diseases. By definition, any clinical infection caused by a LAV that is sufficiently severe to require medical intervention attests to an inherited or acquired immunodeficiency that must be diagnosed or identified. Self-healing inf...
journal_title:Journal of clinical immunology
pub_type: 杂志文章,评审
doi:10.1007/s10875-019-00642-3
更新日期:2019-05-01 00:00:00
abstract::Treatment decisions made in clinical practice, based on current guidelines, often conflict with decisions by third-party payors that restrict the ability of patients with primary immunodeficiency disease (PI) to adhere to appropriate treatment. This is seen by many physicians as potentially placing the health of patie...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-012-9723-y
更新日期:2012-09-01 00:00:00
abstract:INTRODUCTION:Systemic lupus erythematosus (SLE) is a systemic autoimmune disease associated with aberrant activation of T and B lymphocytes. Abnormal activation of intracellular signaling molecules in lymphocytes by inflammatory cytokines can instigate the inflammation in SLE. MATERIALS AND METHODS:The activation of e...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-009-9318-4
更新日期:2009-11-01 00:00:00
abstract::Using an enzyme-linked immunoassay we tested the sera of 71 patients with digestive system cancer, 35 patients with various nonmalignant disorders, and 28 normal individuals for anti-CEA activity. Antibodies were found in the sera of 51% of the patients. Most of the patients positive for the antibodies (70%) had no ev...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF01540984
更新日期:1994-09-01 00:00:00
abstract:PURPOSE:T-helper (Th) cells abnormalities are considered to be associated with the pathogenesis of Systemic lupus erythematosus (SLE). Recently, The Th22 cells have been identified and implicated in the pathogenesis of autoimmune diseases such as Rheumatoid arthritis (RA), although therir role in Systemic lupus erythem...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-013-9878-1
更新日期:2013-05-01 00:00:00
abstract::Cytokines are powerful mediator and communication molecules capable of regulating a wide spectrum of biologic functions, including immune responses. Although the role of cytokines in the pathogenesis of human disease is not yet understood, assays for cytokines have become a common feature in research and clinical labo...
journal_title:Journal of clinical immunology
pub_type: 杂志文章,评审
doi:10.1007/BF01546317
更新日期:1994-11-01 00:00:00
abstract::Augmentation of the cytolytic activity of human natural killer cells and of antibody-dependent cell-mediated cytotoxicity has been attributed to human interferons. With the purification to homogeneity of human leukocyte interferon, it became possible to test directly whether pure interferon could increase the activity...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00922756
更新日期:1981-07-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1007/s10875-007-9096-9
更新日期:2007-09-01 00:00:00
abstract:BACKGROUND:Nonalcoholic steatohepatitis (NASH) is a chronic inflammatory liver disease influenced by risk factors for the metabolic syndrome. In adult patients, NASH is associated with an altered phenotype and functionality of peripheral immune cells, the recruitment of leukocytes and intrahepatic activation, and an ex...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-011-9635-2
更新日期:2012-06-01 00:00:00
abstract::Among a population of uniformly differentiated TH(2) cells, only a portion express IL-4 upon stimulation and those that do often express the product of only a single allele. We review the evidence for the basis of IL-4 monoallelism and argue that it depends upon probabilistic expression of the Il4 gene. Further, we ar...
journal_title:Journal of clinical immunology
pub_type: 杂志文章,评审
doi:10.1007/s10875-005-8218-5
更新日期:2005-11-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00919268
更新日期:1993-03-01 00:00:00
abstract::Data collection for the national registry for patients with primary immunodeficiency disorders in the Republic of Ireland commenced in 1996. One hundred and fifteen cases of primary immunodeficiency diseases were registered between December 1996 and February 2003. The most frequent primary immunodeficiency disorders w...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-005-0360-9
更新日期:2005-01-01 00:00:00
abstract::Persistent, generalized lymphadenopathy (PGL) is a recognized component of human immunodeficiency virus (HIV) infection. We conducted longitudinal studies of B and T cell function in seven homosexual men with HIV infection and PGL. All seven had abnormal antibody-mediated immunity as studied by sequential assessment o...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00915157
更新日期:1988-01-01 00:00:00
abstract::The basis of the immunological unresponsiveness seen in leprosy patients is unknown. Untreated lepromatous leprosy patients display an unspecific cellular anergy which disappears with treatment, leaving an anergy specific for Mycobacterium leprae. These patients suffer from a complication, erythema nodosum leprosum, c...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00916889
更新日期:1982-04-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-006-9053-z
更新日期:2007-01-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章,评审
doi:10.1023/b:joci.0000019809.55787.ec
更新日期:2004-03-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1023/a:1027320425291
更新日期:1997-09-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00916951
更新日期:1988-11-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-006-9052-0
更新日期:2007-01-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-008-9203-6
更新日期:2008-09-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/s10875-008-9268-2
更新日期:2009-05-01 00:00:00
abstract::Testing of cytotoxic function using a panel of natural killer (NK)-sensitive target cells, including a unique herpes simplex virus-infected Raji-cell target, was performed in conjunction with phenotypic cell analysis by dual-color flow cytometry to characterize the NK system. Subjects included in the study were at ris...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00915420
更新日期:1987-01-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00918662
更新日期:1989-07-01 00:00:00
abstract::IL-15 is essential for the development and differentiation of NK cells. It selectively induces proliferation of CD8+ memory T lymphocytes. Despite its importance in both innate and adaptive immune responses, little is known about its production in HIV-infected persons. We report here that IL-15 levels are significantl...
journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1023/a:1022568626500
更新日期:2003-03-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
doi:10.1007/BF00920633
更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Journal of clinical immunology
pub_type: 杂志文章
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