Abstract:
:Nitric oxide (NO) is produced by the enzyme nitric oxide synthase (NOS) and has been implicated in inter- and intracellular communication in the nervous system. The present study was undertaken to assess the effects of sodium nitroprusside (SNP) and hydroxylamine (HOA), NO donors, on a dopamine (DA)-induced K+ current in identified Aplysia neurons using voltage-clamp and pressure ejection techniques. Bath-applied SNP (10-25 microM) reduced the DA-induced K+ current without affecting the resting membrane conductance and holding current. The DA-induced K+ current also was inhibited by the focal application of 200 microM HOA to the neuron somata. The DA-induced K+ current suppressing effects of SNP and HOA are completely reversible. Pretreatment with 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 1 microM), a specific inhibitor of NO-stimulated guanylate cyclase, and hemoglobin (50 microM), a nitric oxide scavenger, decreased the SNP-induced inhibition of the DA-induced current. In contrast, intracellular injection of 1 mM guanosine 3',5'-cyclic monophosphate (cGMP) or bath-applied 3-isobutyl-1-methylxanthine (IBMX; 50 microM), a non-specific phosphodiesterase inhibitor, inhibited the DA-induced current, mimicking the effect of the NO donors. These results demonstrate that SNP and HOA inhibit the DA-induced K+ current and that the mechanism of NO inhibition of the DA-induced current involves cGMP-dependent protein kinase.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Sawada M,Ichinose M,Stefano GBdoi
10.1002/(SICI)1097-4547(19971101)50:3<450::AID-JNRsubject
Has Abstractpub_date
1997-11-01 00:00:00pages
450-6issue
3eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19971101)50:3<450::AID-JNRjournal_volume
50pub_type
杂志文章abstract::Neuron expression of the cytokine tumor necrosis factor-alpha (TNF), and the regulation of the levels of TNF by alpha(2)-adrenergic receptor activation were investigated. Adult rat hippocampal neurons and phorbol ester (PMA)-differentiated SH-SY5Y cells were examined. Intracellular levels of TNF mRNA accumulation, as ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10101
更新日期:2002-01-15 00:00:00
abstract::The large, medium-sized, and small neurons of the dorsal root ganglion (DRG) have different functions in the processing of various senses. Hyperpolarization-activated, cyclic nucleotide-gated channels (HCN) contribute greatly to neuronal excitability. In the present study, which used whole-cell patch clamp techniques ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20109
更新日期:2004-06-01 00:00:00
abstract::Relationships among different intensities of electroshock treatment (EST), EEG-recordable primary after discharge (PAD), and beta-adrenergic binding in various brain regions were investigated in adult rats. Different intensities of EST (1-2, 6-8, 20-30 mamp) were applied through ear-clip electrodes for 7 consecutive d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490070306
更新日期:1982-01-01 00:00:00
abstract::Cultured rat mesencephalic neurons were used to assess the effects of gamma-aminobutyric acid (GABA) transport blockers on toxicity caused by malonate, a reversible, competitive inhibitor of succinate dehydrogenase. Previous studies utilizing an ex vivo chick retinal preparation have shown that GABA release and cell s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1052
更新日期:2001-04-01 00:00:00
abstract::We have shown that ethanol exposure during embryogenesis affects a variety of parameters of neuronal growth. In this study we examined the direct effects of ethanol exposure on developing neuroblasts in culture. Neuroblast-enriched cultures derived from 3-day-old whole chick embryos were grown in the presence of ethan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300305
更新日期:1991-11-01 00:00:00
