Thapsigargin, a weak skin tumor promoter, alters the growth and differentiation of mouse keratinocytes in culture.


:Thapsigargin (Tg), applied twice weekly to the backs of CD-1 mice initiated with 7,12-dimethylbenz[a]anthracene, promoted papillomas on the skin of approximately 50% of the mice. Tg alone induced papillomas in 10% of the mice. Although Tg and 12-O-tetradecanoylphorbol-13-acetate (TPA) are synergistic in a keratinocyte co-culture assay for promotion, skin tumor promotion by TPA was inhibited by co-treatment with Tg. Treatment of cultured keratinocytes with non-toxic doses of Tg increased the level of intracellular free Ca(2+)-induced stratification. Tg blocked expression of the spinous layer differentiation marker keratin 1 (K1), while inducing cornification, a marker of differentiation in the granular layer/stratum corneum. In medium with 1.4 mM Ca2+, Tg prolonged keratinocyte proliferation and selected foci of monolayer cells. A Tg-independent cell line, TK-1, was developed from a single dish in which foci continued to expand after Tg removal. Grafting TK-1 cells on to the backs of nude mice as part of a reconstituted skin resulted in the development of dysplastic papillomas with a high rate of progression to squamous cell carcinomas.






Lowry DT,Li L,Hennings H




Has Abstract


1996-04-01 00:00:00












  • The activity of 3-methyladenine DNA glycosylate in animal tissues in relation to carcinogenesis.

    abstract::3-Methyladenine is one of the major products formed by reaction of a large number of environmental methylating agents with DNA in vivo and in vitro. In spite of the rapid spontaneous depurination of this base an enzyme, 3-methyladenine DNA glycosylase, has been shown to catalyse its excision. The relevance of this enz...


    pub_type: 杂志文章


    authors: Craddock VM,Henderson AR

    更新日期:1982-01-01 00:00:00

  • Adenylate cyclase activity in crude liver membranes during chemical hepatocarcinogenesis.

    abstract::Crude plasma membranes were prepared from the liver of control rats or of rats submitted to an initiation by diethyl-nitrosamine and selection with 2-acetylaminofluorene and carbon tetrachloride (group IS) or of rats submitted to an initiation-selection protocol followed by a promotion with phenobarbital (group IS PB)...


    pub_type: 杂志文章


    authors: Marino A,Ravelingien N,De Cannière D,Préat V,Dehaye JP,Pector JC

    更新日期:1991-04-01 00:00:00

  • Cyclin D1 gene polymorphism and susceptibility to lung cancer in a Chinese population.

    abstract::The objective was to study the relationship between cyclin D1 gene (CCND1) polymorphism and lung cancer in the Chinese population. Blood samples of 182 cases and 185 controls were collected from a hospital based case-control study. PCR-SSCP was used to examine the G/A polymorphism in exon 4 of CCND1. The results showe...


    pub_type: 杂志文章


    authors: Qiuling S,Yuxin Z,Suhua Z,Cheng X,Shuguang L,Fengsheng H

    更新日期:2003-09-01 00:00:00

  • Oxidation of 5'-site guanine at GG and GGG sequences induced by a metabolite of carcinogenic heterocyclic amine PhIP in the presence of Cu(II) and NADH.

    abstract::Adduct formation has been considered to be a major causal factor of DNA damage by carcinogenic heterocyclic amines. By means of experiments with an electrochemical detector coupled to a high-performance liquid chromatograph, we revealed that N-hydroxy metabolite of 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (Ph...


    pub_type: 杂志文章


    authors: Murata M,Kawanishi S

    更新日期:2002-05-01 00:00:00

  • Prostaglandin H synthase-mediated bioactivation of the amino acid pyrolysate product Trp P-2.

    abstract::We report evidence that the mutagen and carcinogen 3-amino-1-methyl-5H pyrido[4,3b]indole (Trp P-2) is a substrate for co-oxidation by prostaglandin H synthase (PHS) in ram seminal vesicle (RSV) microsomes. Trp P-2 serves as a reducing cofactor for the hydroperoxidase activity of PHS as shown by the concentration-depe...


