当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > A eukaryotic enzyme that can disjoin dead-end covalent complexes between DNA and type I topoisomerases.

A eukaryotic enzyme that can disjoin dead-end covalent complexes between DNA and type I topoisomerases.

Abstract：

:The covalent joining of topoisomerases to DNA is normally a transient step in the reaction cycle of these important enzymes. However, under a variety of circumstances, the covalent complex is converted to a long-lived or dead-end product that can result in chromosome breakage and cell death. We have discovered and partially purified an enzyme that specifically cleaves the chemical bond that joins the active site tyrosine of topoisomerases to the 3' end of DNA. The reaction products made by the purified enzyme on a variety of model substrates indicate that the enzyme cleanly hydrolyzes the tyrosine-DNA phosphodiester linkage, thereby liberating a DNA terminated with a 3' phosphate. The wide distribution of this phosphodiesterase in eukaryotes and its specificity for tyrosine linked to the 3' end but not the 5' end of DNA suggest that it plays a role in the repair of DNA trapped in complexes involving eukaryotic topoisomerase I.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Yang SW,Burgin AB Jr,Huizenga BN,Robertson CA,Yao KC,Nash HA

doi

10.1073/pnas.93.21.11534

subject

Has Abstract

pub_date

1996-10-15 00:00:00

pages

11534-9

issue

21

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Severe iron deficiency anemia in transgenic mice expressing liver hepcidin.

    abstract：:We recently reported the hemochromatosis-like phenotype observed in our Usf2 knockout mice. In these mice, as in murine models of hemochromatosis and patients with hereditary hemochromatosis, iron accumulates in parenchymal cells (in particular, liver and pancreas), whereas the reticuloendothelial system is spared fro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.072632499

    authors： Nicolas G,Bennoun M,Porteu A,Mativet S,Beaumont C,Grandchamp B,Sirito M,Sawadogo M,Kahn A,Vaulont S

    更新日期：2002-04-02 00:00:00

  • Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport.

    abstract：:The Belgrade (b) rat has an autosomal recessively inherited, microcytic, hypochromic anemia associated with abnormal reticulocyte iron uptake and gastrointestinal iron absorption. The b reticulocyte defect appears to be failure of iron transport out of endosomes within the transferrin cycle. Aspects of this phenotype ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.3.1148

    authors： Fleming MD,Romano MA,Su MA,Garrick LM,Garrick MD,Andrews NC

    更新日期：1998-02-03 00:00:00

  • Resting GABA concentration predicts peak gamma frequency and fMRI amplitude in response to visual stimulation in humans.

    abstract：:Functional imaging of the human brain is an increasingly important technique for clinical and cognitive neuroscience research, with functional MRI (fMRI) of the blood oxygen level-dependent (BOLD) response and electroencephalography or magnetoencephalography (MEG) recordings of neural oscillations being 2 of the most ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0900728106

    authors： Muthukumaraswamy SD,Edden RA,Jones DK,Swettenham JB,Singh KD

    更新日期：2009-05-19 00:00:00

  • An evolutionary constraint: strongly disfavored class of change in DNA sequence during divergence of cis-regulatory modules.

    abstract：:The DNA of functional cis-regulatory modules displays extensive sequence conservation in comparisons of genomes from modestly distant species. Patches of sequence that are several hundred base pairs in length within these modules are often seen to be 80-95% identical, although the flanking sequence cannot even be alig...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505291102

    authors： Cameron RA,Chow SH,Berney K,Chiu TY,Yuan QA,Krämer A,Helguero A,Ransick A,Yun M,Davidson EH

    更新日期：2005-08-16 00:00:00

  • On the mechanism of action of ribonucleases: dinucleotide cleavage catalyzed by imidazole and Zn2+.

    abstract：:Cyclization/cleavage of the 2-(p-nitrophenyl) phosphate ester of propylene glycol is catalyzed by imidazole and, much more effectively, by Zn2+ with imidazole. In the latter case, the mechanism involves simultaneous Lewis acid/base catalysis. Similar Zn2+ and imidazole catalysis of cyclization/cleavage is seen with th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.6.1746

    authors： Breslow R,Huang DL,Anslyn E

    更新日期：1989-03-01 00:00:00

  • A functional examination of hapten-binding derivatives from a murine myeloma protein with immunoglobulin features.

