Thapsigargin, a weak skin tumor promoter, alters the growth and differentiation of mouse keratinocytes in culture.


:Thapsigargin (Tg), applied twice weekly to the backs of CD-1 mice initiated with 7,12-dimethylbenz[a]anthracene, promoted papillomas on the skin of approximately 50% of the mice. Tg alone induced papillomas in 10% of the mice. Although Tg and 12-O-tetradecanoylphorbol-13-acetate (TPA) are synergistic in a keratinocyte co-culture assay for promotion, skin tumor promotion by TPA was inhibited by co-treatment with Tg. Treatment of cultured keratinocytes with non-toxic doses of Tg increased the level of intracellular free Ca(2+)-induced stratification. Tg blocked expression of the spinous layer differentiation marker keratin 1 (K1), while inducing cornification, a marker of differentiation in the granular layer/stratum corneum. In medium with 1.4 mM Ca2+, Tg prolonged keratinocyte proliferation and selected foci of monolayer cells. A Tg-independent cell line, TK-1, was developed from a single dish in which foci continued to expand after Tg removal. Grafting TK-1 cells on to the backs of nude mice as part of a reconstituted skin resulted in the development of dysplastic papillomas with a high rate of progression to squamous cell carcinomas.






Lowry DT,Li L,Hennings H




Has Abstract


1996-04-01 00:00:00












  • Endocrine dysfunction in p27Kip1 deficient mice and susceptibility to Wnt-1 driven breast cancer.

    abstract::The cyclin-dependent kinase (Cdk) inhibitor p27(Kip1) (p27) is a marker of prognosis in many cancers, including breast cancer. Low p27 expression correlates with poor prognosis, especially in hormone receptor positive breast tumors. This association suggests a role for p27 in hormone-dependent cancer. We used the Wnt-...


    pub_type: 杂志文章


    authors: Glover CE,Gurley KE,Kim KH,Storer B,Fero ML,Kemp CJ

    更新日期:2009-06-01 00:00:00

  • Xeroderma pigmentosum group A gene action as a protection factor against 4-nitroquinoline 1-oxide-induced tongue carcinogenesis.

    abstract::To test the hypothesis that nucleotide excision repair (NER) plays a protective role in chemical carcinogenesis in internal organs, xeroderma pigmentosum group A gene-deficient (XPA(-/-)) mice, heterozygous (XPA(+/-)) and wild-type (XPA(+/+)) mice were orally administered 0.001% 4-nitroquinoline 1-oxide (4NQO) in thei...


    pub_type: 杂志文章


    authors: Ide F,Oda H,Nakatsuru Y,Kusama K,Sakashita H,Tanaka K,Ishikawa T

    更新日期:2001-04-01 00:00:00

  • Over-expression of EphB3 enhances cell-cell contacts and suppresses tumor growth in HT-29 human colon cancer cells.

    abstract::Receptor tyrosine kinase EphB3 is expressed in cells in the bottom of intestinal crypts near stem cell niches. Loss of Ephb3 has recently been reported to produce invasive colorectal carcinoma in Apc(Min/+) mice and EphB-mediated compartmentalization was demonstrated to be a mechanism suppressing colorectal cancer pro...


    pub_type: 杂志文章


    authors: Chiu ST,Chang KJ,Ting CH,Shen HC,Li H,Hsieh FJ

    更新日期:2009-09-01 00:00:00

  • Benzo[a]pyrene metabolites: formation in rat liver cell-culture lines, binding to macromolecules, and mutagenesis in V79 hamster cells.

    abstract::Benzo(a]pyrene was metabolized in liver cell lines derived from BC-IV and BD-IV rats which included several chemically-transformed lines (IAR-6-1; IAR-19; IAR-28), one spontaneous transformant (IAR-27) as well as one non-malignant line (IAR-20). Cultures were treated with tritiated benzo[a]pyrene over a 5-day period. ...


    pub_type: 杂志文章


    authors: Selkirk JK,MacLeod MC,Kuroki T,Drevon C,Piccoli C,Montesano R

    更新日期:1982-01-01 00:00:00

  • Carcinogenicity studies of masheri, a pyrolysed product of tobacco.

