DNA sequence of the 16S rRNA/23S rRNA intercistronic spacer of two rDNA operons of the archaebacterium Methanococcus vannielii.

abstract：:Recombinase proteins assembled into helical filaments on DNA are believed to be the catalytic core of homologous recombination. The assembly, disassembly and dynamic rearrangements of this structure must drive the DNA strand exchange reactions of homologous recombination. The sensitivity of eukaryotic recombinase acti...

journal_title：Nucleic acids research

authors： Ristic D,Modesti M,van der Heijden T,van Noort J,Dekker C,Kanaar R,Wyman C

更新日期：2005-06-08 00:00:00

abstract：:The Génolevures online database (http://cbi.labri.fr/Genolevures/ and http://genolevures.org/) provides exploratory tools and curated data sets relative to nine complete and seven partial genome sequences determined and manually annotated by the Génolevures Consortium, to facilitate comparative genomic studies of Hemi...

journal_title：Nucleic acids research

authors： Sherman DJ,Martin T,Nikolski M,Cayla C,Souciet JL,Durrens P,Génolevures Consortium.

更新日期：2009-01-01 00:00:00

abstract：:In bacteria, promoter identification by RNA polymerase is mediated by a dissociable σ factor. The housekeeping σ(70) factor of Escherichia coli recognizes two well characterized DNA sequence elements, known as the '-10' and '-35' hexamers. These elements are separated by 'spacer' DNA, the sequence of which is generall...

journal_title：Nucleic acids research

authors： Singh SS,Typas A,Hengge R,Grainger DC

更新日期：2011-07-01 00:00:00

abstract：:The synthesis of cyclic di-, tri-, tetra-, penta- and hexa-thymidylic acids (16; n = 2, 3, 4, 5 and 6) and the cyclic hexadeoxyribonucleotide [d(CpCpTpApGpGp)], via the "filtration" approach, is reported. Some of the physical properties of the cyclic oligonucleotides are discussed, and their susceptibility to digestio...

journal_title：Nucleic acids research

authors： Rao MV,Reese CB

更新日期：1989-10-25 00:00:00

abstract：:Sequences from -3500 to +1365 of the chicken histone H5 gene have been analyzed for the presence of cis-acting elements in H5 expressing (transformed CFU-E) and non-expressing cells (fibroblasts). The region from -3500 to -115 had little effect on transcription. Proximal upstream sequences contain a negative element (...

journal_title：Nucleic acids research

authors： Rousseau S,Renaud J,Ruiz-Carrillo A

更新日期：1989-09-25 00:00:00

abstract：:We recently identified a region of preferential replication initiation, oriGNAI3, near the 3' end of the Chinese hamster GNAI3 gene. oriGNAI3 is co-amplified in mutants selected for AMPD2 amplification, a process generating chromosomal rearrangements. In this report we have taken advantage of cell lines with truncated...

journal_title：Nucleic acids research

authors： Toledo F,Lachagès AM,Mayau V,Debatisse M

更新日期：1999-04-01 00:00:00

abstract：:Human tissue-specific genes were reported to be longer than housekeeping genes (both in coding and intronic parts). The competing neutralist and adaptationist models were proposed to explain this observation. Here I show that in human genome the longest are genes with the intermediate expression pattern. From the stan...

journal_title：Nucleic acids research

authors： Vinogradov AE

更新日期：2006-01-01 00:00:00

abstract：:The emergence of massively parallel sequencing technology has revolutionized microbial profiling, allowing the unprecedented comparison of microbial diversity across time and space in a wide range of host-associated and environmental ecosystems. Although the high-throughput nature of such methods enables the detection...

journal_title：Nucleic acids research

authors： Amir A,Zeisel A,Zuk O,Elgart M,Stern S,Shamir O,Turnbaugh PJ,Soen Y,Shental N

更新日期：2013-12-01 00:00:00

abstract：:The synthesis of ribosomal precursor RNA in Novikoff hepatoma (N1S1) cells is very sensitive to cordycepin (3'-dA). The synthesis of hnRNA, however, is resistant to inhibition concentrations of 3'-dA that completely block the synthesis of 45S ribosomal RNA precursor. We have examined the RNA polymerases present in the...

journal_title：Nucleic acids research

authors： Desrosiers RC,Rottman FM,Boezi JA,Towle HC

更新日期：1976-02-01 00:00:00

abstract：:We report the use of an engineered ribozyme to produce a circular human exon in vitro. Specifically, we have designed a derivative of a yeast self-splicing group II intron that is able to catalyze the formation of a circular exon encoding the first kringle domain (K1) of the human tissue plasminogen activator protein....

