An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies.

abstract：:We report the results of consecutive tests in nine BCR-ABL-positive ALL patients by one-step and two-step (nested primer) reverse transcriptase-polymerase chain reaction (RT-PCR). Six patients could be tested in complete haematological remission (CHR). One patient remained one-step positive; four patients became one-s...

journal_title：British journal of haematology

authors： Mitterbauer G,Födinger M,Scherrer R,Knöbl P,Jäger U,Laczika K,Schwarzinger I,Gaiger A,Geissler K,Greinix H

更新日期：1995-04-01 00:00:00

abstract：:Hydroxyurea (HU), an inhibitor of DNA synthesis, can also induce haemoglobinization in certain erythroid cell lines. In this study, we report that intracellular peroxides levels were increased in HU-treated murine erythroleukaemia (MEL) cells and that l-acetyl-N-cysteine (LNAC), a potent reducing reagent, had a signif...

journal_title：British journal of haematology

authors： Nagai T,Tarumoto T,Miyoshi T,Ohmine K,Muroi K,Komatsu N,Sassa S,Ozawa K

更新日期：2003-05-01 00:00:00

abstract：:The regulatory human immunodeficiency virus-1 (HIV-1) Tat protein shows pleiotropic effects on the survival and growth of both HIV-1-infected and uninfected CD4+ T lymphocytes. In this study, we have demonstrated that low concentrations (10 ng/ml) of extracellular Tat protein induce the expression of both c-fos mRNA a...

journal_title：British journal of haematology

authors： Gibellini D,Re MC,Ponti C,Celeghini C,Melloni E,La Placa M,Zauli G

更新日期：2001-03-01 00:00:00

abstract：:Refractory anaemia with ring sideroblasts (RARS) is distinguished by hyperplastic inefficient erythropoiesis, aberrant mitochondrial ferritin accumulation and anaemia. Heterozygous mutations in the spliceosome gene SF3B1 are found in a majority of RARS cases. To explore the link between SF3B1 mutations and anaemia, we...

journal_title：British journal of haematology

authors： Conte S,Katayama S,Vesterlund L,Karimi M,Dimitriou M,Jansson M,Mortera-Blanco T,Unneberg P,Papaemmanuil E,Sander B,Skoog T,Campbell P,Walfridsson J,Kere J,Hellström-Lindberg E

更新日期：2015-11-01 00:00:00

abstract：:Two children affected by severe aplastic anaemia (SAA) underwent allogeneic bone marrow transplantation (BMT) using partially matched family donors. In both cases there was a successful engraftment of donor haemopoietic stem cells. However, after an initial erythropoietic recovery, 5 months following BMT both children...

journal_title：British journal of haematology

authors： Locatelli F,Pedrazzoli P,Barosi G,Zecca M,Porta F,Liberato L,Gambarana D,Nespoli L,Cazzola M

更新日期：1992-04-01 00:00:00

abstract：:We present an immunocompetent patient with transfusion-associated graft-versus-host disease (GVHD), in which chimaerism of peripheral blood lymphocytes was demonstrated by analysis of a highly polymorphic genome. The patient was treated successfully with anti-CD3 monoclonal antibody, OKT3 and cyclosporin A. Although i...

journal_title：British journal of haematology

authors： Yasukawa M,Shinozaki F,Hato T,Takada K,Ishii Y,Tamai T,Takamatsu H,Shiobara S,Yoshizaki K,Fujita S

更新日期：1994-04-01 00:00:00

abstract：:New therapeutic approaches are needed to improve the cure rates in acute myeloid leukaemia (AML). The present study was designed to investigate whether: (1) cytotoxic lymphocytes could be expanded from AML patients in complete remission; (2) their signal transduction machinery was preserved; (3) these cells were capab...

journal_title：British journal of haematology

authors： Torelli GF,Guarini A,Palmieri G,Breccia M,Vitale A,Santoni A,Foa R

更新日期：2002-02-01 00:00:00

abstract：:The molecular basis of the three major alleles (Fy(a)/Fy(b)/Fy) of the Duffy (FY) blood group system has recently been established but the Fy(x) phenotype associated with weak expression of the Fy(b) and other FY antigens is poorly understood. In the Fy(x) genes of five unrelated British and Swedish donors with the Fy...

journal_title：British journal of haematology

authors： Olsson ML,Smythe JS,Hansson C,Poole J,Mallinson G,Jones J,Avent ND,Daniels G

更新日期：1998-12-01 00:00:00

abstract：:Erythroid colonies derived from the circulating early erythroid precursor (BFU-E) of a patient with congenital dyserythropoietic anaemia type I (CDA I) have been grown in plasma clot and studied by electron microscopy. The number of circulating BFU-E was in the normal range with a roughly normal appearance at the ligh...

