Abstract:
:The in vivo state of assembly of myosin in vertebrate smooth muscle is controversial. In vitro studies on purified smooth muscle myosin show that it is monomeric (10S) under relaxing conditions and filamentous under contraction conditions. Electron microscopic and antibody labelling studies of intact smooth muscles, on the other hand, suggest that myosin is filamentous in the relaxed as well as the contracting state and that 10S myosin occurs only in trace amounts. However, birefringence, conventional EM and X-ray diffraction evidence suggests that in certain smooth muscles in vivo (e.g. rat anococcygeus), while myosin filaments exist in the relaxed state, their number increases on contraction. Here, we have used low temperature electron microscopic techniques (rapid freezing followed by freeze-substitution), which preserve labile components in close to their in vivo state, to detect any change in filament number on contraction. The results from rat anococcygeus have been compared with those from guinea pig taenia coli, in which other techniques have revealed no change in filament number. In the anococcygeus, we find evidence for a 23% increase in filament density in transverse sections of contracting muscle compared with relaxed muscle. In the taenia coli we find no change. These results are in qualitative agreement with earlier findings. They provide evidence for polymerization of myosin in contracting rat anococcygeus, and suggest that this process is subtle and occurs only in some smooth muscles.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Xu JQ,Gillis JM,Craig Rdoi
10.1023/a:1018634412117subject
Has Abstractpub_date
1997-06-01 00:00:00pages
381-93issue
3eissn
0142-4319issn
1573-2657journal_volume
18pub_type
杂志文章abstract::Striated muscle fine structure began to be really understood following a comprehensive survey of the matter carried out by William Bowman in the late 1830s. The publications resulting from such a study, the first of which earned for the author a precocious election as Fellow of the Royal Society, are herewith examined...
journal_title:Journal of muscle research and cell motility
pub_type: 传,历史文章,杂志文章
doi:10.1007/s10974-005-9048-z
更新日期:2006-01-01 00:00:00
abstract::The β-adrenergic receptor stimulation improves endurance in fast twitch muscles and these effects are sensitive to extracellular Ca2+ influx. Present study is aimed to determine the effects of adrenaline, with different concentrations of extracellular Ca2+[Formula: see text], on the contractility and endurance of slow...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-019-09551-9
更新日期:2019-12-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-011-9281-6
更新日期:2012-05-01 00:00:00
abstract::The force-velocity (T-V) relation and the force-extension (T1) relation from single fibres isolated from the muscle tibialis anterior of the frog were determined at present times during the rise of tension and the plateau of an isometric tetanus. During the rise of an isometric tetanus the value of V0 (the velocity of...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00712029
更新日期:1983-04-01 00:00:00
abstract::A high resolution, time efficient method is described for determining the complex modulus of activated striated muscle. In this method the applied oscillation of muscle length takes the form of pseudo-random binary noise, PRBN. As a time-domain signal, PRBN is an extension of the double step approach to include n step...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01753650
更新日期:1986-08-01 00:00:00
abstract::Several mutations in the beta-myosin heavy chain gene cause hypertrophic cardiomyopathy. This study investigates (1) the in vitro velocities of translocation of fluorescently-labelled actin by beta-myosin purified from soleus muscle of 30 hypertrophic cardiomyopathy patients with seven distinct beta-myosin heavy chain...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1018613907574
更新日期:1997-06-01 00:00:00
abstract::This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation-contraction (E-C) coupling. In skinned EDL fibres of the rat, force responses to depola...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1012289526618
更新日期:2001-01-01 00:00:00
abstract::Guinea-pig soleus muscles were denervated and electrically stimulated for periods of 43 to 66 days. Stimuli were in 1 s bursts of 40 Hz pulses, repeated every 5 min. Other guinea-pigs were denervated for 82 days without stimulation and, in a third group, the soleus muscle was necrotized and allowed to regenerate witho...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1018619503456
更新日期:1997-10-01 00:00:00
abstract::The trimeric protein AMP-activated protein kinase (AMPK) is an important sensor of energetic status and cellular stress, and mutations in genes encoding two of the regulatory γ subunits cause inherited disorders of either cardiac or skeletal muscle. AMPKγ2 mutations cause hypertrophic cardiomyopathy with glycogen depo...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-013-9359-4
更新日期:2013-12-01 00:00:00
abstract::The change in orientation of myosin crossbridges in contracting muscle during sudden length changes was examined by fluorescence polarization. This study used a fluorescent ATP analogue, 1,N6-etheno-2-aza-ATP(epsilon-2-aza-ATP) as a probe. Its fluorescence is considerably enhanced upon binding with myosin and is depen...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00712310