    abstract：:Various enzymatic derivatives of the murine myeloma protein IgA(MOPC-315) were subjected to hapten-binding quantitation and circular dichroic analysis in an attempt to ascertain the structural localization and functional definition of the immunoglobulin active site. The extrinsic Cotton effects observed with near satu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.5.1940

    authors： Merz DC,Litman GW,Good RA

    更新日期：1974-05-01 00:00:00

  • Humans incorporate attention-dependent uncertainty into perceptual decisions and confidence.

    abstract：:Perceptual decisions are better when they take uncertainty into account. Uncertainty arises not only from the properties of sensory input but also from cognitive sources, such as different levels of attention. However, it is unknown whether humans appropriately adjust for such cognitive sources of uncertainty during p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1717720115

    authors： Denison RN,Adler WT,Carrasco M,Ma WJ

    更新日期：2018-10-23 00:00:00

  • Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha.

    abstract：:Adaptation to hypoxia is mediated by transactivation of hypoxia-responsive genes by hypoxia-inducible factor-1 (HIF-1) in complex with the CBP and p300 transcriptional coactivators. We report the solution structure of the cysteine/histidine-rich 1 (CH1) domain of p300 bound to the C-terminal transactivation domain of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.082117899

    authors： Freedman SJ,Sun ZY,Poy F,Kung AL,Livingston DM,Wagner G,Eck MJ

    更新日期：2002-04-16 00:00:00

  • Genotypic analysis of N-ethyl-N-nitrosourea-induced mutations by Taq I restriction fragment length polymorphism/polymerase chain reaction in the c-H-ras1 gene.

    abstract：:In genotypic mutation analysis DNA sequence changes are determined without the in vivo or in vitro selection of phenotypically altered cells. We have studied the induction of base-pair changes by N-ethyl-N-nitrosourea in Taq I endonuclease recognition site 2508-2511 (TCGA) of the c-H-ras1 gene in human fibroblasts by ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.12.5331

    authors： Chiocca SM,Sandy MS,Cerutti PA

    更新日期：1992-06-15 00:00:00

  • Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.

    abstract：:The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polype...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.13.4700

    authors： Kawakami K,Scheidereit C,Roeder RG

    更新日期：1988-07-01 00:00:00

  • Erratic overdispersion of three molecular clocks: GPDH, SOD, and XDH.

    abstract：:The neutrality theory predicts that the rate of neutral molecular evolution is constant over time, and thus that there is a molecular clock for timing evolutionary events. It has been observed that the variance of the rate of evolution is generally larger than expected according to the neutrality theory, which has rai...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.201392198

    authors： Rodríguez-Trelles F,Tarrío R,Ayala FJ

    更新日期：2001-09-25 00:00:00

  • The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias.

    abstract：:Genomic and cDNA clones for the human nerve growth factor receptor have been used in conjunction with somatic cell hybrid analysis and in situ hybridization to localize the nerve growth factor receptor locus to human chromosome region 17q12-q22. Additionally, part, if not all, of the nerve growth factor receptor locus...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.5.1403

    authors： Huebner K,Isobe M,Chao M,Bothwell M,Ross AH,Finan J,Hoxie JA,Sehgal A,Buck CR,Lanahan A

    更新日期：1986-03-01 00:00:00

  • Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores.

    abstract：:Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. "Self-healing" dyes, in which triplet states are intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness and longevity, while simultaneously reducing the generation of reactive oxygen ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2006517117

    authors： Pati AK,El Bakouri O,Jockusch S,Zhou Z,Altman RB,Fitzgerald GA,Asher WB,Terry DS,Borgia A,Holsey MD,Batchelder JE,Abeywickrama C,Huddle B,Rufa D,Javitch JA,Ottosson H,Blanchard SC

    更新日期：2020-09-29 00:00:00

  • Protein phosphorylation and sodium conductance in nerve membrane.

    abstract：:High molecular weight proteins extracted from the walking nerves of the shore crab (Carcinus maenas) exhibit a cycle of phosphorylation-dephosphorylation that is influenced by neurotropic compounds and inorganic ions. The net phosphorylation state of the proteins is increased in the presence of K+ ions and decreased w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.2.812

    authors： Schoffeniels E,Dandrifosse G

    更新日期：1980-02-01 00:00:00

  • TNF receptor (TNFR)-associated factor (TRAF) 3 serves as an inhibitor of TRAF2/5-mediated activation of the noncanonical NF-kappaB pathway by TRAF-binding TNFRs.

