Abstract:
:Testosterone, testosterone propionate, 17 beta-trenbolone and progesterone, which represent the main endogenous and synthetic androgens and a progestin, were evaluated for possible cell transformation and genetic effects in Syrian hamster embryo (SHE) cells. Cell growth was reduced by treatment with the steroids at 10-30 micrograms/ml in a dose-related manner. Testosterone and testosterone propionate were less toxic than the other two steroids. Testosterone, testosterone propionate and progesterone induced morphological transformation of SHE cells with similar transformation frequencies. The most potent effects were observed with testosterone propionate, which induced cell transformation at 1-30 micrograms/ml in a dose-related manner. Testosterone and progesterone transformed cells only at the highest dose (30 micrograms/ml). 17 beta-Trenbolone did not induce a statistically significant level of cell transformations at any dose tested (up to 30 micrograms/ml). The transformation frequencies induced by testosterone, testosterone propionate and progesterone were less than one-half that induced by benzo[a]pyrene at 1 microgram/ml. None of these steroids induced significant increases in frequencies of chromosome aberrations or aneuploidy. Gene mutations were not observed for testosterone at the HPRT or Na+/K+ ATPase locus. Because these steroids are also associated with carcinogenic activity in vivo, these in vitro findings provide a model and new insights into the study of the mechanisms of androgen- and progestin-induced cell transformation.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Tsutsui T,Komine A,Huff J,Barrett JCdoi
10.1093/carcin/16.6.1329subject
Has Abstractpub_date
1995-06-01 00:00:00pages
1329-33issue
6eissn
0143-3334issn
1460-2180journal_volume
16pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Purified DNA was modified in vitro by 3H-labelled O-acetyl or O,O'-diacetyl-4-hydroxyaminoquinoline-1-oxide (Ac4HAQO or di Ac-4HAQO). It was then subjected to the action of the single-stranded DNA specific nuclease S1 and the digested fractions were analysed. For both types of modified DNA, the release of non-modified...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.4.435
更新日期:1982-01-01 00:00:00
abstract::This commentary celebrates the 40th year of Carcinogenesis, spanning 1980-2020 with a focus on lung cancer. For lung cancer, these 40 years come toward the end of a century of scientific inquiry that began with descriptions of this highly fatal malignancy and that closes with emphasis on molecular processes and genomi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa094
更新日期:2020-10-15 00:00:00
abstract::Sequencing of p53 exons 5-8 was carried out on 51 initial superficial bladder tumors selected on the basis of high grade and/or p53 overexpression (immunohistochemistry without antigen retrieval). Fourteen point mutations in 13 tumors and one 21 bp deletion in another tumor were identified. In addition, a germ-line mu...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.1.101
更新日期:2000-01-01 00:00:00
abstract::To investigate the sensitivity of heterozygous p53-deficient CBA mice to carcinogens, 20 female mice [p53(+/-)] and 20 wild-type littermates [p53(+/+)] were given an intraperitoneal injection of 120 mg/kg body wt of N-ethyl-N-nitrosourea (ENU) and were maintained without any other treatment for a further 26 weeks. His...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.5.1039
更新日期:2000-05-01 00:00:00
abstract::The Tsc-2 tumor suppressor gene encodes the protein tuberin, a multi-functional protein with sequence homology to the GTPase activating protein (GAP) for Rap1. Mutations in the Tsc-2 gene are associated with the development of renal tumors. The Eker rat (Tsc-2(EK/+)) bears a mutation in one allele of the Tsc-2 gene, w...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.3.573
更新日期:2003-03-01 00:00:00
abstract::Mutant RAS (RAS(V12)) is known to transform most immortal cells but to induce premature senescence in primary cells. RAS(V12)-induced senescence in murine cells depends on the induction of the ARF/p53 and the retinoblastoma (Rb) family tumor suppressor pathways. We and others have shown previously that oncogene-induce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp296
更新日期:2010-02-01 00:00:00
abstract::The relationship between urinary metabolites and DNA damage in the urinary bladder epithelium of male and female rats was tested by alkaline elution assay after an intravesical injection of OPP or its urinary metabolites. 2-Phenyl-1,4-benzoquinone (PBQ) revealed a weak DNA-damaging activity in both sexes at 0.05-0.1%....