abstract::The inner ear of humans and experimental animals demonstrate an abundance of glucocorticoid receptors (GR). Glucocorticoids (GC) are widely used to treat different hearing disorders; yet the mechanisms of GC action on the inner ear are unknown. We demonstrate how GR can directly modulate hearing sensitivity in respons...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20795
更新日期:2006-05-01 00:00:00
abstract::This study tested the hypothesis that sensitivity to the Ca(2+) -induced loss of mitochondrial membrane potential (ΔΨ(m)) and the sensitivity of the loss of ΔΨ to mitochondrial permeability transition pore (PTP) inhibitors are different for neurons and astrocytes. Primary cultures of rat cortical neurons and astrocyte...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22709
更新日期:2011-12-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF-alpha) has attracted the greatest attention as a major factor in experimental autoimmune encephalomyelitis (EAE) pathogenesis. We compared rats undergoing EAE with manipulated but healthy animals by examining TNF-alpha gene expression in cells recovered from the brain. We used reverse ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960815)45:4<400::AID-JNR
更新日期:1996-08-15 00:00:00
abstract::The prenatal and early postnatal stages represent a critical time window for human brain development. Interestingly, this window partly overlaps with the maturation of the intestinal flora (microbiota) that play a critical role in the bidirectional communication between the central and the enteric nervous systems (mic...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24616
更新日期:2020-07-01 00:00:00
abstract::Neural stem cells can be propagated in culture as neurospheres, yielding neurons and glial cells upon differentiation. Although the neurosphere model is widely used, the functional properties of the neurosphere-derived neurons have been only partially characterized, and it is unclear whether repeated passaging alters ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20835
更新日期:2006-06-01 00:00:00
abstract::Cross-linking of alpha-synuclein and Lewy body formation have been implicated in the dopaminergic neuronal cell death observed in Parkinson's disease (PD); the mechanisms responsible, however, are not clear. Reactive oxygen species and advanced glycation end products (AGEs) have been found in the intracellular, alpha-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21644
更新日期:2008-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection in its human host often results in progressive dementia and encephalopathy in adults and children, respectively. The mechanisms underlying virus-induced neurocognitive dysfunction are not fully understood. However, several studies strongly suggest that secretory vi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20745
更新日期:2006-02-15 00:00:00
abstract::Although alterations in adenylate cyclase (AC) activity and somatostatin (SRIF) receptor density have been reported in Alzheimer's disease, the effects of amyloid beta-peptide (Abeta) on these parameters in the hippocampus are unknown. Our aim was to investigate whether the peptide fragment Abeta(25-35) can affect the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21115
更新日期:2007-02-01 00:00:00
abstract::New neurons are continuously generated throughout life in the subgranular zone in the dentate gyrus of the mammalian hippocampus and in the subventricular zone of the lateral ventricles. With the aid of new methodologies, significant progress has been made in the characterization of endogenous stem cells (ependymal ce...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23480
更新日期:2015-03-01 00:00:00
abstract::Contrary to widely held beliefs, the gene encoding proteolipid protein (PLP), the major structural protein of central nervous system myelin, is expressed in Schwann cells and their tumors. Proteolipid mRNA was identified in human acoustic neuromas and in rat and rabbit sciatic nerves using a human PLP cDNA as a probe....
