当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Tn5/IS50 target recognition.

Tn5/IS50 target recognition.

Abstract：

:This communication reports an analysis of Tn5/IS50 target site selection by using an extensive collection of Tn5 and IS50 insertions in two relatively small regions of DNA (less than 1 kb each). For both regions data were collected resulting from in vitro and in vivo transposition events. Since the data sets are consistent and transposase was the only protein present in vitro, this demonstrates that target selection is a property of only transposase. There appear to be two factors governing target selection. A target consensus sequence, which presumably reflects the target selection of individual pairs of Tn5/IS50 bound transposase protomers, was deduced by analyzing all insertion sites. The consensus Tn5/IS50 target site is A-GNTYWRANC-T. However, we observed that independent insertion sites tend to form groups of closely located insertions (clusters), and insertions very often were spaced in a 5-bp periodic fashion. This suggests that Tn5/IS50 target selection is facilitated by more than two transposase protomers binding to the DNA, and, thus, for a site to be a good target, the overlapping neighboring DNA should be a good target, too. Synthetic target sequences were designed and used to test and confirm this model.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Goryshin IY,Miller JA,Kil YV,Lanzov VA,Reznikoff WS

doi

10.1073/pnas.95.18.10716

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

10716-21

issue

18

eissn

0027-8424

issn

1091-6490

journal_volume

95

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Genetic and pharmacological disruption of neurokinin 1 receptor function decreases anxiety-related behaviors and increases serotonergic function.

    abstract：:Alterations in serotonin (5-hydroxytriptamine, 5-HT), norepinephrine, and gamma-aminobutyric acid have been linked to the pathophysiology of anxiety and depression, and medications that modulate these neurotransmitters are widely used to treat mood disorders. Recently, the neuropeptide substance P (SP) and its recepto...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.041596398

    authors： Santarelli L,Gobbi G,Debs PC,Sibille ET,Blier P,Hen R,Heath MJ

    更新日期：2001-02-13 00:00:00

  • Rhesus monkeys know when they remember.

    abstract：:Humans are consciously aware of some memories and can make verbal reports about these memories. Other memories cannot be brought to consciousness, even though they influence behavior. This conspicuous difference in access to memories is central in taxonomies of human memory systems but has been difficult to document i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.071600998

    authors： Hampton RR

    更新日期：2001-04-24 00:00:00

  • Random circular permutation of genes and expressed polypeptide chains: application of the method to the catalytic chains of aspartate transcarbamoylase.

    abstract：:Recent studies on proteins whose N and C termini are in close proximity have demonstrated that folding of polypeptide chains and assembly of oligomers can be accomplished with circularly permuted chains. As yet no methodical study has been conducted to determine how extensively new termini can be introduced and where ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.21.11591

    authors： Graf R,Schachman HK

    更新日期：1996-10-15 00:00:00

  • Field experiments of success-breeds-success dynamics.

    abstract：:Seemingly similar individuals often experience drastically different success trajectories, with some repeatedly failing and others consistently succeeding. One explanation is preexisting variability along unobserved fitness dimensions that is revealed gradually through differential achievement. Alternatively, positive...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,随机对照试验

    doi：10.1073/pnas.1316836111

    authors： van de Rijt A,Kang SM,Restivo M,Patil A

    更新日期：2014-05-13 00:00:00

  • IL-33 exacerbates antigen-induced arthritis by activating mast cells.

    abstract：:IL-33, a cytokine of the IL-1 family, is closely associated with type II T cell responses. Here, we report an unexpected proinflammatory role of IL-33 in inflammatory arthritis. IL-33 was expressed in synovial fibroblasts from patients with rheumatoid arthritis (RA). Expression was markedly elevated in vitro by inflam...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0801898105

    authors： Xu D,Jiang HR,Kewin P,Li Y,Mu R,Fraser AR,Pitman N,Kurowska-Stolarska M,McKenzie AN,McInnes IB,Liew FY

    更新日期：2008-08-05 00:00:00

  • A chimeric adenovirus vector encoding reovirus attachment protein sigma1 targets cells expressing junctional adhesion molecule 1.

    abstract：:The utility of adenovirus (Ad) vectors for gene transduction can be limited by receptor specificity. We developed a gene-delivery vehicle in which the potent Ad5 vector was genetically reengineered to display the mucosal-targeting sigma1 protein of reovirus type 3 Dearing (T3D). A sigma1 construct containing all but a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400542101

    authors： Mercier GT,Campbell JA,Chappell JD,Stehle T,Dermody TS,Barry MA

    更新日期：2004-04-20 00:00:00

  • Model for the structure of the lipid bilayer.

