Corticoids augment nuclear binding capacity for triiodothyronine in bullfrog tadpole tail fins.

Abstract:

:The effect of aldosterone and corticosterone on T3 binding to the nuclear fraction of the tail fin of bullfrog tadpoles was investigated in vitro. Both corticoids (10(-7) - 10(-5) M) added to the incubation medium increased T3 binding in a dose-dependent manner. The aldosterone-induced increase in T3-binding was blocked by cycloheximide, an inhibitor of protein synthesis, and actinomycin D, an inhibitor of RNA synthesis. Scatchard analysis revealed that 10(-6) M aldosterone and corticosterone increased the maximum binding capacity for T3 by 60 and 41%, respectively, and that the corticoids did not alter the value of the dissociation constant. The significance of the finding is discussed in relation to metamorphosis.

journal_name

Gen Comp Endocrinol

authors

Suzuki MR,Kikuyama S

doi

10.1016/0016-6480(83)90122-3

subject

Has Abstract

pub_date

1983-11-01 00:00:00

pages

272-8

issue

2

eissn

0016-6480

issn

1095-6840

journal_volume

52

pub_type

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