Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients.


:DNA double-strand break (DSB) repair is an important mechanism underlying chemotherapy resistance in human cancers. Dicer participates in DSB repair by facilitating homologous recombination. However, whether Dicer is involved in non-homologous end joining (NHEJ) remains unknown. Here, we addressed whether Dicer regulates NHEJ and chemosensitivity in colon cancer cells. Using our recently developed NHEJ assay, we found that DSB introduction by I-SceI cleavage leads to Dicer upregulation. Dicer knockdown increased SIRT7 binding and decreased the level of H3K18Ac (acetylated lysine 18 of histone H3) at DSB sites, thereby repressing the recruitment of NHEJ factors to DSB sites and inhibiting NHEJ. Dicer overexpression reduced SIRT7 binding and increased the level of H3K18Ac at DSB sites, promoting the recruitment of NHEJ factors to DSBs and moderately enhancing NHEJ. Dicer knockdown and overexpression increased and decreased, respectively, the chemosensitivity of colon cancer cells. Dicer protein expression in colon cancer tissues of patients was directly correlated with chemoresistance. Our findings revealed a function of Dicer in NHEJ-mediated DSB repair and the association of Dicer expression with chemoresistance in colon cancer patients.






Chen X,Li WF,Wu X,Zhang HC,Chen L,Zhang PY,Liu LY,Ma D,Chen T,Zhou L,Xu Y,Zhou MT,Tang KF




Has Abstract


2017-09-01 00:00:00














  • Metabolism of benzo[a]pyrene in human epidermal keratinocytes in culture.

    abstract::The metabolism of benzo[a]pyrene (BP) in cultured human epidermal keratinocytes was investigated using thin layer chromatography, high pressure liquid chromatography and cell-mediated mutagenesis assay. Epidermal keratinocytes were obtained from skin of normal subjects and all experiments were performed on primary cul...


    pub_type: 杂志文章


    authors: Kuroki T,Nemoto N,Kitano Y

    更新日期:1980-07-01 00:00:00

  • Differential oxidative stress induced by two different types of skin tumor promoters, benzoyl peroxide and 12-O-tetradecanoylphorbol-13-acetate.

    abstract::The oxidative stress induced in vivo by benzoyl peroxide (BzPo) or 12-O-tetradecanoylphorbol-13-acetate (TPA) was evaluated in terms of chemiluminescence (CL) emitted by SENCAR mouse skin, a non-invasive method that allows an estimation of overall oxidative stress. The ability of a biomimetic superoxide dismutase, cop...


    pub_type: 杂志文章


    authors: Durán HA,de Rey BM

    更新日期:1991-11-01 00:00:00

  • Indirect immunofluorescent localization of benzo[a]pyrene adducted to nucleic acids in cultured mouse keratinocyte nuclei.

    abstract::The localization of benzo[a]pyrene-deoxyguanosine adducts was studied by indirect immunofluorescence in cultured BALB/c epidermal cells exposed to (+/-) 7 alpha, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (the anti-isomer) utilizing an antiserum specific for the major benzo[a]pyrene-de...


    pub_type: 杂志文章


    authors: Poirier MC,Stanley JR,Beckwith JB,Weinstein IB,Yuspa SH

    更新日期:1982-01-01 00:00:00

  • The DNA binding activity of the liver transcription factors LFB1 (HNF1) and HNF4 varies coordinately in rat hepatocellular carcinoma.

    abstract::As renal cell carcinomas are characterized by the disappearance of the transcription factor LFB1, which is known to be primarily involved in gene regulation in the liver, we have measured the presence of LFB1 in rat hepatocellular carcinomas induced by diethylnitrosamine. The level of LFB1 binding activity in adenoid-...


    pub_type: 杂志文章


    authors: Stumpf H,Senkel S,Rabes HM,Ryffel GU

    更新日期:1995-01-01 00:00:00

  • SAA1 is upregulated in gastric cancer-associated fibroblasts possibly by its enhancer activation.

