Multiple imprinted sense and antisense transcripts, differential methylation and tandem repeats in a putative imprinting control region upstream of mouse Igf2.

abstract：:Translational stop codon readthrough occurs in organisms ranging from viruses to mammals and is especially prevalent in decoding Drosophila and viral mRNAs. Recoding of UGA, UAG, or UAA to specify an amino acid allows a proportion of the protein encoded by a single gene to be C-terminally extended. The extended produc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hudson AM,Szabo NL,Loughran G,Wills NM,Atkins JF,Cooley L

更新日期：2021-02-02 00:00:00

abstract：:Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaika NV,Gebregiworgis T,Lewallen ME,Purohit V,Radhakrishnan P,Liu X,Zhang B,Mehla K,Brown RB,Caffrey T,Yu F,Johnson KR,Powers R,Hollingsworth MA,Singh PK

更新日期：2012-08-21 00:00:00

abstract：:We have determined the nucleotide sequence of the dihydrofolate reductase-thymidylate synthetase (DHFR-TS) gene of the protozoan parasite Leishmania major (dihydrofolate reductase, EC 1.5.1.3 and thymidylate synthase, EC 2.1.1.45). The DHFR-TS protein is encoded by a single 1560-base-pair open reading frame within gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beverley SM,Ellenberger TE,Cordingley JS

更新日期：1986-04-01 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disease of motor neurons. The inherited form of the disease, familial ALS, represents 5-10% of the total cases, and the best documented of these are due to lesions in SOD1, the gene encoding copper-zinc superoxide dismutase (CuZnSOD). The mechanism by w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valentine JS,Hart PJ

更新日期：2003-04-01 00:00:00

abstract：:The DNA-containing Epstein-Barr herpesvirus has been implicated in the etiology of Burkitt's lymphoma, a malignant tumor of children in Africa. Recently, however, particles possessing four biochemical characteristics of RNA tumor viruses have also been identified in these tumors. The fact that both types of viruses ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peters WP,Kufe D,Schlom J,Frankel JW,Prickett CO,Groupé V,Spiegelman S

更新日期：1973-11-01 00:00:00

abstract：:Induction, maintenance, and amplification of tumor-protective immunity after cytokine gene therapy is essential for the clinical success of immunotherapeutic approaches. We investigated whether this could be achieved by single-chain IL-12 (scIL-12) gene therapy followed by tumor-targeted IL-2 using a fusion protein co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lode HN,Xiang R,Duncan SR,Theofilopoulos AN,Gillies SD,Reisfeld RA

更新日期：1999-07-20 00:00:00

abstract：:The endometrial epithelium of the uterus regenerates periodically. The cellular source of newly regenerated endometrial epithelia during a mouse estrous cycle or a human menstrual cycle is presently unknown. Here, I have used single-cell lineage tracing in the whole mouse uterus to demonstrate that epithelial stem cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin S

更新日期：2019-04-02 00:00:00

abstract：:Gene expression is a stochastic, or "noisy," process. This noise comes about in two ways. The inherent stochasticity of biochemical processes such as transcription and translation generates "intrinsic" noise. In addition, fluctuations in the amounts or states of other cellular components lead indirectly to variation i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swain PS,Elowitz MB,Siggia ED

更新日期：2002-10-01 00:00:00

abstract：:B-1 B cells have been proposed to be preferentially generated from fetal progenitors, but this view is challenged by studies concluding that B-1 production is sustained throughout adult life. To address this controversy, we compared the efficiency with which hematopoietic stem cells (HSCs) and common lymphoid progenit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barber CL,Montecino-Rodriguez E,Dorshkind K

更新日期：2011-08-16 00:00:00

abstract：:Ion channels in excitable cells function in macromolecular complexes in which auxiliary proteins modulate the biophysical properties of the pore-forming subunits. Hyperpolarization-activated, cyclic nucleotide-sensitive HCN4 channels are critical determinants of membrane excitability in cells throughout the body, incl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peters CH,Myers ME,Juchno J,Haimbaugh C,Bichraoui H,Du Y,Bankston JR,Walker LA,Proenza C