    abstract：:A detailed model for the structure and dynamics of the interior of the lipid bilayer in the liquid crystal phase is presented. The model includes two classes of motion: (i) the internal dynamics of the chains, determined from Brownian dynamics simulations with a continuous version of the Marcelja mean-field potential,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.3.892

    authors： Pastor RW,Venable RM,Karplus M

    更新日期：1991-02-01 00:00:00

  • Reaction of cyanide with cytochrome ba3 from Thermus thermophilus: spectroscopic characterization of the Fe(II)a3-CN.Cu(II)B-CN complex suggests four 14N atoms are coordinated to CuB.

    abstract：:Cytochrome ba3 from Thermus thermophilus reacts slowly with excess HCN at pH 7.4 to create a form of the enzyme in which CuA, cytochrome b, and CuB remain oxidized, while cytochrome a3 is reduced by one electron, presumably with the formation of cyanogen. We have examined this form of the enzyme by UV-visible, resonan...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.8.3195

    authors： Surerus KK,Oertling WA,Fan C,Gurbiel RJ,Einarsdóttir O,Antholine WE,Dyer RB,Hoffman BM,Woodruff WH,Fee JA

    更新日期：1992-04-15 00:00:00

  • Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.

    abstract：:Most proteins are in equilibrium with partially and globally unfolded conformations. In contrast, kinetically stable proteins (KSPs) are trapped by an energy barrier in a specific state, unable to transiently sample other conformations. Among many potential roles, it appears that kinetic stability (KS) is a feature us...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0705417104

    authors： Xia K,Manning M,Hesham H,Lin Q,Bystroff C,Colón W

    更新日期：2007-10-30 00:00:00

  • Functional identification of a neurocircuit regulating blood glucose.

    abstract：:Previous studies implicate the hypothalamic ventromedial nucleus (VMN) in glycemic control. Here, we report that selective inhibition of the subset of VMN neurons that express the transcription factor steroidogenic-factor 1 (VMN(SF1) neurons) blocks recovery from insulin-induced hypoglycemia whereas, conversely, activ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1521160113

    authors： Meek TH,Nelson JT,Matsen ME,Dorfman MD,Guyenet SJ,Damian V,Allison MB,Scarlett JM,Nguyen HT,Thaler JP,Olson DP,Myers MG Jr,Schwartz MW,Morton GJ

    更新日期：2016-04-05 00:00:00

  • Acrosin is essential for sperm penetration through the zona pellucida in hamsters.

    abstract：:During natural fertilization, mammalian spermatozoa must pass through the zona pellucida before reaching the plasma membrane of the oocyte. It is assumed that this step involves partial lysis of the zona by sperm acrosomal enzymes, but there has been no unequivocal evidence to support this view. Here we present eviden...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1917595117

    authors： Hirose M,Honda A,Fulka H,Tamura-Nakano M,Matoba S,Tomishima T,Mochida K,Hasegawa A,Nagashima K,Inoue K,Ohtsuka M,Baba T,Yanagimachi R,Ogura A

    更新日期：2020-02-04 00:00:00

  • Long-range compaction and flexibility of interphase chromatin in budding yeast analyzed by high-resolution imaging techniques.

    abstract：:Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase chromatin in budding yeast. Unlike previous studies, ours examines nonrepetitive chromatin at intervals short enough to...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0402766101

    authors： Bystricky K,Heun P,Gehlen L,Langowski J,Gasser SM

    更新日期：2004-11-23 00:00:00

  • Optimal stomatal behavior with competition for water and risk of hydraulic impairment.

    abstract：:For over 40 y the dominant theory of stomatal behavior has been that plants should open stomates until the carbon gained by an infinitesimal additional opening balances the additional water lost times a water price that is constant at least over short periods. This theory has persisted because of its remarkable succes...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1615144113

    authors： Wolf A,Anderegg WR,Pacala SW

    更新日期：2016-11-15 00:00:00

  • Protein kinases C-beta and C-epsilon link the mast cell high-affinity receptor for IgE to the expression of c-fos and c-jun.

    abstract：:In this report we identify the specific isozymes of protein kinase C (PKC) that are involved in c-fos and c-jun mRNA accumulation in the rat basophilic leukemia cell line RBL-2H3. These cells could be largely depleted of the endogenous PKC isozymes by chronic treatment with phorbol 12-myristate 13-acetate followed by ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.16.7722

    authors： Razin E,Szallasi Z,Kazanietz MG,Blumberg PM,Rivera J

    更新日期：1994-08-02 00:00:00

  • Conserved residues in the Plasmodium vivax Duffy-binding protein ligand domain are critical for erythrocyte receptor recognition.

