Isolation and mapping of plasmids containing the Salmonella typhimurium origin of DNA replication.

abstract：:There are defined medullary, mesencephalic, hypothalamic, and thalamic functions in regulation of respiration, but knowledge of cortical control and the elements subserving the consciousness of breathlessness and air hunger is limited. In nine young adults, air hunger was produced acutely by CO(2) inhalation. Comparis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brannan S,Liotti M,Egan G,Shade R,Madden L,Robillard R,Abplanalp B,Stofer K,Denton D,Fox PT

更新日期：2001-02-13 00:00:00

abstract：:A recently determined set of 20 NMR-derived conformations of a 48-residue all-alpha-helical protein, (PDB ID code 2JVD), is validated here by comparing the observed (13)C(alpha) chemical shifts with those computed at the density functional level of theory. In addition, a recently introduced physics-based method, aimed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vila JA,Aramini JM,Rossi P,Kuzin A,Su M,Seetharaman J,Xiao R,Tong L,Montelione GT,Scheraga HA

更新日期：2008-09-23 00:00:00

abstract：:Genetic variation in the peptide-binding groove of the highly polymorphic HLA class I molecules has repeatedly been associated with HIV-1 control and progression to AIDS, accounting for up to 12% of the variation in HIV-1 set point viral load (spVL). This suggests a key role in disease control for HLA presentation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arora J,McLaren PJ,Chaturvedi N,Carrington M,Fellay J,Lenz TL

更新日期：2019-01-15 00:00:00

abstract：:Mutations at position 187 in secreted gelsolin enable aberrant proteolysis at the 172-173 and 243-244 amide bonds, affording the 71-residue amyloidogenic peptide deposited in Familial Amyloidosis of Finnish Type (FAF). Thermodynamic comparisons of two different domain 2 constructs were carried out to study possible ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnaswamy G,Huff ME,Su AI,Rion S,Kelly JW

更新日期：2001-02-27 00:00:00

abstract：:Granulocytes generate a "respiratory burst" of NADPH oxidase-dependent superoxide anion (O(2)(-*)) production that is required for efficient clearance of bacterial pathogens. Hv1 mediates a voltage-gated H(+) channel activity that is proposed to serve a charge-balancing role in granulocytic phagocytes such as neutroph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramsey IS,Ruchti E,Kaczmarek JS,Clapham DE

更新日期：2009-05-05 00:00:00

abstract：:Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar KP,Akoulitchev S,Reinberg D

更新日期：1998-08-18 00:00:00

abstract：:Plant germ cells develop in specialized haploid structures, termed gametophytes. The female gametophyte patterns of flowering plants are diverse, with often unknown adaptive value. Here we present the Arabidopsis fiona mutant, which forms a female gametophyte that is structurally and functionally reminiscent of a phyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kägi C,Baumann N,Nielsen N,Stierhof YD,Gross-Hardt R

更新日期：2010-12-21 00:00:00

abstract：:The biological activity of the transcription factor NF-kappaB is differentially controlled by three IkappaB proteins, Ikappa Balpha, Ikappa Bbeta, and Ikappa Bepsilon. We have examined the molecular basis for the differential inhibitory strengths of IkappaB proteins by constructing hybrid IkappaBs and found that the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simeonidis S,Stauber D,Chen G,Hendrickson WA,Thanos D

更新日期：1999-01-05 00:00:00

abstract：:Complex social behavior in Microtus voles and other mammals has been postulated to be under the direct genetic control of a single locus: the arginine vasopressin 1a receptor (avpr1a) gene. Using a phylogenetic approach, we show that a repetitive element in the promoter region of avpr1a, which reportedly causes social...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fink S,Excoffier L,Heckel G

更新日期：2006-07-18 00:00:00

abstract：:Some monoclonal antibodies (mAbs) against interleukin (IL) 1 alpha have been found to activate antigen-presenting cells (APC, human peripheral blood monocytes and B lymphocytes), so that unstimulated T lymphocytes cultured with them are induced to proliferate and secrete IL-2. Control mAbs of the same isotypes and mAb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eugui EM,Almquist SJ

