Structure of tubulin rings studied by X-ray scattering using synchrotron radiation.

abstract：:Bovine leukemia virus (BLV) is a deltaretrovirus that infects domestic cattle. The structural protein Gag, found in all retroviruses, is a polyprotein comprising three major functional domains: matrix (MA), capsid (CA), and nucleocapsid (NC). Previous studies have shown that both mature BLV MA and NC are able to bind ...

journal_title：Journal of molecular biology

authors： Qualley DF,Cooper SE,Ross JL,Olson ED,Cantara WA,Musier-Forsyth K

更新日期：2019-03-15 00:00:00

abstract：:Membrane crystals of mitochondrial ubiquinol: cytochrome c reductase of improved size and long-range order and of the cytochrome bc1 subcomplex have been obtained by a dialysis method. The enzyme--Triton X-100 complex was mixed with Triton phospholipid micelles and the Triton slowly removed by dialysis for 48 hours at...

journal_title：Journal of molecular biology

authors： Hovmöller S,Slaughter M,Berriman J,Karlsson B,Weiss H,Leonard K

更新日期：1983-04-05 00:00:00

abstract：:The periplasmic domain of the aspartate chemotaxis receptor from Salmonella typhimurium has been crystallized in the presence and absence of bound aspartate. Both crystal forms were grown by precipitation with lithium sulfate and diffract to 1.8 A resolution. The aspartate receptor structure is believed to be prototyp...

journal_title：Journal of molecular biology

authors： Jancarik J,Scott WG,Milligan DL,Koshland DE Jr,Kim SH

更新日期：1991-09-05 00:00:00

abstract：:Contact-dependent growth inhibition (CDI) is a widespread mechanism of inter-bacterial competition mediated by the CdiB/CdiA family of two-partner secretion proteins. CdiA effectors carry diverse C-terminal toxin domains (CdiA-CT), which are delivered into neighboring target cells to inhibit growth. CDI(+) bacteria al...

journal_title：Journal of molecular biology

authors： Morse RP,Willett JL,Johnson PM,Zheng J,Credali A,Iniguez A,Nowick JS,Hayes CS,Goulding CW

更新日期：2015-11-20 00:00:00

abstract：:Due to regulatory feedback, biological networks can exist stably in multiple states, leading to heterogeneous phenotypes among genetically identical cells. Random fluctuations in protein numbers, tuned by specific molecular mechanisms, have been hypothesized to drive transitions between these different states. We deve...

journal_title：Journal of molecular biology

authors： Choi PJ,Xie XS,Shakhnovich EI

更新日期：2010-02-12 00:00:00

abstract：:We used site-specific recombination catalyzed by the bacteriophage lambda Int system to probe DNA structure and metabolism in vivo. In vitro, the complexity of catenated products was linearly proportional to substrate supercoil density. A system was developed that gave efficient, controlled Int recombination in Escher...

journal_title：Journal of molecular biology

authors： Bliska JB,Cozzarelli NR

更新日期：1987-03-20 00:00:00

abstract：:A phage-neutralizing rabbit antiserum collected after immunization with tail-fiberless bacteriophage T4 particles was adsorbed with complete T4 phage. The resulting adsorbed serum inhibited tail fiber attachment in vitro. To identify the antigens against which this inhibitory activity was directed, blocking experiment...

journal_title：Journal of molecular biology

authors： Urig MA,Brown SM,Tedesco P,Wood WB

更新日期：1983-09-15 00:00:00

abstract：:Transposable-element-mediated fusion of the conjugal plasmid pOX38::Tn9 with pBR322 results in the appearance of cointegrates composed of a single copy of each plasmid, and cointegrates which carry a single copy of pOX38 but multiple tandem copies of pBR322. These plasmids are separated by directly repeated copies of ...

journal_title：Journal of molecular biology

authors： Chandler M,Galas DJ

更新日期：1983-03-25 00:00:00

abstract：:Mitochondrial function depends on the correct synthesis, transport, and assembly of proteins and cofactors of the electron transport chain. The initial idea that the respiratory chain protein complexes (RCCs) were independent structures in the inner mitochondrial membrane evolved after the identification of higher qua...

journal_title：Journal of molecular biology

authors： Cogliati S,Lorenzi I,Rigoni G,Caicci F,Soriano ME

更新日期：2018-12-07 00:00:00

abstract：:The Mycobacterium leprae RuvA homologue (MlRuvA) was over-expressed in Escherichia coli and purified to homogeneity. The DNA-binding specificity and the functional interactions of MlRuvA with E. coli RuvB and RuvC (EcRuvB and EcRuvC) were examined using synthetic Holliday junctions. MlRuvA bound specifically to Hollid...

