Abstract：
:A simple hemolytic plaque assay for the detection of red blood cells carrying abnormal or mutant hemoglobin is described. The assay is based on the use, as indicator cells, of sheep red blood cells coated with monospecific antibody against the hemoglobin variant, another complement fixing multispecific anti-hemoglobin antibody as developing serum and an antibody against red cell ghosts which can lyse only the red cells of the species being studied and not the indicator sheep red cells. The test red cells are mixed with the indicator sheep red cells, the developing antiserum and the anti-ghost antibody. The mixture is transferred into thin chambers prepared with 2 microscope slides and incubated at room temperature (25 degrees C). The indicator sheep red cells around each test red cell carrying abnormal or mutant hemoglobin are lysed resulting in the formation of a plaque. Instead of anti-hemoglobin-coated sheep red blood cells, protein A-coated sheep red blood cells may be used as indicator cells provided the developing antiserum is monospecific for the hemoglobin variant. When sheep red blood cells coated with an antibody specific for DBA/2J mouse hemoglobin were used in the assay, only red cells from DBA/2J mice (carrying d hemoglobin) formed plaques while C57BL/6J red cells (carrying s hemoglobin) did not.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Baig MA,Ansari AA,Malling HVdoi
10.1016/0022-1759(82)90075-8subject
Has Abstractpub_date
1982-11-26 00:00:00pages
43-50issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(82)90075-8journal_volume
55pub_type
杂志文章abstract：:We have previously described a monoclonal antibody (BU-1) to 5-bromo-2-deoxyuridine (BrdUrd) that is useful for measurement of cell cycle S-phase. BU-1 hybridoma supernatant reacted with incorporated BrdUrd after the cells had been ethanol fixed; without a requirement for acid or base denaturation. We have found that ...
journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1986-10-23 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1995-02-27 00:00:00
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journal_title：Journal of immunological methods
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doi：10.1016/s0022-1759(98)00039-8
更新日期：1998-05-01 00:00:00
abstract：:There is a need for simple and sensitive assays to assess innate and adaptive immune responses to microbial agents and vaccines. Herein, we describe a whole blood method allowing to measure the induction of cytokine synthesis at the mRNA level. The originality of this method consists in the combination of PAXgene tube...
journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/s0022-1759(03)00074-7
更新日期：2003-05-01 00:00:00
abstract：:Dendritic cells (DC) are increasingly explored as cellular vaccines for tumor immunotherapy. In most reported DC-based cancer vaccine trials, DC have been pulsed with soluble tumor antigen-derived peptide ligands of MHC molecules. Considering that the half-life of peptide/MHC complexes on the cell surface is relativel...
journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2004.01.010
更新日期：2004-04-01 00:00:00
abstract：:The major histocompatibility complex (MHC) class I related receptor, the neonatal Fc receptor (FcRn), rescues immunoglobulin G (IgG) and albumin from lysosomal degradation by recycling in endothelial cells. FcRn also contributes to passive immunity by mediating transport of IgG from mother to fetus (human) or newborn ...
journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2007.11.003
更新日期：2008-02-29 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(79)90005-x
更新日期：1979-01-01 00:00:00
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journal_title：Journal of immunological methods
pub_type：
doi：10.1016/s0022-1759(01)00358-1
更新日期：2001-07-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(83)90009-1
更新日期：1983-06-24 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(79)90262-x
更新日期：1979-01-01 00:00:00
abstract：:Monoclonal antibodies (Mab) are the fastest growing group of biopharmaceuticals in development. For production in mammalian cells, the four polypeptide chains of the immunoglobulin diheterotetramer must be assembled prior to exit from the endoplasmic reticulum. Various recombinant Mab expression vectors have been deve...
journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2006.10.010
更新日期：2007-01-10 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2008.11.001
更新日期：2009-02-28 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(79)90242-4
更新日期：1979-05-10 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2003.08.014
更新日期：2003-12-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(87)90310-3
更新日期：1987-02-11 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2015.09.003
更新日期：2015-12-01 00:00:00
abstract：INTRODUCTION:Peripheral blood mononuclear cells (PBMCs) are frequently used for genomic analyses, but several factors can affect the yield and integrity of nucleic acids, including the methods of cell collection and isolation. The goal of this work was to analyze the utility of systematic isolation of different immune ...
journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2016.12.005
更新日期：2017-03-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2012.04.003
更新日期：2012-07-31 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：2007-07-31 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2015.10.003
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1989-03-10 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/j.jim.2009.12.003
更新日期：2010-02-28 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/s0022-1759(97)00141-5
更新日期：1997-11-10 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(81)90225-8
更新日期：1981-01-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(90)90337-u
更新日期：1990-02-20 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(90)90280-9
更新日期：1990-12-31 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：2000-11-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/s0022-1759(00)00209-x
更新日期：2000-07-31 00:00:00
abstract：:A two-stage culture method is described for the induction of a specific antibody response to sheep red cells (SRC) in microcultures at limiting dilutions of human peripheral blood lymphocytes (PBL). PBL from normal donors were cultured for 4 days with antigen and EBV using well defined conditions. The cells were then ...
journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(88)90449-8
更新日期：1988-04-22 00:00:00
