The 72nd codon change of p53 in primary renal cell carcinoma was confirmed as a polymorphism among Japanese.

Abstract:

OBJECTIVE:The role of p53 mutation in renal cell carcinoma (RCC) is not fully understood particularly among Japanese, although frequent loss of heterozygosity (LOH) has been demonstrated at polymorphic exon 4. METHODS:We examined the p53 gene in 43 primary RCC samples by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis for exons 4-9 and restriction fragment length polymorphism (RFLP) analysis. RESULTS:In PCR-SSCP analysis, no apparent mobility shift was observed regardless of the clinical stage of the disease, histological subtype or malignancy grade of the tumor. In RFLP analysis, none of 13 informative cases showed gross alteration of the gene. CONCLUSION:We conclude that mutations of the p53 gene are not major events in the development and progression of RCC among Japanese.

journal_name

Eur Urol

journal_title

European urology

authors

Kageyama Y,Yamamura Y,Oshima H,Ikawa Y

doi

10.1159/000474423

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

81-5

issue

1

eissn

0302-2838

issn

1873-7560

journal_volume

31

pub_type

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