Nuclear calmodulin-binding proteins in rat neurons.


:By using a 125I-calmodulin overlay assay, three major high-affinity calmodulin-binding proteins, showing apparent molecular masses of 135, 60, and 50 kDa, have been detected in purified nuclear fractions isolated from rat neurons. It has been shown that after extraction of the nuclei with nucleases and high salt, all these proteins remain strongly associated with the nuclear matrix. The 60- and 50-kDa proteins have been previously identified as subunits of the calmodulin-dependent protein kinase II. We report here the immunoblot identification of the 135-kDa calmodulin-binding protein as myosin light chain kinase. We also show that the calmodulin-dependent protein phosphatase calcineurin is present in the neuronal nuclei and associated with the nuclear matrix. The nuclear localization of both calcineurin and myosin light chain kinase has been confirmed by immunocytochemical studies.


J Neurochem


Pujol MJ,Bosser R,Vendrell M,Serratosa J,Bachs O




Has Abstract


1993-04-01 00:00:00












  • Autoradiographic localization of dopaminergic and noradrenergic receptors in the bovine pineal gland.

    abstract::Dopamine and norepinephrine are involved in regulation of melatonin synthesis in the pineal gland. In bovine pineal gland, D1- and D2-dopaminergic and alpha 1-adrenergic receptors have been characterized pharmacologically in several laboratories, while beta 1-adrenergic receptors have been studied using physiological ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Simonneaux V,Happe HK,Ebadi M,Murrin LC

    更新日期:1991-11-01 00:00:00

  • Further characterization of an enkephalin-generating enzyme from adrenal medullary chromaffin granules.

    abstract::An adrenomedullary protease capable of generating Met5-enkephalin from endogenous precursor(s) has been purified 1,000-fold using affinity chromatography in combination with gel filtration. This trypsin-like enzyme has an apparent molecular weight of 20,000 daltons by gel filtration. The reactivity of the enzyme towar...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lindberg I,Yang HY,Costa E

    更新日期:1984-05-01 00:00:00

  • Vesicular storage and secretion of L-glutamate from glucagon-like peptide 1-secreting clonal intestinal L cells.

    abstract::Vesicular glutamate transporter (VGLUT) is responsible for the vesicular storage of l-glutamate, and plays an essential role in glutamate-mediated intercellular signal transmission in the CNS and in some neuroendocrine cells. Intestinal L cells are the glucose-responsive neuroendocrine cells responsible for the secret...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Uehara S,Jung SK,Morimoto R,Arioka S,Miyaji T,Juge N,Hiasa M,Shimizu K,Ishimura A,Otsuka M,Yamamoto A,Maechler P,Moriyama Y

    更新日期:2006-01-01 00:00:00

  • Proteasome inhibitor-induced apoptosis of glioma cells involves the processing of multiple caspases and cytochrome c release.

    abstract::The proteasome is a multiprotein complex that is involved in the intracellular protein degradation in eukaryotes. Here, we show that human malignant glioma cells are susceptible to apoptotic cell death induced by the proteasome inhibitors, MG132 and lactacystin. The execution of the apoptotic death program involves th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wagenknecht B,Hermisson M,Groscurth P,Liston P,Krammer PH,Weller M

    更新日期:2000-12-01 00:00:00

  • Glutamate-dependent transcriptional regulation of GLAST/EAAT1: a role for YY1.

    abstract::Glutamate is the major excitatory transmitter in the vertebrate brain and its extracellular levels are tightly regulated to prevent excitotoxic effects. The Na(+)-dependent glutamate/aspartate transporter GLAST/EAAT1 is regulated in the short and in the long term by glutamate. A receptors-independent change in its mem...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rosas S,Vargas MA,López-Bayghen E,Ortega A

    更新日期:2007-05-01 00:00:00

  • Manipulation of disulfide bonds differentially affects the intracellular transport, sorting, and processing of neuroendocrine secretory proteins.

    abstract::To investigate if the prevention of disulfide bond formation affects the intracellular transport, sorting, and processing of a distinct set of neuroendocrine proteins in the regulated secretory pathway, we have treated Xenopus intermediate pituitaries with the thiol-reducing agent dithiothreitol. Pulse-chase incubatio...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Van Horssen AM,Van Kuppeveld FJ,Martens GJ

    更新日期:1998-07-01 00:00:00

  • Influence on 5-[3H]hydroxytryptamine binding site development in chick embryo by serotonergic compounds.

