Abstract:
:To investigate the relationship between normal B-cells, B-cell chronic lymphocytic leukaemia (B-CLL) cells and hairy cell leukaemia (HCL) cells the three cell types were incubated with phorbol myristic acetate (PMA). The parameters studied were morphology, immunophenotype and tartrate resistant acid phosphatase (TRAP). PMA stimulation of B-cells induced morphological changes as well as CD5, CD11c, B-ly 7 and TRAP positivity. The cells formed small aggregates, the cell membranes were ruffled and frequently hairy and a small number of cells became plastic adherent and developed dendritic structure. CD5 and CD11c appeared on day 2, imitating a B-CLL phenotype. On day 3 and 4 a decrease of CD5, an increase of CD11c and the appearance of B-ly 7 could be seen mimicking an HCL phenotype. The changes in B-CLL and HCL upon PMA stimulation were mainly morphological: the B-CLL cells became ruffled and aggregated strongly, while the HCL cells developed dendritic features and became adherent. The immunophenotype of the PMA stimulated HCL cells did not change. The B-CLL cells remained CD5 positive and did not become B-ly 7 positive. The findings indicate that although PMA stimulation of normal peripheral blood cells results in CLL- as well as HCL-like phenotypes, similar stimulation of B-CLL cells does not result in an HCL-like phenotype.
journal_name
Br J Haematoljournal_title
British journal of haematologyauthors
Visser L,Poppema Sdoi
10.1111/j.1365-2141.1990.tb04349.xsubject
Has Abstractpub_date
1990-07-01 00:00:00pages
359-65issue
3eissn
0007-1048issn
1365-2141journal_volume
75pub_type
杂志文章abstract::Neutralizing anti-IFN alpha antibodies (nIFN alpha Abs) occur in a significant proportion of patients with hairy cell leukaemia, hepatitis or solid tumours treated with recombinant IFN alpha (IFN alpha 2a or IFN alpha 2b), but information on their incidence in chronic myeloid leukaemia (CML) is scanty and their clinic...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.1996.d01-1790.x
更新日期:1996-08-01 00:00:00
abstract::Mantle cell lymphoma (MCL) is a heterogenic non-Hodgkin lymphoma entity, with a median survival of about 5 years. In 2008 we reported the early - based on the median observation time of 4 years - results of the Nordic Lymphoma Group MCL2 study of frontline intensive induction immunochemotherapy and autologous stem cel...
journal_title:British journal of haematology
pub_type: 临床试验,杂志文章
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更新日期:2012-08-01 00:00:00
abstract::Massive genomic analyses have underscored the diversity of chronic lymphocytic leukaemia (CLL) between patients. Genetic heterogeneity of tumour clones within a patient may fuel tumour evolution. Several recurrently deregulated intra-cellular pathways are candidates for targeted therapies that are very promising and a...
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pub_type: 杂志文章,评审
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.2001.02508.x
更新日期:2001-01-01 00:00:00
abstract::To determine the long-term efficacy of FLT3 inhibitors (FLT3i) in the salvage setting for relapsed and refractory (rel/ref) acute myeloid leukemia (AML) with FLT3 internal tandem duplication (AML FLT3-ITD), we conducted a retrospective study of 120 patients with rel/ref AML FLT3-ITD who received salvage therapy with e...
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journal_title:British journal of haematology
pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.1996.d01-1961.x
更新日期:1996-12-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1986-07-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:British journal of haematology
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1990.tb02627.x
更新日期:1990-05-01 00:00:00
abstract::The response to 0.8 ml of D-positive (ccDEE) red cells injected i.v., together with 1 microgram anti-D i.m., was studied in 13 D-negative male volunteers (test subjects); the red cells were labelled with 51Cr and were found to have a mean survival time of 24 d, after correction for Cr elution. Within the following 5 m...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1983.tb01997.x
更新日期:1983-01-01 00:00:00
abstract::Erythroid regeneration is an important and separate element in the engraftment process in allogeneic and autologous bone marrow transplantation (alloBMT, autoBMT). Qualitative visual reticulocyte counting has proved inadequate in the evaluation of erythropoiesis after BMT but automated flow cytometry now allows the re...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1992.tb08163.x
更新日期:1992-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2141.1982.tb03913.x
更新日期:1982-11-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:British journal of haematology
pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.1998.00867.x
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abstract::A rapid, non-radioactive, PCR-based method to genotype the XbaI restriction fragment length polymorphism of the human factor VIII gene is described. The method uses long-distance PCR followed by XbaI restriction digestion and agarose gel electrophoresis. The 6.6 kb amplification product includes a constant XbaI site, ...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.1999.01731.x
更新日期:1999-12-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/bjh.15195
更新日期:2018-05-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.2008.07236.x
更新日期:2008-08-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1989-10-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章,多中心研究
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更新日期:2000-03-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.2001.02946.x
更新日期:2001-08-01 00:00:00
abstract::In acute promyelocytic leukaemia (APL), differentiation therapy can be complicated by the development of a differentiation syndrome (DS). Pulmonary infiltration of differentiating leukaemic cells is a key event in the development of DS. Several mediators have been identified that may promote migration and extravasatio...
journal_title:British journal of haematology
pub_type: 杂志文章,评审
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更新日期:2010-11-01 00:00:00
abstract::The organelle pathology of neutrophils in chronic granulocytic leukaemia (CGL) was investigated by analytical subcellular fractionation. There were minor reductions in activity of some granule enzymes with an abnormal distribution in sucrose density gradients of the specific granules. There was a marked reduction of 5...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1979.tb05891.x
更新日期:1979-04-01 00:00:00
abstract::Umbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34+ cells derived from human umbilical cord blood. During CD34+ cell enrichment, includi...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.2007.06606.x
更新日期:2007-06-01 00:00:00