    pub_type: 杂志文章


    authors: Petry TW,Krauss RS,Eling TE

    更新日期:1986-08-01 00:00:00

  • Expression of cytochrome P450 and microsomal epoxide hydrolase in cervical and oral epithelial cells immortalized by human papillomavirus type 16 E6/E7 genes.

    abstract::Epidemiological evidence suggests that the presence of human papillomaviruses (HPV), when combined with smoking behaviors, considerably enhances the risk of developing oral, cervical, vulvar, and/or anal carcinomas. It is well established that the cytochrome P450 (CYP), microsomal epoxide hydrolase (mEH), and other bi...


    pub_type: 杂志文章


    authors: Farin FM,Bigler LG,Oda D,McDougall JK,Omiecinski CJ

    更新日期:1995-06-01 00:00:00

  • In vivo accumulation of 8-hydroxy-2'-deoxyguanosine in DNA correlates with release of reactive oxygen species in Fanconi's anaemia families.

    abstract::The present study was aimed at verifying the occurrence, if any, of in vivo oxidative DNA damage in FA homozygotes, their parents and siblings. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) was measured, by HPLC/EC, in DNA from circulating blood leucocytes from FA homozygotes and their relatives and compared with a group of pa...


    pub_type: 杂志文章


    authors: Degan P,Bonassi S,De Caterina M,Korkina LG,Pinto L,Scopacasa F,Zatterale A,Calzone R,Pagano G

    更新日期:1995-04-01 00:00:00

  • Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine.

    abstract::DNA-demethylating agents activate tumor suppressor genes that are silenced by DNA methylation in cancer and are therefore emerging as a novel approach to cancer therapy. 5-azacytidine (VIDAZA), the first representative of this class of drugs was approved for treatment of myelodysplastic syndromes and is currently bein...


    pub_type: 杂志文章


    authors: Chik F,Machnes Z,Szyf M

    更新日期:2014-01-01 00:00:00

  • Molecular epidemiology, pathogenesis and prevention of gastric cancer.

    abstract::Cancer of the stomach is one of the most commonly diagnosed malignancies and remains an important cause of mortality world wide. This type of cancer is not uniformly distributed among populations but shows a marked variation in both incidence and mortality. Although gastric cancer is declining in many parts of the wor...


    pub_type: 杂志文章,评审


    authors: Stadtländer CT,Waterbor JW

    更新日期:1999-12-01 00:00:00

  • Nuclease sensitivity of repair-incorporated nucleotides in chromatin and nucleosome rearrangement in human cells damaged by methyl methanesulfonate and methylnitrosourea.

    abstract::We have examined both the initial nuclease sensitivity and subsequent nucleosome rearrangement of newly repaired regions of chromatin in human diploid fibroblasts treated with methyl methanesulfonate (MMS) and methylnitrosourea (MNU). We initially examined the effect of these two alkylating agents on DNA replicative s...


    pub_type: 杂志文章


    authors: Sidik K,Smerdon MJ

    更新日期:1984-02-01 00:00:00

  • Epigenetic therapy using the histone deacetylase inhibitor for increasing therapeutic gain in oral cancer: prevention of radiation-induced oral mucositis and inhibition of chemical-induced oral carcinogenesis.

    abstract::In addition to genetic changes, epigenetic aberrations also play important roles in radiation- and chemical-induced disorders and carcinogenesis. The present study investigated whether epigenetic therapy with a histone deacetylase (HDAC) inhibitor has dual benefits for radiation-induced oral mucositis and chemical-ind...


    pub_type: 杂志文章


    authors: Chung YL,Lee MY,Pui NN

    更新日期:2009-08-01 00:00:00

  • The colonic response to genotoxic carcinogens in the rat: regulation by dietary fibre.

