Abstract：
BACKGROUND/AIMS:The ability to transfer foreign genes into the biliary tract would be useful for the treatment of biliary tract diseases, including cancer, cystic fibrosis and other genetic diseases. To introduce a foreign gene precisely into the rat biliary epithelial cells, we developed a new technique, inserting a polyethylene catheter into the common bile duct through the papilla of Vater by use of a fusigenic cationic liposome with hemagglutinating virus of Japan (HVJ-cationic liposome). METHODS:Transfection efficiency was estimated with the use of FITC-oligonucleotides (FITC-ODNs) and cDNA of beta-galactosidase (pCAG-lacZ). RESULTS:FITC-ODNs encapsulated in HVJ-cationic liposome were effectively transfected into cell nuclei of human cholangiocellular carcinoma in vitro after a 30-min incubation as compared with the simple application of naked FITC-ODNs. After in vivo injection of FITC-ODNs using the HVJ-cationic liposome method through the papilla of Vater, fluorescence accumulation was observed only in the epithelial cells of the biliary tract, but not in the parenchymal cells of the liver. Beta-galactosidase expression was observed in the biliary epithelial cells 3 days after the transfection of pCAG-lacZ and was also detected at 14 days, but not at 28 days, without obvious cytotoxicity. CONCLUSIONS:HVJ-cationic liposome-mediated gene transfer to the biliary tract via the papilla of Vater is a minimally-invasive and an effective gene-delivery method for site-specific targeting to the epithelial cells of the biliary tract, which could be applied to the treatment of human biliary tract diseases.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Uehara T,Honda K,Hatano E,Terao R,Iimuro Y,Yamamoto N,Yamamoto M,Kaneda Y,Yamaoka Ydoi
10.1016/s0168-8278(99)80137-8subject
Has Abstractpub_date
1999-05-01 00:00:00pages
836-42issue
5eissn
0168-8278issn
1600-0641pii
S0168-8278(99)80137-8journal_volume
30pub_type
杂志文章abstract：:Extracellular vesicles are membrane-bound vesicles containing proteins, lipids, RNAs and microRNAs. They can originate from both healthy and stressed cells, and provide a snapshot of the cell of origin in physiological and pathological circumstances. Various processes that may give rise to the release of extracellular...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2020.07.014
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND/AIMS:Excessive formation of nitric oxide may mediate the generalized vasorelaxation and hyporesponsiveness to vasoconstrictors observed in portal hypertensive states. Endotoxin, released from the bowel and detoxified by the liver, could stimulate inducible nitric oxide synthase directly or indirectly via the...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80266-9
更新日期：1999-06-01 00:00:00
abstract：BACKGROUND & AIMS:Sustained viral response (SVR) after therapy with interferon-ribavirin (IF-RB) reduces liver-related (LR) complications and mortality in HIV/HCV-co-infected patients. Here, we assess the impact of end-of-treatment response with subsequent relapse (REL) on LR events (LR death, liver decompensation, hep...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2013.01.042
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND/AIMS:Pregnant women with acute viral hepatitis (VH) and those with pregnancy associated acute liver disease (PAALD) including acute fatty liver disease of pregnancy, hemolysis elevated liver enzyme and low platelet syndrome present with similar clinical features and liver tests abnormalities. Accurate differ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2008.07.030
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND/AIMS:To analyze the kinetics of the hepatitis C virus and the patterns of resistance to interferon alpha, we assessed HCV RNA levels early during retreatment with high-dose interferon in patients who did not respond to standard treatment. METHODS:Eleven non-responders to previous therapy with 3-6 MU interfe...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/s0168-8278(98)80343-7
更新日期：1998-06-01 00:00:00
abstract：BACKGROUND/AIMS:This study aimed to determine whether there is an increased infectious risk following liver biopsy in liver transplant patients with choledochojejunostomy. METHODS:We evaluated the incidence of liver-biopsy-related sepsis in a consecutive series of 27 patients who underwent choledochojejunostomy, eithe...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(96)80012-2
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND/AIM:Chronic rejection is an important cause of graft loss following liver transplantation. A number of risk factors for chronic rejection have been identified previously, albeit inconsistently. These include cytomegalovirus infection detected by a number of different techniques, including immunohistochemical...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80294-3
更新日期：1999-11-01 00:00:00
abstract：:The natural history of HBV is known to be complicated by HIV-co-infection. In contrast, the effect of HBV on the outcome of patients infected with HIV-1 is controversial. Some cohort studies from the pre-HAART era described a more rapid progression to AIDS in patients carrying antibodies to the core-antigen or having ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2005.11.007