journal_title：Nucleic acids research

authors： Mikheeva S,Hakim-Zargar M,Carlson D,Jarrell K

更新日期：1997-12-15 00:00:00

abstract：:We combine comparative genomic measures and the distance separating adjacent genes to predict operons in 124 completely sequenced prokaryotic genomes. Our method automatically tailors itself to each genome using sequence information alone, and thus can be applied to any prokaryote. For Escherichia coli K12 and Bacillu...

journal_title：Nucleic acids research

authors： Price MN,Huang KH,Alm EJ,Arkin AP

更新日期：2005-02-08 00:00:00

abstract：:The gene encoding myelin basic protein (MBP) contains multiple activator sequences spanning upstream of its transcriptional initiation site which differentially promote transcription in glial cells. The proximal activator sequence, designated MB1, activates transcription in a glial cell type specific manner. This sequ...

journal_title：Nucleic acids research

authors： Devine-Beach K,Haas S,Khalili K

更新日期：1992-02-11 00:00:00

abstract：:We studied in vivo transcription and gene expression directed by a series of synthetic sequences, bearing the consensus hexamer (CH) pair of E.coli promoters in various contexts. The results demonstrate that, for the contexts tested, the CH pair supports transcription activity and gene expression, whether the spacer l...

journal_title：Nucleic acids research

authors： Jacquet MA,Reiss C

更新日期：1990-03-11 00:00:00

abstract：:Loop length and its composition are important for the structural and functional versatility of quadruplexes. To date studies on the loops have mainly concerned model sequences compared with naturally occurring quadruplex sequences which have diverse loop lengths and compositions. Herein, we have characterized 36 quadr...

journal_title：Nucleic acids research

authors： Kumar N,Maiti S

更新日期：2008-10-01 00:00:00

abstract：:The 4.5S rRNA was isolated from the chloroplast ribosomes from Dryopteris acuminata. The complete nucleotide sequence was determined to be: OHUAAGGUCACGGCAAGACGAGCCGUUUAUCACCACGAUAGGUGCUAAGUGGAGGUGCAGUAAUGUAUGCAGCUGAGGC AUCCUAAUAGACCGAGAGGUUUGAACOH. The 4.5S rRNA is composed of 103 nucleotides and shows strong homolog...

journal_title：Nucleic acids research

authors： Takaiwa F,Kusuda M,Sugiura M

更新日期：1982-04-10 00:00:00

abstract：:An intronic hexanucleotide UGCAUG has been shown to play a critical role in the regulation of tissue-specific alternative splicing of pre-mRNAs in a wide range of tissues. Vertebrate Fox-1 has been shown to bind to this element, in a highly sequence-specific manner, through its RNA recognition motif (RRM). In mammals,...

journal_title：Nucleic acids research

authors： Nakahata S,Kawamoto S

更新日期：2005-04-11 00:00:00

abstract：:Intragenomic and intergenomic comparisons of upstream nucleotide sequences of archaeal genes were performed with the goal of predicting transcription regulatory sites (operators) and identifying likely regulons. Learning sets for the detection of regulatory sites were constructed using the available experimental data ...

journal_title：Nucleic acids research

authors： Gelfand MS,Koonin EV,Mironov AA

更新日期：2000-02-01 00:00:00

abstract：:Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the template by two tailed long primers and two short primers in a sin...

journal_title：Nucleic acids research

authors： Chiu J,March PE,Lee R,Tillett D

更新日期：2004-12-07 00:00:00

abstract：:The organelle genome database GOBASE, now in its 21st release (June 2008), contains all published mitochondrion-encoded sequences (approximately 913,000) and chloroplast-encoded sequences (approximately 250,000) from a wide range of eukaryotic taxa. For all sequences, information on related genes, exons, introns, gene...

journal_title：Nucleic acids research

authors： O'Brien EA,Zhang Y,Wang E,Marie V,Badejoko W,Lang BF,Burger G

更新日期：2009-01-01 00:00:00

abstract：:The ability to integrate 'omics' (i.e. transcriptomics and proteomics) is becoming increasingly important to the understanding of regulatory mechanisms. There are currently no tools available to identify differentially expressed genes (DEGs) across different 'omics' data types or multi-dimensional data including time ...

