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Properties of the sarcoplasmic reticulum Ca(2+)-pump in coronary artery skinned smooth muscle.

Abstract：

:Pig coronary artery cultured smooth muscle cells were skinned using saponin. In the presence of an ATP-regenerating system and oxalate, the skinned cells showed an ATP-dependent azide insensitive Ca(2+)-uptake which increased linearly with time for > 1 h. The Ca(2+)-uptake occurred with Km values of 0.20 +/- 0.03 microM for Ca2+ and 400 +/- 34 microM for MgATP2-. Thapsigargin and cyclopiazonic acid inhibited this uptake with IC50 values of 0.13 +/- 0.02 and 0.56 +/- 0.04 microM, respectively. These properties of SR Ca(2+)-pump are similar to those reported for membrane fractions isolated from fresh smooth muscle of coronary artery and other arteries. However, optimum pH of the uptake in the skinned cells (6.2) was lower than that reported previously using isolated membranes (6.4-6.8).

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Elmoselhi AB,Blennerhassett M,Samson SE,Grover AK

doi

10.1007/BF01322337

subject

Has Abstract

pub_date

1995-10-18 00:00:00

pages

149-55

issue

2

eissn

0300-8177

issn

1573-4919

journal_volume

151

pub_type

杂志文章

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