Developing dorsal root ganglion neurons require trophic support from their central processes: evidence for a role of retrogradely transported nerve growth factor from the central nervous system to the periphery.

abstract：:This work argues that, in a polarized environment, scientists can minimize the likelihood that the audience's biased processing will lead to rejection of their message if they not only eschew advocacy but also, convey that they are sharers of knowledge faithful to science's way of knowing and respectful of the audienc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamieson KH,Hardy BW

更新日期：2014-09-16 00:00:00

abstract：:We have examined the kinetics of the interaction between endodeoxyribonuclease EcoRI (EC 3.1.23.13) and nine linear DNA fragments that range in size between 34 and 6,200 base pairs and contain the EcoRI site of plasmid pBR322 in a central location. The kinetic parameters governing both formation and decay of specific ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jack WE,Terry BJ,Modrich P

更新日期：1982-07-01 00:00:00

abstract：:A transcription factor required for synthesis of accurately initiated run-off transcripts by RNA polymerase II has been purified and shown to have an associated DNA-dependent ATPase (dATPase) activity that is strongly stimulated by the TATA region of promoters. This transcription factor, designated delta, was purified...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conaway RC,Conaway JW

更新日期：1989-10-01 00:00:00

abstract：:P/Q Ca(2+) channel activity is inhibited by G protein-coupled receptor activation. Channel inhibition requires a direct Gbetagamma binding onto the pore-forming subunit, Ca(v)2.1. It is characterized by biophysical changes, including current amplitude reduction, activation kinetic slowing, and an I-V curve shift, whic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandoz G,Lopez-Gonzalez I,Grunwald D,Bichet D,Altafaj X,Weiss N,Ronjat M,Dupuis A,De Waard M

更新日期：2004-04-20 00:00:00

abstract：:Sequencing of a 360-nucleotide segment of the mitochondrial control region for 63 individuals from an Amerindian tribe, the Nuu-Chah-Nulth of the Pacific Northwest, revealed the existence of 28 lineages defined by 26 variable positions. This represents a substantial level of mitochondrial diversity for a small local p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ward RH,Frazier BL,Dew-Jager K,Pääbo S

更新日期：1991-10-01 00:00:00

abstract：:Tumor-infiltrating lymphocytes (TIL) obtained from human melanomas can specifically lyse autologous tumor in vitro and mediate tumor regression in vivo. To develop more effective therapeutic reagents and to further understand the T-cell response to tumors, the diversity of T-cell receptors (TCRs) involved in melanoma ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shilyansky J,Nishimura MI,Yannelli JR,Kawakami Y,Jacknin LS,Charmley P,Rosenberg SA

更新日期：1994-03-29 00:00:00

abstract：:The role of microtubules in 3H-labeled protein secretion in rat lacrimal glands was probed by the use of colchicine and two of its analogues that reversibly bind to tubulin. These analogues were 2-methoxy-5-(2,3,4,4'-trimethoxyphenyl)-2,4,6-cycloheptatriene-1-o ne and 2,3,4,4'-tetramethoxy-1,1'-biphenyl, the latter ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herman G,Busson S,Gorbunoff MJ,Mauduit P,Timasheff SN,Rossignol B

更新日期：1989-06-01 00:00:00

abstract：:The binding of interferons to distinct cell surface receptors leads to the induction of synthesis of several unique polypeptides and their corresponding mRNAs (1-6). We have isolated two cDNAs that are complementary to nuclear RNA whose synthesis is induced from undetectable levels to maximal rates of transcription wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Larner AC,Jonak G,Cheng YS,Korant B,Knight E,Darnell JE Jr

更新日期：1984-11-01 00:00:00

abstract：:Spectrin is an important structural component of the plasma membrane skeleton. Heretofore-unidentified isoforms of spectrin also associate with Golgi and other organelles. We have discovered another member of the beta-spectrin gene family by homology searches of the GenBank databases and by 5' rapid amplification of c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stankewich MC,Tse WT,Peters LL,Ch'ng Y,John KM,Stabach PR,Devarajan P,Morrow JS,Lux SE

