Abstract：
:The regional distribution of inositol 1,4,5-trisphosphate (InsP3), inositol 1,3,4,5-tetrakisphosphate (InsP4), and ryanodine binding sites has been characterised and compared in the rat brain using radioligand binding assays. Cortical [3H]InsP3 binding indicated similar positional and stereospecificity as observed in other tissues, with 100-fold selectivity for InsP3 over InsP4. Similarly, high-affinity [32P]InsP4 binding also showed a high degree of positional specificity, with a 1,000-fold selectivity for InsP4 over InsP3. Initial characterisation of [3H]ryanodine binding to cortical membranes demonstrated that specific binding was highly dependent on high salt and micromolar Ca2+ concentrations and inhibited by Ca2+ levels of > 1 mM. [3H]-Ryanodine binding was also enhanced by beta, gamma-methylene-adenosine 5'-trisphosphate and caffeine and inhibited by magnesium and ruthenium red (Ki = 0.81 microM). However, dantrolene (300 microM) was ineffective on the binding. Therefore, although the results indicate a greater similarity to the binding properties of the Ca(2+)-induced Ca2+ release channel isoform present in skeletal, rather than cardiac, muscle, it does not appear to be identical. Detailed binding analysis of ryanodine and polyphosphate sites, with the exception of ruthenium red, indicated no interaction between binding sites. Ruthenium red markedly enhanced the binding of both [3H]InsP3 and [32P]InsP4, an effect most probably due to nonspecific complex formation. Regional binding of InP3, InsP4, and ryanodine in the rat brain was of similar affinity for each ligand in each area, but the density profile for each ligand was clearly different. The highest density of InsP3 sites was in the cerebellum, whereas the highest density of ryanodine sites was in the hippocampus.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Smith SM,Nahorski SRdoi
10.1111/j.1471-4159.1993.tb13382.xsubject
Has Abstractpub_date
1993-05-01 00:00:00pages
1605-14issue
5eissn
0022-3042issn
1471-4159journal_volume
60pub_type
杂志文章abstract：:The myelin-deficient (mld) mouse is an autosomal recessive mutant characterized by hypomyelination of the CNS due to reduced expression of the myelin basic protein (MBP) gene. In the mld mutant, the MBP gene is duplicated in tandem. One gene is intact, but a large portion is inverted upstream of the other copy, and it...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1991.tb08186.x
更新日期：1991-02-01 00:00:00
abstract：:In this study we show that embryonic neurite growth-promoting protein amphoterin binds to carboxylated N -glycans previously identified on mammalian endothelial cells. Since amphoterin is a ligand for the receptor for advanced glycation end products (RAGE), and the ligand-binding V-domain of the receptor contains two ...
journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1980.tb09000.x
更新日期：1980-12-01 00:00:00
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abstract：:The AP1 site within the tyrosine hydroxylase gene proximal promoter is essential for the response of the gene to numerous stimuli. Stimulation of this gene is often associated with induction of the AP1 transcription factor, c-Fos. However, many stimuli activate or induce multiple transcription factors that interact wi...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.0022-3042.2001.00692.x
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1991.tb06365.x
更新日期：1991-11-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1991.tb02110.x
更新日期：1991-07-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1988.tb01147.x
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journal_title：Journal of neurochemistry
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更新日期：1991-04-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：2008-08-01 00:00:00
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更新日期：2003-11-01 00:00:00
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更新日期：2005-07-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1983.tb11836.x
更新日期：1983-07-01 00:00:00
abstract：:The effect of stress mediators following the stress period and addition time is a controversial issue until now. Thus, we aim to clarify the differential effects of single restraint stress (SS) or repeated restraint stress (RS) on kainic acid (KA)-induced neuronal death especially as addressing not only the role of gl...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.2007.04865.x
更新日期：2007-11-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1046/j.1471-4159.1995.65062716.x
更新日期：1995-12-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1988.tb01161.x
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journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
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更新日期：2000-11-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：1997-08-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：2009-05-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1995.64062387.x
更新日期：1995-06-01 00:00:00
abstract：:Fragile X syndrome (FXS) is a developmental disorder caused by the loss of Fragile X Mental Retardation 1 (FMR1) gene function because of a CGG repeat expansion (> 200 repeats) in the gene. The molecular mechanism(s) linking loss of FMR1 function to the molecular pathology and cognitive/behavioral disability remain un...
journal_title：Journal of neurochemistry
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更新日期：2012-05-01 00:00:00
