Abstract:
INTRODUCTION:For its transparency, avascularity and possibility of measurement of the new vessels ingrowth, cornea is a frequent model for angiogenesis research. New vessels are made in several phases: after the action of the stimulus, there is a localised fragmentation of the basal membrane and the extracellular matrix around the involved capillary. Then, endothelial cells migrate through the openings in the capillary wall and the lysis of the extracellular matrix continues as a new vessel is being formed. Enzymes, plasminogen-activators, convert plasminogen from the blood and tissues to plasmin which starts proteolytic cascade of the lysis of the extracellular matrix. Amilorid, previously known as a diuretic, is found to be a competitive inhibitor of plasminogen activator-urokinase (u-PA). Its effect was shown in the prostaglandin-induced model of corneal vascularization [6]. PURPOSE:The purpose of this work is to show that amilorid acts as an angiostatic in the traumatic model of corneal vascularization. MATERIALS AND METHODS:There were two groups of experimental animals (rabbits, weight 1.5-3 kg). Deepitelisation and trephination were performed on all corneas (traumatic model of vascularisation). The first group of animals (17 eyes) was given 15 mg of amilorid (Sigma) intraperitoneally for five days, while the control group consisted of 7 eyes. Neovascularization was measured after 5 and 15 days. Animals were sacrificed on day 15. Three eyes from each group were tested for corneal wound tensile strength. RESULTS:In the experimental group there was a 0.5 mm ingrowth of the new vessels after the first five days and no further ingrowth till the end of the experiment. In the control group there was a 2 mm ingrowth after five days, and a further 1 mm after the next ten days. No statistical difference was found with regard to tensile strengths of the corneal wounds between the two groups. DISCUSSION:Although the traumatic model is closer to the clinical situation than the prostaglandin-induced vascularization model, more potential angiogenic factors are involved. Trephination damages basal membrane which may liberate angiogenic substances (heparan-sulphate, for example) [9]. Products of damaged cells attract leucocytes by haemotaxis. Also, hyopxia in the depth of the wound may be the site of macrophage induced angiogenesis [10]. It is difficult to say at which point amilorid acts but, considering the new vessel ingrowth for only 0.5 mm, it might be at the beginning of the process (lysis of extracellular matrix). CONCLUSION:Amilorid acts as angiogenesis inhibitor in the traumatic corneal vascularization model without changing the tensile strength of the wound.
journal_name
Srp Arh Celok Lekjournal_title
Srpski arhiv za celokupno lekarstvoauthors
Ignjatović Z,Nikolić Ljsubject
Has Abstractpub_date
1996-05-01 00:00:00pages
120-3issue
5-6eissn
0370-8179issn
2406-0895journal_volume
124pub_type
杂志文章abstract::There is a variety of terms related to the classification of voice disorders still in use. The basic characteristics are discussed. Classification according to Perello, modified by the author, is recommended. On the basis of this classification, the "basic diagnosis" is suggested, with all necessary diagnostic procedu...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,评审
doi:
更新日期:1995-05-01 00:00:00
abstract::Lipid and lipoprotein disorders are well known risk factors for atherosclerosis and its complications. The level of atherogenic LDL-cholesterol (LDL-C) is directly related to an increased risk of occurrence and progression of ischemic heart disease. Epidemiological and clinical studies have shown that the use of stati...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,评审
doi:10.2298/sarh1302104s
更新日期:2013-01-01 00:00:00
abstract::Clinical manifestation of chronic rhinitis is due to local release of mediators from inflammatory cells. Eosinophil leukocytes are important in pathogenesis of nasal hypersensitivity as well as nasal hyperreactivity [1, 2]. The aim of the study was to follow-up the effect of local corticosteroid treatment on a number ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 临床试验,杂志文章
doi:10.2298/sarh0208243u
更新日期:2002-07-01 00:00:00
abstract::Interest in measuring the quality of life (QL) in relation to health care has increased enormously in recent years. This is also true for end-stage renal failure where it is important not only to provide a better survival but also the quality of that survival. The aim of this study was to assess the relative influence...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Low-grade astrocytomas comprise a group of primary brain neoplasms with relatively low anaplastic potential, although through time they tend to behave more aggressively. They have a very heterogeneous natural course and clinical behavior. This report presents a natural history of a patient with low grade astrocytoma. ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0612537r
更新日期:2006-11-01 00:00:00
