当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Crystallization of porcine liver ribonuclease inhibitor a member of the family of proteins containing leucine-rich repeats.

Crystallization of porcine liver ribonuclease inhibitor a member of the family of proteins containing leucine-rich repeats.

Abstract：

:Single crystals of the RNase inhibitor from porcine liver have been obtained from 30 to 34% saturated ammonium sulphate solutions at pH 6.0 to 7.2, containing 20 mM dithiothreitol, at room temperature over a period of two to three weeks. Because the inhibitor contains 30 1/2-cystinyl residues, all of which occur in the free thiol form, crystallization experiments were carried out in a desiccator under a nitrogen atmosphere. The crystals belong to the tetragonal space group I4, with cell dimensions a = b = 134.76 A and c = 83.65 A. The asymmetric part of the unit cell contains two molecules with a molecular mass of 49,093 Da, as could be shown with a self-rotation function calculated in the resolution range 10.0 to 3.2 A. The crystals diffract to at least 3.2 A resolution and are suitable for an X-ray structure determination.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Neumann U,Hofsteenge J,Arkema AH,Dijkstra BW

doi

10.1006/jmbi.1993.1299

subject

Has Abstract

pub_date

1993-05-20 00:00:00

pages

505-8

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)71299-4

journal_volume

231

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • The human telomeric protein TRF1 specifically recognizes nucleosomal binding sites and alters nucleosome structure.

    abstract：:Telomeres are dynamic nucleoprotein structures that cap the ends of eukaryotic chromosomes. In humans, the long (TTAGGG)(n) double-stranded telomeric DNA repeats are bound specifically by the two related proteins TRF1 and TRF2, and are organized in nucleosomes. Whereas the role of TRF1 and TRF2 in telomeric function h...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.04.071

    authors： Galati A,Rossetti L,Pisano S,Chapman L,Rhodes D,Savino M,Cacchione S

    更新日期：2006-07-07 00:00:00

  • Contribution of opening and bending dynamics to specific recognition of DNA damage.

    abstract：:Guanine-uracil (G.U) wobble base-pairs are a detrimental lesion in DNA. Previous investigations have shown that such wobble base-pairs are more prone to base-opening than the normal G.C base-pairs. To investigate the sequence-dependence of base-pair opening we have performed 5ns molecular dynamics simulations on G.U w...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00598-9

    authors： Seibert E,Ross JB,Osman R

    更新日期：2003-07-18 00:00:00

  • Protein-DNA interactions: amino acid conservation and the effects of mutations on binding specificity.

    abstract：:We investigate the conservation of amino acid residue sequences in 21 DNA-binding protein families and study the effects that mutations have on DNA-sequence recognition. The observations are best understood by assigning each protein family to one of three classes: (i) non-specific, where binding is independent of DNA ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00571-5

    authors： Luscombe NM,Thornton JM

    更新日期：2002-07-26 00:00:00

  • Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin.

    abstract：:alpha-Lactalbumin (alpha-LA) is a two-domain, calcium-binding protein that forms one of the best studied molten globules. We present here amide hydrogen exchange studies of the molten globule formed by human alpha-LA at pH 2 and compare these results with a similar study of the native state at pH 6.3. The most persist...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0579

    authors： Schulman BA,Redfield C,Peng ZY,Dobson CM,Kim PS

    更新日期：1995-11-10 00:00:00

  • Involvement of the domains of histones H1 and H5 in the structural organization of soluble chromatin.

    abstract：:We have studied in reconstitution experiments the conditions under which peptides derived from histones H1 and H5 are bound in chromatin and to what extent they are involved in the organization of chromatin fibers. The fragments of rat liver histone H1 (rH1) and chicken erythrocytes H1 (cH1) and H5 (cH5) used were the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(83)80102-8

    authors： Thoma F,Losa R,Koller T

    更新日期：1983-07-05 00:00:00

  • Molecular Highways-Navigating Collisions of DNA Motor Proteins.

    abstract：:Fundamental biological processes require concurrent sharing of DNA by numerous motor proteins and complexes. Thus, collision, congestion, and roadblocks are inescapable on these busy "molecular highways." The consequences of these traffic problems are diverse, resulting in complex cellular mechanisms to resolve threat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.08.006

    authors： Le TT,Wang MD

    更新日期：2018-10-26 00:00:00

  • Crystal structure of human cyclin K, a positive regulator of cyclin-dependent kinase 9.