    abstract::Saturable and specific binding sites for 5-[3H]hydroxytryptamine (5-HT, serotonin) characterized by a KD of 3.5-4.5 nM were detected in the chick embryo brain and were shown to develop linearly as a function of age, weight, and protein content. Saturation and displacement studies using unlabeled 5-HT as the displacing...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Soblosky JS,Jeng I

    更新日期:1985-02-01 00:00:00

  • Real-time monitoring of electrically stimulated norepinephrine release in rat thalamus: I. Resolution of transmitter and metabolite signal components.

    abstract::Electrical stimulation of an ascending path of the locus ceruleus-norepinephrine system was used to elicit release of norepinephrine at noradrenergic terminal fields of the rat thalamus. Overflow into the extracellular fluid space was measured by fast in vivo chronoamperometry. At pretreated carbon fibers, the electro...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ghasemzadeh MB,Capella P,Mitchell K,Adams RN

    更新日期:1993-02-01 00:00:00

  • Changes in lipogenic capacity and activities of ketolytic and lipogenic enzymes in brain regions of developing rats.

    abstract::Oxidation of ketone bodies (KBs) generates acetyl coenzyme A (AcCoA), which can be further incorporated into fatty acid. We have determined the rates of lipogenesis from ketone bodies in developing rats and their relation to the activities of enzymes involved in the production of cytoplasmic AcCoA via different pathwa...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yeh YY,Ginsburg JR,Tso TB

    更新日期:1983-01-01 00:00:00

  • Phosphorylation of neuronal kinesin heavy and light chains in vivo.

    abstract::The microtubule-based motor protein kinesin is thought to drive anterograde organelle transport in axons, but nothing is known about how its force-generating activity or organelle-binding properties are regulated. Studies in other motility systems suggest that protein phosphorylation is a reasonable candidate for this...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hollenbeck PJ

    更新日期:1993-06-01 00:00:00

  • A monoclonal antibody raised against plasma membrane of cholinergic nerve terminals of the Torpedo inhibits choline acetyltransferase activity and acetylcholine release.

    abstract::Monoclonal antibodies were raised against the synaptosomal plasma membranes (SPMs) purified from the electric organ of the Torpedo. One antibody that reacts preferentially with SPMs rather than with other membrane fractions isolated from this tissue was previously found to inhibit hydrophilic and amphiphilic choline-O...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Eder-Colli L,Briand PA,Pellegrinelli N,Dunant Y

    更新日期:1989-11-01 00:00:00

  • The malleable brain - An educational review from students to students.

    abstract::The Review "The malleable brain: plasticity of neural circuits and behavior - A review from students to students" has been written by a group of students following the International Society for Neurochemistry and Journal of Neurochemistry Flagship School. Comprehensively written by students for students, it provides b...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schulz JB,Hausmann L

    更新日期:2017-09-01 00:00:00

  • Partial isolation of two classes of dopamine beta-hydroxylase-containing particles undergoing rapid axonal transport in rat sciatic nerve.

    abstract::The rapid bidirectional transport of dopamine beta-hydroxylase (DBH) in adrenergic axons provides a means of analyzing the life cycle of adrenergic storage vesicles. We compared the physical characteristics of DBH-containing particles traveling to or returning from the terminal varicosities of ligated rat sciatic nerv...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Studelska DR,Brimijoin S

    更新日期:1989-08-01 00:00:00

  • Localisation of microtubule-associated protein 1B phosphorylation sites recognised by monoclonal antibody SMI-31.

    abstract::MAP 1B is a microtubule-associated phosphoprotein that is expressed early in neurons and plays a role in axon growth. MAP 1B has two types of phospho-isoforms, one of which is developmentally down-regulated after neuronal maturation and one of which persists into adulthood. Because phosphorylation regulates MAP 1B bin...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Johnstone M,Goold RG,Bei D,Fischer I,Gordon-Weeks PR

    更新日期:1997-10-01 00:00:00

  • A subtype of kappa-opioid receptor mediates inhibition of high-affinity GTPase inherent in Gi1 in guinea pig cerebellar membranes.

    abstract::The kappa-opioid receptor agonists including U-50,488H and dynorphin A (1-17) in ranges of 0.1-100 nM inhibited the hydrolysis of GTP to GDP (P(i) release) inherent in GTP-binding proteins (G proteins) in guinea pig cerebellar membranes. U-50,488H inhibited only high-affinity GTPase activity, not low-affinity activity...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ueda H,Misawa H,Fukushima N,Katada T,Ui M,Satoh M