journal_title：British journal of haematology

authors： Vainchenker W,Guichard J,Bouguet J,Breton-Gorius J

更新日期：1980-09-01 00:00:00

abstract：:The inducibility of glycosyl-phosphatidylinositol (GPI)-anchored proteins on affected paroxysmal nocturnal haemoglobinuria (PNH) neutrophils (PMN) after both in vitro and in vivo stimulation was investigated. Fc gamma R-III (CD16), decay-accelerating factor (DAF/CD55) and 20 kD homologous restriction factor (HRF20/CD5...

journal_title：British journal of haematology

authors： Ninomiya H,Muraki Y,Shibuya K,Nagasawa T,Abe T

更新日期：1993-07-01 00:00:00

abstract：:Among 80 hereditary spherocytosis (HS) kindreds studied using denaturing electrophoretic separation of solubilized eythrocyte membrane proteins, we recognized three prominent subsets: HS with isolated spectrin deficiency, HS with combined spectrin and ankyrin deficiency, and HS with band 3 deficiency These three subse...

journal_title：British journal of haematology

authors： Dhermy D,Galand C,Bournier O,Boulanger L,Cynober T,Schismanoff PO,Bursaux E,Tchernia G,Boivin P,Garbarz M

更新日期：1997-07-01 00:00:00

abstract：:Heterozygosity for a C8524T transition in the protein C gene converting Ser270(TCG) to Leu(TTG) in the protease domain was identified in a family with venous thrombosis. The mutation was associated with parallel reduction in plasma levels of protein C anticoagulant activity and protein C antigen, which is consistent w...

journal_title：British journal of haematology

authors： Lind B,Koefoed P,Thorsen S

更新日期：2001-06-01 00:00:00

abstract：:A case of acute myeloid leukaemia (AML) occurring in a patient with untreated chronic lymphatic leukaemia (CLL) is presented. The diagnosis of two simultaneous leukaemic processes is based on morphological, cytochemical and immunological findings. The significance of the development of AML in patients with CLL is disc...

journal_title：British journal of haematology

authors： Lawlor E,McCann SR,Whelan A,Greally J,Temperley IJ

更新日期：1979-11-01 00:00:00

abstract：:The efficiency of stored platelet transfusion was evaluated in terms of clinical status in 141 thrombocytopenic patients. In a paired prospective study in which fresh platelets were used as controls, clinical efficiency was assessed on the basis of the ability to increase platelet count (recovery) and the time to the ...

journal_title：British journal of haematology

authors： Norol F,Kuentz M,Cordonnier C,Beaujean F,Haioun C,Vernant JP,Duédari N

更新日期：1994-01-01 00:00:00

abstract：:Cytogenetic deletions of the short arm of chromosome 12 are common recurring alterations found in a wide range of haematological neoplasias, including childhood acute lymphoblastic leukaemia (ALL), the most frequent paediatric malignancy. Such a loss of genetic material suggests the presence of a tumour suppressor gen...

journal_title：British journal of haematology

authors： Baccichet A,Sinnett D

更新日期：1997-10-01 00:00:00

abstract：:This study systematically reviewed and meta-analysed the prognostic value of complete remission status at end-of-treatment (18) F-fluoro-2-deoxy-d-glucose positron emission tomography (FDG-PET) in diffuse large B-cell lymphoma (DLBCL) patients treated with rituximab, cyclophosphamide, doxorubicin, vincristine and pred...

journal_title：British journal of haematology

authors： Adams HJ,Nievelstein RA,Kwee TC

更新日期：2015-07-01 00:00:00

abstract：:To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar...

journal_title：British journal of haematology

authors： Mihara K,Imai C,Coustan-Smith E,Dome JS,Dominici M,Vanin E,Campana D

更新日期：2003-03-01 00:00:00

abstract：:Platelets in an infant with Chediak-Higashi (C-H) syndrome without bleeding manifestations and not in the accelerated phase showed abnormal function consistent with storage pool disorder as shown by abnormal aggregation, decreased storage capacity and release of [14C]5-HT, low endogenous 5-HT, reduced ATP and ADP with...

journal_title：British journal of haematology

authors： Boxer GJ,Holmsen H,Robkin L,Bang NU,Boxer LA,Baehner RL

更新日期：1977-04-01 00:00:00

abstract：:After T-cell depleted marrow transplantation, there is a rapid recovery of cytotoxic effector cells, with activity against targets not susceptible to killing by 'resting' natural killer cells. These targets include Epstein-Barr virus transformed B cells and leukaemic cell lines. Activated killer cell function declines...

journal_title：British journal of haematology

authors： Leger O,Drexler HG,Reittie JE,Secker-Walker L,Prentice HG,Brenner MK

更新日期：1987-11-01 00:00:00

abstract：:The primary immune response to a viral antigen (tick-borne encephalitis, TBE) has been determined in haemophiliacs. Twelve HIV-negative and four clinically asymptomatic, HIV-positive haemophiliacs as well as 16 age-matched healthy controls were included in the study. Antibody responses after TBE vaccination were compa...