    abstract::The apoptotic response to DNA damage appears to be an innate biological mechanism for protection against tumourigenesis. It is possible that agents that protect against colorectal cancer act by enhancing the apoptotic deletion of cells suffering DNA damage, with consequent removal of those with tumourigenic mutations....


    pub_type: 杂志文章


    authors: Hu Y,Martin J,Le Leu R,Young GP

    更新日期:2002-07-01 00:00:00

  • Modulation of arachidonic acid metabolism and nitric oxide synthesis by garcinol and its derivatives.

    abstract::Garcinol, a polyisoprenylated benzophenone, from the fruit rind of Garcinia spp., has been shown to have anti-inflammatory and anticarcinogenic activities. To study its mechanism of action, we analyzed the effects of garcinol and its derivatives, including cambogin, garcim-1 and garcim-2, on arachidonic acid metabolis...


    pub_type: 杂志文章


    authors: Hong J,Sang S,Park HJ,Kwon SJ,Suh N,Huang MT,Ho CT,Yang CS

    更新日期:2006-02-01 00:00:00

  • Induction of apoptosis in the lung but not in the liver of rats receiving intra-tracheal instillations of chromium(VI).

    abstract::Several studies have shown that hexavalent chromium [Cr(VI)] induces apoptosis in a variety of in vitro test systems. We instilled intra-tracheally either saline or sodium dichromate (0.25 mg/kg body weight), for three consecutive days, to Sprague-Dawley rats. TUNEL analyses showed a marked increase of the apoptotic i...


    pub_type: 杂志文章


    authors: D'Agostini F,Izzotti A,Bennicelli C,Camoirano A,Tampa E,De Flora S

    更新日期:2002-04-01 00:00:00

  • An insertion/deletion polymorphism at miRNA-122-binding site in the interleukin-1alpha 3' untranslated region confers risk for hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is the fifth most common malignancy caused by environmental and genetic factors. MicroRNAs (miRNAs) are a class of short non-coding RNAs with posttranscriptional regulatory functions. They participate in diverse biological pathways and function as gene regulators. Genetic polymorphisms i...


    pub_type: 杂志文章


    authors: Gao Y,He Y,Ding J,Wu K,Hu B,Liu Y,Wu Y,Guo B,Shen Y,Landi D,Landi S,Zhou Y,Liu H

    更新日期:2009-12-01 00:00:00

  • The metabolism and activation of polycyclic aromatic hydrocarbons in epithelial cell aggregates and fibroblasts prepared from rat mammary tissue.

    abstract::The metabolism of 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BP) and benz[a]anthracene (BA) by epithelial cell aggregates and fibroblasts in culture has been investigated using mammary tissue obtained from female Wistar rats. The results show that: (a) both types of mammary cells metabolise all three hydro...


    pub_type: 杂志文章


    authors: Cooper CS,Pal K,Hewer A,Grover PL,Sims P

    更新日期:1982-01-01 00:00:00

  • RSK2 as a key regulator in human skin cancer.

    abstract::Our previous report demonstrated that RSK2 plays an important role in cell proliferation and transformation induced by tumor promoters such as epidermal growth factor mediated through the N-terminal kinase domain of RSK2 in JB6 Cl41 mouse skin epidermal cells in vitro. However, no direct evidence has been reported reg...


    pub_type: 杂志文章


    authors: Cho YY,Lee MH,Lee CJ,Yao K,Lee HS,Bode AM,Dong Z

    更新日期:2012-12-01 00:00:00

  • CRK7 modifies the MAPK pathway and influences the response to endocrine therapy.

    abstract::Endocrine therapies, which inhibit estrogen receptor (ER)alpha signaling, are the most common and effective treatment for ERalpha-positive breast cancer. However, the use of these agents is limited by the frequent development of resistance. The cyclin-dependent kinase family member CRK7 (aka CRKRS) was identified from...