    更新日期:1996-02-01 00:00:00

  • Reduced number of unmyelinated sensory axons in peripherin null mice.

    abstract::Peripherin is a type III intermediate filament (IF) abundantly expressed in developing neurons, but in the adult, it is primarily found in neurons extending to the peripheral nervous system. It has been suggested that peripherin may play a role in axonal elongation and/or cytoskeletal stabilization during development ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Larivière RC,Nguyen MD,Ribeiro-da-Silva A,Julien JP

    更新日期:2002-05-01 00:00:00

  • Tauroursodeoxycholic acid modulates p53-mediated apoptosis in Alzheimer's disease mutant neuroblastoma cells.

    abstract::Early onset familial Alzheimer's disease (FAD) is linked to autosomal dominant mutations in the amyloid precursor protein (APP) and presenilin 1 and 2 (PS1 and PS2) genes. These are critical mediators of total amyloid beta-peptide (Abeta) production, inducing cell death through uncertain mechanisms. Tauroursodeoxychol...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ramalho RM,Borralho PM,Castro RE,Solá S,Steer CJ,Rodrigues CM

    更新日期:2006-09-01 00:00:00

  • Sublytic terminal complement complexes decrease P0 Gene expression in Schwann cells.

    abstract::Complement cascade activation on peripheral nerve myelin can cause myelin destruction. Although terminal complement complexes (TCCs) are transiently detected on Schwann cells (SchCs) during inflammatory neuropathy, SchCs appear resistant to complement-mediated lysis, and little is known about the functional consequenc...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Dashiell SM,Koski CL

    更新日期:1999-12-01 00:00:00

  • Selective deficits in the expression of striatal-enriched mRNAs in Huntington's disease.

    abstract::We have identified and cataloged 54 genes that exhibit predominant expression in the striatum. Our hypothesis is that such mRNA molecules are likely to encode proteins that are preferentially associated with particular physiological processes intrinsic to striatal neurons, and therefore might contribute to the regiona...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Desplats PA,Kass KE,Gilmartin T,Stanwood GD,Woodward EL,Head SR,Sutcliffe JG,Thomas EA

    更新日期:2006-02-01 00:00:00

  • Characterization of N-[3H]methylcarbamylcholine binding sites and effect of N-methylcarbamylcholine on acetylcholine release in rat brain.

    abstract::The present experiments show that N-[3H]-methylcarbamylcholine ([3H]MCC) binds specifically and with high affinity to rat hippocampus, frontal cortex, and striatum. The highest maximal density of binding sites was apparent in frontal cortex and the lowest in hippocampus. [3H]MCC binding was potently inhibited by nicot...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Araujo DM,Lapchak PA,Collier B,Quirion R

    更新日期:1988-07-01 00:00:00

  • Pre-synaptic nicotinic and D receptors functionally interact on dopaminergic nerve endings of rat and mouse nucleus accumbens.

    abstract::The existence of pre-synaptic auto- and hetero receptors which modulate neurotransmitter release is well documented. Emerging evidence show that in some cases these pre-synaptic receptors may also cross-talk with each other. The aim of the present work was to investigate whether acetylcholine receptors (nAChRs) and do...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Grilli M,Zappettini S,Zoli M,Marchi M

    更新日期:2009-03-01 00:00:00

  • Amyloid beta peptide 1-40 enhances the action of Toll-like receptor-2 and -4 agonists but antagonizes Toll-like receptor-9-induced inflammation in primary mouse microglial cell cultures.

    abstract::The interaction of endogenous and exogenous stimulators of innate immunity was examined in primary cultures of mouse microglial cells and macrophages after application of defined Toll-like receptor (TLR) agonists [lipopolysaccharide (LPS) (TLR4), the synthetic lipopeptide Pam3Cys-Ser-Lys4 (Pam3Cys) (TLR2) and single-s...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lotz M,Ebert S,Esselmann H,Iliev AI,Prinz M,Wiazewicz N,Wiltfang J,Gerber J,Nau R

    更新日期:2005-07-01 00:00:00

  • Niemann-Pick type C disease: cellular pathology and pharmacotherapy.

