Abstract：
BACKGROUND:Bacillus Calmette-Guérin (BCG) vaccine is a non-specific immunostimulant which has been used clinically in the treatment of melanoma. In this communication, the antimelanoma activity of BCG was related to its fibronectin-binding properties and mimicked using gelatin nanoparticles. MATERIALS AND METHODS:The fibronectin-binding properties of aqueous gelatin solutions, gelatin nanoparticles, BCG vaccine, and PS1 (a glucan extracted from Tice BCG) were compared by an enzyme-linked immunosorbent assay and their ability to suppress murine B16-F0 melanoma in vivo investigated. RESULTS:Aqueous gelatin solutions, gelatin nanoparticles and BCG all bound to fibronectin in vitro. The immunostimulant PS1 did not. In vivo, BCG and gelatin nanoparticles suppressed melanoma growth while PS1 and aqueous gelatin solutions had no effect. CONCLUSIONS:The antimelanoma activity of BCG is not due to the associated immunostimulatory glucan but can be correlated to its fibronectin-binding properties. Since solutions of gelatin have no effect whereas nanoparticles produce total suppression, this suggests a relationship between the volume of the fibronectin-binding entities and their antitumour activity. Thus, gelatin nanoparticles may represent an attractive alternative to the use of BCG vaccine in melanoma treatment.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Farrugia CA,Groves MJsubject
Has Abstractpub_date
1999-03-01 00:00:00pages
1027-31issue
2Aeissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract：AIM:To characterize neurotoxicity induced by oxaliplatin, bortezomib, and epothilone-B as well as protection against their neurotoxicity using an in vitro model. MATERIALS AND METHODS:Neurotoxicity was evaluated using the neurite outgrowth method in PC12 rat pheochromo-cytoma cells differentiated towards a mature neur...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The effects of the antineoplastic compound, vinorelbine, on the permeability of the blood-brain barrier (BBB) to the complex Evans blue/albumin were studied. Vinorelbine, at a dose range from 5 to 10 mg/kg, was infused (4 ml/kg over 2 min) into the left carotid artery of anesthetised male Sprague-Dawley rats. BBB disr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The effects of dibutyryl cyclic AMP (DBcAMP) on tissue inhibitor metalloproteinase (TIMP) expression were studied in the human hepatoma cell line PLC/PRF/5 with relation to the invasive activity of the cells. Messenger RNA expression levels of metalloproteinases (MMP)-2 and 9, and TIMP-1, 2 and 3 in the cells were det...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND:Extracts from Viscum album L. are widely used as an adjuvant in complementary cancer therapy. While the mechanisms of mistletoe lectin (ML) cytotoxicity are well described, the viscotoxin (VT) effects are unclear at present. Thus, we treated human lymphocytes with VT and measured cell death-associated change...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：BACKGROUND:A pitfall of immunotherapy with dendritic cells (DCs) for patients with malignant tumor is that certain patients have already been treated with Dexamethasone (DEX) prior to vaccination. Therefore, it is important to investigate whether DEX has negative effects on induction of antitumor immune response in den...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:In germ cell testicular tumors (GCTT) mdm-2 gene was analyzed for amplification and transcripts but not for protein. The purpose of this study is to determine whether mdm-2 protein level is aberrant in GCTT and if so, what is the relationship between mdm-2 overexpression and other disease parameters includin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Caspase-3 is a key executioner cysteine protease involved in programmed cell death or apoptosis. A ribozyme to human caspase-3 was designed, tested by in vitro cleavage, and transfected into a drug-resistant variant (DLKP-A5F) of a human lung carcinoma cell line (DLKP). By both stable and transient transfection, this ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Human immortal cells maintain their telomeres either by telomerase or by alternative lengthening of telomeres (ALT) that is based on homologous telomeric recombination. Previous studies showed that the ALT mechanism is activated in non-ALT cells when heterochromatic features are reduced. In this study, we examined the...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:P-glycoprotein has been identified in mast cells stabilized in culture as well as in rat peritoneal mast cells, and is primarily concentrated on the granular membrane. This study aimed to define the role of this protein in the transport and accumulation of doxorubicin in mast cell granules and in its histamine releasi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:To determine whether an imbalanced interaction between proapototic and antiapoptotic signals may account for the loss of the normal cell growth control in benign prostatic hyperplasia (BPH), the expression of some apoptosis-regulating genes (bcl-2, bax, c-myc, fas) was investigated. PATIENTS AND METHODS:BPH...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Seventy natural and synthetic compounds were tested for potential to inhibit mutation induced by 7,12-dimethylbenz(a)anthracene (DMBA) in Salmonella typhimurium strain TM677. Results were compared with their ability to inhibit DMBA-induced preneoplastic lesions in a mouse mammary gland organ culture system. The respon...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:The usefulness of lymphocyte subset change as an indicator for predicting survival time and the effectiveness of combined treatment with an immunopotentiator, lentinan, and carboplatin (CBDCA) were investigated. Of 13 patients with advanced unresectable cancer entered in this study, 9 were administered 2 mg/body lenti...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Reduction of nitroblue tetrazolium by blood monocytes was significantly increased following post-operative radiation therapy for breast cancer. Attachment and ingestion of yeast particles by monocytes was not affected. Exposure of purified monocytes in vitro to 13 Gy did not significantly affect NBT reduction of yeas...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：BACKGROUND:Our aim was to analyze the impact of intraperitoneal chemotherapy (IPC), administered with direct peritoneal puncture, on the survival of patients with pretreated ovarian cancer in a real-life setting. PATIENTS AND METHODS:This was a retrospective study comparing patients with advanced ovarian cancer treate...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11256