    abstract：:Malaria merozoite invasion of human erythrocytes depends on recognition of specific erythrocyte surface receptors by parasite ligands. Plasmodium vivax merozoite invasion is totally dependent on the recognition of the Duffy blood group antigen by the parasite ligand Duffy-binding protein (DBP). Receptor recognition by...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0405421101

    authors： VanBuskirk KM,Sevova E,Adams JH

    更新日期：2004-11-02 00:00:00

  • Interaction of purified type IIB von Willebrand factor with the platelet membrane glycoprotein Ib induces fibrinogen binding to the glycoprotein IIb/IIIa complex and initiates aggregation.

    abstract：:Von Willebrand factor (vWF) was purified from the plasma of a patient with type IIB von Willebrand disease (vWF from such a patient, IIB vWF) who had a normal platelet count and showed no evidence of spontaneous platelet aggregation. Large multimers of IIB vWF were absent from purified preparations and from plasma. Ri...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.21.7424

    authors： De Marco L,Girolami A,Zimmerman TS,Ruggeri ZM

    更新日期：1985-11-01 00:00:00

  • Insulin binding and insulin action in cultured fibroblasts: significant differences between a phosphoglucose isomerase-deficient mutant and the parental strain.

    abstract：:The interrelationships of hexose feeding and insulin action were studied in the Chinese hamster fibroblast cell lines 023 and DS-7. The latter, derived from 023 and deficient in phosphoglucose isomerase, has been used to map the metabolic requirements for aldohexose-mediated down-regulation or "curbing" of hexose tran...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.11.3684

    authors： Wilson C,Peterson SW,Ullrey DB,Kalckar HM

    更新日期：1986-06-01 00:00:00

  • Epitopes of proteoglycans eliciting an anti-proteoglycan response in chronic immune synovitis.

    abstract：:This study details the immune response to cartilage proteoglycan in experimental chronic IgG-induced immune synovitis. Antibodies reactive with purified rabbit proteoglycan monomer were observed in nine of nine rabbits with immune synovitis. IgG-immunized but nonsynovitic control animals with no pathology showed no an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.3.832

    authors： Yoo JU,Kresina TF,Malemud CJ,Goldberg VM

    更新日期：1987-02-01 00:00:00

  • Phospholipase DZ2 plays an important role in extraplastidic galactolipid biosynthesis and phosphate recycling in Arabidopsis roots.

    abstract：:Low phosphate (Pi) availability is one of the major constraints for plant productivity in natural and agricultural ecosystems. Plants have evolved a myriad of developmental and biochemical mechanisms to increase internal Pi uptake and utilization efficiency. One important biochemical pathway leading to an increase in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0600863103

    authors： Cruz-Ramírez A,Oropeza-Aburto A,Razo-Hernández F,Ramírez-Chávez E,Herrera-Estrella L

    更新日期：2006-04-25 00:00:00

  • Reciprocity or near-reciprocity of highly coupled enzymatic processes at the multidimensional inflection point.

    abstract：:It has recently been demonstrated that coupled enzymatic processes may possess, for a particular choice of the state variables, multidimensional inflection points in thermodynamic force-flow space. The conditions for reciprocity in the linear region near such a reference state, which may be far from equilibrium, are o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.7.4314

    authors： Caplan SR

    更新日期：1981-07-01 00:00:00

  • Formation of a translational inhibitor by interaction of phospholipid with the eukaryotic initiation factor 2.

    abstract：:The polypeptide chain initiation factor 2 (eIF-2) binds phospholipid (PL) and becomes a potent inhibitor of translation in hemin-supplemented reticulocyte lysates. This binding is markedly reduced by prior treatment of eIF-2 with N-ethylmaleimide. Although PL is probably bound by all three eIF-2 subunits, our results ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.18.6711

    authors： de Haro C,de Herreros AG,Ochoa S

    更新日期：1986-09-01 00:00:00

  • The molecular basis for the high photosensitivity of rhodopsin.

    abstract：:Based on structural information derived from the F NMR data of labeled rhodopsins, rhodopsin crystal structure, and excited-state properties of model polyenes, we propose a molecular mechanism that accounts specifically for the causes of the well-known enhanced photoreactivity of rhodopsin (increased rates and quantum...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2536769100

    authors： Liu RS,Colmenares LU

    更新日期：2003-12-09 00:00:00

  • Vitamin E reduces chromosomal damage and inhibits hepatic tumor formation in a transgenic mouse model.

