Abstract:
:The intranasal route has been shown to be effective for immunization. However, immunization via this route may require the use of potent and safe adjuvant. The construction of non-toxic mutants of heat labile enterotoxin of Escherichia coli (LT), which is a potent mucosal adjuvant, is a major breakthrough for the development of mucosal vaccines. In this study we have assessed the ability of an LT mutant (LTK63) to act as an adjuvant following intranasal co-immunization with a peptide corresponding to a measles virus cytotoxic T lymphocyte (CTL) epitope. LTK63 was more effective at potentiating the in vivo induction of peptide-specific and measles virus-specific CTL responses than was administration of the peptide in saline. A concentration of 10 micrograms/dose of LTK63 was found to be the most effective in potentiating the in vivo priming of peptide-specific and measles virus-specific CTL responses. These findings highlight the potential of the non-toxic mutant of LT as a safe mucosal adjuvant for use in humans.
journal_name
Immunologyjournal_title
Immunologyauthors
Partidos CD,Pizza M,Rappuoli R,Steward MWdoi
10.1046/j.1365-2567.1996.d01-790.xsubject
Has Abstractpub_date
1996-12-01 00:00:00pages
483-7issue
4eissn
0019-2805issn
1365-2567journal_volume
89pub_type
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