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.10.1823
更新日期:1989-10-01 00:00:00
abstract::S-Adenosyl-L-methionine (SAM) is a strong chemopreventive agent of rat liver carcinogenesis. Examination was made to determine whether inhibition by SAM of the development of preneoplastic liver lesions persists to SAM withdrawal in diethylnitrosamine-initiated F344 rats promoted with thiobenzamide (TB). The rats were...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.7.1533
更新日期:1996-07-01 00:00:00
abstract::The effect of changing the format of administration as well as the total dose of the promoting agent phenobarbital (PB) on the development of altered hepatic foci (AHF) was determined in an initiation-promotion protocol with female rats fed the purified AIN-76 diet. Effects on the total number of AHF and the volume pe...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.6.1009
更新日期:1991-06-01 00:00:00
abstract::Epidemiological investigations suggest that chronic lung inflammation increases lung cancer risk. Pharmacologic and genetic evidence in mouse models indicates that lipid mediators released during pulmonary inflammation enhance lung tumor formation. Cytosolic phospholipase A2 (cPLA2) catalyzes arachidonic acid (AA) rel...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh150
更新日期:2004-08-01 00:00:00
abstract::The hypolipidaemic drug nafenopin (NAF) has been shown to enhance the hepatocarcinogenic effect of N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine in rats. We have investigated whether the NAF-induced peroxisome proliferation in hepatocytes interferes with NDMA's metabolism and interaction with DNA. Adult male...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.9.1309
更新日期:1985-09-01 00:00:00
abstract::Naturally occurring allyl isothiocyanate (AITC) was recently shown to be selectively delivered to bladder cancer tissue via urinary excretion and to inhibit bladder cancer growth and muscle invasion in an animal model. AITC is excreted in urine mainly as N-acetyl-S-(N-allylthiocarbamoyl)cysteine, more commonly known a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr283
更新日期:2012-02-01 00:00:00
abstract::To elucidate the mechanism of metabolic activation of 3'-methyl-N,N-dimethyl-4-aminoazobenzene (3'-Me-DAB), the mutagenicity of its metabolites and some related azo compounds towards Salmonella typhimurium TA-98 and TA-100 was investigated. Mutagenicity of the metabolites, either N-demethylated or oxidized at the 3'-m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.2.121
更新日期:1980-02-01 00:00:00
abstract::An intrasanguineous host-mediated assay was used to establish the mutagenic potential of a series of dialkylnitrosamines to E. coli K12/343/113 in the liver and spleen of mice. For calibrating purposes, dose- and time-dependent kinetics of mutation induction by the model compound diethylnitrosamine in this assay was d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.9.909
更新日期:1981-01-01 00:00:00
abstract::Previous studies have shown that decorin expression is significantly reduced in colorectal cancer tissues and cancer cells, and genetic deletion of the decorin gene is sufficient to cause intestinal tumor formation in mice, resulting from a downregulation of p21, p27(kip1) and E-cadherin and an upregulation of β-caten...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr293
更新日期:2012-02-01 00:00:00
abstract::The possibility that ascorbic acid, as a nucleophile, may inhibit mutagenicity induced by electrophilic metabolites of N-nitroso compounds was examined. In vitro data are presented to show that ascorbic acid does not decrease the mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in a modified Ames bacterial ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.11.1593
更新日期:1985-11-01 00:00:00
abstract::The chemopreventive effects of phenethyl isothiocyanate (PEITC) were investigated in N-nitrosobis(2-oxopropyl)-amine (BOP)-treated hamsters. Female 5-week-old Syrian golden hamsters were divided into six groups. Animals in groups 1-3, each consisting of 30 hamsters, were given BOP by two subcutaneous injections 7 days...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.6.1381
更新日期:1996-06-01 00:00:00
abstract::Resveratrol is a phytochemical that has been under consideration for use as a prostate cancer chemopreventive agent. However, the efficacy, as well as the mechanisms of action of resveratrol on prostate cancer prevention, remains largely unknown. This study seeks to address these questions and examine the cancer preve...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn131
更新日期:2008-10-01 00:00:00