    abstract::The carcinogenicity of two commonly used brown and black varieties of masheri, a pyrolysed tobacco product, was studied by feeding the masheri through the diet at a 10% level to three different animal species of both sexes. In Sprague-Dawley rats, only brown masheri was used, while in Swiss mice and Syrian golden hams...


    pub_type: 杂志文章


    authors: Kulkarni JR,Lalitha VS,Bhide SV

    更新日期:1988-11-01 00:00:00

  • A trans-fatty acid-rich diet promotes liver tumorigenesis in HCV core gene transgenic mice.

    abstract::Excess consumption of trans-fatty acid (TFA), an unsaturated fatty acid containing trans double bonds, is a major risk factor for cardiovascular disease and metabolic syndrome. However, little is known about the link between TFA and hepatocellular carcinoma (HCC) despite it being a frequent form of cancer in humans. I...


    pub_type: 杂志文章


    authors: Hu X,Wang X,Jia F,Tanaka N,Kimura T,Nakajima T,Sato Y,Moriya K,Koike K,Gonzalez FJ,Nakayama J,Aoyama T

    更新日期:2020-04-22 00:00:00

  • Inhibition of 7,12-dimethylbenz[a]anthracene-induced genotoxicity in Chinese hamster ovary cells by retinol and retinoic acid.

    abstract::The effects of retinol and retinoic acid on cytotoxicity and mutation expression at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells with or without exogenous metabolic activation were studied in the presence and absence of known chemical mutagens. Neither retinol ...


    pub_type: 杂志文章


    authors: Budroe JD,Schol HM,Shaddock JG,Casciano DA

    更新日期:1988-07-01 00:00:00

  • A new sensitive 32P-postlabeling assay based on the specific enzymatic conversion of bulky DNA lesions to radiolabeled dinucleotides and nucleoside 5'-monophosphates.

    abstract::A new sensitive 32P-postlabeling assay for DNA adducts has been developed in which DNA is hydrolyzed initially by nuclease P1 and prostatic acid phosphatase instead of micrococcal nuclease and spleen phosphodiesterase as employed in previous postlabeling procedures. When DNA containing bulky adducts, X1, X2, .....Xn, ...


    pub_type: 杂志文章


    authors: Randerath K,Randerath E,Danna TF,van Golen L,Putman KL

    更新日期:1989-07-01 00:00:00

  • The Escherichia coli MutS DNA mismatch binding protein specifically binds O(6)-methylguanine DNA lesions.

    abstract::DNA mismatch repair defects in certain cell types confer resistance to the cytotoxic effects of alkylating agents, suggesting that a normally functioning DNA mismatch repair pathway can actually mediate alkylation-induced cell death. In eukaryotic cells this phenomenon is only observed in cells lacking adequate DNA me...


    pub_type: 杂志文章


    authors: Rasmussen LJ,Samson L

    更新日期:1996-09-01 00:00:00

  • The p53 target gene desmocollin 3 acts as a novel tumor suppressor through inhibiting EGFR/ERK pathway in human lung cancer.

    abstract::Desmosomes are intercellular junctions that confer strong cell-cell adhesion. Altered expression of desmocollin 3 (DSC3), a member of the desmosomal cadherin family, was found in various cancers; however, its functional involvement in carcinogenesis has not yet been elucidated. Expression/localization of DSC3 was anal...


    pub_type: 杂志文章


    authors: Cui T,Chen Y,Yang L,Knösel T,Huber O,Pacyna-Gengelbach M,Petersen I

    更新日期:2012-12-01 00:00:00

  • Epidermal papillomas and carcinomas induced in uninitiated mouse skin by tumor promoters alone contain a point mutation in the 61st codon of the Ha-ras oncogene.

    abstract::Previous results in a number of laboratories have demonstrated that epidermal papillomas and carcinomas induced by the two-stage protocol of initiation and promotion contain a point mutation in the 61st codon of the c-Ha-ras oncogene when the initiating agent used is 7,12-dimethylbenz[a]anthracene (DMBA). In the prese...


