A double helix B-type geometry based on high-resolution proton NMR of single-helical DNA fragments: d(TA)5 x d(TA)5.

abstract：:A dnaB-like protein from P. aeruginosa was purified to near homogeneity using as an assay the immunoprecipitation by E. coli dnaB antiserum in a solid-phase. In the chromatographic characteristics including the affinity to immobilized ATP the dnaB-like protein of P. aeruginosa is similar to the dnaB protein of E. coli...

journal_title：Nucleic acids research

authors： Dreiseikelmann B,Riedel HD,Schuster H

更新日期：1987-01-26 00:00:00

abstract：:A molecular rotor thioflavin T (ThT) is usually used as a fluorescent ligand specific for G-quadruplexes. Here, we demonstrate that ThT can tightly bind non-G-quadruplex DNAs with several GA motifs and dimerize them in a parallel double-stranded mode, accompanied by over 100-fold enhancement in the fluorescence emissi...

journal_title：Nucleic acids research

authors： Liu S,Peng P,Wang H,Shi L,Li T

更新日期：2017-12-01 00:00:00

abstract：:We have examined the behaviour of the beta-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the beta-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the beta-interferon p...

journal_title：Nucleic acids research

authors： Ellis MJ,Goodbourn S

更新日期：1994-10-25 00:00:00

abstract：:In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...

journal_title：Nucleic acids research

authors： Faust M,Hastings KE,Millward S

更新日期：1975-08-01 00:00:00

abstract：:Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-le...

journal_title：Nucleic acids research

authors： Ilyin Y,Chmeliauskaite VG,Kulguskin VV,Georgiev GP

更新日期：1980-11-25 00:00:00

abstract：:Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor expressed in metazoan cells that is responsible for eliciting the production of type I interferons and pro-inflammatory cytokines upon detection of intracellular, non-self RNA. Structural studies of RIG-I have identified a novel Pincer domain com...

journal_title：Nucleic acids research

authors： Rawling DC,Kohlway AS,Luo D,Ding SC,Pyle AM

更新日期：2014-10-01 00:00:00

abstract：:The unwinding of supercoiled phi X174 RFI DNA induced by the tumorigenic (+) and non-tumorigenic (-) enantiomers of trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) has been investigated by agarose slab-gel and ethidium titration tube gel electrophoresis. The differences in adduct conformat...

journal_title：Nucleic acids research

authors： Xu R,Birke S,Carberry SE,Geacintov NE,Swenberg CE,Harvey RG

更新日期：1992-12-11 00:00:00

abstract：:The direction of replication of DNA within replicons of Physarum polycephalum was studied by pulse-labelling with 5-bromouracil-deoxyriboside (BrdUrd) and 3H-adenosine deoxyriboside (dAdo), followed by ultraviolet- (UV) -photolysis and analysis of molecular weights of single strand DNA fragments on alkaline sucrose gr...

journal_title：Nucleic acids research

authors： Funderud S,Andreassen R,Haugli F

更新日期：1978-03-01 00:00:00

abstract：:Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support before the 3'-ends are releas...

journal_title：Nucleic acids research

authors： Kwiatkowski M,Fredriksson S,Isaksson A,Nilsson M,Landegren U

更新日期：1999-12-15 00:00:00

abstract：:The binding of two symmetric bis-benzimidazole compounds, 2,2-bis-[4'-(3"-dimethylamino-1"-propyloxy)phenyl]-5,5-bi-1H-benzimidazole and its piperidinpropylphenyl analog, to the minor groove of DNA, have been studied by DNA footprinting, surface plasmon resonance (SPR) methods and molecular dynamics simulations in exp...

journal_title：Nucleic acids research

authors： Bailly C,Chessari G,Carrasco C,Joubert A,Mann J,Wilson WD,Neidle S

更新日期：2003-03-01 00:00:00

abstract：:Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ell...