journal_title：Journal of molecular biology

authors： Arenas-Licea J,van Gool AJ,Keeley AJ,Davies A,West SC,Tsaneva IR

更新日期：2000-08-25 00:00:00

abstract：:Group II intron-encoded proteins (IEPs), which have maturase and reverse transcriptase activities, form a ribonucleoprotein (RNP) complex with the intron RNA. Some IEPs also have a C-terminal DNA-binding region and conserved DNA endonuclease domain involved in the recognition and cleavage of specific DNA target sites ...

journal_title：Journal of molecular biology

authors： Jiménez-Zurdo JI,García-Rodríguez FM,Barrientos-Durán A,Toro N

更新日期：2003-02-14 00:00:00

abstract：:When theoretical methods are used to predict the properties of a given system, such as the effects of the substitution of a specific amino acid on the activity or stability of a protein as a whole, the accuracy of the prediction is directly dependent on the validity of the underlying model. A common error, however, is...

journal_title：Journal of molecular biology

authors： van Gunsteren WF,Mark AE

更新日期：1992-09-20 00:00:00

abstract：:FtsZ is part of a mid-cell cytokinetic structure termed the Z-ring that recruits a hierarchy of fission related proteins early in the bacterial cell cycle. The widely conserved ZapA has been shown to interact with FtsZ, to drive its polymerisation and to promote FtsZ filament bundling thereby contributing to the spati...

journal_title：Journal of molecular biology

authors： Low HH,Moncrieffe MC,Löwe J

更新日期：2004-08-13 00:00:00

abstract：:A mechanism proposed for lactose/H(+) symport by the lactose permease of Escherichia coli indicates that lactose permease is protonated prior to ligand binding. Moreover, in the ground state, the symported H(+) is shared between His322 (helix X) and Glu269 (helix VIII), while Glu325 (helix X) is charge-paired with Arg...

journal_title：Journal of molecular biology

authors： Weinglass AB,Sondej M,Kaback HR

更新日期：2002-01-25 00:00:00

abstract：:Six different hybrid colicins were constructed by recombining various domains of the two pore-forming colicins A and E1. These hybrid colicins were purified and their properties were studied. All of them were active against sensitive cells, although to varying degrees. From the results, one can conclude that: (1) the ...

journal_title：Journal of molecular biology

authors： Benedetti H,Frenette M,Baty D,Knibiehler M,Pattus F,Lazdunski C

更新日期：1991-02-05 00:00:00

abstract：:We have determined the three-dimensional organization of ribosomal RNAs and proteins essential for minimal ribosome function. Comparative sequence analysis identifies regions of the ribosome that have been evolutionarily conserved, and the spatial organization of conserved domains is determined by mapping these onto s...

journal_title：Journal of molecular biology

authors： Mears JA,Cannone JJ,Stagg SM,Gutell RR,Agrawal RK,Harvey SC

更新日期：2002-08-09 00:00:00

abstract：:Two types of annelid collagens of different sizes were purified, one from acetic acid extracts of the cuticle (length 2.5 microns) and the other, after pepsin digestion, from interstitial spaces of the body wall (0.3 micron). They were obtained from Alvinella pompejana, Alvinella caudata and Paralvinella grasslei coll...

journal_title：Journal of molecular biology

authors： Gaill F,Mann K,Wiedemann H,Engel J,Timpl R

更新日期：1995-02-17 00:00:00

abstract：:The histone-like nucleoid structuring (H-NS) protein is a global modulator of gene expression in Gram-negative bacteria. VicH, the H-NS protein of Vibrio cholerae, regulates the expression of certain major virulence determinants implicated in the pathogenesis of cholera. We present here the 2.5A crystal structure of t...

journal_title：Journal of molecular biology

authors： Cerdan R,Bloch V,Yang Y,Bertin P,Dumas C,Rimsky S,Kochoyan M,Arold ST

更新日期：2003-11-21 00:00:00

abstract：:Dengue virus (DV) infection depends on a step of membrane fusion, which occurs in the acidic environment of the endosome. This process is mediated by virus surface envelope glycoprotein, in which the loop between residues D98-G112 is considered to be crucial, acting as a fusion peptide. Here, we have characterized fun...

journal_title：Journal of molecular biology

authors： Melo MN,Sousa FJ,Carneiro FA,Castanho MA,Valente AP,Almeida FC,Da Poian AT,Mohana-Borges R

更新日期：2009-09-25 00:00:00

abstract：:The human kinetochore is a highly complex macromolecular structure that connects chromosomes to spindle microtubules (MTs) in order to facilitate accurate chromosome segregation. Centromere-associated protein E (CENP-E), a member of the kinesin superfamily, is an essential component of the kinetochore, since it is req...

