Abstract：
:Regional specific antibodies and gel permeation chromatography were used to analyze the molecular forms of motilin in the human gut and plasma and in the porcine gut. The same antibodies were also used for immunocytochemical localization of the cell of origin of motilin. Two main molecular forms of motilin were identified, peak I eluting at a volume suggesting a larger molecular weight substance (Kav = 0.16) and peak II coeluting with the porcine motilin standard (Kav = 0.67). Peak I accounted for over 50% of the total motilin immunoreactivity in the human gut, whereas in the porcine intestine it represented less than 5%. Peak I thus appeared to have a greater N-terminal sequence similarity with the porcine motilin standard. Chromatographic analysis of human plasma showed the presence of both immunoreactive peaks in approximately equal amounts which were both suppressed following infusion of somatostatin. Infusion of porcine motilin resulted in the expected rise of plasma immunoreactivity in the peak II position, whereas peak I was significantly depressed, suggesting the possibility of a negative feedback. Immunocytochemistry revealed that both antibodies immunostained equally the non argentaffin cell, which contained small round, electron-dense granules. In addition some argentaffin enterochromaffin cells were stained predominantly with the N-terminal specific antibody.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Christofides ND,Bryant MG,Ghatei MA,Kishimoto S,Buchan AM,Polak JM,Bloom SRsubject
Has Abstractpub_date
1981-02-01 00:00:00pages
292-300issue
2eissn
0016-5085issn
1528-0012pii
S0016508581000413journal_volume
80pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract：:Approximately 5% of the world's human population have an increased risk for developing liver cancer and cirrhosis as a direct consequence of chronic infection with the hepatitis B virus (HBV). Antiviral chemotherapy remains the only option for controlling infection in these individuals, for whom the current licensed h...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1016/s0016-5085(00)70008-4
更新日期：2000-02-01 00:00:00
abstract：BACKGROUND/AIMS:Liver fat-storing cells (FSCs) actively proliferate and secrete extracellular matrix during liver injury. Platelet-derived growth factor (PDGF) is a potent mitogen for cultured FSCs. In the present study, we investigated the regulation of PDGF gene expression and production in cultured human liver FSCs....
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(94)90236-4
更新日期：1994-10-01 00:00:00
abstract：:The symptoms of urgency, diarrhea, and abdominal discomfort are exaggerated after a meal in inflammatory disorders of the colon, such as ulcerative colitis. The possible motor correlates of these symptoms were investigated in a model of acute colitis in six dogs. Each dog was instrumented with seven strain gauge trans...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(91)90389-3
更新日期：1991-12-01 00:00:00
abstract：BACKGROUND & AIMS:Previous studies indicated that the peptide neurotensin (NT) stimulates Cl(-) secretion in animal small intestinal mucosa in vitro. In this study, we investigated whether NT causes Cl(-) secretion in human colonic mucosa and examined the mechanism of this response. METHODS:Human mucosal preparations ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2000.9310
更新日期：2000-08-01 00:00:00
abstract：BACKGROUND AND AIMS:The peptidyl prolyl isomerase Pin1 frequently is overexpressed in hepatocellular carcinoma (HCC). Hepatitis B virus (HBV) is the most common etiologic agent in HCC, and its encoded X-protein (HBx) is oncogenic and possesses a serine-proline motif that may bind Pin1. The role of Pin1 in hepatocarcino...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2006.12.030
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND & AIMS:A Western-style diet, which is high in fat and sugar, can cause significant dyslipidemia and nonalcoholic fatty liver disease; the diet has an especially strong effect in women, regardless of total calorie intake. Dietary supplementation with beneficial microbes might reduce the detrimental effects of...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2020.03.010
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND & AIMS:Polymorphisms in the region of the interleukin (IL)-28B gene on chromosome 19 have been associated with peginterferon-alfa-induced clearance of genotype 1 hepatitis C virus (HCV); there are no data for patients with genotype 2 or 3 HCV. We evaluated the effects of IL-28B polymorphisms on response to t...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1053/j.gastro.2010.05.079