journal_title：Nucleic acids research

authors： Tang S,Hemberg M,Cansizoglu E,Belin S,Kosik K,Kreiman G,Steen H,Steen J

更新日期：2016-06-02 00:00:00

abstract：:Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interac...

journal_title：Nucleic acids research

authors： Palm T,Hemmer K,Winter J,Fricke IB,Tarbashevich K,Sadeghi Shakib F,Rudolph IM,Hillje AL,De Luca P,Bahnassawy L,Madel R,Viel T,De Siervi A,Jacobs AH,Diederichs S,Schwamborn JC

更新日期：2013-04-01 00:00:00

abstract：:Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are importan...

journal_title：Nucleic acids research

authors： Haac ME,Anderson MA,Eggleston H,Myles KM,Adelman ZN

更新日期：2015-04-20 00:00:00

abstract：:Cadmium (Cd2+) is a known carcinogen that inactivates the DNA mismatch repair (MMR) pathway. In this study, we have tested the effect of Cd2+ exposure on the enzymatic activity of the mismatch binding complex MSH2-MSH6. Our results indicate that Cd2+ is highly inhibitory to the ATP binding and hydrolysis activities of...

journal_title：Nucleic acids research

authors： Banerjee S,Flores-Rozas H

更新日期：2005-03-03 00:00:00

abstract：:Recently, we described a method for multiplex genome editing by natural transformation (MuGENT). Mutant constructs for MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro DNA splicing. In Vibrio cholerae, we uncover that this requirement is due to cytopl...

journal_title：Nucleic acids research

authors： Dalia TN,Yoon SH,Galli E,Barre FX,Waters CM,Dalia AB

更新日期：2017-07-07 00:00:00

abstract：:With the recent increase in the number of mammalian genomes being sequenced, large-scale genome scans for human-specific positive selection are now possible. Selection can be inferred through phylogenetic analysis by comparing the rates of silent and replacement substitution between related species. Maximum-likelihood...

journal_title：Nucleic acids research

authors： Nickel GC,Tefft D,Adams MD

更新日期：2008-01-01 00:00:00

abstract：:The inducibility of the vir genes (virA, -B, -C, -D, -E, and -G) on pRiA4 was examined at the transcriptional level, and the RNA-starting sites were determined by S1-nuclease mapping and primer-extension experiments. All of these genes were inducible, while virA, -E, and -G were transcribed even under noninducing cond...

journal_title：Nucleic acids research

authors： Aoyama T,Takanami M,Oka A

更新日期：1989-11-11 00:00:00

abstract：:Cell differentiation is a central process in development and in cancer growth and dissemination. OCT4 (POU5F1) and NANOG are essential for cell stemness and pluripotency; yet, the mechanisms that regulate their expression remain largely unknown. Repetitive elements account for almost half of the Human Genome; still, t...

journal_title：Nucleic acids research

authors： Morales-Hernández A,González-Rico FJ,Román AC,Rico-Leo E,Alvarez-Barrientos A,Sánchez L,Macia Á,Heras SR,García-Pérez JL,Merino JM,Fernández-Salguero PM

更新日期：2016-06-02 00:00:00

abstract：:Recent studies indicate that controlling the nuclear decondensation and intra-nuclear localization of plasmid DNA (pDNA) would result in an increased transfection efficiency. In the present study, we established a technology for imaging the nuclear condensation/decondensation status of pDNA in nuclear subdomains using...

journal_title：Nucleic acids research

authors： Shaheen SM,Akita H,Yamashita A,Katoono R,Yui N,Biju V,Ishikawa M,Harashima H

更新日期：2011-04-01 00:00:00

abstract：:The GWIPS-viz browser (http://gwips.ucc.ie/) is an on-line genome browser which is tailored for exploring ribosome profiling (Ribo-seq) data. Since its publication in 2014, GWIPS-viz provides Ribo-seq data for an additional 14 genomes bringing the current total to 23. The integration of new Ribo-seq data has been auto...

journal_title：Nucleic acids research

authors： Michel AM,Kiniry SJ,O'Connor PBF,Mullan JP,Baranov PV

更新日期：2018-01-04 00:00:00

abstract：:Comprehensive understanding of structure and recognition properties of regulatory nucleic acid elements in real time and atomic level is highly important to devise efficient therapeutic strategies. Here, we report the establishment of an innovative biophysical platform using a dual-app nucleoside analog, which serves ...

journal_title：Nucleic acids research

authors： Nuthanakanti A,Ahmed I,Khatik SY,Saikrishnan K,Srivatsan SG

更新日期：2019-07-09 00:00:00