    abstract::Cancer-associated fibroblasts (CAFs) tend to have tumor-promoting capacity, and can provide therapeutic targets. Even without cancer cells, CAF phenotypes are stably maintained, and DNA methylation and H3K27me3 changes have been shown to be involved. Here, we searched for a potential therapeutic target in primary CAFs...


    pub_type: 杂志文章


    authors: Yasukawa Y,Hattori N,Iida N,Takeshima H,Maeda M,Kiyono T,Sekine S,Seto Y,Ushijima T

    更新日期:2020-12-07 00:00:00

  • Novel mechanism of regulation of the DNA repair enzyme OGG1 in tuberin-deficient cells.

    abstract::Tuberin (protein encodes by tuberous sclerosis complex 2, Tsc2) deficiency is associated with the decrease in the DNA repair enzyme 8-oxoG-DNA glycosylase (OGG1) in tumour kidney of tuberous sclerosis complex (TSC) patients. The purpose of this study was to elucidate the mechanisms by which tuberin regulates OGG1. The...


    pub_type: 杂志文章


    authors: Habib SL,Bhandari BK,Sadek N,Abboud-Werner SL,Abboud HE

    更新日期:2010-11-01 00:00:00

  • The effect of the format of administration and the total dose of phenobarbital on altered hepatic foci following initiation in female rats with diethylnitrosamine.

    abstract::The effect of changing the format of administration as well as the total dose of the promoting agent phenobarbital (PB) on the development of altered hepatic foci (AHF) was determined in an initiation-promotion protocol with female rats fed the purified AIN-76 diet. Effects on the total number of AHF and the volume pe...


    pub_type: 杂志文章


    authors: Xu YD,Dragan YP,Young T,Pitot HC

    更新日期:1991-06-01 00:00:00

  • Synergistic effects on the initiation of rat liver tumors by trans-4-acetylaminostilbene and 2-acetylaminofluorene, studied at the level of DNA adduct formation.

    abstract::Both trans-4-acetylaminostilbene (AAS) and 2-acetylaminofluorene (AAF) exert tumor-initiating activity in rat liver when administered in the initiation phase of an initiation-promotion experiment. The effects are more than additive when the compounds are sequentially combined in the initiation phase of such an experim...


    pub_type: 杂志文章


    authors: Ruthsatz M,Neumann HG

    更新日期:1988-02-01 00:00:00

  • Effect of tumour promoters and non-genotoxic carcinogens on terminal differentiation and proliferation in mouse teratoma XB2 cells cultured in low calcium medium.

    abstract::XB2 cells, a teratocarcinoma derived cell line of keratinocyte lineage, have been shown to proliferate and differentiate in low calcium medium (0.03 mM Ca2+) at a density of 500 cells/9.6 cm2 without the need for fibroblast feeder layers or conditioned medium. The degree of differentiation can be assessed by measureme...


    pub_type: 杂志文章


    authors: Hewitt SD,Duffy PA,Orton TC,Garner RC

    更新日期:1990-03-01 00:00:00

  • Oltipraz stimulates the transcription of the manganese superoxide dismutase gene in rat hepatocytes.

    abstract::Oltipraz (4-methyl-5-(2-pyrazinyl)-1,2-dithiole-3-thione) (OPZ) is recognized as a potent chemoprotective agent against chemical-induced carcinogenesis in several animal models and is thought to act mainly by inducing phase II conjugating together with inhibiting phase I detoxication enzymes. The present study was und...


    pub_type: 杂志文章


    authors: Antras-Ferry J,Mahéo K,Chevanne M,Dubos MP,Morel F,Guillouzo A,Cillard P,Cillard J

    更新日期:1997-11-01 00:00:00

  • DNA damage caused by reactive oxygen species originating from a copper-dependent oxidation of the 2-hydroxy catechol of estradiol.

    abstract::It has previously been proposed that redox cycling between catechol estrogens and their quinones, mediated by cytochrome P450, could lead to the generation of free radicals that would subsequently cause oxidative damage to DNA and proteins that might have a role in hormonal carcinogenesis. Alternative, non-enzymatic m...