journal_title：Journal of molecular biology

authors： Garcia-Saez I,Yen T,Wade RH,Kozielski F

更新日期：2004-07-23 00:00:00

abstract：:Carbon catabolite repression (CCR) of several operons in Bacillus subtilis and Bacillus megaterium is mediated by the cis-acting cre sequence and trans-acting catabolite control protein (CcpA). We describe purification of CcpA from B. megaterium and its interaction with regulatory sequences from the xyl operon. Specif...

journal_title：Journal of molecular biology

authors： Gösseringer R,Küster E,Galinier A,Deutscher J,Hillen W

更新日期：1997-03-07 00:00:00

abstract：:The metazoan cell membrane is highly organized. Maintaining such organization and preserving membrane integrity under different conditions are accomplished through intracellular tethering to an extensive, flexible protein network. Spectrin, the principal component of this network, is attached to the membrane through t...

journal_title：Journal of molecular biology

authors： Yasunaga M,Ipsaro JJ,Mondragón A

更新日期：2012-04-06 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) genome encodes a highly conserved regulatory gene product, Vpr (96 residues, 14kDa), which is incorporated into virions. In the infected cells, Vpr, expressed late in the virus cycle, is believed to function in the early phases of HIV-1 replication, such as nuclear migra...

journal_title：Journal of molecular biology

authors： Morellet N,Bouaziz S,Petitjean P,Roques BP

更新日期：2003-03-14 00:00:00

abstract：:Complex and diverse signal transduction circuits are responsible for the efficient functioning of cellular network. Protein kinases and O-protein phosphatases are primarily responsible for propagating such stimuli within a eukaryotic cell. However, there is limited understanding of O-protein phosphatases in the prokar...

journal_title：Journal of molecular biology

authors： Bhaduri A,Sowdhamini R

更新日期：2005-09-23 00:00:00

abstract：:The ribosomal protein L30 from yeast Saccharomyces cerevisiae auto-regulates its own synthesis by binding to a structural element in both its pre-mRNA and its mRNA. The three-dimensional structures of L30 in the free (f L30) and the pre-mRNA bound (b L30) forms have been solved by nuclear magnetic resonance spectrosco...

journal_title：Journal of molecular biology

authors： Mao H,Williamson JR

更新日期：1999-09-17 00:00:00

abstract：:Rat intestinal fatty-acid-binding protein (I-FABP) is a small (15,124 Mr) cytoplasmic polypeptide that binds long-chain fatty acids in a non-covalent fashion. I-FABP is a member of a family of intracellular binding proteins that are thought to participate in the uptake, transport and/or metabolic targeting of hydropho...

journal_title：Journal of molecular biology

authors： Sacchettini JC,Gordon JI,Banaszak LJ

更新日期：1989-07-20 00:00:00

abstract：:Recently, the absence of a thermally induced transition has been offered as proof that the unfolded and the molten globule states of apo-alpha-lactalbumin are enthalpically equivalent. In this paper we demonstrate that that argument is thermodynamically incorrect. In addition, it is shown that the absence of a thermal...

journal_title：Journal of molecular biology

authors： Xie D,Bhakuni V,Freire E

更新日期：1993-07-05 00:00:00

abstract：:Ebolaviruses are causative agents of lethal hemorrhagic fever in humans and nonhuman primates. Among the filoviruses characterized thus far, Reston Ebola virus (REBOV) is the only Ebola virus that is nonpathogenic to humans despite the fact that REBOV can cause lethal disease in nonhuman primates. Previous studies als...

journal_title：Journal of molecular biology

authors： Leung DW,Shabman RS,Farahbakhsh M,Prins KC,Borek DM,Wang T,Mühlberger E,Basler CF,Amarasinghe GK

更新日期：2010-06-11 00:00:00

abstract：:The genes coding for translation initiation factor IF3 (infC) and for the ribosomal proteins L35 (rpmI) and L20 (rplT) are transcribed in that order from a promoter in front of infC. The last two cistrons of the operon (rpmI and rplT) can be transcribed from a weak secondary promoter situated within the first cistron ...

journal_title：Journal of molecular biology

authors： Lesage P,Truong HN,Graffe M,Dondon J,Springer M

更新日期：1990-06-05 00:00:00

abstract：:SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive complex 1, a large multiprotein assembly required for the repression of developmental control genes. It contains two MBT (malignant brain tumor) repeats; the MBT is a protein module structurally similar to domains that bind to methylated his...

journal_title：Journal of molecular biology

authors： Santiveri CM,Lechtenberg BC,Allen MD,Sathyamurthy A,Jaulent AM,Freund SM,Bycroft M

更新日期：2008-10-24 00:00:00