abstract:INTRODUCTION:Gastroesophageal reflux is a frequent disease which has a significant influence on the development of dental erosions. OBJECTIVE:The aim of this research was to determine the frequency of dental erosions among the patients with gastroesophageal reflux, as well as to verify the most common symptoms of gast...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1006292s
更新日期:2010-05-01 00:00:00
abstract:INTRODUCTION:Prevalence of sensorineural hearing loss is 1-3 per 1,000 newborns. Transient evoked otoacoustic emission (TEOAE) and automated auditory brain stem responses (AABR) are most frequently used methods in newborn hearing screening programmes. OBJECTIVE:The aim of this study was to examine hearing function in ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0706264b
更新日期:2007-05-01 00:00:00
abstract:INTRODUCTION:Family planning is an important aspect of population policy at the state level, because the demographic trends in Serbia are very unfavorable. OBJECTIVE:The objective of this study was to examine the differences in family planning between the women in rural and urban areas of Serbia. METHODS:This study r...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1312794a
更新日期:2013-11-01 00:00:00
abstract::The roots of hospital foundation in Serbs date from the 12th century, when the hospitals in the monasteries Hilandar (1199) and Studenica (1207) were established. The "Town" Hospital of Belgrade was founded in 1841, which had the status of town and regional hospital until 1881. After that, it was transformed into a ge...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 历史文章,杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:Previous studies suggested that effects of the surfactant administration in preterm intants with respiratory distress syndrome cannot be followed by lung ultrasound (L-US). OBJECTIVE:The aim of the paper is to evaluate the surfactant replacement therapy effects using a new, proposed grading system for L-U...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1512669l
更新日期:2015-11-01 00:00:00
abstract:INTRODUCTION:Predictable endodontic treatment depends on the dentist's knowledge about root canal morphology and its possible anatomic variations. The majority of mandibular canines have one root and root canal, but 15% may have two canals and a smaller number may have two distinct roots. The following clinical reports...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1410592s
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:The paper presents important facts in the application of recombinant human factor VIIa (rFVIIa) and in the treatment of renal cell carcer. CASE REPORT:A 69-year old male with infiltrative renal cell carcer underwent radical nephrectomy using Hasagawa's approach. The extirpated tumor was 35 cm in diameter....
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh08s3259j
更新日期:2008-09-01 00:00:00
abstract::The authors determined C3 and C4 complements, factor B properdin and immunoglobulins G, M and A in 20 healthy pregnant patients and in 20 patients with EPH gestosis. The analysis was performed in mothers sera, umbilical sera and amniotic fluid, and properdin factor B and IgG in urine. They used the nonfellometric tech...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract:INTRODUCTION:Successful riddle solving requires recognition of the meaning of words, attention, concentration, memory, connectivity and analysis of riddle content, and sufficiently developed associative thinking. OBJECTIVE:The aim of the study was to determine the ability to solve riddles in stroke patients who do or ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:2016-03-01 00:00:00
abstract:INTRODUCTION:In 1993 the gene responsible for Huntington's disease (IT15) was isolated [5]. It was mapped to the tip of the short arm of chromosome 4 and within its coding sequence, near the 5' end, it contained a certain number of trinicleotide (CAG)n (cytosine-adenine-guanine) repeats (Figure 1). This gene codes for ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract:INTRODUCTION:The American otolaryngologist Eagle was the first to describe styloid syndrome in 1937 and the syndrome was named after him (Eagle's syndrome). The original description of two separate syndromes is connected with his name: classical syndrome, which almost constantly occurs after tonsillectomy and carotid a...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0812650p
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:Nurse/patient relationship as a complex interrelation or as an interaction of the factor patient and factor nurse has been a subject of a number of studies during the past ten years. Nurse/patient communication is a special entity, usually observed within a framework of the wider nurse/patient relationship...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1002079v
更新日期:2010-01-01 00:00:00