    abstract：:TNF family members and their receptors contribute to increased gene expression for inflammatory processes and intracellular cascades leading to programmed cell death, both via activation of NF-kappaB. TNF receptor (TNFR)-associated factors (TRAFs) are cytoplasmic adaptor proteins binding to various receptors of the TN...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0500187102

    authors： Hauer J,Püschner S,Ramakrishnan P,Simon U,Bongers M,Federle C,Engelmann H

    更新日期：2005-02-22 00:00:00

  • Observation of an apparent first-order glass transition in ultrafragile Pt-Cu-P bulk metallic glasses.

    abstract：:An experimental study of the configurational thermodynamics for a series of near-eutectic Pt80-x Cu x P20 bulk metallic glass-forming alloys is reported where 14 < x < 27. The undercooled liquid alloys exhibit very high fragility that increases as x decreases, resulting in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1916371117

    authors： Na JH,Corona SL,Hoff A,Johnson WL

    更新日期：2020-02-11 00:00:00

  • Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells.

    abstract：:Vasopressin's action in renal cells to regulate water transport depends on protein phosphorylation. Here we used mass spectrometry-based quantitative phosphoproteomics to identify signaling pathways involved in the short-term V2-receptor-mediated response in cultured collecting duct cells (mpkCCD) from mouse. Using St...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910646107

    authors： Rinschen MM,Yu MJ,Wang G,Boja ES,Hoffert JD,Pisitkun T,Knepper MA

    更新日期：2010-02-23 00:00:00

  • Epidermal growth factor stimulates tyrosine phosphorylation in the neonatal mouse: association of a M(r) 55,000 substrate with the receptor.

    abstract：:Administration of epidermal growth factor (EGF) to neonatal mice resulted in rapid tyrosine phosphorylation of a number of specific substrates in liver, kidney, lung, bladder, skin, and brain as detected by Western blot analysis of tissue homogenates with anti-phosphotyrosine antibodies. In the liver, three prominent ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.18.8477

    authors： Donaldson RW,Cohen S

    更新日期：1992-09-15 00:00:00

  • Supercoiling of the DNA template during transcription.

    abstract：:Transcription of a right-handed double-helical DNA requires a relative rotation of the RNA polymerase and its nascent RNA around the DNA. We describe conditions under which the resistance to the rotational motion of the transcription ensemble around the DNA can be large. In such cases, the advancing polymerase generat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.20.7024

    authors： Liu LF,Wang JC

    更新日期：1987-10-01 00:00:00

  • Effects of past, present, and future ocean carbon dioxide concentrations on the growth and survival of larval shellfish.

    abstract：:The combustion of fossil fuels has enriched levels of CO(2) in the world's oceans and decreased ocean pH. Although the continuation of these processes may alter the growth, survival, and diversity of marine organisms that synthesize CaCO(3) shells, the effects of ocean acidification since the dawn of the industrial re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0913804107

    authors： Talmage SC,Gobler CJ

    更新日期：2010-10-05 00:00:00

  • Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch.

    abstract：:Because RNAs fold as they are being synthesized, their transcription rate can affect their folding. Here, we report the results of single-molecule fluorescence studies that characterize the ligand-dependent cotranscriptional folding of the Escherichia coli thiM riboswitch that regulates translation. We found that the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1712983115

    authors： Uhm H,Kang W,Ha KS,Kang C,Hohng S

    更新日期：2018-01-09 00:00:00

  • CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein.

    abstract：:Members of the tumor necrosis factor receptor (TNFR) superfamily are important for cell growth and survival. In addition to providing costimulatory signals for cell proliferation, ligation of both TNFR1 and Fas can result in programmed cell death or apoptosis. The underlying mechanism requires an intact 80-aa stretch ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.12.6346

    authors： Prasad KV,Ao Z,Yoon Y,Wu MX,Rizk M,Jacquot S,Schlossman SF

    更新日期：1997-06-10 00:00:00

  • Agricultural intensification escalates future conservation costs.