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND/AIMS:Because dietary modifications have prolonged the life expectancy of patients with glycogen storage disease type Ia (GSD Ia), the incidence of hepatocellular adenoma (HCA) to carcinoma (HCC) transformation is increasing. The objective of this retrospective study is to assess the safety and effectiveness ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2007.05.012
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND & AIM:Virus-induced fulminant hepatitis is a major cause of acute liver failure. During acute viral hepatitis the impact of type I interferon (IFN-I) on myeloid cells, including liver-resident Kupffer cells (KC), is only partially understood. Herein, we dissected the impact of locally induced IFN-I responses...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2017.11.029
更新日期：2018-04-01 00:00:00
abstract：:Activities of superoxide dismutase, catalase and glutathione peroxidase were measured in liver biopsy specimens from patients with various liver diseases, including six with chronic persistent hepatitis, nine with chronic active hepatitis, nine with non-alcoholic cirrhosis, eight with alcoholic cirrhosis and eight wit...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(90)90114-7
更新日期：1990-09-01 00:00:00
abstract：BACKGROUND & AIMS:Steatohepatitis (SH) is associated with mitochondrial dysfunction and excessive production of superoxide, which can then be converted into H(2)O(2) by SOD2. Since mitochondrial GSH (mGSH) plays a critical role in H(2)O(2) reduction, we explored the interplay between superoxide, H(2)O(2), and mGSH in n...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.05.024
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND & AIMS:Aceruloplasminemia is a rare autosomal recessive neurodegenerative disease associated with brain and liver iron accumulation which typically presents with movement disorders, retinal degeneration, and diabetes mellitus. Ceruloplasmin is a multi-copper ferroxidase that is secreted into plasma and facil...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.04.039
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND/AIMS:Evidence that the geno/subtype of hepatitis C virus (HCV) is predictive of the response to interferon-alpha therapy suggests that typing methods are clinically useful. In the present study, HCV isolates obtained from 74 patients with chronic hepatitis C were used to evaluate three genotyping and two ser...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80108-0
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND/AIMS:Progressive familial intrahepatic cholestasis type 2 (PFIC-2) patients have a defect in the hepatocanalicular bile salt secretion. The disease is caused by mutations in the bile salt export pump (BSEP). Ten different missense mutations have been described. In this study, we analysed the effect of the D4...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00483-5
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND/AIMS:Fulminant hepatic failure (FHF) can be dreadful. When coma sets in, brain edema develops taking FHF into a lethal course. Mechanisms of brain extravasation leading to brain edema remain incompletely understood. Matrix metalloproteinase (MMP)-9 is implicated in various brain injuries. We hypothesized tha...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.09.019
更新日期：2006-06-01 00:00:00
abstract：:Management of decompensated cirrhosis is currently geared towards the treatment of complications once they occur. To date there is no established disease-modifying therapy aimed at halting progression of the disease and preventing the development of complications in patients with decompensated cirrhosis. The design of...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2020.08.009
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND & AIMS:Non-alcoholic fatty liver disease (NAFLD) comprises a spectrum of stages from simple steatosis to non-alcoholic steatohepatitis (NASH). However, disease pathogenesis remains largely unknown. microRNA (miRNA or miR) expression has recently been reported to be altered in human NASH, and modulated by urs...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.08.008
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND & AIMS:The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is affected by epigenetic factors as well as by genetic variation. METHODS:We performed targeted-bisulfite sequencing to determine the levels of DNA methylation of 4 CpG islands (CpG99, CpG71, CpG26, and CpG101) in the regulatory regions o...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2015.02.049
更新日期：2015-08-01 00:00:00
abstract：:A quantitative immunohistochemical study of Kupffer cells in liver biopsies from alcoholics was carried out. Two markers were compared, lysozyme and an antimacrophage monoclonal antibody EMB/11. The results showed that there is no significant reduction in Kupffer cell numbers in acute and chronic alcoholic liver disea...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(89)90005-6
更新日期：1989-03-01 00:00:00
abstract：:A joint EASL/AASLD Monothematic Conference on 'Nuclear Receptors and Liver Disease' was held from February 27th to March 1st, 2009, in Vienna, Austria, to discuss the latest advances at the forefront of basic and clinical nuclear receptor research and its potential implications for liver diseases. This article reports...