journal_title：Nucleic acids research

authors： Ikehara M,Fukui T,Kakiuchi N

更新日期：1976-08-01 00:00:00

abstract：:Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...

journal_title：Nucleic acids research

authors： Perales-Clemente E,Fernández-Silva P,Acín-Pérez R,Pérez-Martos A,Enríquez JA

更新日期：2011-01-01 00:00:00

abstract：:Transfection of a human estrogen receptor cDNA expression vector (HEO) into cultured Xenopus kidney cells confers estrogen responsiveness to the recipient cells as demonstrated by the hormone dependent expression of co-transfected Xenopus vitellogenin-CAT chimeric genes. The estrogen stimulation of these vit-CAT genes...

journal_title：Nucleic acids research

authors： Seiler-Tuyns A,Mérillat AM,Haefliger DN,Wahli W

更新日期：1988-09-12 00:00:00

abstract：:The crystal structure of the RNA/DNA hybrid r(GAAGAGAAGC). d(GCTTCTCTTC) has been solved and refined at 2.5 A resolution. The refinement procedure converged at R = 0.181 for all reflections in the range 20.0-2.5 A. In the crystal, the RNA/DNA hybrid duplex has an A' conformation with all but one of the nucleotide suga...

journal_title：Nucleic acids research

authors： Conn GL,Brown T,Leonard GA

更新日期：1999-01-15 00:00:00

abstract：:MicroRNAs (miRNAs) are short, non-protein coding RNAs that direct the widespread phenomenon of post-transcriptional regulation of metazoan genes. The mature approximately 22-nt long RNA molecules are processed from genome-encoded stem-loop structured precursor genes. Hundreds of such genes have been experimentally val...

journal_title：Nucleic acids research

authors： Gerlach D,Kriventseva EV,Rahman N,Vejnar CE,Zdobnov EM

更新日期：2009-01-01 00:00:00

abstract：:The availability of a draft human genome sequence and ability to monitor the transcription of thousands of genes with DNA microarrays has necessitated the need for new computational tools that can analyze cis-regulatory elements controlling genes that display similar expression patterns. We have developed a tool desig...

journal_title：Nucleic acids research

authors： Zhang H,Ramanathan Y,Soteropoulos P,Recce ML,Tolias PP

更新日期：2002-11-01 00:00:00

abstract：:Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...

journal_title：Nucleic acids research

authors： Ray WC,Daniels CJ

更新日期：2003-01-01 00:00:00

abstract：:Sox proteins belong to the HMG box superfamily of DNA-binding proteins and are found throughout the animal kingdom. They are involved in the regulation of such diverse developmental processes as germ layer formation, organ development and cell type specifi-cation. Hence, deletion or mutation of Sox proteins often resu...

journal_title：Nucleic acids research

authors： Wegner M

更新日期：1999-03-15 00:00:00

abstract：:The nucleotide sequence of the 5S ribosomal RNA of Streptococcus cremoris has been determined. The sequence is 5' (sequence in text) 3'. Comparison of the S. cremoris 5S RNA sequence to an updated prokaryotic generalized 5S RNA structural model shows that this 5S RNA contains some unusual structural features. These fe...

journal_title：Nucleic acids research

authors： Neimark H,Andersen J,Delihas N

更新日期：1983-11-11 00:00:00

abstract：:BRENDA (BRaunschweig ENzyme DAtabase) represents a comprehensive collection of enzyme and metabolic information, based on primary literature. The database contains data from at least 83,000 different enzymes from 9800 different organisms, classified in approximately 4200 EC numbers. BRENDA includes biochemical and mol...

journal_title：Nucleic acids research

authors： Schomburg I,Chang A,Ebeling C,Gremse M,Heldt C,Huhn G,Schomburg D

更新日期：2004-01-01 00:00:00

abstract：:Structures of r(CGCGCG)2 and 2'-O-Me(CGCGCG)2 have been determined by NMR spectroscopy under low salt conditions. All protons and phosphorus nuclei resonances have been assigned. Signals of H5'/5" have been assigned stereospecifically. All 3JH,H and 3JP,H coupling constants have been measured. The structures were dete...