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:The diversity of malignant salivary gland tumors challenges the study of survival rates. The current study evaluated patient survival rates using Kaplan-Meier analysis and examined the relative effects of histology, grade and stage on survival. MATERIALS AND METHODS:Using the Kaplan-Meier model, cancer-spec...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13158
更新日期：2019-02-01 00:00:00
abstract：:Germ line point mutations in the RET proto-oncogene have been implicated in four inherited disorders: multiple endocrine neoplasia 2A (MEN 2A) and 2B (MEN 2B); familial medullary thyroid carcinoma (FMTC); and Hirschprung's disease, a congenital lack of enteric plexus neurons. Oncogenically activated RET has also been ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Radiation therapy is essential for local tumor control for many types of cancer histologies. Technological advancements in recent years have allowed for precise irradiation of target tissues while minimizing the dose to non-target tissues. To enhance radiation damage to cancer cells and further limit the radiation eff...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2015-05-01 00:00:00
abstract：BACKGROUND/AIM:Several studies have revealed an association between single nucleotide polymorphisms (SNPs) in the VDR gene and prostate cancer (PCa) risk in European and Asian populations. To investigate whether VDR SNPs are associated with PCa risk in African-American (AA) men, nine VDR SNPs were analyzed in a case-co...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：BACKGROUND/AIM:Chemotherapy-associated toxicity is one of the limiting factors regarding treatment efficacy, patient outcome and quality of life in this collective. Underweight or obese patients represent a major group in which the therapy seems to be more challenging. The aim of this analysis was to evaluate the impac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12927
更新日期：2018-10-01 00:00:00
abstract：:We have examined several aspects of protein tyrosine kinase (PTK)-activity in the cytosolic and membrane fractions of both normal and malignant lymphoid cells. The expression of the PTK-encoding oncogenes fes, abl and src, was investigated with the use of antibodies generated to their respective gene products. These a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：AIM:Reduced right ventricular (RV) systolic function correlates with poor prognosis in several heart diseases. The aim of this prospective single-Center study was to investigate whether conformal three-dimensional (3D) breast cancer radiotherapy impairs RV function. PATIENTS AND METHODS:Forty-nine patients with early-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
abstract：:The aim of this study was to evaluate the usefulness of image-guided robotic stereotactic radiosurgery for the local control of unresectable liver metastases from colorectal and non-colorectal cancer. Twenty-seven consecutive patients (median age 62 years, range 47-80 years) with liver metastases considered unsuitable...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：BACKGROUND/AIM:Previous genetic and morphologic characterisation of mucinous tubular and spindle cell carcinoma (MTSCC) have yielded controversial results. The aim of this study was to explain the phenotypic heterogeneity of MTSCC diagnosed by genetic means. MATERIALS AND METHODS:We analyzed 7 MTSCC by array CGH and m...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11432
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND/AIM:Combining an anticancer agent fenretinide (HPR) or C6-pyridinium ceramide (LCL29) with Foscan-mediated photodynamic therapy (FoscanPDT) is expected to augment anticancer benefits of each substance. We showed that treatment with FoscanPDT+HPR enhanced accumulation of C16-dihydroceramide, and that fumonisi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11337
更新日期：2017-02-01 00:00:00
abstract：:When mouse resident peritoneal macrophages were cultured with PSK (Krestin), a protein-bound polysaccharide extracted from Coriolus versicolor, they became enlarged and elongated and expressed higher NBT-reducing activity. PSK stimulated the production of differentiation-inducing factor and cytotoxic factor by the mou...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:Flow cytometric DNA analysis was performed in 67 tubular, tubulovilous and villous rectal adenomas with different degree of dysplasia. One of the adenomas contained a focus of early invasive carcinoma. All but one of the 67 adenomas had near diploid DNA histograms; the exception was a tubular adenoma with severe dyspl...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：BACKGROUND:Late developing breast cancer metastases are common and lethal despite treatment with adjuvant chemotherapy at the time of primary tumor excision. Stimulation of an antitumor immune response is an alternative strategy for preventing this devastating development. MATERIALS AND METHODS:A mouse model of the hu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13029
更新日期：2018-12-01 00:00:00
abstract：:Small cell lung cancer (SCLC) is highly sensitive both to radiotherapy and chemotherapy. Given its high chemo sensitivity, even two decades ago, SCLC was one of the first malignancies deemed suitable for maximising the dose and dose intensity with the support of autologous bone marrow (ABMT). On the whole, results wer...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2002-11-01 00:00:00
abstract：:Cytotoxicity of L-canavanive, a structural analogue of L-arginine, was tested by means of (1) inhibition of DNA synthesis in Balb/c-3T3 cell line and (2) inhibition of mitosis in peripheral blood lymphocytes in culture. The cytostatic nature of inhibition caused by L-canavanine was indicated by the complete recovery o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:The aim of this study was to investigate the diagnostic value of FDG PET in the follow-up for breast cancer in disease-free patients and patients suspected of having recurrent or metastatic disease. As a single imaging tool, PET can be compared with the conventional diagnostic means used for different examin...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-03-01 00:00:00