    pub_type: 杂志文章


    authors: Iorns E,Martens-de Kemp SR,Lord CJ,Ashworth A

    更新日期:2009-10-01 00:00:00

  • DNA--protein crosslinks, a biomarker of exposure to formaldehyde--in vitro and in vivo studies.

    abstract::Formaldehyde (FA) is a widely produced industrial chemical. Sufficient evidence exists to consider FA as an animal carcinogen. In humans the evidence is not conclusive. DNA-protein crosslinks (DPC) may be one of the early lesions in the carcinogenesis process in cells following exposures to carcinogens. It has been sh...


    pub_type: 杂志文章


    authors: Shaham J,Bomstein Y,Meltzer A,Kaufman Z,Palma E,Ribak J

    更新日期:1996-01-01 00:00:00

  • Mutagenicity of nitrosated alpha-amino acid derivatives N-acetyl-N'-nitrosotryptophan and its methyl ester in bacteria.

    abstract::DL-N-acetyl-N'-nitrosotryptophan (I) and its methyl ester (II), readily formed under mild conditions by the reaction of nitrite with N-acetyltryptophan or its methyl ester, are model compounds for the study of the nitrosation of alpha-amino acid side chains, considered relevant to possible role of nitrosation of pepti...


    pub_type: 杂志文章


    authors: Venitt S,Crofton-Sleigh C,Ooi SL,Bonnett R

    更新日期:1980-06-01 00:00:00

  • GSTM1 and CYP2D6 genotype frequencies in patients with pituitary tumours: effects on P53, ras and gsp.

    abstract::We describe studies to determine if susceptibility to pituitary tumours is associated with the putatively high risk GSTM1 null and CYP2D6 EM genotypes. Frequency distributions of these genotypes were similar in cases and controls though the frequency of CYP2D6 PM and GSTM1 B tended to be lower (P = 0.072 and P = 0.095...


    pub_type: 杂志文章


    authors: Perrett CW,Clayton RN,Pistorello M,Boscaro M,Scanarini M,Bates AS,Buckley N,Jones P,Fryer AA,Gilford J

    更新日期:1995-07-01 00:00:00

  • Discrimination of mutagenic intermediates derived from alkylating agents by mutational patterns generated in Escherichia coli.

    abstract::Reactive intermediates (ultimate mutagens/carcinogens) generated by alkylating agents are unstable and difficult to characterize by chemical means. We have used a genetic system to distinguish the in vivo interactions of eight carcinogenic methylating agents and five ethylating agents by the patterns of induced mutati...


    pub_type: 杂志文章


    authors: Elespuru RK,Stupar LL,Gordon JA

    更新日期:1991-07-01 00:00:00

  • The functional genetic variant Arg324Gly of frizzled-related protein is associated with colorectal cancer risk.

    abstract::The Wnt-beta-catenin pathway plays a central role in colorectal tumorigenesis. Frizzled-related protein (FRZB, also termed secreted frizzled-related protein 3, sFRP3) antagonizes the signaling of wingless (Wnt) ligands through the frizzled membrane-bound receptors, resulting in beta-catenin destabilization thereby sup...


    pub_type: 杂志文章


    authors: Shanmugam KS,Brenner H,Hoffmeister M,Chang-Claude J,Burwinkel B

    更新日期:2007-09-01 00:00:00

  • T cell status influences colon tumor occurrence in min mice fed short chain fructo-oligosaccharides as a diet supplement.

    abstract::We have previously shown that addition of short chain fructo-oligosaccharides (indigestible carbohydrates) to food prevented colon tumors in C57BL/6-Apc(Min/+) mice, a model for human colon cancer. As gut-associated lymphoid tissue was concomitantly developed, we suggested that the immune response generated by this fo...


    pub_type: 杂志文章


    authors: Pierre F,Perrin P,Bassonga E,Bornet F,Meflah K,Menanteau J

    更新日期:1999-10-01 00:00:00

  • Risk of genome-wide association study-identified genetic variants for non-Hodgkin lymphoma in a Chinese population.