    abstract：:Cyclin K and the closely related cyclins T1, T2a, and T2b interact with cyclin-dependent kinase 9 (CDK9) forming multiple nuclear complexes, referred to collectively as positive transcription elongation factor b (P-TEFb). Through phosphorylation of the C-terminal domain of the RNA polymerase II largest subunit, distin...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.11.057

    authors： Baek K,Brown RS,Birrane G,Ladias JA

    更新日期：2007-02-16 00:00:00

  • Telomeric Transcription and Telomere Rearrangements in Quiescent Cells.

    abstract：:Despite the condensed nature of terminal sequences, the telomeres are transcribed into a group of noncoding RNAs, including the TElomeric Repeat-containing RNA (TERRA). Since the discovery of TERRA, its evolutionary conserved function has been confirmed, and its involvement in telomere length regulation, heterochromat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2020.01.034

    authors： Coulon S,Vaurs M

    更新日期：2020-07-10 00:00:00

  • The chromatin of active genes is not in a permanently open conformation.

    abstract：:Quantitative measurements of local chromatin accessibility to DNase I in 15-day chicken embryo erythrocyte nuclei have been performed using a range of nuclease concentrations and real-time TaqMan PCR to monitor the loss of short ( approximately 80 bp) amplicons. At the beta-globin locus, well-established DNase I hyper...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.12.048

    authors： Sapojnikova N,Thorne A,Myers F,Staynov D,Crane-Robinson C

    更新日期：2009-02-20 00:00:00

  • Cation transport: an example of structural based selectivity.

    abstract：:Through the high-resolution structure of the gramicidin A channel in lamellar phase lipids and the characterization of specific ion peptide interactions, fundamental principles for ion channel selectivity and conductance efficiency are illustrated with atomic resolution detail. Delocalized cation binding in the first ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2434

    authors： Tian F,Cross TA

    更新日期：1999-02-05 00:00:00

  • Crystal structure of the soluble domain of the major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria: a new class of copper-containing nitrite reductases.

    abstract：:The major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria gonorrhoeae is essential for cell growth under oxygen limiting conditions in the presence of nitrite and is protective against killing by human sera. A phylogenic analysis indicates that AniA is a member of a new class of copper-co...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5251

    authors： Boulanger MJ,Murphy ME

    更新日期：2002-02-01 00:00:00

  • Characterization and mutational analysis of the RecQ core of the bloom syndrome protein.

    abstract：:Bloom syndrome protein forms an oligomeric ring structure and belongs to a group of DNA helicases showing extensive homology to the Escherichia coli DNA helicase RecQ, a suppressor of illegitimate recombination. After over-production in E.coli, we have purified the RecQ core of BLM consisting of the DEAH, RecQ-Ct and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00534-5

    authors： Janscak P,Garcia PL,Hamburger F,Makuta Y,Shiraishi K,Imai Y,Ikeda H,Bickle TA

    更新日期：2003-06-27 00:00:00

  • The conformational properties of the Glc3Man unit suggest conformational biasing within the chaperone-assisted glycoprotein folding pathway.

    abstract：:A major puzzle is: are all glycoproteins routed through the ER calnexin pathway irrespective of whether this is required for their correct folding? Calnexin recognizes the terminal Glcalpha1-3Manalpha linkage, formed by trimming of the Glcalpha1-2Glcalpha1-3Glcalpha1-3Manalpha (Glc3Man) unit in Glc3Man9GlcNAc2. Differ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.01.043

    authors： Mackeen MM,Almond A,Deschamps M,Cumpstey I,Fairbanks AJ,Tsang C,Rudd PM,Butters TD,Dwek RA,Wormald MR

    更新日期：2009-03-27 00:00:00

  • 2.5 A structure of aspartate carbamoyltransferase complexed with the bisubstrate analog N-(phosphonacetyl)-L-aspartate.

    abstract：:In an X-ray diffraction study using the method of multiple isomorphous replacement, the structure of aspartate carbamoyltransferase (EC 2.1.3.2) complexed with the bisubstrate analog N-(phosphonacetyl)-L-aspartate (PALA) has been solved to 2.5 A. Ten rounds of model building and 123 cycles of restrained reciprocal spa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90265-8

    authors： Krause KL,Volz KW,Lipscomb WN

    更新日期：1987-02-05 00:00:00

  • Dystrophin Dp71f associates with the beta1-integrin adhesion complex to modulate PC12 cell adhesion.

