Abstract:
:The aims of the present study were to establish whether vasoactive intestinal polypeptide (VIP) could mobilize internally-stored Ca2+ and whether Ca2+ release could trigger Ca2+ influx from the extracellular space. Bovine pulmonary artery endothelial cells from an established cell line were loaded with fura-2/AM and cells were studied in suspension or were imaged in monolayers at 40-80% confluency. In Ca2+ imaging studies, VIP evoked Ca2+ transients in Ca2+-free medium containing 50 microM EGTA. This was observed in 33 out of 122 cells examined on 29 separate trials. With each cell, the spread of Ca2+ appeared to occur from the periphery of the cell to the central core. Cells which did not respond to VIP responded to other stimulants such as bradykinin, endoplasmic reticulum Ca2+ pump inhibitors, (cyclopiazonic acid and thapsigargin), and endoplasmic reticulum Ca2+ release channel opener, ryanodine. The reintroduction of Ca2+ following VIP-induced Ca2+ release did not evoke a Ca2+ response in 5 cells imaged. Cells in suspension showed typical biphasic responses to bradykinin, thapsigargin or cyclopiazonic acid in the presence of external Ca2+. Stimulation with VIP caused transient Ca2+ responses in Ca2+-free physiological saline containing 50 microM EGTA. However, only 1 out of 4 cells tested showed a response to Ca2+ when it was reintroduced to the bathing medium. This study provided direct evidence for the first time in these bovine endothelial cells for VIP-mediated elevation of cytosolic concentration of Ca2+. The results also suggested that other mechanisms might prevail preventing capacitative Ca2+ entry following the release of internally-stored Ca2+.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Low AM,Sormaz L,Kwan CY,Daniel EEdoi
10.1016/s0014-2999(97)01315-0subject
Has Abstractpub_date
1997-11-27 00:00:00pages
227-35issue
2-3eissn
0014-2999issn
1879-0712pii
S0014-2999(97)01315-0journal_volume
339pub_type
杂志文章abstract::RES-701-1 (cyclic (Gly1-Asp9)(Gly-Asn-Trp-His-Gly-Thr-Ala-Pro-Asp-Trp-Phe- Phe-Asn-Tyr-Tyr-Trp)), a peptide isolated from Streptomyces sp., has been reported to inhibit the endothelin ETB receptor. We examined the effects of this peptide on the blood vessels and the small intestine. In isolated rat aorta without endot...
journal_title:European journal of pharmacology
pub_type: 杂志文章
doi:10.1016/0014-2999(94)90739-0
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abstract::The effects of adenyl compounds and some of their analogues were examined on atrial and ventricular muscle of Xenopus laevis. In contrast to all heart muscle preparations studied previously, adenosine produced excitation of the ventricle of Xenopus. 2-Chloroadenosine, ATP, beta, gamma-methylene ATP (APPCP) and alpha, ...
journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
pub_type: 杂志文章
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abstract::To determine whether a mu opioid agonist modulates the effects of a kappa opioid agonist in squirrel monkeys responding under a shock titration procedure, morphine was administered in combination with an ED75 dose of U50,488. Morphine (0.03-0.3 mg/kg) did not alter U50,488's early peak effects (15-25 min post-injectio...
journal_title:European journal of pharmacology
pub_type: 杂志文章
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
pub_type: 杂志文章
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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journal_title:European journal of pharmacology
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