    abstract：:The supposition that agricultural intensification results in land sparing for conservation has become central to policy formulations across the tropics. However, underlying assumptions remain uncertain and have been little explored in the context of conservation incentive schemes such as policies for Reducing Emission...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1220070110

    authors： Phelps J,Carrasco LR,Webb EL,Koh LP,Pascual U

    更新日期：2013-05-07 00:00:00

  • Chemical composition of Earth, Venus, and Mercury.

    abstract：:Model compositions of Earth, Venus, and Mercury are calculated from the premise that planets and chondrites underwent four identical fractionation processes in the solar nebula. Because elements of similar properties stay together in these processes, five constraints suffice to define the composition of a planet: mass...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.12.6973

    authors： Morgan JW,Anders E

    更新日期：1980-12-01 00:00:00

  • Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1.

    abstract：:Glucagon-like peptide-1 (GLP-1), released from gut endocrine L cells in response to glucose, regulates appetite, insulin secretion, and gut motility. How glucose given orally, but not systemically, induces GLP-1 secretion is unknown. We show that human duodenal L cells express sweet taste receptors, the taste G protei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0706890104

    authors： Jang HJ,Kokrashvili Z,Theodorakis MJ,Carlson OD,Kim BJ,Zhou J,Kim HH,Xu X,Chan SL,Juhaszova M,Bernier M,Mosinger B,Margolskee RF,Egan JM

    更新日期：2007-09-18 00:00:00

  • Reduced cortical inhibition in a mouse model of familial childhood absence epilepsy.

    abstract：:Mutations in the GABA(A) receptor gamma2 subunit are associated with childhood absence epilepsy and febrile seizures. To understand better the molecular basis of absence epilepsy in man, we developed a mouse model harboring a gamma2 subunit point mutation (R43Q) found in a large Australian family. Mice heterozygous fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0708440104

    authors： Tan HO,Reid CA,Single FN,Davies PJ,Chiu C,Murphy S,Clarke AL,Dibbens L,Krestel H,Mulley JC,Jones MV,Seeburg PH,Sakmann B,Berkovic SF,Sprengel R,Petrou S

    更新日期：2007-10-30 00:00:00

  • Cooperative foraging, productivity, and the central limit theorem.

    abstract：:The central limit theorem is applied to group foraging to show an automatic and universal benefit to group living. This may explain the paradoxical inverse correlation between group size and per capita brood production in primitively eusocial insects and why only one of the five major lineages of social insects contai...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.1.36

    authors： Wenzel JW,Pickering J

    更新日期：1991-01-01 00:00:00

  • D-serine, an endogenous synaptic modulator: localization to astrocytes and glutamate-stimulated release.

    abstract：:Using an antibody highly specific for D-serine conjugated to glutaraldehyde, we have localized endogenous D-serine in rat brain. Highest levels of D-serine immunoreactivity occur in the gray matter of the cerebral cortex, hippocampus, anterior olfactory nucleus, olfactory tubercle, and amygdala. Localizations of D-ser...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.9.3948

    authors： Schell MJ,Molliver ME,Snyder SH

    更新日期：1995-04-25 00:00:00

  • Reduction of mammalian ferritin.

    abstract：:Mammalian ferritin from horse spleen undergoes an electrochemical or chemical reduction reaction in which each iron atom present is reduced by one electron (2300 electrons per ferritin molecule containing 2300 Fe3+ ions). Midpoint potentials of -190 mV, -310 mV, and -416 mV were determined at pH 7.0, 8.0, and 9.0. Thi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.11.3640

    authors： Watt GD,Frankel RB,Papaefthymiou GC

    更新日期：1985-06-01 00:00:00

  • Relative abundance of Alzheimer A beta amyloid peptide variants in Alzheimer disease and normal aging.

    abstract：:The Alzheimer A beta amyloid peptide (A beta) is the principal proteinaceous component of amyloid associated with Alzheimer disease (AD). We have determined the relative abundance of A beta structural variants present in amyloid from brains of 10 individuals with sporadic AD, 2 individuals with familial AD carrying sp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.18.8378

    authors： Näslund J,Schierhorn A,Hellman U,Lannfelt L,Roses AD,Tjernberg LO,Silberring J,Gandy SE,Winblad B,Greengard P

    更新日期：1994-08-30 00:00:00