    pub_type: 杂志文章


    authors: Li Y,Trush MA,Yager JD

    更新日期:1994-07-01 00:00:00

  • Dehydroepiandrosterone and 16 alpha-Br-epiandrosterone inhibit 12-O-tetradecanoylphorbol-13-acetate stimulation of superoxide radical production by human polymorphonuclear leukocytes.

    abstract::Both dehydroepiandrosterone (DHEA) and the synthetic steroid 16 alpha-Br-epiandrosterone (Br-Epi), a more potent inhibitor of glucose-6-phosphate dehydrogenase (G6PDH) than DHEA, inhibit the 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulation of superoxide anion (O2-) formation by human neutrophils. DHEA has previo...


    pub_type: 杂志文章


    authors: Whitcomb JM,Schwartz AG

    更新日期:1985-03-01 00:00:00

  • GSTM1 and CYP2D6 genotype frequencies in patients with pituitary tumours: effects on P53, ras and gsp.

    abstract::We describe studies to determine if susceptibility to pituitary tumours is associated with the putatively high risk GSTM1 null and CYP2D6 EM genotypes. Frequency distributions of these genotypes were similar in cases and controls though the frequency of CYP2D6 PM and GSTM1 B tended to be lower (P = 0.072 and P = 0.095...


    pub_type: 杂志文章


    authors: Perrett CW,Clayton RN,Pistorello M,Boscaro M,Scanarini M,Bates AS,Buckley N,Jones P,Fryer AA,Gilford J

    更新日期:1995-07-01 00:00:00

  • Bacterial formation of N-nitroso compounds from administered precursors in the rat stomach after omeprazole-induced achlorhydria.

    abstract::The role of bacteria in catalysing intragastric formation of N-nitrosothiazolidine-4-carboxylic acid and N-nitrosomorpholine was investigated in a rat model of omeprazole-induced achlorhydria. Omeprazole-treated rats gavaged with nitrosation-proficient bacteria were treated with nitrosamines and/or precursors and comp...


    pub_type: 杂志文章


    authors: Calmels S,Béréziat JC,Ohshima H,Bartsch H

    更新日期:1991-03-01 00:00:00

  • Luteolin, ellagic acid and punicic acid are natural products that inhibit prostate cancer metastasis.

    abstract::Prostate cancer (PCa) is the second cause of cancer deaths in men in the USA. When the cancer recurs, early stages can be controlled with hormone ablation therapy to delay the rate of cancer progression but, over time, the cancer overcomes its hormone dependence, becomes highly aggressive and metastasizes. Clinical tr...


    pub_type: 杂志文章


    authors: Wang L,Li W,Lin M,Garcia M,Mulholland D,Lilly M,Martins-Green M

    更新日期:2014-10-01 00:00:00

  • Modulation of growth and cell turnover of preneoplastic lesions and of prostaglandin levels in rat pancreas by dietary fish oil.

    abstract::In the present study the modulating effects of dietary fish oil (MaxEPA) on unsaturated fat-promoted pancreatic carcinogenesis in azaserine-treated rats were investigated. Three groups of 20 rats (each group comprised five saline-treated and 15 azaserine-treated animals) were fed an AIN76-based purified diet containin...


    pub_type: 杂志文章


    authors: Appel MJ,Woutersen RA

    更新日期:1994-10-01 00:00:00

  • Changes in response to, and production of, transforming growth factor type beta during neoplastic progression in cultured rat tracheal epithelial cells.

    abstract::As rat tracheal epithelial cells progress from a normal to a neoplastic phenotype there are systematic changes in their ability to produce and activate latent transforming growth factor type beta (TGF-beta) as well as systematic changes in their response to this growth factor. Using a TGF-beta radioreceptor binding co...


    pub_type: 杂志文章


    authors: Terzaghi-Howe M

    更新日期:1989-06-01 00:00:00

  • Metabolism of benzo[a]pyrene in primary cultures of human hepatocytes: dose-response over a four-log range.