更新日期：2020-07-28 00:00:00

abstract：:Emerging evidence suggests that tissue plasminogen activator (tPA), currently the only FDA-approved medication for ischemic stroke, exerts important biological actions on the CNS besides its well-known thrombolytic effect. In this study, we investigated the role of tPA on primary neurons in culture and on brain recove...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pu H,Shi Y,Zhang L,Lu Z,Ye Q,Leak RK,Xu F,Ma S,Mu H,Wei Z,Xu N,Xia Y,Hu X,Hitchens TK,Bennett MVL,Chen J

更新日期：2019-04-30 00:00:00

abstract：:In the presence of a single-stranded-DNA-binding protein (SSB), the elongation of primed DNA templates by DNA polymerase delta (pol delta) is dependent on ATP and two protein factors, activator 1 (A1) and proliferating cell nuclear antigen (PCNA). We have examined the interaction of these proteins with (dA)4500.(dT)12...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee SH,Hurwitz J

更新日期：1990-08-01 00:00:00

abstract：:We have detected an endoglycosidase activity produced by Flavobacterium meningosepticum. This enzyme, named endo F, cleaves glycans of both the high-mannose and the complex type linked through asparagine to the protein backbone. The data indicate that cleavage occurs via hydrolysis of the glycosidic bond of the N,N'-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elder JH,Alexander S

更新日期：1982-08-01 00:00:00

abstract：:It has long been recognized that a suite of proteins exists in coral skeletons that is critical for the oriented precipitation of calcium carbonate crystals, yet these proteins remain poorly characterized. Using liquid chromatography-tandem mass spectrometry analysis of proteins extracted from the cell-free skeleton o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drake JL,Mass T,Haramaty L,Zelzion E,Bhattacharya D,Falkowski PG

更新日期：2013-03-05 00:00:00

abstract：:Two strains that overproduce endonuclease III were found in a colony bank containing hybrid ColE1-Escherichia coli plasmids. The enzyme was identified in crude extracts by the degradation of partially depyrimidinated DNA in the presence of EDTA, by its sedimentation velocity, and by its associated thymine glycol-DNA g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cunningham RP,Weiss B

更新日期：1985-01-01 00:00:00

abstract：:The operon model was proposed by Jacob and Monod in 1961 to explain the regulation of enzyme synthesis in bacteria. The model requires two genetic elements: a locus i which directs the synthesis of a repressor, and a locus called the operon which contains an operator gene and associated structural genes. The repressor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zubay G,Lederman M

更新日期：1969-02-01 00:00:00

abstract：:Adenovirus (Ad) vectors have been extensively used to deliver recombinant genes to a great variety of cell types in vitro and in vivo. Ad-based vectors are available that replace the Ad early region 1 (E1) with recombinant foreign genes. The resultant E1-deleted vectors can then be propagated on 293 cells, a human emb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amalfitano A,Begy CR,Chamberlain JS

更新日期：1996-04-16 00:00:00

abstract：:The wide diversity of dendritic trees is one of the most striking features of neural circuits. Here we develop a general quantitative theory relating the total length of dendritic wiring to the number of branch points and synapses. We show that optimal wiring predicts a 2/3 power law between these measures. We demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuntz H,Mathy A,Häusser M

更新日期：2012-07-03 00:00:00

abstract：:We have shown previously that guanine nucleotide-binding protein (G protein) beta gamma complexes purified from bovine brain membranes are methyl esterified on a C-terminal cysteine residue of the gamma polypeptide. In the present study, 3H-methylated G beta gamma complexes cleaved to their constituent amino acids by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamane HK,Farnsworth CC,Xie HY,Howald W,Fung BK,Clarke S,Gelb MH,Glomset JA

更新日期：1990-08-01 00:00:00

abstract：:Sympathetic activation of β-adrenoreceptors (β-AR) represents a hallmark in the development of heart failure (HF). However, little is known about the underlying mechanisms of gene regulation. In human ventricular myocardium from patients with end-stage HF, we found high levels of phosphorylated histone 3 at serine-28 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saadatmand AR,Sramek V,Weber S,Finke D,Dewenter M,Sticht C,Gretz N,Wüstemann T,Hagenmueller M,Kuenzel SR,Meyer-Roxlau S,Kramer M,Sossalla S,Lehmann LH,Kämmerer S,Backs J,El-Armouche A