abstract::It has been suggested that in the genesis of isoprenaline-induced myocardial necrosis in rats, along with the intracellular calcium homeostasis and energy production breakdown, the mechanism of cytotoxic oxygen free radical generation is also included. The effects of dimethylsulphoxide, a hydroxyl radical scavenger, o...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::Professional management of paediatric diabetology, according to consensus guidelines, involves the screening of microvascular complications at puberty. The subclinical form of peripheral neural dysfunction in diabetic teenagers is reported with a frequency of 50-88%, by different authors. The purpose of this study was...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0508358s
更新日期:2005-07-01 00:00:00
abstract::The authors present results of systematic use of percutaneous renal biopsy in children in the first three years of practice. From 1986 to 1989, 153 renal biopsies were done in 144 children and adolescents (75 males, 69 females) aged 4 months to 18 years. The most common indications were as follow: primary nephrotic sy...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::Diagnostics in patients with the acute coronary syndromes (ACS) is becoming even more complex with the increase of number of available options. In addition, the number of treatment options accessible to patients with ACS keeps on growing rapidly. Physicians must now choose from a wide variety of antiplatelet and antit...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,评审
doi:
更新日期:2006-03-01 00:00:00
abstract:INTRODUCTION:Experience with lamivudine treatment for acute severe hepatitis B is limited. Fulminant hepatitis develops in 1% of immunocompetent patients with acute hepatitis B. OBJECTIVE:The aim of this study was to evaluate the efficacy of lamivudine in the treatment of severe acute hepatitis B (SAHB) in immunocompe...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1412703v
更新日期:2014-11-01 00:00:00
abstract::Type and technique of anesthesia have an important effect on per operative surgical course. The aim of the study was prospective analyses of advantages of combined spinal, epidural and general anesthesia (CSEGA) versus general anesthesia (GA) in abdominal surgery according to: 1. operative course (haemodynamic stabili...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2298/sarh0306232m
更新日期:2003-05-01 00:00:00
abstract::Chronic renal failure (CRF) is almost always associated with high arterial blood pressure. Adequate control of hypertension slows down the progression of the disease, Inhibitors of angiotenzin-converting enzyme (ACE inhibitors) have proved to be very efficacious in decreasing high blood pressure. The aim of this study...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0204087t
更新日期:2002-03-01 00:00:00
abstract::The frequency and the degree of bronchial hyperreactivity was investigated in 92 patients suffering from chronic obstructive bronchitis. Bronchial reactivity was examgned by the inhalator acetylcholine test, with the use of a 5 mg dose of acetylcholine, diluted in 1 ml of sodium-chloride solution. The pulmonary functi...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract:Introduction:The anterior and middle superior alveolar nerve block was claimed to be unpredictably efficient for clinical application. Objective:The aim of this report was to establish the efficacy of the anterior and middle superior alveolar nerve block, applied with a computer-controlled injection system or a conven...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2016-09-01 00:00:00
abstract:INTRODUCTION:The triple therapy which consists of one of the protease inhibitor plus pegylated interferon and ribavirin (P/R) is the standard of care for the treatment of chronic hepatitis C virus (HCV) genotype 1 (G1) infection both in treatment-naïve and experienced patients. OBJECTIVE:The aim of this study was to a...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:2015-01-01 00:00:00
abstract::Although the incidence of prosthetic infection is low (1%-6%), the consequences (limb loss or death) are dramatic for a patient, with high mortality rate (25%-75%) and limb loss in 40%-75% of cases. In case of Szilagyi's grade III infection, standard procedure consists of the excision of prosthesis and wound debrideme...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0502069n
更新日期:2005-01-01 00:00:00
abstract:INTRODUCTION:Considering operative treatment of various pathological conditions and traumatic injuries of extremities latissimus dorsi flap presents the most frequently used flap in reconstructive surgery. OBJECTIVE:The aim of this paper was to analyze anatomical characteristics of the vascular pedicle of the latissim...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1008449i
更新日期:2010-07-01 00:00:00
abstract:UNLABELLED:Magnetic resonance imaging (MRI) has become the preferred imaging technique in multiple sclerosis (MS). Areas of increased signal (AIS) are detected on T2-weighted (T2W) pulse sequencies in 70-100% of patients with clinically definite MS. However, AIS similar to those seen in MS have also been described in h...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00