Abstract：
:The growth factors FGF and vasopressin were found to have only a transient effect on confluent quiescent monolayers of Swiss 3T3 cells. Whether measured as cumulative entry into S-phase by autoradiography, or as cell division by time-lapse filming, the elevated rate of cell proliferation was maintained only over 10-15 hr. Several trivial or artifactual explanations for this transience were ruled out, including toxicity of 3H-thymidine; exhaustion or degradation of medium components, nutrients or growth factors (although some medium depletion was observed); and the generation during quiescence of cells incapable of division. We have also eliminated heritable variation in the capacity to respond to individual growth factors. However, unstable phenotypic heterogeneity in growth factor requirements between cells may play some part, as found elsewhere for the response to low concentrations of serum (Brooks et al, 1984). Cell populations that had ceased to respond to vasopressin recovered their sensitivity after 2-3 days' incubation in conditioned medium lacking vasopressin. The phenomenon thus resembles the mitogen-induced desensitization described by Collins and Rosengurt (1982, 1983). However, in our case, the loss of sensitivity was not selective for vasopressin but applied also to epidermal growth factor (EGF) and to prostaglandin F2 alpha. Furthermore, changes in responsiveness to vasopressin with time were associated with changes in cell density. Although some element of selective desensitization has not been ruled out, the transient response in our experiments can be accounted for in terms of unstable heterogeneity in growth factor requirements and/or in terms of density-dependent regulation of growth.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Richmond KM,Riddle PN,Brooks RFdoi
10.1002/jcp.1041210314subject
Has Abstractpub_date
1984-12-01 00:00:00pages
547-57issue
3eissn
0021-9541issn
1097-4652journal_volume
121pub_type
杂志文章abstract：:The hypothesis of functional hemizygosity has been examined for the alpha-amanitin resistant (AmaR, a codominant marker) locus in a series of Chinese hamster cell lines. AmaR mutants were obtained from different cell lines, e.g., CHO, DHW, M3- 1 and CHO-Kl, at similar frequencies. After fractionation of different RNA ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040970321
更新日期：1978-12-01 00:00:00
abstract：:Epithelial cells of the airways can, under pathological conditions, undergo squamous metaplasia. The accumulation of cholesterol sulfate has recently been described as a new marker for squamous cell differentiation in rabbit tracheal epithelial cells. We now report that normal human bronchial epithelial cells in cultu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041330320
更新日期：1987-12-01 00:00:00
abstract：:Isolated hamster intestinal epithelial cells can be separated by velocity sedimentationion on 2-10% Ficoll gradients into three subpopulations of cells which differ in morphology, biochemistry, physiology, and membrane components. These subpopulations are not pure but are enriched in a single cell type to the extent t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041110111
更新日期：1982-04-01 00:00:00
abstract：:Resistance to conventional chemotherapy remains a major cause of cancer relapse and cancer-related deaths. Therefore, there is an urgent need to overcome resistance barriers. To improve cancer treatment approaches, it is critical to elucidate the basic mechanisms underlying drug resistance. Increasingly, the mechanism...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.29640
更新日期：2020-10-01 00:00:00
abstract：:Receptors for urokinase (uPA) and plasminogen provide a mechanism to direct the cellular activation of plasminogen. The regulation of these receptors is important for several macrophage functions. In these studies, the effect of transforming growth factor-beta 1 (TGF-beta 1) on uPA, uPA receptor, and plasminogen recep...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1041640214
更新日期：1995-08-01 00:00:00
abstract：:Eukaryotic organisms package DNA into chromatin for compact storage in the cell nucleus. However, this process promotes transcriptional repression of genes. To overcome the transcriptional repression, chromatin remodeling complexes have evolved that alter the configuration of chromatin packaging of DNA into nucleosome...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.22533
更新日期：2011-08-01 00:00:00
abstract：:Proteasome inhibitors represent a promising therapy for the treatment of relapsed and/or refractory multiple myeloma, a disease that is concomitant with osteolysis and enhanced osteoclast formation. While blockade of the proteosome pathway has been recently shown to influence osteoclast formation and function, the pre...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.21787
更新日期：2009-08-01 00:00:00
abstract：:Both the Na+-dependent glucose cotransporter (SGLT1) and the cystic fibrosis transmembrane conductance regulator (CFTR) modulate Na+ and fluid movement, although in opposite directions. Yet few studies have investigated a possible interrelationship between these two transporters. By using the Caco-2 human colon carcin...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199809)176:3<472::AID-JCP4
更新日期：1998-09-01 00:00:00
abstract：:The transient nuclear localization of metallothionein during cell growth and differentiation may be related to the increased requirement of zinc for DNA synthesis, activation of metalloenzymes, and transcription factors. Treatment of 3T3-L1 fibroblasts with aphidicolin, an inhibitor of nuclear DNA synthesis, caused a ...
