Propofol anaesthesia in mice is potentiated by muscimol and reversed by bicuculline.

Abstract:

:We have examined the role of gamma-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED50 value of 140 (95% confidence limits 123-160) mg kg-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED50 for propofol decreased significantly to 66 (58-75) mg kg-1 in the presence of the GABAA receptor agonist muscimol 1 mg kg-1 i.p. (n = 40) (P < 0.05). In contrast, the ED50 increased significantly to 240 (211-274) mg kg-1 in the presence of the antagonist bicuculline 5 mg kg-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part by GABA neurons.

journal_name

Br J Anaesth

authors

Irifune M,Sugimura M,Takarada T,Maeoka K,Shimizu Y,Dohi T,Nishikawa T,Kawahara M

doi

10.1093/bja/83.4.665

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

665-7

issue

4

eissn

0007-0912

issn

1471-6771

pii

S0007-0912(17)38310-1

journal_volume

83

pub_type

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