Abstract:
:The DNA-containing Epstein-Barr herpesvirus has been implicated in the etiology of Burkitt's lymphoma, a malignant tumor of children in Africa. Recently, however, particles possessing four biochemical characteristics of RNA tumor viruses have also been identified in these tumors. The fact that both types of viruses are found suggests that an interaction between them may be playing a role in the etiology of Burkitt's lymphoma. To explore this possibility with a defined animal model, experiments were performed with the avian Marek's disease, a malignant lymphoproliferative disease associated with a herpesvirus. Controlled contact studies demonstrated that tumorigenesis in a line of isolator-derived, barrier-sustained, specific pathogen-free chickens requires exposure to both the Marek's disease herpesvirus and an avian leukosis virus, Rous-associated virus, type 2. Exposure to either agent alone did not result in tumors. Molecular hybridization experiments to cytoplasmic RNA from similarly contact-exposed conventional Cornell S-line chickens provided further evidence for the occurrence of an interaction between Marek's disease herpesvirus and the avian leukosis virus.
journal_name
Proc Natl Acad Sci U S Aauthors
Peters WP,Kufe D,Schlom J,Frankel JW,Prickett CO,Groupé V,Spiegelman Sdoi
10.1073/pnas.70.11.3175subject
Has Abstractpub_date
1973-11-01 00:00:00pages
3175-8issue
11eissn
0027-8424issn
1091-6490journal_volume
70pub_type
杂志文章abstract::A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative frequency of colonies wit...
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