journal_title：Journal of cellular physiology
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doi：10.1002/(SICI)1097-4652(200005)183:2<247::AID-JCP1
更新日期：2000-05-01 00:00:00
abstract：:The vagina provides a characteristic low-Na+ and low-pH fluid microenvironment that is considered generally protective. Previous studies have shown that various types of epithelial cells harbor the capacity of intracellular pH (pHi) regulation. However, it remains elusive whether vaginal epithelium could actively regu...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.28237
更新日期：2019-01-29 00:00:00
abstract：:The endogenous thiols (PSH, protein sulfhydryls; NPSH, nonprotein sulfhydryls; and GSH, glutathione) were measured in the 66 and 67 murine carcinoma cells growing under different physiological conditions in vitro (e.g., proliferation, P; nutrient-deprived quiescence QI; and QI cells stimulated by refeeding the monolay...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041320121
更新日期：1987-07-01 00:00:00
abstract：:An isoform of the Na(+)/Ca(2+) exchanger (SDNCX1.10) was cloned from mesangial cells of Sprague-Dawley rat. Regulation of this isoform was compared to two other clones that were derived from the Dahl/Rapp salt sensitive (SNCX) and salt resistant rat (RNCX). All isoforms differ at the alternative splice site and at ami...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10447
更新日期：2004-05-01 00:00:00
abstract：:This study aimed at investigating the expression and function of uracil nucleotide-sensitive receptors (P2Y(2), P2Y(4), and P2Y(6)) on osteogenic differentiation of human bone marrow stromal cells (BMSCs) in culture. Bone marrow specimens were obtained from postmenopausal female patients (68 ± 5 years old, n = 18) und...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.23014
更新日期：2012-06-01 00:00:00
abstract：:Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were incubated with diffe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25249
更新日期：2016-07-01 00:00:00
abstract：:Endothelin (ET)-1 was originally characterized as a potent vasoconstrictor peptide secreted by vascular endothelial cells. It possesses a wide range of biological activities within the cardiovascular system and in other organs, including the brain. Also secreted by endothelial cells, nitric oxide (NO), has recently be...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041550114
更新日期：1993-04-01 00:00:00
abstract：:The increased levels of c-Myc protein observed previously in an ovarian carcinoma cell line stably transfected to express HER2 has suggested a role for the HER2 pathway in c-Myc expression. Analysis of HER2-transfected cells stimulated with heregulin beta1 (HRG) revealed increased c-Myc protein levels but not a corres...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.20012
更新日期：2004-07-01 00:00:00
abstract：:Tumor necrosis factor-α (TNF-α) is a pluripotent signaling molecule. The biological effect of TNF-α includes slowing down osteogenic differentiation, which can lead to bone dysplasia in long-term inflammatory microenvironments. Signal transducer and activator of transcription 3 (STAT3)-interacting protein 1 (StIP1, al...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.28440
更新日期：2019-08-01 00:00:00
abstract：:Cells of confluent cultures of the established pig renal epithelial line, LLC-PK1, accumulate alpha-methyl-D-glucoside against a concentration gradient. This transport system is strongly inhibited by phlorizin and 6-deoxy-D-glucose, moderately inhibited by phloretin, and only weakly inhibited by 3-0-methyl-D-glucose, ...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1041040311
更新日期：1980-09-01 00:00:00
abstract：:Selenium has been associated with cancer prevention. Despite vast knowledge of selenium effect on various health conditions, functional characterization of selenium metabolic enzymes on cellular physiology has been limited. Therefore, to gain insight into the mechanisms underlying cancer prevention by selenium, we inv...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.20714
更新日期：2006-10-01 00:00:00
abstract：:Autophagy is an evolutionarily conserved intracellular process and is considered one of the main catabolism pathways. In the process of autophagy, cells are digested nonselectively or selectively to recover nutrients and energy, so it is regarded as an antiaging process. In addition to the essential role of autophagy ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2021-01-16 00:00:00
abstract：:Breast cancer is a common cancer leading to many deaths among females. Cyclooxygenase-2 (COX-2) and interleukin-8 (IL-8) are two highly expressed inflammatory mediators to be induced by the protein kinase C (PKC) signaling via various inflammatory stimuli and both contribute significantly to cancer metastasis/progress...
