Abstract:
:Thiol compounds have been reported to abolish hypoxanthine/xanthine oxidase induced luminol chemiluminescence and this effect has been attributed to scavenging of superoxide (O2-)/(H2O2) produced from hypoxanthine/xanthine oxidase. Yet other workers have reported that thiol compounds have shown little, if any, reactivity towards O2-/H2O2. The aim of this study was to examine the discrepancy between these two sets of findings further. Captopril (a thiol angiotensin-converting enzyme (ACE) inhibitor) and MPG (a simple thiol) were observed to abolish hypoxanthine/xanthine oxidase induced chemiluminescence. The reactivity of captopril and MPG towards O2-/H2O2 was then determined by measurement of thiol oxidation in captopril and MPG after their incubation with hypoxanthine/xanthine oxidase. Incubation (at 10 min, 37 degrees C) with 4 mM hypoxanthine/0.03 u ml-1 xanthine oxidase resulted in 7% and 20% thiol oxidation in captopril and MPG (at 1 mM) respectively. Captopril and MPG, therefore, appeared to be ineffective scavengers of oxidants produced by hypoxanthine/xanthine oxidase. Captopril and MPG also did not affect urate production or oxygen consumption by xanthine oxidase which indicated that captopril and MPG quench luminol chemiluminescence by a mechanism that excludes the inhibition of xanthine oxidase. Hypoxanthine/xanthine oxidase induced luminol chemiluminescence may, therefore, be an unsuitable method for measuring free radical scavenging activity by drugs.
journal_name
Br J Clin Pharmacoljournal_title
British journal of clinical pharmacologyauthors
Clapperton M,McMurray J,Fisher AC,Dargie HJdoi
10.1111/j.1365-2125.1995.tb05730.xsubject
Has Abstractpub_date
1995-06-01 00:00:00pages
688-91issue
6eissn
0306-5251issn
1365-2125journal_volume
39pub_type
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journal_title:British journal of clinical pharmacology
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doi:10.1111/j.1365-2125.1983.tb02179.x
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journal_title:British journal of clinical pharmacology
pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章,随机对照试验
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1983-07-01 00:00:00
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更新日期:2011-02-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2019-10-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2015-10-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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更新日期:2006-04-01 00:00:00