Abstract：
:Short tandem repeat (STR) analysis is increasingly being used in forensic case analysis because of the large number of STR loci in the human genome and their highly polymorphic nature. An automated DNA sequencer using high sensitivity infrared (IR) fluorescence technology was used to detect STR allele patterns from simulated forensic samples. The amplification strategy used a 19 base pair extension on the 5' end of one of the PCR primers. This sequence is identical to the sequence of a universal M13 Forward sequencing primer which is included in the amplification reaction. Allelic bands were detected by incorporation of the M13 primer-fluorescent dye conjugate into PCR products thus eliminating the need for direct conjugation of fluorescent dye to individual STR primers. By using an IR-based automated DNA sequencer and Tth DNA polymerase, polymorphic STR alleles were detected on-line rapidly and efficiently from bloodstains using only a high temperature incubation to extract DNA from blood cells. Five STR loci were also amplified using Chelex extracted DNA from simulated forensic samples. Multiplexing of three primer pairs in a single PCR mixture for amplification was accomplished using Taq polymerase. This system combines IR fluorescence chemistry and laser technology thus eliminating the need for radioactivity and the gel handling required with silver staining and fluor detection systems. Real-time detection permits immediate visualization of the data and STR alleles are displayed as familiar autoradiogramlike images that can be analyzed by computer. By loading a 64 lane gel twice and multiplexing with three primer pairs, forensic scientists can type at least three loci from 120 samples in one day.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Roy R,Steffens DL,Gartside B,Jang GY,Brumbaugh JAsubject
Has Abstractpub_date
1996-05-01 00:00:00pages
418-24issue
3eissn
0022-1198issn
1556-4029journal_volume
41pub_type
杂志文章abstract：:The duty to protect, or Tarasoff duty, has been conceptualized as arising solely in the context of a clinical setting. A recent California Supreme Court ruling in People v. Clark adds legal, clinical, and ethical dilemmas to the oftentimes contentious Tarasoff issue. Though the Tarasoff issue is but a minor legal poin...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:One hundred thirty cases of sudden infant death occurring in Wayne County, Michigan, (population 2.7 million) were analyzed for possible drugs. The toxicological protocol has been outlined. Six cases were found to be positive, and in five of these the drugs found had been prescribed for a variety of illnesses. In one ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1977-10-01 00:00:00
abstract：:This study concentrates on samples of bare pork skin, with and without bristles, and dried bovine ribs shot with a semi-automatic pistol to find the best methodology and sampling surface in the search for inorganic gunshot residues (IGSR). Four quadrants of known surface areas were sampled at different distances from ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13870
更新日期：2019-03-01 00:00:00
abstract：:Internal standards are commonly used for the quantitative determination of drugs of abuse and their metabolites (drug/metabolite) in biological fluids and tissues by the selective ion monitoring (SIM) gas chromatography/mass spectrometry (GC/MS) procedure. Analogs of drugs/metabolites that are labeled with three or mo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Due to increasing caseloads and inadequate staffing, the burden on Coroner/Medical Examiner Offices to comply with recommended autopsy limits for forensic pathologists (FPs) has been difficult. Since 2006, pathologists at the University of Alabama at Birmingham have performed select autopsies for the Alabama Departmen...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13269
更新日期：2017-01-01 00:00:00
abstract：:This study was conducted to collect data on specific volatile fatty acids (produced from soft tissue decomposition) and various anions and cations (liberated from soft tissue and bone), deposited in soil solution underneath decomposing human cadavers as an aid in determining the "time since death." Seven nude subjects...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Five South Portuguese Caucasian subpopulations were analyzed for the HLA-DQA1, LDLR, GYPA, HBGG, D7S8 and Gc loci. Genotype distributions for these loci did not deviate from Hardy-Weinberg expectations. The allele and genotype frequencies found have been compared with previously published data from North and Central P...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:A 38-year-old nondiabetic female developed fatal hypoglycemia when chlorpropamide (Diabinese) was accidentally substituted for acetaminophen (Tylenol) with codeine no. 3 in a pharmacy dispensing error. When found, the patient's serum glucose was less than 20 mg/dL. The serum chlorpropamide level on hospital admission ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The current study investigates the removal of soft tissues from mice and rats by the use of three different proteases and one lipase from Novozymes A/S. The results demonstrate the enzyme maceration to be remarkably fast (1-3 h) compared to the traditional warm-water procedure, which requires up to several days. In ad...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01668.x
更新日期：2011-03-01 00:00:00
abstract：:As issues of professional standards and error rates continue to be addressed in the courts, forensic anthropologists should be proactive by developing and adhering to professional standards of best practice. There has been recent increased awareness and interest in critically assessing some of the techniques used by f...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01176.x
更新日期：2009-11-01 00:00:00
abstract：:In cases where there is limited antemortem information, the examination of unidentified human remains as part of the investigation of long-term missing person's cases is a complex endeavor and consequently requires a multidisciplinary approach. Bomb pulse dating, which involves the analysis and interpretation of 14C c...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：10.1111/1556-4029.14227
更新日期：2020-05-01 00:00:00
abstract：:This research examined differences in decomposition rate and manner of domestic pig subjects (Sus scrofa) in never frozen (control) and previously frozen (experimental) research conditions. Eight control and experimental subjects were placed in an identical outdoor research environment. Daily quantitative and qualitat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12717
更新日期：2015-05-01 00:00:00
