Multidose use of exametazine for leukocyte labelling: a new approach using tin enhancement.

Abstract:

:Reconstituted exametazime is inherently unstable due to the rapid oxidation of the small amount (7.6 micrograms) of stannous chloride present in the kit. A novel approach has been used to improve the shelf-life of reconstituted exametazime based on the premise that tin lost through oxidation should be replaced by further amounts of tin. Freshly prepared stannous solution, 0.1 ml (0.66 microgram Sn2+), was mixed with 0.3 ml (25 micrograms) exametazime solution followed by 400-500 MBq pertechnetate. Mixed leukocytes from 50 ml of patients' blood were labelled with 99Tcm-exametazime which had been prepared by the tin enhancement. One hundred and fourteen patients were investigated. The median labelling efficiency was 72%, range 30-96%. The exametazime had been reconstituted up to 5 months previously. Because of the small volumes of the exametazime solution used, up to 15 doses for leukocyte labelling could be prepared from one vial.

journal_name

Nucl Med Commun

authors

Solanki C,Li DJ,Wong A,Miles KA,Sampson CB

doi

10.1097/00006231-199311000-00016

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

1035-40

issue

11

eissn

0143-3636

issn

1473-5628

journal_volume

14

pub_type

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