更新日期:1985-02-01 00:00:00
abstract::Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00141557
更新日期:1993-12-01 00:00:00
abstract::Polyclonal antibodies have been developed against the junctional feet or spanning protein from skeletal muscle triads. These probes in combination with immunogold labels have been used to localize the spanning protein by electron microscope of isolated vesicles from terminal cisternae/triads. The spanning protein anti...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01773877
更新日期:1988-08-01 00:00:00
abstract::MagFluo-4 fluorescence (Ca2+) transients associated with action potentials were measured in intact muscle fibres, manually dissected from toads ( Leptodactylus insularis ) or enzymatically dissociated from mice. In toads, the decay phase of the Ca2+ transients is described by a single exponential with a time constant ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-004-4071-z
更新日期:2004-01-01 00:00:00
abstract::The effects of two amino acids, arginine which has a positively charged side-chain and glutamate which has a negatively charged side-chain on the Ca2+-activation properties of the contractile apparatus were examined in four structurally and functionally different types of skeletal muscle; long- and short-sarcomere fib...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-004-2769-6
更新日期:2004-01-01 00:00:00
abstract::Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which p...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005356511634
更新日期:1998-02-01 00:00:00
abstract::Muscle development involves a complex sequence of time and spatially regulated cellular events leading to the formation of highly specialised syncytial muscle cells displaying a common feature, the capacity of contraction. Analyses of mechanisms controlling muscle development reveals that the main steps of muscle form...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-008-9133-1
更新日期:2007-01-01 00:00:00
abstract::The effects of a ramp stretch (amplitude <6% muscle fibre length (L0), speed < 13L0 s(-1)) on twitch tension and twitch tension re-development were examined in intact mammalian (rat) fast and slow muscle fibre bundles. The experiments were done in vitro at 20 degrees C and at an initial sarcomere length of 2.68 microm...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1010556623905
更新日期:2001-01-01 00:00:00
abstract::Two single mutations, R694N and E45Q, were introduced in the beta isoform of human cardiac myosin to remove permanent salt bridges E45:R694 and E98:R694 in the SH1-SH2 helix of the myosin head. Beta isoform-specific bridges E45:R694 and E98:R694 were discovered in the molecular dynamics simulations of the alpha and be...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-020-09588-1
更新日期:2020-09-14 00:00:00
abstract::Protein kinase C (PKC) is known to play important roles in integrin mediated cell spreading. This study investigated the role of PKC during insulin mediated muscle cell spreading, which was independent of integrin alpha5. We found that PKC-alpha becomes active and localise to membrane during insulin mediated cell spre...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1022028808806
更新日期:2002-01-01 00:00:00
abstract::Skeletal muscle fibers exhibit plasticity of their physiological and biochemical properties in response to the firing pattern from the innervating motor neuron. In particular, the gene expression pattern generally characteristic of a slow twitch fiber can be induced in a fast twitch fiber by chronic slow fiber type el...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-006-9080-7
更新日期:2006-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-011-9260-y
更新日期:2011-11-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1016369300532
更新日期:2001-01-01 00:00:00
abstract::We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00130421
更新日期:1994-04-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01739967
更新日期:1989-04-01 00:00:00
abstract::Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01774035
更新日期:1991-04-01 00:00:00
abstract::In isolated myosin the reaction sequence of essential thiol groups with N-ethylmaleimide was studied using the following five approaches: kinetics of the modification reaction, effects of modification on enzyme properties, affinity chromatography of isolated subfragment-1 stemming from modified myosin, isolation of cy...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711923
更新日期:1980-03-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-013-9336-y
更新日期:2013-05-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005489319058
更新日期:1999-02-01 00:00:00
abstract::The aim of the present study was to examine the temperature dependence of active force in lengthening and shortening muscle. Experiments were done, in vitro, on bundles of intact fibres (fibre length L(0) ~2 mm; sarcomere length ~2.5 μm) isolated from a rat fast muscle (flexor hallucis brevis) and a ramp length change...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-012-9307-8
更新日期:2012-10-01 00:00:00
abstract::The characteristics of the titin filament in the vicinity of the Z-line were investigated using immunoelectron microscopy. We used monoclonal titin antibodies T-11 and T-12 on single fibres of frog skeletal muscle, and on Z-line-extracted fibres. It is well established that the I-band region of titin is elastic. We fi...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00121293
更新日期:1993-08-01 00:00:00