Lipid metabolism of isolated mouse pancreatic islets maintained in culture at different glucose concentrations.

Abstract:

:The lipid metabolism of isolated mouse pancreatic islets, which had been cultured for one week at 3.3 or 16.7 mM glucose, was examined in short-term experiments performed at the end of the culture period. It was found that: a) high-glucose cultured islets oxidized palmitate at a higher rate: and b) both groups of cultured islets increased the rate of incorporation of (U-14C)-glucose into triacylglycerols and phospholipids in response to an acute glucose challenge. This latter effect was, however, more pronounced after culture at a high glucose concentration. The present data suggest that long-term exposure to high concentrations of glucose leads to a general increase of islet oxidative metabolism. Moreover, high glucose culture seems to induce an increased conversion of carbohydrates into triacylglycerols and membrane phospholipids.

journal_name

Ups J Med Sci

authors

Berne C,Andersson A

doi

10.3109/03009738109179210

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

55-61

issue

1

eissn

0300-9734

issn

2000-1967

journal_volume

86

pub_type

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