abstract：:Detection of ketamine exposure in skeletal tissues by automated enzyme-linked immunosorbent assay (ELISA) and gas chromatography with electron capture detection (GC-ECD) is described. Rats (n = 18) received 0, 15, 30, or 75 mg/kg ketamine hydrochloride acutely (i.p.), and were euthanized within 15 min or 1 h. Ketamine...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00866.x
更新日期：2008-11-01 00:00:00
abstract：:Thromboemboli were found diffusely throughout the pulmonary vasculature system of a 76-year-old white female who died unexpectedly 3 days following injury and repair of a left hip intracapsular fracture. A diffuse chronic nonspecific myocarditis with marked fat atrophy, an acute myocardial infarct of the posterior lef...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:DNA is often difficult to extract from old bones and teeth due to low levels of DNA and high levels of degradation. This study established a simple yet efficient method for extracting DNA from 20 aged bones and teeth (approximately 60 years old). Based on the concentration and STR typing results, the new method of DNA...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13603
更新日期：2018-05-01 00:00:00
abstract：:These studies were designed to demonstrate that the usual method of laryngeal dissection carries a significant risk of overlooking important laryngeal injuries. Formalin-fixed hyoid-larynx complexes were prepared in a prospective forensic study involving 191 cases of homicidal strangulation, 1984 to 1997. The basic st...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:As drug overdose deaths across the United States continue to rise, there is increasing interest in field testing of illicit substances. This work discusses a paper-based analytical device (idPAD) that can run a library of 12 colorimetric tests at the same time, each detecting different chemical functional groups and m...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14318
更新日期：2020-07-01 00:00:00
abstract：:The paper is concerned with the strength of DNA evidence when a suspect is identified via a search through a database of the DNA profiles of known individuals. Consideration of the appropriate likelihood ratio shows that in this setting the DNA evidence is (slightly) stronger than when a suspect is identified by other...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:At the turn of the millennium, the authors summarize the evolution of a clinician's duty to protect third persons from a patient's violent acts over the past half century, with special emphasis on jurisprudential developments in the last decade. Four evolutionary periods are identified: Pre-Tarasoff, Inception, Divers...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：2001-09-01 00:00:00
abstract：:The acetabulum/pubis index was tested on American Eskimo and Indian samples of innominate bones to which sex had been assigned by visual inspection. The index alone sexed 91 and 89% of the Eskimo and Indian samples, respectively. Subsequent sorting of misclassified specimens with femur head diameter resulted in 96 and...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Prior to 1984, William R. Maples, Ph.D. worked primarily with Medical Examiners in the State of Florida in investigation of and testimony in criminal cases. In 1984 the Republic of Peru requested him to identify skeletal remains thought to be those of Francisco Pizarro, conqueror of Peru and the Incas in the early 16t...
journal_title：Journal of forensic sciences
pub_type： 传,历史文章,杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:The development of less-lethal technologies has provided law enforcement personnel with an alternative to lethal force. Although the less lethal projectile was produced to engender non-penetrating wounds, case studies show that there have been a number of reported penetrating injuries ranging from minor to significant...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:This paper reviews the complex pharmacology of the new class of antidepressant medications exhibiting selective inhibition of serotonin reuptake. The four selective serotonin reuptake inhibitors (SSRIs) considered--fluoxetine, fluvoxamine, sertraline and paroxetine--can result in toxicity and death through contributin...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：2000-05-01 00:00:00
abstract：:DNA extracted from fingernail clippings of victims in forensic cases is a possible source of DNA from the perpetrator in cases where victims struggled or defended themselves. The source of this DNA on a victim's fingernails could possibly originate from contact with the suspect's blood, saliva, semen or scratched skin...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:A few sexual fatalities show ambiguous or conflicting evidence of manner of death or, in cases involving partners, of the partner's intent. In such equivocal cases, postmortem behavioral analysis and reconstruction aid in understanding what happened and provide an explainable basis for expert judgment and opinion, eve...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:To establish a useful laboratory protocol to investigate possible cases of fatal anaphylaxis, we measured mast-cell-derived tryptase levels and allergen-specific immunoglobulin E (IgE) antibody levels in sera obtained prior to or within 24 h after death from 19 anaphylaxis victims. Elevated serum tryptase levels (rang...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:The Phenice method for the estimation of sex of an individual from the pelvis was tested on 198 individuals of known sex from the Terry collection by an individual with training in the technique, but no additional training in forensic anthropology. Sex was correctly estimated in 88.4% of all individuals and more accur...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The interpretation of postmortem cocaine concentrations is made in an attempt to estimate drug concentrations present at the time of death and thus infer not only drug presence but drug toxicity. Previous data suggest that changes in postmortem blood cocaine concentrations over time are not predictable and interpretat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Over the past decade, there has been a growing openness about the importance of human factors in forensic work. However, most of it focused on cognitive bias, and neglected issues of workplace wellness and stress. Forensic scientists work in a dynamic environment that includes common workplace pressures such as worklo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13533
更新日期：2018-01-01 00:00:00
abstract：:For the past 10 years, medical imaging techniques have been increasingly applied to forensic investigations. To obtain histological and toxicological information, tissue and liquid samples are required. In this article, we describe the development of a low-cost, secure, and reliable approach for a telematic add-on for...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01990.x
更新日期：2012-03-01 00:00:00