    abstract::Niemann-Pick type C disease (NPCD) was first described in 1914 and affects approximately 1 in 150 000 live births. It is characterized clinically by diverse symptoms affecting liver, spleen, motor control, and brain; premature death invariably results. Its molecular origins were traced, as late as 1997, to a protein o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Wheeler S,Sillence DJ

    更新日期:2020-06-01 00:00:00

  • Gemfibrozil, food and drug administration-approved lipid-lowering drug, increases longevity in mouse model of late infantile neuronal ceroid lipofuscinosis.

    abstract::Late Infantile Neuronal Ceroid Lipofuscinosis (LINCL) is a rare neurodegenerative disease caused by mutations in the Cln2 gene that leads to deficiency or loss of function of the tripeptidyl peptidase 1 (TPP1) enzyme. TPP1 deficiency is known to cause the accumulation of autofluoroscent lipid-protein pigments in brain...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ghosh A,Rangasamy SB,Modi KK,Pahan K

    更新日期:2017-05-01 00:00:00

  • Role of protein kinase A in GABAA receptor dysfunction in CA1 pyramidal cells following chronic benzodiazepine treatment.

    abstract::One-week treatment with the benzodiazepine (BZ) flurazepam (FZP), results in anticonvulsant tolerance, associated with reduced GABAA receptor (GABAR) subunit protein and miniature inhibitory post-synaptic current (mIPSC) amplitude in CA1 neurons of rat hippocampus. Because protein kinase A (PKA) has been shown to modu...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lilly SM,Zeng XJ,Tietz EI

    更新日期:2003-05-01 00:00:00

  • Intracellular pH, lactate, and energy metabolism in neonatal brain during partial ischemia measured in vivo by 31P and 1H nuclear magnetic resonance spectroscopy.

    abstract::Sequential 31P and 1H nuclear magnetic resonance spectra were measured for neonatal piglets (n = 7) to determine the relationship between brain intracellular pH (pHi), lactate, and phosphorylated energy metabolites during partial ischemia. Simultaneous determinations of arterial and cerebral venous blood gases, pH, O2...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Corbett RJ,Laptook AR,Nunnally RL,Hassan A,Jackson J

    更新日期:1988-11-01 00:00:00

  • In vivo production and release of acetylcholine from primary fibroblasts genetically modified to express choline acetyltransferase.

    abstract::Primary rat fibroblasts genetically modified to express Drosophila choline acetyltransferase (dChAT) synthesize and release acetylcholine (ACh) in vitro. The ACh produced from the transduced fibroblasts was found to be enhanced by increasing amounts of choline chloride in the culture media. These dChAT-expressing cell...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fisher LJ,Schinstine M,Salvaterra P,Dekker AJ,Thal L,Gage FH

    更新日期:1993-10-01 00:00:00

  • Cellular quantification of tyrosine hydroxylase in the rat brain by immunoautoradiography.

    abstract::We developed a rapid and sensitive radioimmunohistochemical method for the quantification of tyrosine hydroxylase (TH) at both the anatomical and cellular level. Coronal tissue sections from fresh-frozen rat brains were incubated in the presence of a TH monoclonal antibody. The reaction was revealed with a 35S-labeled...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Blanchard V,Raisman-Vozari R,Savasta M,Hirsch E,Javoy-Agid F,Feuerstein C,Agid Y

    更新日期:1993-08-01 00:00:00

  • 5-HT stimulates eEF2 dephosphorylation in a rapamycin-sensitive manner in Aplysia neurites.

    abstract::In Aplysia, serotonin mediates behavioral sensitization by increasing the strength of the synapse between sensory and motor neurons, a process known as facilitation. The retention of long-term facilitation is blocked by rapamycin, an inhibitor of a specific translational pathway. One possible rapamycin-sensitive targe...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Carroll M,Warren O,Fan X,Sossin WS

    更新日期:2004-09-01 00:00:00

  • Effects of branched-chain L-amino acids, L-phenylalanine, and L-methionine on the transport of L-glutamine in rat brain cortex in vitro. Influence of cations.

    abstract::Uptake of L-glutamine (2 mM) by rat brain cortex slices against a concentration gradient is markedly inhibited (40%) by branched-chain L-amino acids (1 mM), L-phenylalanine (1 mM), or L-methionine (1 mM); that of L-asparagine (2 mM) is much less affected by these amino acids. Other amino acids investigated have little...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Benjamin AM,Verjee ZH,Quastel JH

    更新日期:1980-07-01 00:00:00