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180402
更新日期:1987-01-01 00:00:00
abstract::The effect of two different potassium channel blockers, 4-aminopyridine (4-AP) and quinine, on the alpha 2-adrenoceptor mediated modulation of norepinephrine (NE) release was investigated. Pairs of mouse vasa deferentia were loaded with 3H-norepinephrine (3H-NE), superfused continuously, and stimulated electrically. 4...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200114
更新日期:1988-05-01 00:00:00
abstract::In this Mini-Review we present a new hypothesis in support of the neurodegenerative theory as a mechanism for the pathogenesis of multiple sclerosis (MS). The pathogenesis of MS results from changes in two distinct CNS compartments. These are the "myelin" and "nonmyelin" compartments. The myelin compartment is where p...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.20420
更新日期:2005-05-01 00:00:00
abstract::It was previously reported that a monoclonal antibody to beta-amyloid precursor protein (mab22C11; Boehringer Mannheim, Indianapolis, IN) labels an intermediate filament-associated protein (beta APP-IFAP) in cultured human skin fibroblasts (Dooley et al.: J Neurosci Res 33:60-67, 1992). The time course of its expressi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490380605
更新日期:1994-08-15 00:00:00
abstract::In a number of pathological states of the brain, the activation of the inducible nitric oxide synthase (iNOS) plays a major role. Interleukin (IL)-1beta is believed to be an essential factor in the induction of iNOS. However, little is known about the cascade of events culminating in iNOS expression in vivo. To identi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::To investigate the mechanism underlying the regulation of claudin-5, a tight junction protein that participates primarily in the constitution of the blood-brain barrier by bradykinin (BK), we established a primary culture of rat brain microvascular endothelial cells (BMECs). BMECs were treated with 10(-5) M BK, and ch...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23350
更新日期:2014-05-01 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) acutely modulates the efficacy of central glutamatergic synapses via activation of the receptor tyrosine kinase TrkB. On a longer time scale, recent evidence suggests an additional role of TrkB signaling in the formation of excitatory synaptic connections. Here, we have overexp...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10007
更新日期:2001-11-01 00:00:00
abstract::We describe here the presence of ubiquitin and its conjugation system in the rat retina. Retinal homogenates and supernatants conjugate [125I]human ubiquitin with either endogenous or exogenous proteins. The conjugating activity is relatively stable over time, requires ATP, and has a pH optimum of approximately 8. The...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300220
更新日期:1991-10-01 00:00:00
abstract::It has been postulated that cellular glutamate is released into the extracellular fluid when the energy supply of the brain is compromised (i.e., anoxia or oxygen/glucose deprivation), and there the amino acid triggers the so-called excitotoxic cascade, causing neuronal death. Several mechanisms for this release have ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20613
更新日期:2005-10-01 00:00:00
abstract::Pathogenesis of Parkinson's disease and related catecholaminergic neurological disorders is closely associated with changes in the levels of tyrosine hydroxylase (TH). Therefore, investigation of the regulation of the TH gene system should assist in understanding the pathomechanisms involved in these neurological diso...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23085
更新日期:2012-10-01 00:00:00
abstract::HAND2 (also known as dHAND) is a basic helix-loop-helix (bHLH) transcription factor essential for development of the heart, limbs, and neural crest-derived lineages. HAND2 expression is observed in a number of tissues derived from the neural crest, including components of the peripheral nervous system, where it has be...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20105
更新日期:2004-06-01 00:00:00
abstract::Soluble oligomeric aggregates of the amyloid-beta (A beta) peptide are believed to be the most neurotoxic A beta species affecting the brain in Alzheimer disease (AD), a terminal neurodegenerative disorder involving severe cognitive decline underscored by initial synaptic dysfunction and later extensive neuronal death...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22445
更新日期:2010-10-01 00:00:00
abstract::Acoustic overstimulation causes apoptotic cell death in the cochlea. This death process is mediated, in part, by the mitochondrial signaling pathway involving Bcl-2 family proteins. Myeloid cell leukemia sequence 1 (Mcl-l) is an antiapoptotic member of the Bcl-2 family. Its involvement in noise-induced hair cell death...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22333
更新日期:2010-06-01 00:00:00
abstract::The rationale for designing and synthesizing 24 different peptide analogs and subpeptides of residues 69-84 of guinea pig myelin basic protein is presented. The encephalitogenic potential of 19 of these peptides in Lewis rats is also given. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180130
更新日期:1987-01-01 00:00:00
abstract::Regulatory factor X4 variant 3 (RFX4_v3) is a recently identified transcription factor specifically expressed in the brain. Gene disruption in mice demonstrated that interruption of a single allele (heterozygous, +/-) prevented formation of the subcommissural organ (SCO), resulting in congenital hydrocephalus, whereas...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.21356
更新日期:2007-12-01 00:00:00
abstract::Synaptic circuitry in the upper dorsal horn, disorganized due to transganglionic degenerative atrophy (evoked by blockade of retrograde axoplasmic transport in the related peripheral nerve), begins to be reestablished by a process of regenerative synaptoneogenesis as soon as retrograde transport is resumed. Central ax...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220110
更新日期:1989-01-01 00:00:00