    abstract：:We have previously shown that chronic activation of mitogenic signaling induced by over-expression of c-myc and transforming growth factor-alpha (TGFalpha) transgenes in mouse liver induces a state of oxidative stress. We therefore proposed that increased reactive oxygen species (ROS) generation might be responsible f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.040428797

    authors： Factor VM,Laskowska D,Jensen MR,Woitach JT,Popescu NC,Thorgeirsson SS

    更新日期：2000-02-29 00:00:00

  • Erythroid cell differentiation: murine erythroleukemia cell variant with unique pattern of induction by polar compounds.

    abstract：:The murine-virus-infected erythroleukemia cell system provides an opportunity to examine regulatory mechanisms controlling cytodifferentiation. A cloned cell line (DR10c3) resistant to the erythropoiesis-inducing effect of dimethylsulfoxide (Me2SO) was isolated from the Me2SO-sensitive line DS19. DR10c3 is characteriz...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.4.1232

    authors： Ohta Y,Tanaka M,Terada M,Miller OJ,Bank A,Marks P,Rifkind RA

    更新日期：1976-04-01 00:00:00

  • Synthesis of catalase in two cell-free protein-synthesizing systems and in rat liver.

    abstract：:Rat liver polysomal RNA was translated in the rabbit reticulocyte lysate and in the wheat germ cell-free protein-synthesizing systems, using [(35)S]methionine as label. The catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase, EC 1.11.1.6) that was synthesized was isolated by immunoprecipitation and characteri...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.9.4344

    authors： Robbi M,Lazarow PB

    更新日期：1978-09-01 00:00:00

  • The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis.

    abstract：:GPIbalpha binding to von Willebrand factor (VWF) exposed at a site of vascular injury is thought to be the first step in the formation of a hemostatic plug. However, our previous studies in VWF-deficient mice demonstrated delayed but not absent arterial thrombus formation, suggesting that, under these conditions, GPIb...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0608207103

    authors： Bergmeier W,Piffath CL,Goerge T,Cifuni SM,Ruggeri ZM,Ware J,Wagner DD

    更新日期：2006-11-07 00:00:00

  • p70S6 kinase signals cell survival as well as growth, inactivating the pro-apoptotic molecule BAD.

    abstract：:Cytokines often deliver simultaneous, yet distinct, cell growth and cell survival signals. The 70-kDa ribosomal protein S6 kinase (p70S6K) is known to regulate cell growth by inducing protein synthesis components. We purified membrane-based p70S6K as a kinase responsible for site-specific phosphorylation of BAD, which...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.171301998

    authors： Harada H,Andersen JS,Mann M,Terada N,Korsmeyer SJ

    更新日期：2001-08-14 00:00:00

  • Self-removal of condensed water on the legs of water striders.

    abstract：:The ability to control drops and their movements on phobic surfaces is important in printing or patterning, microfluidic devices, and water-repellent materials. These materials are always micro-/nanotextured, and a natural limitation of repellency occurs when drops are small enough (as in a dew) to get trapped in the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1506874112

    authors： Wang Q,Yao X,Liu H,Quéré D,Jiang L

    更新日期：2015-07-28 00:00:00

  • Expression profiling of pancreatic beta cells: glucose regulation of secretory and metabolic pathway genes.

    abstract：:Pancreatic beta cells respond to changes in blood glucose by secreting insulin and increasing insulin synthesis. To identify genes used in these responses, we have carried out expression profiling of beta cells exposed to high (25 mM) or low (5.5 mM) glucose by using oligonucleotide microarrays. Functional clustering ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.100126597

    authors： Webb GC,Akbar MS,Zhao C,Steiner DF

    更新日期：2000-05-23 00:00:00

  • Long noncoding RNA NEAT1 (nuclear paraspeckle assembly transcript 1) is critical for phenotypic switching of vascular smooth muscle cells.

    abstract：:In response to vascular injury, vascular smooth muscle cells (VSMCs) may switch from a contractile to a proliferative phenotype thereby contributing to neointima formation. Previous studies showed that the long noncoding RNA (lncRNA) NEAT1 is critical for paraspeckle formation and tumorigenesis by promoting cell proli...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1803725115

    authors： Ahmed ASI,Dong K,Liu J,Wen T,Yu L,Xu F,Kang X,Osman I,Hu G,Bunting KM,Crethers D,Gao H,Zhang W,Liu Y,Wen K,Agarwal G,Hirose T,Nakagawa S,Vazdarjanova A,Zhou J

    更新日期：2018-09-11 00:00:00