Abstract:
:We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100-500 bases are presently detected.
journal_name
Proc Natl Acad Sci U S Aauthors
Essevaz-Roulet B,Bockelmann U,Heslot Fdoi
10.1073/pnas.94.22.11935subject
Has Abstractpub_date
1997-10-28 00:00:00pages
11935-40issue
22eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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更新日期:2003-12-09 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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