Abstract:
PURPOSE:To quantitate in vivo retinal ganglion cell (RGC) survival after transient periods of pressure-induced ischemia of the rat retina and after different survival intervals. METHODS:In adult rats, RGCs were labeled with fluorogold applied to their main targets in the brain. Seven days later, in several groups of rats, the left retinas were subjected to transient periods of ischemia of 30, 45, 60, 75, 90, 105, or 120 minutes, respectively, by increasing the intraocular pressure (IOP) of the left eye above systolic values. Five, 7, 14, and 30 days later, the rats were killed, and their retinas were prepared as wholemounts for examination under fluorescence microscopy to estimate RGC survival. RESULTS:The authors found that periods of ischemia of 30 and 45 minutes do not induce RGC death; longer periods of transient ischemia induce the death of a proportion of RGCs, and the proportion increases with the duration of the ischemia; RGC death, which can be observed as early as 5 days after ischemia, continues during the 30-day study period; and periods of ischemia that last 90 minutes or more cause the death of approximately 95% of the RGC population 30 days later. CONCLUSIONS:Increases of the IOP above systolic levels for periods of 60 minutes or more result in RGC loss in the rat retina. Both the duration of the initial transient period of ischemia and the duration of the survival period influence the proportion of RGC death.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Sellés-Navarro I,Villegas-Pérez MP,Salvador-Silva M,Ruiz-Gómez JM,Vidal-Sanz Msubject
Has Abstractpub_date
1996-09-01 00:00:00pages
2002-14issue
10eissn
0146-0404issn
1552-5783journal_volume
37pub_type
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journal_title:Investigative ophthalmology & visual science
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pub_type: 杂志文章,多中心研究
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journal_title:Investigative ophthalmology & visual science
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更新日期:1978-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:2002-11-01 00:00:00