Abstract：
:Purification methods of deoxyribonucleic acid (DNA) from degraded and contaminated forensic samples were investigated for polymerase chain reaction (PCR) analysis. DNA extracted from putrefied tissue or bloodstains sometimes contained the copurified contaminant, that was identified as the porphyrin compound (hematin). When contaminated but less degraded DNA was analyzed by PCR, it was necessary to eliminate the impurity by anion exchange column chromatography or chelating resin preparation, and ultrafiltration using Centricon microconcentrators. When highly degraded DNA was analyzed, trace amounts of high molecular weight DNA was recovered by electroelution method, and then further purified by both column chromatography and ultrafiltration. From thus purified samples, the amelogenin gene for sex determination could be amplified by dual PCR technique.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Akane A,Shiono H,Matsubara K,Nakamura H,Hasegawa M,Kagawa Msubject
Has Abstractpub_date
1993-05-01 00:00:00pages
691-701issue
3eissn
0022-1198issn
1556-4029journal_volume
38pub_type
杂志文章abstract：:A sensitive and reliable method for extraction and quantification of benzoylecgonine (BZE) and cocaine (COC) in urine is presented. Propyl-chloroformate was used as derivatizing agent, and it was directly added to the urine sample: the propyl derivative and COC were then recovered by liquid-liquid extraction procedure...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12880
更新日期：2015-09-01 00:00:00
abstract：:Unsupported excised skin may shrink by as much as 50% or more. In 1981, a method was developed for ring adhesion to skin with the goal of minimizing tissue distortion upon excision. Five modified versions of the technique bearing the author's name followed (Dorion types I, II, III, IV, and V). The scientific literatur...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01604.x
更新日期：2011-01-01 00:00:00
abstract：:Suicides due to fall from height in Geneva from 1991 to 2000 were reviewed. Scene investigations, autopsy findings, psychiatric histories, and toxicology results were examined. There were 197 of these suicides, an incidence of five cases per 100,000 inhabitants per year or one fourth of all suicides in Geneva per year...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Using postmortem multislice computed tomography (MSCT) and magnetic resonance imaging (MRI), 40 forensic cases were examined and findings were verified by subsequent autopsy. Results were classified as follows: (I) cause of death, (II) relevant traumatological and pathological findings, (III) vital reactions, (IV) rec...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:Accurate sorting of commingled human remains comprises a fundamental requirement for all further anthropological analyses. The lower limb bones are particularly important for reconstructing biological profiles. This study introduces a metric technique for sorting these elements using eight standard anthropological mea...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13884
更新日期：2019-03-01 00:00:00
abstract：:A sensitive, specific and simple color test for the improvised explosive urea nitrate is described. It is based on the formation of a red pigment upon the reaction between urea nitrate and p-dimethylaminocinnamaldehyde (p-DMAC) under neutral conditions. Urea itself, which is the starting material for urea nitrate, doe...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:Compositional analysis of the organic additives in smokeless handgun powder can provide forensic information to associate known and questioned samples. A reliable method for the quantitative extraction of smokeless powder additives would strengthen these measurements. To achieve quantitative recovery, both supercritic...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:Intraosseous T1-weighted (T1W) and short-tau inversion recovery (STIR) magnetic resonance imaging (MRI) signal intensity changes-so-called bone marrow edema-may be able to differentiate antemortem and postmortem fractures in human forensic imaging. The primary objective of this study was to investigate this hypothesis...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14475
更新日期：2020-09-01 00:00:00
abstract：:Illegal trade in snake parts has increased enormously. In spite of strict protection under wildlife act, a large number of snakes are being killed ruthlessly in India for venom and skin. Here, an interesting case involving confiscation of crystallized dried snake venom and subsequent DNA-based species identification i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.02049.x
更新日期：2012-05-01 00:00:00
abstract：:Using high sensitivity forensic STR polymerase chain reaction (PCR) typing procedures, we have found low concentrations of DNA contamination in plasticware and water assumed to be sterile, which is not detected by standard DNA procedures. One technique commonly used to eliminate the presence of DNA is ultraviolet (UV)...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00172.x
更新日期：2006-07-01 00:00:00
abstract：:A fatality in a teenager from the recreational intranasal abuse of methylphenidate (Ritalin) is reported. The prescribed use of methylphenidate (Ritalin) in the treatment of attention deficit and/or hyperactivity disorder is widespread. The intranasal abuse of methylphenidate (Ritalin) among teenagers is becoming incr...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The Texas Legislature in 1993 mandated a quarterly reporting requirement for hospitals and physicians performing electroconvulsive therapy (ECT) in the state (United States Government hospitals were excluded). The Texas Department of Mental Health and Mental Retardation (TDMHMR) was designated as the agency responsibl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:With increasing frequency, forensic psychiatrists are called upon to evaluate sex offenders for the courts and criminal justice system. While many clinicians have observed that denial of paraphilia is common in sex offenders, few studies have examined whether this population has severe psychopathology other than parap...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Owing to the scanty evidence on usefulness of information of appearance of gray hair for age estimation, this study was conducted to estimate age based on the appearance of gray hair on different body sites in a sample of autopsy cases in Sri Lanka. A descriptive cross-sectional study was conducted in Teaching Hospita...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13375