    abstract::Recent genome-wide association studies have identified 15 single nucleotide polymorphisms (SNPs) associated with non-Hodgkin lymphoma (NHL) and its subtypes. Because the incidence and subtype portion of NHL between the Chinese population and Caucasian populations are substantially different, we assessed the associatio...


    pub_type: 杂志文章


    authors: Qiao Y,Zhou Y,Wu C,Zhai K,Han X,Chen J,Tian X,Chang J,Lu Z,Zhang B,Yu D,Yao J,Shi Y,Tan W,Lin D

    更新日期:2013-07-01 00:00:00

  • Cyclin D1 gene polymorphism is associated with an increased risk of urinary bladder cancer.

    abstract::Cyclin D1 is believed to play an important role in the genesis and/or progression of transitional cell cancer (TCC) of the urinary bladder. Cyclin D1 gene (CCND1) mRNA is alternatively spliced to produce two transcripts, and the splicing pattern may be modulated by a G to A single nucleotide polymorphism within the sp...


    pub_type: 杂志文章


    authors: Wang L,Habuchi T,Takahashi T,Mitsumori K,Kamoto T,Kakehi Y,Kakinuma H,Sato K,Nakamura A,Ogawa O,Kato T

    更新日期:2002-02-01 00:00:00

  • Expression of the glutathione S-transferase placental form in human lung carcinomas.

    abstract::Expression of glutathione S-transferase placental form (GST-pi) in human lung carcinoma tissue taken at autopsy or biopsy was investigated immunohistochemically. All of 34 cases of squamous cell carcinomas, including poorly, moderately and well-differentiated examples were shown to stain positively for GST-pi. Poorly ...


    pub_type: 杂志文章


    authors: Eimoto H,Tsutsumi M,Nakajima A,Yamamoto K,Takashima Y,Maruyama H,Konishi Y

    更新日期:1988-12-01 00:00:00

  • Azido- and nitro-PhIP, relatives of the heterocyclic arylamine and food mutagen PhIP--mechanism of their mutagenicity in Salmonella.

    abstract::Azido-PhIP (2-azido-1-methyl-6-phenylimidazo[4,5-b]pyridine) and nitro-PhIP (2-nitro-1-methyl-6-phenylimidazo[4,5-b]pyridine) have been synthesized and characterized chemically. The mutagenic potencies of azido-PhIP (with photoactivation by near UV irradiation), of nitro-PhIP (without exogenous activation) and of the ...


    pub_type: 杂志文章


    authors: Wild D,Watkins BE,Vanderlaan M

    更新日期:1991-06-01 00:00:00

  • Macromolecular binding of the thyroid carcinogen 3-amino-1,2,4-triazole (amitrole) catalyzed by prostaglandin H synthase, lactoperoxidase and thyroid peroxidase.

    abstract::3-Amino-1,2,4-triazole, a thyroid carcinogen and goitrogen, is negative in a wide variety of short-term mutagenicity assays. However, amitrole induces gene mutations and morphological transformation in Syrian hamster embryo fibroblasts, cells known to carry out the prostaglandin H synthase (PHS)-mediated peroxidative ...


    pub_type: 杂志文章


    authors: Krauss RS,Eling TE

    更新日期:1987-05-01 00:00:00

  • Chemopreventive sphingadienes downregulate Wnt signaling via a PP2A/Akt/GSK3β pathway in colon cancer.

    abstract::Sphingadienes (SDs) derived from soy and other natural sphingolipids are cytotoxic to colon cancer cells via an Akt-dependent mechanism and reduce adenoma formation in Apc(Min/+) mice. Wnt signaling is fundamental to colon carcinogenesis and is the basis for spontaneous tumorigenesis in Apc(Min/+) mice and patients wi...


    pub_type: 杂志文章


    authors: Kumar A,Pandurangan AK,Lu F,Fyrst H,Zhang M,Byun HS,Bittman R,Saba JD

    更新日期:2012-09-01 00:00:00