更新日期：1990-02-01 00:00:00

abstract：:Somatic cell hybrids have been prepared at high frequency between the rat muscle cell line L6 and human fetal muscle cells. The hybrid cells express several human gene products including an antigen, 12E7, controlled by the human X chromosome, Thy-1, and several human isoenzymes. In addition, one clone (37-11) expresse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quinn CA,Goodfellow PN,Povey S,Walsh FS

更新日期：1981-08-01 00:00:00

abstract：:Earlier papers demonstrated an extensive genetic exchange among fluorescent Pseudomonads; this one documents for genes specifying enzymes of peripheral dissimilation an extrachromosomal array, segregation, and frequent interstrain transfer. An hypothesis is presented of a general mechanism for the formation and mainte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rheinwald JG,Chakrabarty AM,Gunsalus IC

更新日期：1973-03-01 00:00:00

abstract：:This communication reports a modulating effect of intracellular ATP on the steady-state and kinetic properties of the delayed rectifier of the giant axon of the squid. When internally dialyzed or perfused giant axons from Loligo plei or Loligo pealei are voltage clamped at -60 mV and washed free of ATP, the potassium ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bezanilla F,Caputo C,DiPolo R,Rojas H

更新日期：1986-04-01 00:00:00

abstract：:Biological processes such as circadian rhythms, cell division, metabolism, and development occur as ordered sequences of events. The synchronization of these coordinated events is essential for proper cell function, and hence the determination of critical time points in biological processes is an important component o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramakrishnan N,Tadepalli S,Watson LT,Helm RF,Antoniotti M,Mishra B

更新日期：2010-07-13 00:00:00

abstract：:Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reyenolds JA,Stoeckenius W

更新日期：1977-07-01 00:00:00

abstract：:This article extends our previous quantitative analysis of the relationship between the dynamics of the primary structure of DNA and mutagenesis associated with single-strand lesions to an analysis of the production and processing of endogenous double-strand breaks (EDSBs) and to their implications for oncogenesis. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vilenchik MM,Knudson AG

更新日期：2003-10-28 00:00:00

abstract：:This work describes a multifunctional phage lambda expression vector system, lambda YES, designed to facilitate gene isolation from eukaryotes by complementation of Escherichia coli and Saccharomyces cerevisiae mutations. lambda YES vectors have a selection for cDNA inserts using an oligo adaptor strategy and are capa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elledge SJ,Mulligan JT,Ramer SW,Spottswood M,Davis RW

更新日期：1991-03-01 00:00:00

abstract：:The virulence of many pathogens depends upon their ability to cope with immune-generated nitric oxide (NO·). In Escherichia coli, the major NO· detoxification systems are Hmp, an NO· dioxygenase (NOD), and NorV, an NO· reductase (NOR). It is well established that Hmp is the dominant system under aerobic conditions, wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson JL,Brynildsen MP

更新日期：2016-03-22 00:00:00

abstract：:The ciliary epithelium in the eye consists of pigmented epithelial cells that express the α1β1 isoform of Na,K-ATPase and nonpigmented epithelial cells that express mainly the α2β3 isoform. In principle, a Na,K-ATPase inhibitor with selectivity for α2β3 that penetrates the cornea could effectively reduce intraocular p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katz A,Tal DM,Heller D,Habeck M,Ben Zeev E,Rabah B,Bar Kana Y,Marcovich AL,Karlish SJ

更新日期：2015-11-03 00:00:00

abstract：:A mitochondrial gene from Saccharomyces cerevisiae encoding a hydrophobic membrane protein, subunit 8 of the F0/F1-type mitochondrial ATPase complex, has been functionally replaced by an artificial nuclear gene specifying an imported version of this protein. The experiments reported here utilized a multicopy expressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagley P,Farrell LB,Gearing DP,Nero D,Meltzer S,Devenish RJ

更新日期：1988-04-01 00:00:00

abstract：:Molecular dynamics simulations of the response to oscillating electric field elicited from an altitudinal dipolar molecular rotor mounted on the Au(111) surface and previously studied experimentally in static fields show unidirectional rotation in one of the three pairs of conformational enantiomers. The simulations a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horinek D,Michl J