更新日期：2019-10-29 00:00:00

abstract：:Germ cells are required for the successful propagation of sexually reproducing species. Understanding the mechanisms by which these cells are specified and how their totipotency is established and maintained has important biomedical and evolutionary implications. Freshwater planarians serve as fascinating models for s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Zayas RM,Guo T,Newmark PA

更新日期：2007-04-03 00:00:00

abstract：:Domain walls, commonly occurring at the interface of different phases in solid-state materials, have recently been harnessed at the structural scale to enable additional modes of functionality. Here, we combine experimental, numerical, and theoretical tools to investigate the domain walls emerging upon uniaxial compre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deng B,Yu S,Forte AE,Tournat V,Bertoldi K

更新日期：2020-12-08 00:00:00

abstract：:The involvement of epigenetic alterations in mediating effects of estrogens on memory is unknown. The present study determined whether histone acetylation and DNA methylation are critical for the potent estrogen 17beta-estradiol (E(2)) to enhance object recognition memory. We show that dorsal hippocampal E(2) infusion...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Z,Fan L,Frick KM

更新日期：2010-03-23 00:00:00

abstract：:Usual human livers contain two major aldehyde dehydrogenase [(ALDH) aldehyde:NAD+ oxidoreductase] isozymes--i.e., a cytosolic ALDH1 component and a mitochondrial ALDH2 component--whereas approximately equal to 50% of Orientals are "atypical" and have only the ALDH1 isozyme and are missing the ALDH2 isozyme. We previou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshida A,Huang IY,Ikawa M

更新日期：1984-01-01 00:00:00

abstract：:Heat shock factors (HSFs) are the master regulators of transcription under protein-damaging conditions, acting in an environment where the overall transcription is silenced. We determined the genomewide transcriptional program that is rapidly provoked by HSF1 and HSF2 under acute stress in human cells. Our results rev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vihervaara A,Sergelius C,Vasara J,Blom MA,Elsing AN,Roos-Mattjus P,Sistonen L

更新日期：2013-09-03 00:00:00

abstract：:Human cells that suffer mild DNA damage can enter a reversible state of growth arrest known as quiescence. This decision to temporarily exit the cell cycle is essential to prevent the propagation of mutations, and most cancer cells harbor defects in the underlying control system. Here we present a mechanistic mathemat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heldt FS,Barr AR,Cooper S,Bakal C,Novák B

更新日期：2018-03-06 00:00:00

abstract：:Optimal flowering time is critical to the success of modern agriculture. Sorghum is a short-day tropical species that exhibits substantial photoperiod sensitivity and delayed flowering in long days. Genotypes with reduced photoperiod sensitivity enabled sorghum's utilization as a grain crop in temperate zones worldwid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy RL,Klein RR,Morishige DT,Brady JA,Rooney WL,Miller FR,Dugas DV,Klein PE,Mullet JE

更新日期：2011-09-27 00:00:00

abstract：:A genetic defect in a CC-chemokine receptor (CCR)-5, the principal coreceptor for the macrophage-tropic HIV type 1 (HIV-1), recently was found to naturally protect CCR-5-defective, but healthy, individuals from HIV-1 infection. In this study, we mimic the natural resistance of the CCR-5-defective individuals by design...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang AG,Bai X,Huang XF,Yao C,Chen S

更新日期：1997-10-14 00:00:00

abstract：:Cysteine site-directed mutagenesis was used to create variants of Escherichia coli ribosomal protein L7/L12 that have single cysteine substitutions, at residues 63 or 89, located in different exposed loops in the structure of the globular C-terminal domain indicated by the crystallographic structure. That structure sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oleinikov AV,Jokhadze GG,Traut RR

更新日期：1993-11-01 00:00:00

abstract：:A purified EcoRI restriction endonuclease fragment that determines resistance to kanamycin and is incapable of self-replication was used to select autonomously replicating fragments from an EcoRI digest of a Salmonella typhimurium F' plasmid containing the chromosomal region believed to include the S. typhimurium orig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zyskind JW,Deen LT,Smith DW

更新日期：1979-07-01 00:00:00