    abstract::Cultures of isolated human hepatocytes from three different human liver specimens were exposed for 24 h to media containing [3H]benzo[a]pyrene (BP) (0.1, 1.0, 10, 100 microM). The cells and media were harvested and extracted. Subsequent incubations of the aqueous phase with beta-glucuronidase and aryl sulfatase, follo...


    pub_type: 杂志文章


    authors: Monteith DK,Novotny A,Michalopoulos G,Strom SC

    更新日期:1987-07-01 00:00:00

  • Carcinogenic polycyclic aromatic hydrocarbons increase intracellular Ca2+ and cell proliferation in primary human mammary epithelial cells.

    abstract::Previous studies have shown that polycyclic aromatic hydrocarbons (PAHs) mobilize intracellular Ca2+ in human T cells by inositol trisphosphate-dependent mechanisms resulting from activation of phospholipase C-gamma by SRC-related protein tyrosine kinases, thereby mimicking antigen-receptor activation. Ca2+ appears to...


    pub_type: 杂志文章


    authors: Tannheimer SL,Barton SL,Ethier SP,Burchiel SW

    更新日期:1997-06-01 00:00:00

  • Inactivating mutations of the human base excision repair gene NEIL1 in gastric cancer.

    abstract::Oxidized DNA base lesions, such as thymine glycol (Tg) and 8-hydroxyguanine, are often toxic and mutagenic and have been implicated in carcinogenesis. To clarify whether NEIL1 protein, which exhibits excision repair activity towards such base lesions, is involved in gastric carcinogenesis, we examined 71 primary gastr...


    pub_type: 杂志文章


    authors: Shinmura K,Tao H,Goto M,Igarashi H,Taniguchi T,Maekawa M,Takezaki T,Sugimura H

    更新日期:2004-12-01 00:00:00

  • Pre-diagnostic leukocyte mitochondrial DNA copy number and skin cancer risk.

    abstract::No previous study has examined the association between mitochondrial DNA copy number (mtCN) and skin cancer risk prospectively. We examined the associations between peripheral blood leukocytes mtCN level and the risks of skin cancers in a case-control study nested within the Nurses' Health Study of non-Hispanic White ...


    pub_type: 杂志文章


    authors: Meng S,De Vivo I,Liang L,Giovannucci E,Tang JY,Han J

    更新日期:2016-09-01 00:00:00

  • A critical role of non-classical MHC in tumor immune evasion in the amphibian Xenopus model.

    abstract::Non-classical class Ib (class Ib) genes are found in all jawed vertebrates, including the amphibian Xenopus, which possesses at least 20 distinct Xenopus non-classical class Ib genes (XNCs). As an immune evasion strategy, tumors often downregulate surface expression of classical major histocompatibility complex class ...


    pub_type: 杂志文章


    authors: Haynes-Gilmore N,Banach M,Edholm ES,Lord E,Robert J

    更新日期:2014-08-01 00:00:00

  • Association between expression of transforming growth factor-alpha and progression of hepatocellular foci to neoplasms.

    abstract::Hepatocarcinogenesis was initiated in rats with a single dose of either of two chemical mutagens--benzo[a]pyrene diolepoxide I and methyl(acetoxymethyl)nitrosamine--administered 15 h after partial hepatectomy. The development of hepatocellular foci and neoplasms was then promoted with dietary phenobarbital given for 4...


    pub_type: 杂志文章


    authors: Kaufmann WK,Zhang Y,Kaufman DG

    更新日期:1992-08-01 00:00:00

  • Effects of nickel compounds on incorporation of [3H] thymidine into DNA in rat liver and kidney.

    abstract::In vivo incorporation of [3H] thymidine into DNA was determined in rats at 28 h after partial hepatectomy. Administration of nickel carbonyl (Ni(CO)4) at 2 or 4 h before sacrifice inhibited [3H] thymidine uptake into liver and kidney DNA. For example, in rats killed 4 h after i.v. injection of Ni(CO)4 (2 mg Ni/100 g),...