更新日期：2010-09-01 00:00:00
abstract：:Although nocturnal acid secretion has been emphasized in the pathophysiology and treatment of duodenal ulcer, its importance in gastric ulcer disease has been questioned. To explore this area, this multicenter U.S. trial compared the effect of a once-daily nighttime dose of H2-receptor antagonist with placebo on the h...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/0016-5085(89)91489-3
更新日期：1989-10-01 00:00:00
abstract：BACKGROUND & AIMS:Patients with cirrhosis are characterized by a reduced splanchnic vascular resistance and a hyporeactivity to adrenergic vasoconstrictors. So far, their adrenergic splanchnic vascular responsiveness has not been evaluated in vitro. We compared responses to alpha1- and beta2-adrenoceptor stimulation of...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(99)70136-8
更新日期：1999-02-01 00:00:00
abstract：BACKGROUND & AIMS:Intestinal fibrosis and stricture formation are serious complications of Crohn's disease, often requiring surgical intervention. Unfortunately, the mechanisms underlying intestinal fibrosis development are poorly understood, in part because of the lack of relevant animal models. Here, we present a nov...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2007.12.043
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND & AIMS:Patients with irritable bowel syndrome with diarrhea (IBS-D) have increased mucosal serotonin (5-hydroxytryptamine [5-HT]) availability, possibly because immune activation reduces activity of the 5-HT transporter (SERT). We investigated the relationship between mucosal and platelet SERT and immune act...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2011.01.052
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND & AIMS:Barrier functions across epithelia and endothelia are essential for homeostatic tissue regulation. Astroglia interact with cerebral endothelia to maintain the blood-brain barrier. Whether similar interactions between astrocyte-like enteric glia and epithelia regulate intestinal barrier function is not...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2007.01.051
更新日期：2007-04-01 00:00:00
abstract：:The factors responsible for prevention of duodenal mucosal injury are not known. This series of experiments was performed to determine whether the human duodenum secretes bicarbonate that could prevent mucosal damage. To isolate a 4-cm segment of proximal (i.e., the duodenal bulb) or distal duodenum free of contaminat...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:Gastrinomas were localized by concurrent blood sampling in the hepatic vein and portal vein tributaries in 10 of 12 Zollinger-Ellison patients. In one patient, the portal vein could not be catheterized; in the other, a gastrin gradient could not be picked up in either of two examinations. Six of the patients were subs...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:A patient with a lifelong history of asthma and hay fever was investigated because of symptoms of esophageal spasm. Esophageal biopsies revealed elongated papillae and basal zone hyperplasia of the epithelial layer with eosinophilic infiltration of the lamina propria and muscularis mucosae. There was no evidence of re...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：BACKGROUND & AIMS:The function of microRNA (miRNA) in liver development is unknown. To address this issue, we characterized miRNA expression in the embryonic mouse liver, performed functional miRNA analysis in zebrafish larvae, and identified novel hepatic miRNA targets. METHODS:Hepatic RNA isolated from mice at embry...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2008.12.006
更新日期：2009-03-01 00:00:00
abstract：:The role of leukotrienes in the pathogenesis of chronic colitis was investigated using a rat model. Ulceration and inflammation of the distal colon was initiated by the intracolonic administration of the hapten trinitrobenzene sulfonic acid in 50% ethanol. Leukotriene B4 synthesis increased significantly within 4 h af...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(89)90760-9
更新日期：1989-01-01 00:00:00
abstract：BACKGROUND & AIMS:The early detection of colorectal cancer is desired because this cancer can be cured surgically if diagnosed early. The purpose of the present study was to determine the feasibility of a new methodology for isolating colonocytes from naturally evacuated feces, followed by cytology or molecular biology...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2005.10.007
更新日期：2005-12-01 00:00:00