    pub_type: 杂志文章


    authors: Pelling JC,Neades R,Strawhecker J

    更新日期:1988-04-01 00:00:00

  • Mutational specificities of environmental carcinogens in the lacI gene of Escherichia coli. VI: Analysis of methylene chloride-induced mutational distribution in Uvr+ and UvrB- strains.

    abstract::To better understand the mechanisms of mutagenesis by the carcinogen, methylene chloride (DCM), we have determined the nature and distribution of forward mutations induced by DCM in the N-terminal region of the lacI gene of Escherichia coli. A total of 116 lacI-d mutations (50 from Uvr+, 66 from UvrB- strain) were cha...


    pub_type: 杂志文章


    authors: Zielenska M,Ahmed A,Pienkowska M,Anderson M,Glickman BW

    更新日期:1993-05-01 00:00:00

  • Inhibitory effect of curcumin on 12-O-tetradecanoylphorbol-13-acetate-induced increase in ornithine decarboxylase mRNA in mouse epidermis.

    abstract::Application of 5 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) to the skin of female CD-1 mice led to a rapid increase in the concentration of epidermal ornithine decarboxylase (ODC) mRNA from an undetectable level in control mice to a high maximum level at 4-5 h after TPA administration. The concentration of epider...


    pub_type: 杂志文章


    authors: Lu YP,Chang RL,Huang MT,Conney AH

    更新日期:1993-02-01 00:00:00

  • The ADAM17 protease promotes tobacco smoke carcinogen-induced lung tumorigenesis.

    abstract::Lung cancer is the leading cause of cancer-related mortality, with most cases attributed to tobacco smoking, in which nicotine-derived nitrosamine ketone (NNK) is the most potent lung carcinogen. The ADAM17 protease is responsible for the ectodomain shedding of many pro-tumorigenic cytokines, growth factors and recept...


    pub_type: 杂志文章


    authors: Saad MI,McLeod L,Yu L,Ebi H,Ruwanpura S,Sagi I,Rose-John S,Jenkins BJ

    更新日期:2020-06-17 00:00:00

  • Instability of (+/-)-7 beta,8 alpha-dihydroxy-9 beta,10 beta-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (syn-BaPDE)-DNA adducts formed in benzo[a]pyrene-treated Wistar rat embryo cell cultures.

    abstract::One of the peaks present in HPLC profiles of [3H]benzo[a]-pyrene (BaP)-deoxyribonucleosides prepared by enzymatic degradation of [3H]BaP-DNA isolated from Wistar rat embryo cell cultures exposed to [G-3H]BaP was found to be r-7,c-9,c-10,t-8-tetrahydroxy-7,8,9,10-tetrahydroBaP, a BaP-DNA adduct decomposition product (P...


    pub_type: 杂志文章


    authors: Pruess-Schwartz D,Mauthe RJ,Baird WM

    更新日期:1988-10-01 00:00:00

  • Novel therapies hijack the blood-brain barrier to eradicate glioblastoma cancer stem cells.

    abstract::Glioblastoma (GBM) is amongst the most aggressive brain tumors with a dismal prognosis. Despite significant advances in the current multimodality therapy including surgery, postoperative radiotherapy (RT) and temozolomide (TMZ)-based concomitant and adjuvant chemotherapy (CT), tumor recurrence is nearly universal with...


    pub_type: 杂志文章,评审


    authors: Vengoji R,Ponnusamy MP,Rachagani S,Mahapatra S,Batra SK,Shonka N,Macha MA

    更新日期:2019-03-12 00:00:00

  • CELF2 suppresses non-small cell lung carcinoma growth by inhibiting the PREX2-PTEN interaction.

    abstract::The phosphoinositide 3-kinase (PI3-K)/Akt signaling pathway is important in the regulation of cell proliferation through its production of phosphatidylinositol 3,4,5-triphosphate (PIP3). Activation of this pathway is frequently observed in human cancers, including non-small cell lung carcinoma. The PI3-K/Akt pathway i...


    pub_type: 杂志文章


    authors: Yeung YT,Fan S,Lu B,Yin S,Yang S,Nie W,Wang M,Zhou L,Li T,Li X,Bode AM,Dong Z