    abstract：:Dystrophin Dp71 is the main product of the Duchenne muscular dystrophy gene in the brain; however, its function is unknown. To study the role of Dp71 in neuronal cells, we previously generated by antisense treatment PC12 neuronal cell clones with decreased Dp71 expression (antisense-Dp71 cells). PC12 cells express two...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.07.075

    authors： Cerna J,Cerecedo D,Ortega A,García-Sierra F,Centeno F,Garrido E,Mornet D,Cisneros B

    更新日期：2006-10-06 00:00:00

  • The structure of an RNA "kissing" hairpin complex of the HIV TAR hairpin loop and its complement.

    abstract：:We have used nuclear magnetic resonance (NMR) to obtain the structure of an RNA "kissing" hairpin complex formed between the HIV-2 TAR hairpin loop and a hairpin with a complementary loop sequence. Kissing hairpins are important in natural antisense reactions; their complex is a specific target for protein binding. Th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1021

    authors： Chang KY,Tinoco I Jr

    更新日期：1997-05-30 00:00:00

  • Binding of Escherichia coli RNA polymerase to a promoter carrying mutations that stop transcription initiation.

    abstract：:The gal P2 promoter can be inactivated by point mutations located in the -10 hexamer sequence or immediately upstream from it. Mutations at either site reduce expression in vivo and prevent the formation, in vitro, of tight complexes with RNA polymerase that give a strong footprint and can initiate transcription. Howe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90194-x

    authors： Johnston F,Ponnambalam S,Busby S

    更新日期：1987-06-05 00:00:00

  • Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity.

    abstract：:Post-translational modifications are essential for the proper function of many proteins in the cell. The attachment of an isoprenoid lipid (a process termed prenylation) by protein farnesyltransferase (FTase) or geranylgeranyltransferase type I (GGTase-I) is essential for the function of many signal transduction prote...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.08.056

    authors： Reid TS,Terry KL,Casey PJ,Beese LS

    更新日期：2004-10-15 00:00:00

  • Identification of an epitope in the C terminus of normal prion protein whose expression is modulated by binding events in the N terminus.

    abstract：:We have characterized the epitopes of a panel of 12 monoclonal antibodies (Mabs) directed to normal human cellular prion protein (PrP(C)) using ELISA and Western blotting of recombinant PrP or synthetic peptide fragments of PrP. The first group of antibodies, which is represented by Mabs 5B2 and 8B4, reacts with PrP(2...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3986

    authors： Li R,Liu T,Wong BS,Pan T,Morillas M,Swietnicki W,O'Rourke K,Gambetti P,Surewicz WK,Sy MS

    更新日期：2000-08-18 00:00:00

  • ALS mutants of human superoxide dismutase form fibrous aggregates via framework destabilization.

    abstract：:Many point mutations in human Cu,Zn superoxide dismutase (SOD) cause familial amyotrophic lateral sclerosis (FALS), a fatal neurodegenerative disorder in heterozygotes. Here we show that these mutations cluster in protein regions influencing architectural integrity. Furthermore, crystal structures of SOD wild-type and...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00889-1

    authors： DiDonato M,Craig L,Huff ME,Thayer MM,Cardoso RM,Kassmann CJ,Lo TP,Bruns CK,Powers ET,Kelly JW,Getzoff ED,Tainer JA

    更新日期：2003-09-19 00:00:00

  • Decomposition of the free energy of a system in terms of specific interactions. Implications for theoretical and experimental studies.

    abstract：:Recently, a number of methods have been proposed that are designed to extract contributions to the change in free energy associated with a given perturbation or mutation of a protein originating from specific residue-residue or atom-atom interactions, both based on theoretical calculations and on experimental data. We...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1430

    authors： Mark AE,van Gunsteren WF

    更新日期：1994-07-08 00:00:00

  • Conformational changes during the assembly of factor B from its domains by (1)H NMR spectroscopy and molecular modelling: their relevance to the regulation of factor B activity.