更新日期：2017-07-01 00:00:00
abstract：:The U.S. agricultural sector is vulnerable to intentionally introduced microbial threats because of its wide and open distribution and economic importance. To investigate such events, forensically valid assays for plant pathogen detection are needed. In this work, real-time PCR assays were developed for three model pl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12321
更新日期：2014-03-01 00:00:00
abstract：:A case of apparent suicide by injection of snake venom was investigated. Evidence found at the scene and presumably used for self-injection was positive for nonspecific venom but had the characteristics of a viper venom. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:High-energy synchrotron radiation x-ray fluorescence spectrometry (SR-XRF) utilizing 116 keV x-rays was used to characterize titanium dioxide pigments (rutile) and automotive white paint fragments for forensic examination. The technique allowed analysis of K lines of 9 trace elements in 18 titanium dioxide pigments (r...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01005.x
更新日期：2009-05-01 00:00:00
abstract：:We describe three unusual cases of suicide committed by health care workers. The aim of this paper was to analyze and evaluate the evidence of general diagnostic elements of poisoning in these cases. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Short tandem repeat (STR) loci represent a rich source of highly polymorphic markers in the human genome which are useful for the purposes of forensic identification and determination of biological relatedness of individuals. Here, as a part of an ongoing extensive study, we report the analysis of a multilocus genotyp...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Zolpidem is a sedative that could be used to drug victims, but its suitability to dissolve in drinks is unknown. In this small study, we added either crushed or whole tablets of zolpidem hemitartrate to carbonated beverages or still water to observe how this affected the taste and appearance. Also, concentrations were...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13604
更新日期：2018-05-01 00:00:00
abstract：:The main disadvantage to gunshot residue (GSR) particle analysis utilizing scanning electron microscope/energy dispersive X-ray (SEM/EDX) instrumentation has been the excessive operator time required for search and identification. This study uses an automated particle search and characterization program for unattended...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:New procedures, tailored after such court decisions as Rogers v. Commissioner of the Department of Mental Health, have restricted the doctor's ability to treat psychiatric inpatients with psychotropic medication and have increased the protection of a competent patient's right to refuse. This study investigates how the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:From the works of Broca and Krogman to modern-day Jantz and Buikstra, the orbit has been used for both quantitative and qualitative sex and race estimation. This study evaluates the practical value of these estimations. Orbital height and breadth were measured to determine the orbital index and assess differences betw...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01864.x
更新日期：2011-11-01 00:00:00
abstract：:Estimation of the time since death (postmortem interval [PMI]) is one of the most difficult problems in forensic investigations, and many methods currently are utilized to estimate the PMI. The goal of this study was to investigate the changes of attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectr...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.02036.x
更新日期：2012-05-01 00:00:00
abstract：:Diphenidine is a dissociative drug that shows several psychotropic effects including euphoria, shifts in perception of reality, hallucinations, and transient anterograde amnesia. In this study, a case of acute intoxication occurring after diphenidine intake is reported. A 30-year-old Caucasian male was hospitalized af...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.13355
更新日期：2017-07-01 00:00:00
abstract：:A wide variety of problems may prevent or hinder a dental-identification (ID) team in its efforts to identify mass casualties. Since these problems have been infrequently reported in a comprehensive manner, the authors identified and summarized these problems to increase the awareness of dental-ID team members and to ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:The Johns Hopkins University Center for Craniofacial Development and Disorders estimates that 1 in 3,000 children born in the United States is diagnosed with a rare form of craniosynostosis. Although the medical literature has documented numerous descriptions of craniofacial disorders from an anthropometric or genetic...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00872.x
更新日期：2008-11-01 00:00:00
abstract：:A 5-year study of a busy ski population in Utah revealed a total at-risk period of approximately 3.5 million skier-days. While the morbidity (fractures, strains, sprains, and occasionally more severe injuries) associated with skiing is well recognized and considered by most physicians concerned to be distributed somew...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Since patients with post-traumatic, neurologic, movement disorders often seek legal counsel and become involved in litigation, we used this group of disorders as a model for testing the hypothesis that an interaction with the legal system may influence its outcome. We reviewed 100 consecutive medical records of patien...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Improvements have been made in identifying the prevalence of risk factors/indicators for violent extremism. A consistent problem is the lack of base rates. How to develop base rates is of equal concern. This study has two aims: (i) compare two methods for developing base rates; the Unmatched Count Technique (UCT) and ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14282
更新日期：2020-05-01 00:00:00