    更新日期:2020-05-14 00:00:00

  • Dibenz[a,h]anthracene-induced subcutaneous tumors in mice. Strain sensitivity and the role of carcinogen metabolism.

    abstract::Four inbred strains of mice (C3H/HeJ, C57BL/6J, AKR/J, and DBA/2J) were injected s.c. with 150 micrograms of dibenz[a,h]anthracene and followed for 9 months. Strains C3H/HeJ and C57BL/6J were most susceptible to dibenz[a,h]anthracene-induced carcinogenesis with 80% and 53% incidence of tumors, respectively. Strains AK...


    pub_type: 杂志文章


    authors: Lubet RA,Connolly GM,Nebert DW,Kouri RE

    更新日期:1983-01-01 00:00:00

  • The induction of Ouar-mutations in nontransformable CVP3SC6 mouse fibroblasts.

    abstract::A line of C3H mouse fibroblasts designated CVP3SC6 (CVP) was found to be nonresponsive to the induction of morphological transformation by the polycyclic aromatic hydrocarbon benzo[a]pyrene (BaP) and its BaP-7,8-diol and BaP-diol-epoxide derivates. This cell line was, however, susceptible to BaP-induced mutation to ou...


    pub_type: 杂志文章


    authors: Gehly EB,Heidelberger C

    更新日期:1982-01-01 00:00:00

  • Cell specificity of rat cytokeratin p39 during azo dye-induced hepatocarcinogenesis.

    abstract::Monoclonal or affinity-purified antibodies specific to Novikoff hepatoma cytokeratin p39 were employed to study the origin and fate of p39-containing cell types during hepatocarcinogenesis induced with N,N-dimethyl-p(m-tolylazo)aniline. Frozen sections were obtained from the livers of animals autopsied temporally duri...


    pub_type: 杂志文章


    authors: Schmidt WN,Page DL,McKusick K,Hnilica LS

    更新日期:1985-08-01 00:00:00

  • Metabolic cooperation in SK-HEP-1 human hepatoma cells following treatment with benzodiazepine tranquilizers.

    abstract::The ability of three benzodiazepine tranquilizers, diazepam, oxazepam and chlordiazepoxide chlorhydrate to block metabolic cooperation between human hepatoma cells was analysed using autoradiographical monitoring. Independent tests were performed with compound concentrations varying from 1 to 50 micrograms/ml. The res...


    pub_type: 杂志文章


    authors: Rolin-Limbosch S,Moens W,Szpirer C

    更新日期:1987-07-01 00:00:00

  • HTLV-1 bZIP factor impedes the menin tumor suppressor and upregulates JunD-mediated transcription of the hTERT gene.

    abstract::Telomerase activity in cancer cells is dependent on the transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene, encoding the catalytic subunit of human telomerase. We have shown previously that HTLV-1 basic leucine zipper (HBZ), a viral regulatory protein encoded by the human retrovirus,...


    pub_type: 杂志文章


    authors: Borowiak M,Kuhlmann AS,Girard S,Gazzolo L,Mesnard JM,Jalinot P,Dodon MD

    更新日期:2013-11-01 00:00:00

  • Can supplementation of phytoestrogens/insoluble fibers help the management of duodenal polyps in familial adenomatous polyposis?

    abstract::Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disorder, and prophylactic colectomy has been shown to decrease the incidence of colorectal cancer (CRC). Duodenal cancer and desmoids are now the leading causes of death in FAP. We evaluate whether 3 months of oral supplementation with a patented...


    pub_type: 临床试验,杂志文章


    authors: Calabrese C,Rizzello F,Gionchetti P,Calafiore A,Pagano N,De Fazio L,Valerii MC,Cavazza E,Strillacci A,Comelli MC,Poggioli G,Campieri M,Spisni E

    更新日期:2016-06-01 00:00:00

  • Population-specific genetic associations with oesophageal squamous cell carcinoma in South Africa.

    abstract::Genetic variants in multiple cellular pathways have been associated with an altered risk of oesophageal cancer. In this study, eight genes previously associated with an altered risk of oesophageal squamous cell carcinoma (OSCC) in European or Asian populations were investigated in two South African populations. We gen...