journal_title：British journal of haematology

authors： Wolf HM,Pum M,Jáger R,István L,Mannhalter JW,Eibl MM

更新日期：1992-10-01 00:00:00

abstract：:Microcirculatory disorders are a common finding in sepsis. We have analysed the influence of two factors released in sepsis, endotoxin and tumour necrosis factor (TNF), on rheological properties of blood cells. The deformability of mixed cell suspensions, isolated erythrocytes, mononuclear cells, or polymorphonuclear ...

journal_title：British journal of haematology

authors： Betticher DC,Keller H,Maly FE,Reinhart WH

更新日期：1993-01-01 00:00:00

abstract：:Patients with polycythaemia vera and secondary polycythaemia studied with 125I-labelled fibrinogen, had a significantly increased plasma fibrinogen turnover. The abnormalities were greater in polycythaemia vera and correlated with platelet count but not with packed cell volume. In six patients with polycythaemia vera,...

journal_title：British journal of haematology

authors： Boughton BJ,Dallinger KJ

更新日期：1983-01-01 00:00:00

abstract：:Imatinib mesylate (IM, STI 571, Glivec) can induce a high rate of complete cytogenetic response (CCR) in chronic myeloid leukaemia (CML) patients, although to date the majority of patients continue to have detectable disease by sensitive reverse transcription polymerase chain reaction (RT-PCR). It is therefore possibl...

journal_title：British journal of haematology

authors： Drummond MW,Marin D,Clark RE,Byrne JL,Holyoake TL,Lennard A,United Kingdom Chronic Myeloid Leukaemia (UK CML) Working Party.

更新日期：2003-11-01 00:00:00

abstract：:Relapse of childhood acute lymphoblastic leukaemia (ALL) involving the eye is a rare but challenging problem. Twenty cases occurred in patients treated on the Medical Research Council United Kingdom Acute Lymphoblastic Leukaemia XI and ALL97 trials between 1991 and 2001, representing 2.2% of ALL relapses. Seventeen oc...

journal_title：British journal of haematology

authors： Somervaille TC,Hann IM,Harrison G,Eden TO,Gibson BE,Hill FG,Mitchell C,Kinsey SE,Vora AJ,Lilleyman JS,MRC Childhood Leukaemia Working Party.

更新日期：2003-04-01 00:00:00

abstract：:To investigate the relationship between normal B-cells, B-cell chronic lymphocytic leukaemia (B-CLL) cells and hairy cell leukaemia (HCL) cells the three cell types were incubated with phorbol myristic acetate (PMA). The parameters studied were morphology, immunophenotype and tartrate resistant acid phosphatase (TRAP)...

journal_title：British journal of haematology

authors： Visser L,Poppema S

更新日期：1990-07-01 00:00:00

abstract：:We have previously shown that polymorphonuclear leucocytes (PMN) harvested from children with cancer and exposed to chemotherapy exhibit defective bactericidal activities against both Gram-positive and Gram-negative microorganisms as well as accelerated apoptosis. In this study, PMN from children with cancer were eval...

journal_title：British journal of haematology

authors： Lejeune M,Cantinieaux B,Harag S,Ferster A,Devalck C,Sariban E

更新日期：1999-09-01 00:00:00

abstract：:The 3q21q26 syndrome leukaemias are characterised by dystrophic megakaryocytes, elevated platelet counts, ectopic EVI1 protein production and poor prognosis. To investigate the molecular basis of this disease, we developed a model system to examine the biological activity of EVI1 in a megakaryocyte progenitor cell lin...

journal_title：British journal of haematology

authors： Kilbey A,Alzuherri H,McColl J,Calés C,Frampton J,Bartholomew C

更新日期：2005-09-01 00:00:00

abstract：:In an attempt to develop a model system for analysing iron metabolism in a relatively homogeneous population of early red cell precursors, the intracellular distribution of 59Fe was examined in Friend murine erythroleukaemic cells after induction of haemoglobin synthesis with dimethylsulphoxide. After incubation of th...

journal_title：British journal of haematology

authors： Peto TE,Rutherford TR,Thompson JL,Weatherall DJ

更新日期：1983-08-01 00:00:00

abstract：:The iron chelator deferasirox (DFX) prevents complications related to transfusional iron overload in several haematological disorders characterized by marrow failure. It is also able to induce haematological responses in a percentage of treated patients, particularly in those affected by myelodysplastic syndromes. The...

journal_title：British journal of haematology

authors： Tataranni T,Agriesti F,Mazzoccoli C,Ruggieri V,Scrima R,Laurenzana I,D'Auria F,Falzetti F,Di Ianni M,Musto P,Capitanio N,Piccoli C

更新日期：2015-07-01 00:00:00

abstract：:The changes in bone marrow (BM) stem cell reserve and function and stromal cell function in patients with active systemic lupus erythematosus (SLE) were investigated. The study was carried out on seven SLE patients and 28 healthy controls using flow cytometry and in vitro cell culture assays. We found that patients ha...

journal_title：British journal of haematology

authors： Papadaki HA,Boumpas DT,Gibson FM,Jayne DR,Axford JS,Gordon-Smith EC,Marsh JC,Eliopoulos GD

更新日期：2001-10-01 00:00:00