Abstract：
:Oxygenated derivatives of cholesterol (oxysterols), a family of naturally occurring compounds, possess marked anti-proliferative and immunosuppressive activities, in particular they have been shown to inhibit T-cell responses to different stimuli. 25-Hydroxycholesterol (25-OHC) and 7 beta,25-dihydroxycholesterol (7.25-OHC) are able to kill not only RDM4 murine lymphoma in vitro, but also, surprisingly, mouse thymocytes after several hours of incubation. In this study, we report that the death of RDM4 and thymocytes induced by oxysterols exhibits the features of apoptosis. This phenomenon was identified by agarose gel electrophoresis of DNA fragments extracted from the cells and quantified by flow cytometric analysis of the DNA fluorescence of propidium iodide-stained cells. Cycloheximide and actinomycin D were found to decrease the number of apoptotic cells and to increase cell viability, indicating a requirement for the synthesis of macromolecules in oxysterol-induced programmed cell death. The pathway by which 25-OHC and 7.25-OHC are able to induce apoptosis in this type of cell and the possible contribution of these compounds to thymus involution during development are discussed.
journal_name
Immunologyjournal_title
Immunologyauthors
Christ M,Luu B,Mejia JE,Moosbrugger I,Bischoff Psubject
Has Abstractpub_date
1993-03-01 00:00:00pages
455-60issue
3eissn
0019-2805issn
1365-2567journal_volume
78pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Dendritic cells (DC) generated from MUTZ-3, an immortalized acute myeloid leukaemia-derived cell line, have potential application as a model for the study of human DC, and as a tool with which to stimulate immunotherapeutic responses to cancer. However, the relationship of MUTZ-3 DC to their non-transformed counterpar...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2008.03018.x
更新日期：2009-07-01 00:00:00
abstract：:Fifteen sera of patients with visceral leishmaniasis were investigated for the occurrence of autoantibodies. They were found in high incidence and titre, and with specificity to the intermediate filament (INFIL) proteins vimentin (12 out of 15 with a titre higher than 1:10) and keratin (9 out of 15 with a titre higher...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Isolated normal human peripheral neutrophils became cytotoxic to chicken red blood cells (CRBC) in the presence of phytohaemagglutinin (PHA) and concanavalin A (Con A), a phenomenon which we have termed lectin-dependent neutrophilmediated cytotoxicity (LDNMC). Substantial cytotoxicity could be demonstrated by 1 h of i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:Feeding antigens induces an immunological unresponsiveness termed oral tolerance but under some conditions, for example following the administration of cyclophosphamide (CY), immunity can be induced. These observations have usually been made by studying antibody production and delayed hypersensitivity with little atte...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease in which abnormal immune responses are mediated by tissue-binding autoantibodies and immune complex deposition. Because most SLE patients are women of child-bearing age, oestrogen has been suggested to play an important role in SLE pathogenesis. One proposed ...
journal_title：Immunology
pub_type： 临床试验,杂志文章
doi：10.1111/imm.12263
更新日期：2014-08-01 00:00:00
abstract：:Tumour necrosis factor-alpha (TNF-alpha) stimulated invasion by mouse T-cell hybridomas and cytotoxic T-lymphocyte clones into rat embryo fibroblast monolayers. The effect on these highly invasive cells was limited: invasion was stimulated maximally to 130% of controls. However, when cells were pretreated with pertuss...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:While many aspects of memory T-cell immunobiology have been characterized, we suggest that we know only a fraction of the effector functions that CD4 T cells can bring to bear during secondary challenges. Exploring the full impact of memory CD4 T-cell responses is key to the development of improved vaccines against ma...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2010.03259.x
更新日期：2010-05-01 00:00:00
abstract：:Detection of immune complexes by inhibition of antibody-dependent cellular cytotoxicity (ADCC) is based on the principle that soluble complexes can compete with target cell-bound antibody for receptors (FcR) on cytotoxic lymphocytes. The objective of this study was to define a cytotoxicity system for the determination...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:Antiserum specific for hamster thymus-derived lymphocytes, prepared by immunization of rabbits with brain tissue from MHA/ssLAK hamsters, was, in the presence of guinea-pig complement, cytotoxic to hamster thymocytes greater than lymph node cells greater than spleen cells, while virtually unreactive against bone marro...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:The microfibrillar protein (MFP) of foetal bovine ligamentum nuchae elastic fibres was prepared and used to produce a monospecific antiserum. Indirect immunofluorescence studies employing the specific antiserum demonstrated its selectivity for MFP associated with elastic fibres. The antiserum was demonstrated to react...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Natural killer (NK) cells have a specialized function in peripheral organs, which is determined by the organ-specific niches. We have attempted to explore whether lung NK cells display a particular phenotype according to their function in the unique pulmonary environment in health or during respiratory infection in mi...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2012.03607.x
更新日期：2012-09-01 00:00:00
abstract：:Airway mucus hyperproduction is a common feature of chronic airway diseases such as severe asthma, chronic obstructive pulmonary disease and cystic fibrosis, which are closely associated with neutrophilic airway inflammation. S100A8, S100A9 and S100A12 are highly abundant proteins released by neutrophils and have been...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12352
更新日期：2015-01-01 00:00:00
abstract：:CD4(+) FOXP3(+) regulatory T (Treg) cells constitute a heterogeneous and plastic T-cell lineage that plays a pivotal role in maintaining immune homeostasis and immune tolerance. However, the fate of human Treg cells after loss of FOXP3 expression and the epigenetic mechanisms contributing to such a phenotype switch re...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12198
更新日期：2014-03-01 00:00:00
abstract：:Pre-exposure to bacterial lipopolysaccharide (LPS) or certain cytokines is known to enhance the ability of murine macrophages to generate a respiratory burst in response to subsequent stimulation, a phenomenon referred to as 'priming'. We report here that the cytokine interleukin-4 (IL-4) can prime murine macrophages....