    pub_type: 杂志文章


    authors: Bye H,Prescott NJ,Matejcic M,Rose E,Lewis CM,Parker MI,Mathew CG

    更新日期:2011-12-01 00:00:00

  • BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer.

    abstract::BTG3/ANA/APRO4 has been reported to be a tumor suppressor gene in some malignancies. It constitutes important negative regulatory mechanism for Src-mediated signaling, a negative regulator of the cell cycle and inhibits transcription factor E2F1. We report that BTG3 is downregulated in renal cancer and that the mechan...


    pub_type: 杂志文章


    authors: Majid S,Dar AA,Ahmad AE,Hirata H,Kawakami K,Shahryari V,Saini S,Tanaka Y,Dahiya AV,Khatri G,Dahiya R

    更新日期:2009-04-01 00:00:00

  • Partial hepatectomy is a promoter of hepatocarcinogenesis in C57BL/6J male mice but not in C3H/HeJ male mice.

    abstract::We have shown previously that the difference between C57BL/6J and C3H/HeJ male mice in their susceptibilities to chemically-induced liver tumors results predominantly from an allelic difference at the Hepatocarcinogen sensitivity (Hcs) locus. This locus modulates the rate of growth of preneoplastic liver lesions and m...


    pub_type: 杂志文章


    authors: Hanigan MH,Winkler ML,Drinkwater NR

    更新日期:1990-04-01 00:00:00

  • Expression of the poly(ADP-ribose) polymerase gene following natural and induced DNA strand breakage and effect of hyperexpression on DNA repair.

    abstract::The catalytic activity of the nuclear enzyme poly(ADP-ribose) polymerase (NAD+ ADP-ribosyl transferase, EC 2,4,2,30) is totally dependent upon the presence of DNA strand breaks. Having isolated a full-length cDNA for the polymerase, we have now evaluated the effect of endogenously and exogenously induced DNA strand br...


    pub_type: 杂志文章


    authors: Bhatia K,Pommier Y,Giri C,Fornace AJ,Imaizumi M,Breitman TR,Cherney BW,Smulson ME

    更新日期:1990-01-01 00:00:00

  • Heme oxygenase expression in Swiss 3T3 cells following exposure to aqueous cigarette smoke fractions.

    abstract::A dose-dependent and transiently elevated expression of a cytoplasmic 32 kDa protein was observed in Swiss albino 3T3 fibroblasts exposed to mainstream cigarette smoke (CS) trapped in phosphate-buffered saline solutions (smoke-bubbled PBS). The protein was identified as heme oxygenase (HO) (heme, hydrogen donor:oxygen...


    pub_type: 杂志文章


    authors: Müller T,Gebel S

    更新日期:1994-01-01 00:00:00

  • Irreversible depression in the ratio of tetraploid:diploid liver nuclei in rats treated with 3'-methyl-4-dimethylaminoazobenzene (3'M).

    abstract::A reduction in the ratio of tetraploid to diploid liver nuclei has been investigated as an early indicator of hepatocarcinogenesis in the rat using the liver carcinogen 3'-methyl-4-dimethylaminoazobenzene (3'M). In a dose ranging study 3'M was administered by gavage to rats at 5, 12.5 and 25 mg/kg for up to 10 weeks a...


    pub_type: 杂志文章


    authors: Styles J,Elliott BM,Lefevre PA,Robinson M,Pritchard N,Hart D,Ashby J

    更新日期:1985-01-01 00:00:00

  • Differences in mediated mutagenesis and polycyclic aromatic hydrocarbon metabolism in mammary cells from pregnant and virgin rats.

    abstract::The physiological state of pregnancy confers significant resistance to polycyclic aromatic hydrocarbon (PAH)-induced mammary tumorigenesis. We have tested the abilities of primary mammary cells from pregnant and virgin rats cultured on collagen-coated plates to metabolize PAHs and convert these carcinogens to mutageni...


    pub_type: 杂志文章


    authors: Moore CJ,Gould MN

    更新日期:1984-01-01 00:00:00