journal_title：Journal of cellular physiology
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doi：10.1002/jcp.24955
更新日期：2015-09-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/(SICI)1097-4652(200003)182:3<429::AID-JCP1
更新日期：2000-03-01 00:00:00
abstract：:The untransformed stable cell line Met murine hepatocytes (MMH), generated from liver explants of transgenic mice expressing a constitutively active truncated form of the human hepatocyte growth factor receptor (cyto-Met), represents an innovative tool for in vitro studies of liver function. In the present report, we ...
journal_title：Journal of cellular physiology
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doi：10.1002/(SICI)1097-4652(199910)181:1<24::AID-JCP3>
更新日期：1999-10-01 00:00:00
abstract：:Tumor cells secrete extracellular vesicles (EVs) for intercellular communication. EVs by transporting different proteins, nucleic acids, and lipids contribute to affect target cell function and fate. EVs which originate directly from multivesicular bodies so-called exosomes have dramatically fascinated the attention ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
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更新日期：2020-10-01 00:00:00
abstract：:In order to study the generation, factors controlling endogenous folate pools, and their functional importance, Friend erythroleukemia cells were grown in media containing 100; 1,000; and 10,000 ng/ml of tritiated pteroylglutamic acid (3H)PteGlu1 and then studied in unlabeled media with varying amounts of PteGlu1. The...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041140216
更新日期：1983-02-01 00:00:00
abstract：:Lung diseases are major cause of morbidity and mortality worldwide. The progress in regenerative medicine and stem cell research in the lung are currently a fast-growing research topic that can provide solutions to these major health problems. Under normal conditions, the rate of cellular proliferation is relatively l...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.26414
更新日期：2018-10-01 00:00:00
abstract：:Low-power laser irradiation (LPLI) has been shown to exert promotive effects on cell survival and proliferation through activation of various signaling pathways. Estrogen receptors (ERs, ERα, and ERβ) are ligand-activated transcription factors, which regulate target gene expression, promote cell proliferation, and res...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24252
更新日期：2013-05-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF), a potent mediator of endothelial proliferation and migration, has an important role also in brain edema formation during hypoxia and ischemia. VEGF binds to the tyrosine kinase receptors Flt-1 and Flk-1. Yet, their relative importance for hypoxia-induced hyperpermeability is ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21022
更新日期：2007-07-01 00:00:00
abstract：:The predominant cyclooxygenase products of keratinocytes are prostaglandin (PG)E2 and PGF2 alpha with only trace amounts of PGI2 synthesis detected. When normal or immortal (NM1) keratinocytes were co-cultured with mitomycin C-treated 3T3 cells, increased synthesis of PGI2 was noted compared to mitomycin C-treated 3T3...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041500208
更新日期：1992-02-01 00:00:00
abstract：:Experiments were carried out to test the hypothesis that cell spreading and phagocytosis are similar cell responses to different-sized substrata. The following morphological and biochemical studies provided evidence for this supposition. Cells phagocytosed 1.09-micron and 5.7-micron latex beads, but were unable to com...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041190110
更新日期：1984-04-01 00:00:00