更新日期：1998-11-24 00:00:00

abstract：:The binding of chylomicron remnants rat liver plasma membranes was studied. Liver membranes bound up to 8 times more remnants than they bound chylomicrons. The remnant particle appeared to bind to the membrane as a unit. Remnant binding was greatest to liver plasma membrane; only one third as much binding was observed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrella M,Cooper AD

更新日期：1979-01-01 00:00:00

abstract：:Terrorist attacks often fuel online hate and increase the expression of xenophobic and antiminority messages. Previous research has focused on the impact of terrorist attacks on prejudiced attitudes toward groups linked to the perpetrators as the cause of this increase. We argue that social norms can contain the expre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Álvarez-Benjumea A,Winter F

更新日期：2020-09-15 00:00:00

abstract：:Transcription factors (TFs) are primary regulators of gene expression in cells, where they bind specific genomic target sites to control transcription. Quantitative measurements of TF-DNA binding energies can improve the accuracy of predictions of TF occupancy and downstream gene expression in vivo and shed light on h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le DD,Shimko TC,Aditham AK,Keys AM,Longwell SA,Orenstein Y,Fordyce PM

更新日期：2018-04-17 00:00:00

abstract：:By considering temperature effects on the mechanical unfolding rates of proteins and RNA, whose energy landscape is rugged, the question posed in the title is answered in the affirmative. Adopting a theory by Zwanzig [Zwanzig, R. (1988) Proc. Natl. Acad. Sci. USA 85, 2029-2030], we show that, because of roughness char...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyeon C,Thirumalai D

更新日期：2003-09-02 00:00:00

abstract：:The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgiev PG,Corces VG

更新日期：1995-05-23 00:00:00

abstract：:8-Oxoguanine (8OG) is efficiently bypassed by RNA polymerases in vitro and in bacterial cells in vivo, leading to mutant transcripts by directing incorporation of an incorrect nucleotide during transcription. Such transcriptional mutagenesis (TM) may produce a pool of mutant proteins. In contrast, transcription-couple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saxowsky TT,Meadows KL,Klungland A,Doetsch PW

更新日期：2008-12-02 00:00:00

abstract：:Noninvasive prenatal testing using fetal DNA in maternal plasma is an actively researched area. The current generation of tests using massively parallel sequencing is based on counting plasma DNA sequences originating from different genomic regions. In this study, we explored a different approach that is based on the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu SC,Chan KC,Zheng YW,Jiang P,Liao GJ,Sun H,Akolekar R,Leung TY,Go AT,van Vugt JM,Minekawa R,Oudejans CB,Nicolaides KH,Chiu RW,Lo YM

更新日期：2014-06-10 00:00:00

abstract：:T-helper 17 (Th17) cells have important functions in adaptor immunity and have also been implicated in inflammatory disorders. The bromodomain and extraterminal domain (BET) family proteins regulate gene transcription during lineage-specific differentiation of naïve CD4+ T cells to produce mature T-helper cells. Inhib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung K,Lu G,Sharma R,Vincek A,Zhang R,Plotnikov AN,Zhang F,Zhang Q,Ju Y,Hu Y,Zhao L,Han X,Meslamani J,Xu F,Jaganathan A,Shen T,Zhu H,Rusinova E,Zeng L,Zhou J,Yang J,Peng L,Ohlmeyer M,Walsh MJ,Zhang DY,

更新日期：2017-03-14 00:00:00

abstract：:The activities of cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase [3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acylating), EC 4.1.3.5] and microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase[mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34], two sequential enzymes in the chole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balasubramaniam S,Goldstein JL,Brown MS

更新日期：1977-04-01 00:00:00

abstract：:When Sertoli cells of the seminiferous epithelium in the testis were cultured on Matrigel (a reconstituted basement membrane), laminin, or one of the biologically active laminin-derived peptides (YIGSR, SIKVAV, or RGD), they exhibited dramatic changes in morphology accompanied by changes in protein secretion and gene ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papadopoulos V,Dym M

更新日期：1994-07-19 00:00:00

abstract：:p53 is the most frequently mutated gene in human cancers and mutant p53 has a gain of function (GOF) that promotes tumor progression and therapeutic resistance. One of the major GOF activities of mutant p53 is to suppress 2 other p53 family proteins, p63 and p73. However, the molecular basis is not fully understood. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang J,Sun W,Kong X,Zhang Y,Yang HJ,Ren C,Jiang Y,Chen M,Chen X