    pub_type: 杂志文章


    authors: Hui G,Sunderman FW Jr

    更新日期:1980-04-01 00:00:00

  • YAP is a candidate oncogene for esophageal squamous cell carcinoma.

    abstract::Yes-associated protein (YAP), the nuclear effector of the Hippo pathway, is a key regulator of organ size and a candidate human oncogene located at chromosome 11q22. Since we previously reported amplification of 11q22 region in esophageal squamous cell carcinoma (ESCC), in this study we focused on the clinical signifi...


    pub_type: 杂志文章


    authors: Muramatsu T,Imoto I,Matsui T,Kozaki K,Haruki S,Sudol M,Shimada Y,Tsuda H,Kawano T,Inazawa J

    更新日期:2011-03-01 00:00:00

  • Characterization of drug-metabolizing systems in hyperplastic nodules from the livers of rats receiving 2-acetylaminofluorene in their diet.

    abstract::A number of drug-metabolizing systems were measured in hyperplastic noduli from the livers of rats receiving 2-acetyl-aminofluorene in their diet and compared with corresponding activities in control liver. The level of microsomal cytochrome P-450 is reduced 54% in the nodular tissue, while 5 activities catalyzed by t...


    pub_type: 杂志文章


    authors: Aström A,DePierre JW,Eriksson L

    更新日期:1983-01-01 00:00:00

  • Occupational exposure to heavy metals: DNA damage induction and DNA repair inhibition prove co-exposures to cadmium, cobalt and lead as more dangerous than hitherto expected.

    abstract::Co-exposure to cadmium, cobalt, lead and other heavy metals occurs in many occupational settings, such as pigment and batteries production, galvanization and recycling of electric tools. However, little is known about interactions between several heavy metals. In the present study we determined DNA single strand break...


    pub_type: 杂志文章


    authors: Hengstler JG,Bolm-Audorff U,Faldum A,Janssen K,Reifenrath M,Götte W,Jung D,Mayer-Popken O,Fuchs J,Gebhard S,Bienfait HG,Schlink K,Dietrich C,Faust D,Epe B,Oesch F

    更新日期:2003-01-01 00:00:00

  • Association between genetic polymorphisms of DNA base excision repair genes and evolution of precancerous gastric lesions in a Chinese population.

    abstract::Base excision repair pathway may play an important role in repairing DNA damage related to Helicobacter pylori-induced inflammatory process. To evaluate the association between genetic polymorphisms of X-ray repair cross-complementing group 1 (XRCC1, Arg194Trp and Arg399Gln), adenosine diphosphate ribosyl transferase ...


    pub_type: 杂志文章


    authors: Li WQ,Zhang L,Ma JL,Zhang Y,Li JY,Pan KF,You WC

    更新日期:2009-03-01 00:00:00

  • Polyphenol-rich sweet potato greens extract inhibits proliferation and induces apoptosis in prostate cancer cells in vitro and in vivo.

    abstract::Sweet potato (Ipomoea batatas) leaves or greens, extensively consumed as a vegetable in Africa and Asia, are an excellent source of dietary polyphenols such as anthocyanins and phenolic acids. Here, we show that sweet potato greens extract (SPGE) has the maximum polyphenol content compared with several commercial vege...


    pub_type: 杂志文章


    authors: Karna P,Gundala SR,Gupta MV,Shamsi SA,Pace RD,Yates C,Narayan S,Aneja R

    更新日期:2011-12-01 00:00:00

  • Sodium arsenite administration via drinking water increases genome-wide and Ha-ras DNA hypomethylation in methyl-deficient C57BL/6J mice.

    abstract::Arsenic is an established human carcinogen. Deficiencies in available animal models have inhibited a detailed analysis of the mechanism of arsenic induced cancer. This study sought to determine the role of a methyl-deficient diet in combination with sodium arsenite on the genomic methylation status and Ha-ras methylat...


    pub_type: 杂志文章


    authors: Okoji RS,Yu RC,Maronpot RR,Froines JR

    更新日期:2002-05-01 00:00:00