journal_title：Nucleic acids research

authors： Popenda M,Biala E,Milecki J,Adamiak RW

更新日期：1997-11-15 00:00:00

abstract：:Intrinsic energetic and solvation factors contributing to the unusual structural and biochemical properties of N3'-phosphoramidate DNA analogs have been re-examined using a combination of quantum mechanical and molecular dynamics methods. Evaluation of the impact of the N3'-H substitution was performed via comparison ...

journal_title：Nucleic acids research

authors： Banavali NK,MacKerell AD

更新日期：2001-08-01 00:00:00

abstract：:A DNA region of 2750 base pairs encompassing the genes III, VI and I of bacteriophage M13 has been sequenced by the Maxam-Gilbert procedure. By establishing the nucleotide changes introduced by several amber mutations, the coding region and the regulatory signals of each gene have been deduced. The genes appear to spa...

journal_title：Nucleic acids research

authors： van Wezenbeek P,Schoenmakers JG

更新日期：1979-06-25 00:00:00

abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...

journal_title：Nucleic acids research

authors： Kong Q,Maizels N

更新日期：2001-03-15 00:00:00

abstract：:The transcripts of a 6Kbp region of the mitochondrial genome of S. cerevisiae, localized in the 21S rRNA-OXI1 span and including 12 tRNA genes (from tRNA(thr) to tRNA(ala)) and several G+C clusters, have been studied by analysis of in vitro capped primary transcripts and by fine mapping of the 5' ends of transcripts. ...

journal_title：Nucleic acids research

authors： Francisci S,Palleschi C,Ragnini A,Frontali L

更新日期：1987-08-25 00:00:00

abstract：:The DNA photosensitisers m-iodo Hoechst and m-iodo, p-methoxy Hoechst have been co-crystallised with the oligonucleotide d(CGCGAATTCGCG)(2)and their crystal structures determined. The crystals were then subjected to slow dehydration, which reduced their solvent contents from 40 (normal) to 30 (partially dehydrated) an...

journal_title：Nucleic acids research

authors： Squire CJ,Baker LJ,Clark GR,Martin RF,White J

更新日期：2000-03-01 00:00:00

abstract：:In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene express...

journal_title：Nucleic acids research

authors： van den Berg A,van der Leij J,Poppema S

更新日期：1999-09-01 00:00:00

abstract：:The Galaxy HiCExplorer provides a web service at https://hicexplorer.usegalaxy.eu. It enables the integrative analysis of chromosome conformation by providing tools and computational resources to pre-process, analyse and visualize Hi-C, Capture Hi-C (cHi-C) and single-cell Hi-C (scHi-C) data. Since the last publicatio...

journal_title：Nucleic acids research

authors： Wolff J,Rabbani L,Gilsbach R,Richard G,Manke T,Backofen R,Grüning BA

更新日期：2020-07-02 00:00:00

abstract：:The Histone Database (HDB) is an annotated and searchable collection of all full-length sequences and structures of histone and non-histone proteins containing the histone fold motif. These sequences are both eukaryotic and archaeal in origin. Several new histone fold-containing proteins have been identified, includin...

journal_title：Nucleic acids research

authors： Sullivan SA,Aravind L,Makalowska I,Baxevanis AD,Landsman D

更新日期：2000-01-01 00:00:00

abstract：:MDg3 is a family of mobile dispersed genetic elements represented by 15 copies in the haploid genome of D. melanogaster and flanked, like other similar elements, by the regions of homology. In the present work, these regions of mdg3 have been sequenced. The existence of perfect direct repeats 268 base pairs long has b...

journal_title：Nucleic acids research

authors： Bayev AA Jr,Krayev AS,Lyubomirskaya NV,Ilyin YV,Skryabin KG,Georgiev GP

更新日期：1980-08-11 00:00:00