    abstract：:Factor B is a key component of the alternative pathway of complement and is cleaved by factor D into the Ba and Bb fragments in the presence of activated C3 (C3b or C3(H(2)O)). The Ba fragment contains three short consensus/complement repeat domains, while the Bb fragment contains a von Willebrand factor type A (vWF-A...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4044

    authors： Hinshelwood J,Perkins SJ

    更新日期：2000-09-01 00:00:00

  • The three-dimensional structure of N-succinyldiaminopimelate aminotransferase from Mycobacterium tuberculosis.

    abstract：:Inhibitors of the enzymes of the lysine biosynthetic pathway are considered promising lead compounds for the design of new antibacterial drugs, because the pathway appears to be indispensable for bacteria and because it is absent in humans. As part of our efforts to structurally characterize all enzymes of this pathwa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.01.023

    authors： Weyand S,Kefala G,Weiss MS

    更新日期：2007-03-30 00:00:00

  • A measure for the angle between projections based on the extent of correlation between corresponding central sections.

    abstract：:A pre-condition for the ab initio assignment of Euler angles to a set of projections from an asymmetric object is that at least three of the available projections correspond to rotations about different axes. For symmetric objects this condition may be relaxed. There are some applications of single-particle electron m...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.09.068

    authors： Patwardhan A,Paul D,Al-Khayat HA,Morris EP

    更新日期：2004-11-26 00:00:00

  • Crystal structure of the Ly49I natural killer cell receptor reveals variability in dimerization mode within the Ly49 family.

    abstract：:Natural killer (NK) cells play a crucial role in the detection and destruction of virally infected and tumor cells during innate immune responses. The cytolytic activity of NK cells is regulated through a balance of inhibitory and stimulatory signals delivered by NK receptors that recognize classical major histocompat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00498-9

    authors： Dimasi N,Sawicki MW,Reineck LA,Li Y,Natarajan K,Margulies DH,Mariuzza RA

    更新日期：2002-07-12 00:00:00

  • Structural characterization of the Yersinia pestis type III secretion system needle protein YscF in complex with its heterodimeric chaperone YscE/YscG.

    abstract：:The plague-causing bacterium Yersinia pestis utilizes a type III secretion system to deliver effector proteins into mammalian cells where they interfere with signal transduction pathways that mediate phagocytosis and the inflammatory response. Effector proteins are injected through a hollow needle structure composed o...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.12.067

    authors： Sun P,Tropea JE,Austin BP,Cherry S,Waugh DS

    更新日期：2008-03-28 00:00:00

  • Characterization of long-range structure in the denatured state of staphylococcal nuclease. II. Distance restraints from paramagnetic relaxation and calculation of an ensemble of structures.

    abstract：:Structural analysis of delta131delta, a fragment model of the denatured state of staphylococcal nuclease, has been extended by obtaining long-range distance restraints between chain segments by paramagnetic relaxation enhancement. Fourteen unique PROXYL spin labels were introduced at sites that are solvent-exposed in ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.0953

    authors： Gillespie JR,Shortle D

    更新日期：1997-04-25 00:00:00

  • Crystallization and preliminary X-ray analysis of Gc, the vitamin D-binding protein in serum.

    abstract：:The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mM-sodium acetate at pH 4.8. These crystals diffract to 3.4 A and the observed diffraction is consistent with orthorhombic space groups P4...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90097-p

    authors： Vogelaar NJ,Lindberg U,Schutt CE

    更新日期：1991-08-05 00:00:00

  • Computational design of calmodulin mutants with up to 900-fold increase in binding specificity.

    abstract：:Calmodulin (CaM) is a ubiquitous second messenger protein that regulates a variety of structurally and functionally diverse targets in response to changes in Ca(2+) concentration. CaM-dependent protein kinase II (CaMKII) and calcineurin (CaN) are the prominent CaM targets that play an opposing role in many cellular fu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.09.053

    authors： Yosef E,Politi R,Choi MH,Shifman JM

    更新日期：2009-02-06 00:00:00

  • A novel method to detect functional microRNA targets.

    abstract：:MicroRNA (miRNA) molecules are non-coding RNAs, 19 to 24 nt in length that have been identified recently as important regulators of gene expression. Several computational methods have been developed to describe the target recognition mechanism by miRNA. We propose here a novel method to detect miRNA-mRNA complexes in ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.02.063

    authors： Vatolin S,Navaratne K,Weil RJ

    更新日期：2006-05-12 00:00:00