更新日期：2019-11-26 00:00:00

abstract：:Flagellar polypeptides of Chlamydomonas reinhardtii were analyzed in two-dimensions by isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate. In addition to flagellar tubulin, over 130 polypeptides were resolved and 100 of these were identified as axonemal components in wild-type organisms...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Piperno G,Huang B,Luck DJ

更新日期：1977-04-01 00:00:00

abstract：:An unsaturated fatty acid-requiring mutant derived from Chinese hamster ovary (CHO) cells has been isolated and characterized. This mutant grows normally when oleate or other unsaturated fatty acids are supplemented in the growth medium. Unlike the wild-type CHO cells, growth stops when medium is deprived of unsaturat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang TY,Vagelos PR

更新日期：1976-01-01 00:00:00

abstract：:Depolarization of isolated nerve terminals (synaptosomes) has been shown to stimulate neurotransmitter release and to increase the phosphorylation state of a number of proteins, including synapsin I, in a Ca2+-dependent manner. Synapsin I, a prominent nerve terminal phosphoprotein, interacts with the cytoplasmic surfa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sihra TS,Wang JK,Gorelick FS,Greengard P

更新日期：1989-10-01 00:00:00

abstract：:Zfp57 is a maternal-zygotic effect gene that maintains genomic imprinting. Here we report that Zfp57 mutants exhibited a variety of cardiac defects including atrial septal defect (ASD), ventricular septal defect (VSD), thin myocardium, and reduced trabeculation. Zfp57 maternal-zygotic mutant embryos displayed more sev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shamis Y,Cullen DE,Liu L,Yang G,Ng SF,Xiao L,Bell FT,Ray C,Takikawa S,Moskowitz IP,Cai CL,Yang X,Li X

更新日期：2015-04-21 00:00:00

abstract：:Polycyclic aromatic hydrocarbons and related species have been suggested to play a key role in the astrochemical evolution of the interstellar medium, but the formation mechanism of even their simplest building block--the aromatic benzene molecule--has remained elusive for decades. Here we demonstrate in crossed molec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones BM,Zhang F,Kaiser RI,Jamal A,Mebel AM,Cordiner MA,Charnley SB

更新日期：2011-01-11 00:00:00

abstract：:Despite the common use of the blooming metaphor, its floral inspiration remains poorly understood. Here we study the physical process of blooming in the asiatic lily Lilium casablanca. Our observations show that the edges of the petals wrinkle as the flower opens, suggesting that differential growth drives the deploym...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang H,Mahadevan L

更新日期：2011-04-05 00:00:00

abstract：:All decisions, whether they are personal, public, or business-related, are based on the decision maker's beliefs and values. Science can and should help decision makers by shaping their beliefs. Unfortunately, science is not easily accessible to decision makers, and scientists often do not understand decision makers' ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von Winterfeldt D

更新日期：2013-08-20 00:00:00

abstract：:Cyclic AMP levels increase abruptly in the secondary palatal shelf (mesenchyme plus a two- to three-cell-layered epithelium) between day 15 and 16 of gestation in the rat embryo. The addition of dibutyryl cyclic AMP to the media in which immature palatal shelves (day 14) are cultured causes a precocious decrease in DN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pratt RM,Martin GR

更新日期：1975-03-01 00:00:00

abstract：:Alphavirus envelope proteins, organized as trimers of E2-E1 heterodimers on the surface of the pathogenic alphavirus, mediate the low pH-triggered fusion of viral and endosomal membranes in human cells. The lack of specific treatment for alphaviral infections motivates our exploration of potential antiviral approaches...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng X,Mukhopadhyay S,Brooks CL 3rd

更新日期：2015-02-17 00:00:00

abstract：:The role of the Ca2+/phospholipid-dependent, diacylglycerol-activated enzyme protein kinase C (PKC) in rabbit eyelid conditioning was examined. PKC was partially purified from the CA1 region of hippocampal slices from naive, pseudoconditioned, and conditioned rabbits 24 hr after the rabbits were well conditioned. Crud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bank B,DeWeer A,Kuzirian AM,Rasmussen H,Alkon DL

更新日期：1988-03-01 00:00:00