更新日期：2005-10-04 00:00:00

abstract：:Nogo isoforms (Nogo-A and -B) have been implicated in regulating neural and cardiovascular functions, such as cell spreading and chemotaxis. Unlike the loop domain (Nogo-66) found in all Nogo isoforms that can interact with a neural-specific Nogo-66 receptor, the receptor for the amino terminus of Nogo-B that mediates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miao RQ,Gao Y,Harrison KD,Prendergast J,Acevedo LM,Yu J,Hu F,Strittmatter SM,Sessa WC

更新日期：2006-07-18 00:00:00

abstract：:Down syndrome (DS) is the most common form of intellectual disability. The cognitive alterations in DS are thought to depend on brain regions critical for learning and memory such as the prefrontal cortex (PFC) and the hippocampus (HPC). Neuroimaging studies suggest that increased brain connectivity correlates with lo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alemany-González M,Gener T,Nebot P,Vilademunt M,Dierssen M,Puig MV

更新日期：2020-05-26 00:00:00

abstract：:Disruption of the nucleotide excision repair (NER) pathway by mutations can cause xeroderma pigmentosum, a syndrome predisposing affected individuals to development of skin cancer. The xeroderma pigmentosum C (XPC) protein is essential for initiating global genome NER by recognizing the DNA lesion and recruiting downs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ming M,Shea CR,Guo X,Li X,Soltani K,Han W,He YY

更新日期：2010-12-28 00:00:00

abstract：:Proper resolution of inflammation is vital for repair and restoration of homeostasis after tissue damage, and its dysregulation underlies various noncommunicable diseases, such as cardiovascular and metabolic diseases. Macrophages play diverse roles throughout initial inflammation, its resolution, and tissue repair. D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakayama Y,Fujiu K,Yuki R,Oishi Y,Morioka MS,Isagawa T,Matsuda J,Oshima T,Matsubara T,Sugita J,Kudo F,Kaneda A,Endo Y,Nakayama T,Nagai R,Komuro I,Manabe I

更新日期：2020-06-23 00:00:00

abstract：:The structure of the NH2-terminal, 20-residue membrane-bound portion of melittin has been computed with empirical energies (ECEPP, Empirical Conformational Energy Program for Peptides). First, a search was made for long stretches of nonpolar residues. Then, the low-energy conformations of these segments were built up ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus MR,Klausner RD,Scheraga HA

更新日期：1982-08-01 00:00:00

abstract：:Before fertilization, vertebrate eggs are arrested in meiosis II by cytostatic factor (CSF), which holds the anaphase-promoting complex (APC) in an inactive state. It was recently reported that Mos, an integral component of CSF, acts in part by promoting the Rsk-mediated phosphorylation of the APC inhibitor Emi2/Erp1....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu JQ,Hansen DV,Guo Y,Wang MZ,Tang W,Freel CD,Tung JJ,Jackson PK,Kornbluth S

更新日期：2007-10-16 00:00:00

abstract：:The "disease-specific" (dsp) region next to the hrp gene cluster of Erwinia amylovora is required for pathogenicity but not for elicitation of the hypersensitive reaction. A 6.6-kb apparent operon, dspEF, was found responsible for this phenotype. The operon contains genes dspE and dspF and is positively regulated by h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bogdanove AJ,Kim JF,Wei Z,Kolchinsky P,Charkowski AO,Conlin AK,Collmer A,Beer SV

更新日期：1998-02-03 00:00:00

abstract：:Despite its theoretical prominence and sound principles, integrated pest management (IPM) continues to suffer from anemic adoption rates in developing countries. To shed light on the reasons, we surveyed the opinions of a large and diverse pool of IPM professionals and practitioners from 96 countries by using structur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parsa S,Morse S,Bonifacio A,Chancellor TC,Condori B,Crespo-Pérez V,Hobbs SL,Kroschel J,Ba MN,Rebaudo F,Sherwood SG,Vanek SJ,Faye E,Herrera MA,Dangles O

更新日期：2014-03-11 00:00:00

abstract：:The understanding of mechanisms controlling zinc absorption and metabolism at the molecular level has advanced recently. Kinetics of zinc transport have been investigated for many years, but only recently have genes coding for proteins thought to be involved in the transport process been cloned. Four putative zinc tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMahon RJ,Cousins RJ

更新日期：1998-04-28 00:00:00