Abstract:
:By pre-stimulating the lymphocytes of cancer patients with BCGF plus anti-mu chain specific antibody, we have generated human X human hybridomas. The resulting hybridomas preferentially produced IgG class antibody compared with those obtained from non-stimulated lymphocytes. With non-stimulated lymph-node lymphocytes, from stomach cancer patients we selected a hybrid (TOS/H8) producing IgM with broad reactivity against human tumor cells. In contrast, with pre-stimulated lymph-node lymphocytes from hepatoma patients we selected a hybrid (TOH/D5) producing IgG having a specific reactivity with HuTu80 (duodenum carcinoma).
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Hagiwara H,Nagao Jdoi
10.1016/0022-1759(90)90280-9subject
Has Abstractpub_date
1990-12-31 00:00:00pages
263-71issue
1-2eissn
0022-1759issn
1872-7905pii
0022-1759(90)90280-9journal_volume
135pub_type
杂志文章abstract::Application of antibody phage display to the identification of cell surface antigens with restricted expression patterns is often complicated by the inability to demonstrate specific binding to a certain cell type. The specificity of an antibody can only be properly assessed when the antibody is of sufficient high aff...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
doi:10.1016/0022-1759(91)90120-5
更新日期:1991-06-24 00:00:00
abstract::Three commercial second generation enzyme immunoassays (EIA) and a second generation immunoblot assay (RIBA-II) for detecting antibodies to hepatitis C virus (HCV) were evaluated in a study of confirmatory testing using sera referred by six Regional Blood Transfusion Centres (RTC). Of a total of 490 samples, 203 were ...
journal_title:Journal of immunological methods
pub_type: 杂志文章,多中心研究
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abstract::We present the development and testing of a novel assay of lymphocyte adhesion based on time-resolved morphological measurements of intercellular aggregation. Homotypic lymphocyte aggregation is induced according to various protocols and monitored for several hours using video microscopy and time-lapse recording. Digi...
journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1993-11-05 00:00:00
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pub_type: 杂志文章
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更新日期:1997-03-28 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(79)90005-x
更新日期:1979-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90235-n
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(86)90438-2
更新日期:1986-10-23 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1992-01-21 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(81)90274-x
更新日期:1981-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1976-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/s0022-1759(99)00114-3
更新日期:1999-11-19 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(85)90068-7
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pub_type: 杂志文章
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:2008-01-31 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1983-11-11 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2014-01-15 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
abstract::Surface plasmon resonance is a valuable optical phenomenon for monitoring biomolecular interactions in real time. In this project anti-mouse-Fc was coupled to the carboxymethyl dextran coating on the surface of a CM5 sensor chip (BIAcore) using amine coupling. Monoclonal antibodies (MAbs) to Luteinizing Hormone (LH) w...
journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1998-12-01 00:00:00
abstract::A simple, sensitive and accurate method was developed to determine the level of lymphoid chimerism in bone marrow-transplanted rodents. The method is based on flow cytometry using polyclonal alloantisera and labeled second step anti-IgG antibodies. Using mixtures of spleen cells from different mouse strains, it was de...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90044-v
更新日期:1990-07-03 00:00:00