journal_title：Journal of hepatology
pub_type：
doi：10.1016/j.jhep.2009.12.002
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND & AIMS:Tumor shrinkage has been considered a fundamental surrogate efficacy measure for new cancer treatments. However, in patients treated with sorafenib for advanced hepatocellular carcinoma (HCC), tumor shrinkage rarely accompanies increased survival, thereby questioning the prognostic value of imaging-ba...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.02.016
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND/AIMS:Interaction between CYP2E1, ethanol metabolites, and enhanced lipid peroxidation is linked to the pathogenesis of alcoholic liver disease. This study was conducted to compare the expression of various cytochrome enzymes and the appearance of aldehyde adducts in humans. METHODS:Acetaldehyde- and lipid p...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)80120-8
更新日期：2000-12-01 00:00:00
abstract：:The etiologic agent responsible for epidemics of enterically-transmitted non-A, non-B hepatitis has been molecularly characterized as the hepatitis E virus (HEV). The cloning of a portion of the Burma strain of HEV (HEV(B); 'Old World' strain) has been described together with the isolation of a contiguous overlapping ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(91)90050-l
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND/AIMS:Liver allograft is known to protect simultaneously transplanted organs from acute rejection. We have reported that only 6% of combined liver-kidney recipients, versus 32.5% of kidney recipients, develop kidney graft acute rejection. Release of soluble human leukocyte antigen (HLA) molecules by the liver...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00354-4
更新日期：2003-10-01 00:00:00
abstract：BACKGROUND/AIMS:The primary cause of Familial Amyloidotic Polyneuropathy is a variant transthyretin gene on chromosome 18. Progressive polyneuropathy followed by fatal cardiac and renal failure commonly manifest during middle age. Within 10 years after onset of clinical symptoms, affected individuals usually die due to...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80076-2
更新日期：1999-02-01 00:00:00
abstract：:As growth hormone possesses anabolic properties that are active on protein metabolism, and thus of potential benefit to patients with chronic liver disease, we determined the metabolic effects of recombinant human growth hormone on insulin-like growth factor-I (IGF-I) its specific binding proteins, and liver function....
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0168-8278(94)80228-9
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND & AIMS:Data on acute exacerbation and reactivation of chronic hepatitis C virus (HCV) infection following chemotherapy are very limited. We sought to characterize the episodes of acute exacerbation and viral reactivation of HCV infection in cancer patients. METHODS:The medical records of HCV-infected patien...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.07.031
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND/AIMS:Although 6-thioguanine (6-TG) has been suggested as an effective treatment option for patients with inflammatory bowel disease (IBD), the recent description of its hepatotoxicity has led to the recommendation not to consider this drug. We initiated a multicenter safety study in IBD-patients treated with...
journal_title：Journal of hepatology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jhep.2005.02.051
更新日期：2005-08-01 00:00:00
abstract：BACKGROUND & AIMS:Biliary tract cancers (BTCs) are clinically and pathologically heterogeneous and respond poorly to treatment. Genomic profiling can offer a clearer understanding of their carcinogenesis, classification and treatment strategy. We performed large-scale genome sequencing analyses on BTCs to investigate t...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2018.01.009
更新日期：2018-05-01 00:00:00