abstract：:Using an in vivo canine chambered stomach preparation, the clearance of [14C]aminopyrine across mucosa when intravenously infused and the back-diffusion of this substance from gastric lumen to mucosa when topically applied to gastric epithelium were evaluated in aspirin-damaged gastric epithelium. In mucosa damaged by...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
abstract：BACKGROUND & AIMS:Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. METHODS:Adult rats on ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2002.35353
更新日期：2002-09-01 00:00:00
abstract：BACKGROUND/AIMS:Recent in vivo studies using proton magnetic resonance (1H-MR) spectroscopy showed low levels of myo-inositol in the brain in hepatic encephalopathy; the pathogenetic relevance of this observation is unclear. METHODS:Myo-inositol and glutamine levels in the brain were studied in vivo by 1H-MR spectrosc...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(94)90552-5
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND & AIMS:In patients with achalasia esophagus, swallows induce simultaneous pressure waves known as esophageal pressurization. We studied the mechanism of esophageal pressurization and bolus flow patterns in patients with type 2 or type 3 achalasia. METHODS:We recorded high-resolution manometry with impedance...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2018.04.033
更新日期：2018-08-01 00:00:00
abstract：:Congenital esophageal stenosis (CES) is a rare disorder with narrowed esophageal lumen that presents as dysphagia from childhood and that is often associated with tracheobronchial remnants or webs. The pathogenesis of CES is unknown. The aim of this study was to examine the histological and immunohistochemical feature...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(95)90294-5
更新日期：1995-07-01 00:00:00
abstract：:Previously, we reported that two glycoproteins, carcinoembryonic antigen and secretory component, are preferentially expressed on the apical or laterobasal surfaces, respectively, of normal human colonic epithelial cells, whereas these antigens may be expressed over the entire cell surface of colonic cancer cells. The...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(88)90420-9
更新日期：1988-02-01 00:00:00
abstract：BACKGROUND & AIMS:Studies on the early steps in the life cycle of hepatitis B virus have been hampered by the lack of readily available target cells. In this study, we mapped a defined virus attachment site to primary hepatocytes that is essential for infection. METHODS:We used purified virus particles from human carr...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2005.03.090
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND & AIMS:Therapies for perianal fistulas in patients with Crohn's disease are often ineffective in producing long-term healing. We performed a randomized placebo-controlled trial to determine the long-term efficacy and safety of a single local administration of allogeneic expanded adipose-derived stem cells (C...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1053/j.gastro.2017.12.020
更新日期：2018-04-01 00:00:00
abstract：:Plasma amino acid abnormalities are common in alcoholics, but the respective role of alcoholism, nutrition, and liver injury in producing these abnormalities is unknown. To elucidate this question, amino acids were measured in 56 alcoholics and 32 nonalcoholics with liver disease, and in an experimental model of alcoh...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:The association of hepatobiliary disorders with colonic inflammation is well recognized. Although the pathophysiology is obscure, increased permeation of toxic bacterial products across the inflamed gut to the portal circulation might be one mechanism. Potentially toxic metabolites include N-formylated chemotactic pep...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(88)90560-4
更新日期：1988-04-01 00:00:00
abstract：:Interstitial pneumonitis is an uncommon complication of low-dose methotrexate therapy in patients with psoriasis but occurs in 3%-5% of patients with rheumatoid arthritis. We found a higher incidence of interstitial pneumonitis in patients with primary biliary cirrhosis (14%) and describe its clinical manifestations, ...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章
doi：10.1016/0016-5085(94)90085-x
更新日期：1994-07-01 00:00:00
abstract：:Chloroquine and metronidazole were compared in a randomized trial for the treatment of amoebic liver abscess in 36 patients. An additional 30 patients were treated without randomization. Chloroquine was given according to the standard protocol at Los tangeles County-University of Southern California Medical Center, wh...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1975-07-01 00:00:00