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Immunoglobulin E (IgE)-dependent mechanisms play a pivotal role in mediating allergic disease. Previously, VH-Cepsilon transcripts from blood or spleen of atopic asthmatics have been analysed for VH gene usage and patterns of somatic mutation. An over-representation of the minor VH5 family has been observed, consisten...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00910.x
更新日期：1999-12-01 00:00:00
abstract：:We have analysed the heavy and light chain variable region genes of two monoclonal antibodies, specific for the Sm antigen (RSP1; IgG kappa) and for cardiolipin (RSP4; IgM lambda), derived from a patient with active systemic lupus erythematosus (SLE). We have established that the variable region genes of the RSP1 auto...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Immunodeficiency syndromes associated with protein-energy malnutrition (PEM) have been documented extensively, although to date the mechanism underlying these defects remains uncharacterized. In this study, we have evaluated T, B, and antigen-presenting cell functions of malnourished mice fed a 4% protein diet compare...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:In the study described here, the effect of human tears and purified lactoferrin was investigated on the deposition of complement components on solid-phase bound immune complexes. After incubating immune complexes with fresh normal human serum, the deposition of complement components (C3, C4 and C5) was measured with a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:The phenotypes of lymphocytes in the large intestinal patches (LIP) of lambs were examined by flow cytometry and immunohistology, using a panel of monoclonal antibodies (mAb), and compared to those found in the jejunal (JPP) and ileal Peyer's patches (IPP). T-cell markers were detected on 25% of the LIP and JPP lympho...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:Human leukaemia and lymphoma cell lines were investigated as stimulating cells in an allogeneic mixed lymphocyte response. Purified T cells and unfractionated mononuclear cells from normal donors were used as responders. The cell lines fell into three groups: (i) those which stimulated allogeneic responder T cells in ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
abstract：:Human haematopoietic progenitor/stem cells (HPCs) differentiate into functional T cells in the thymus through a series of checkpoints. A convenient in vitro system will greatly facilitate the understanding of T-cell development and future engineering of therapeutic T cells. In this report, we established a lentiviral ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2008.03013.x
更新日期：2009-09-01 00:00:00
abstract：:We quantified immunoglobulin E (IgE) on peritoneal mast cells of interleukin-4 (IL-4)-gene knockout (-/-) mice and wild-type (+/+) controls using a cytofluorometric method, and examined the expression of IgE receptors, estimated by quantifying the total binding of IgE on the mast cells of IL-4 (-/-) mice. The mast cel...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00727.x
更新日期：1999-04-01 00:00:00
abstract：:Apoptosis of macrophages infected with pathogenic mycobacteria is an alternative host defence capable of removing the environment supporting bacterial growth. In this work the influence of virulence and bacterial load on apoptosis of alveolar macrophages during the initial phase of infection by Mycobacterium bovis was...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2009.03062.x
更新日期：2009-09-01 00:00:00
abstract：:The vitamin D receptor (VDR) is a nuclear receptor expressed in a number of different cells of the immune system. This study was performed to determine the effect of VDR deficiency on immune function and inflammation of the gastrointestinal tract in a model of inflammatory bowel disease, namely interleukin-10 (IL-10) ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02290.x
更新日期：2006-03-01 00:00:00
abstract：:A murine anti-B monoclonal antibody was obtained by the hybridoma technique. This antibody called anti-B (b-183) is of IgM nature; it is capable of agglutinating normal B, B3, Bx, cis AB and some acquired B red cells. Its association constant is 1.1 X 10(8) l/mol, and appears high compared to those of the monoclonal a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:The interaction of mouse intestinal mast cell proteinase (IMCP) with serine proteinase inhibitors (serpins) in blood was analysed: (i) by examining the capacity of the inhibitors in blood to block the binding of the irreversible serine esterase inhibitor [3H]diisopropyl fluorophosphate (DFP); (ii) by Western blotting....
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:T lymphocytes are a critical component of the adaptive immune system mediating protection against infection and malignancy, but also implicated in many immune pathologies. Upon recognition of specific antigens T cells clonally expand, traffic to inflamed sites and acquire effector functions, such as the capacity to ki...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12655
更新日期：2017-01-01 00:00:00
abstract：:Murine spleen cells were analysed for their capacity to lyse 51Cr-labelled antibody-sensitized erythrocytes and a human lymphoma cell line. Incubation of spleen cells with iron powder followed by removal of iron-containing cells with a magnet significantly decreased the lytic capacity of the remaining cells against er...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The opossum major histocompatibility complex (MHC) shares a similar organization with that of non-mammals while containing a diverse set of class I genes more like that of eutherian (placental) mammals. There are 11 class I loci in the opossum MHC region, seven of which are known to be transcribed. The previously desc...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2008.02994.x
更新日期：2009-09-01 00:00:00
abstract：:The effect of disodium cromoglycate (DSCG) upon human immunoglobulin (Ig) isotypes and IgG subclasses production by purified B cells was studied. DSCG enhanced IgM, IgG1, IgG2, IgG3, IgG4 and IgA production in a dose-dependent fashion, while DSCG failed to induce IgE production at any concentrations tested by purified...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