abstract::O6-Methylguanine-DNA methyltransferase (MT)-proficient Chinese hamster ovary cells were grown in the presence of low, gradually increasing levels of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) with the aim of selecting MNNG-resistant cell lines. Six resistant clones with two levels of resistance were isolated. A 3-fol...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.7.1217
更新日期:1988-07-01 00:00:00
abstract::The risk of squamous cell cancers of the oral cavity (OSCC) is strongly related to the use of tobacco and alcohol. N-Acetyl transferases 1 and 2 (NAT2) metabolize aryl- and heterocyclic amines that are present in tobacco smoke. NAT2 slow acetylator phenotype or genotype is related to reduced ability to detoxify these ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.12.1993
更新日期:2001-12-01 00:00:00
abstract::2-methoxyestradiol (2-MeOE(2)) is an endogenous metabolite of 17beta-estradiol and a proposed inhibitor of tumor growth and angiogenesis. However, 2-MeOE(2) is also an inhibitor of microtubule assembly and other microtubule inhibitors, e.g. colcemid and diethylstilbestrol, induce aneuploidy and cell transformation in ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.4.735
更新日期:2000-04-01 00:00:00
abstract::BALB/c 3T3 A31-1-13 cells are highly susceptible to chemical-induced cell transformation and their gap-junctional intercellular communication (GJIC) is decreased when these cells become confluent. On the other hand, A31-1-1 cells do not show such a change in GJIC and are more resistant to chemical induction of transfo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.10.1923
更新日期:1991-10-01 00:00:00
abstract::DNA double-strand break (DSB) repair is an important mechanism underlying chemotherapy resistance in human cancers. Dicer participates in DSB repair by facilitating homologous recombination. However, whether Dicer is involved in non-homologous end joining (NHEJ) remains unknown. Here, we addressed whether Dicer regula...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx059
更新日期:2017-09-01 00:00:00
abstract::Hydrazine sulfate is a genotoxic hepatocarcinogen for the hamster. A study was conducted to follow changes in DNA maintenance methylation in selected genes in liver DNA during the 21-month induction of liver adenomas and hepatocellular carcinomas by demonstrating changes in restriction fragment length polymorphism. Ma...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.12.2711
更新日期:1996-12-01 00:00:00
abstract::The inbred DRH rats are highly resistant to the induction of hepatocellular carcinoma (HCC) by feeding of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB). Previously, we found that two quantitative trait loci (QTLs), Drh1 and Drh2, significantly reduced the number, size and area of glutathione S-transferase-placental ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.1.189
更新日期:2002-01-01 00:00:00
abstract::Male rats were treated with [ring-3H]N-acetyl-2-aminofluorene and sacrificed at different periods of time after a single i.p. dose. Chromatin was isolated from liver homogenates and treated with RNAse and proteinase K. The resulting crude DNA was purified as the N-hexadecyl-N-trimethylammonium salt. The amounts of 2-a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.8.737
更新日期:1981-01-01 00:00:00
abstract::Serum-free cultures of normal human buccal epithelial cells were transfected with a plasmid containing the SV40 T-antigen (SV40T) gene. Two major lines developed that showed extended lifespans (between 30 and 40 weeks) as compared with the controls (approximately 6 weeks). Continued growth through one or two crises ge...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.10.2515
更新日期:1995-10-01 00:00:00
abstract::We have shown previously that the difference between C57BL/6J and C3H/HeJ male mice in their susceptibilities to chemically-induced liver tumors results predominantly from an allelic difference at the Hepatocarcinogen sensitivity (Hcs) locus. This locus modulates the rate of growth of preneoplastic liver lesions and m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.4.589
更新日期:1990-04-01 00:00:00
abstract::Estrogen plays an important role in breast cancer development. While the mechanism of the estrogen effects is not fully elucidated, one possible route is by increasing the stem cell-like properties in the tumors. Tocopherols are known to reduce breast cancer development and progression. The aim of the present study is...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy071
更新日期:2018-07-30 00:00:00
abstract::Untreated and Aroclor 1254-pretreated male Wistar rats were given a single dose of 1.0 mg/kg body weight of randomly tritium-labelled 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (3H-PhIP) by oral intubation. Urine and faeces were collected at 24, 48 and 72 hours after dosing, and total radioactivity determined. A...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.11.2785
更新日期:1995-11-01 00:00:00