Abstract:
:Vitamin D binding protein (DBP) was isolated from the plasma of the snapping turtle, Chelydra serpentina (mixed sex adult), and compared with the previously characterized dual function binding plasma protein that binds both thyroxine (T4) and vitamin D (tsTDBP) in an emydid turtle, Trachemys scripta. Purification of Chelydra serpentina DBP (csDBP) was accomplished by ion exchange chromatography, preparative polyacrylamide gel electrophoresis, and reverse-phase high-performance liquid chromatography. Estimates of increased purification (ca. 71-fold), recovery (ca. 1.5%), and corresponding plasma concentration (ca. 0.6 mg/ml) are confounded by interference with other proteins. A protein was identified that showed the same high affinity for 25-OH-cholecalciferol (vitamin D3) as the tsTDBP, and the two exhibited similar heat resistances in binding. The csDBP and tsTDBP had similar molecular weights by SDS-PAGE (ca. 58 kDa), showed immunological cross reactivity, and differed by only three residues, representing conservative substitutions, in the 30 NH2-terminal amino acid sequence. A slightly lesser homology (up to 89% similarity based on conservative substitutions) was seen with three mammalian DBPs. However, unlike tsTDBP, the DBP from the snapper did not bind T4. These data support the view that the DBP of emydid turtles secondarily evolved a second T4 binding site, and this is probably independent of the D3 binding domain.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Horowitz M,Licht Pdoi
10.1006/gcen.1996.0182subject
Has Abstractpub_date
1996-12-01 00:00:00pages
364-73issue
3eissn
0016-6480issn
1095-6840pii
S0016-6480(96)90182-3journal_volume
104pub_type
杂志文章abstract::When pigeons were injected with an extract of chicken hypothalamus, the increase in weight of the crop sac showed that prolactin secretion was stimulated. Crop sac weight was no higher after 16 days of injection than it was after 8 days. Pituitary gland content of prolactin and growth hormone was elevated at 8 days, b...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90106-0
更新日期:1984-08-01 00:00:00
abstract::To clarify the physiological roles of the renin-angiotensin-aldosterone system (RAAS) and arginine vasotocin (AVT) on body fluid regulation in amphibians, we measured plasma concentrations of aldosterone (ALDO), angiotensin II (ANG II), and AVT after various osmotic challenges in the marine toad, Bufo marinus (Bufonid...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.10.005
更新日期:2005-01-15 00:00:00
abstract::An investigation into vitamin D-like molecules has been performed on whole extracts of the terrestrial amphipod Orchestia cavimana, using a sensitive nonequilibrium assay employing 1,25-(OH)2 D receptor from calf thymus. Relatively large amounts of these secosteroid-like molecules were observed and they varied in conc...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(91)90070-m
更新日期:1991-10-01 00:00:00
abstract::The gonadotropins (GTHs) follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are the key regulators of reproduction. We determined the competence of heterologous recombinant GTHs at eliciting steroid secretion from carp ovaries at different reproductive stages. We collected carp ovaries at: early, mid and ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2012.04.002
更新日期:2012-08-01 00:00:00
abstract::Dopamine receptors in the pituitary of the African catfish, Clarias gariepinus, were characterized using [3H]spiperone as radioligand. Specific binding of [3H]spiperone to pituitary membranes reached equilibrium within 60 min of incubation. The binding of the radioligand was tissue specific since the amount of binding...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(90)90154-e
更新日期:1990-10-01 00:00:00
abstract::For grass carp (Ctenopharyngodon idella) raised in the Ivory Coast (with water temperatures of 26-31 degrees C), induced spawning is obligatory for fry production. However, ovulation rates following hormonal treatment are often low. We hypothesized that high temperatures are an inhibiting factor for the reproductive a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2003.12.009
更新日期:2004-04-01 00:00:00
abstract::The reproductive biology of the Indian major carp Cirrhinus mrigala is tightly synchronized with the seasonal changes in the environment. While the ovaries show growth from February through June, the fish spawn in July-August to coincide with the monsoon; thereafter the fish pass into the postspawning and resting phas...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(03)00190-4
更新日期:2003-10-01 00:00:00
abstract::The tegu lizard Salvator merianae is a large, widely distributed teiid lizard endemic to South America that exhibits annual cycles of high activity during the spring and summer, and hibernation during winter. This pattern of activity and hibernation is accompanied by profound seasonal changes in physiology and behavio...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.06.006
更新日期:2019-03-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90120-1
更新日期:1987-03-01 00:00:00
abstract::We have found that the inhibition of thyroxine-induced tail fin regression by prolactin in larval tiger salamanders is antagonized by oxytocin. Other workers have shown that prolactin blocks the rise in activity of several hydrolytic enzymes that occurs in regressing tissue during metamorphosis. Here, we examine the e...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90223-6
更新日期:1986-03-01 00:00:00
abstract::In dairy cows, heat stress depresses appetite, leading to decreased food intake, a negative energy balance, and modifies ghrelin levels. Ghrelin is a gut-brain peptide with two major forms: acylated, with an O-n-octanoylated serine in position 3, and nonacylated. To date, the effect of heat stress and estrous cycle on...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.06.006
更新日期:2016-09-01 00:00:00
abstract::Allatostatin-like immunoreactivity was demonstrated in the central nervous system (CNS) neurons of the freshwater pulmonate snails Bulinus globosus (Planorbidae) and Stagnicola elodes (Lymnaeidae). Immunopositive neurons were localized using a monoclonal antibody highly specific to allatostatin I (APSGAQRLYGFGL-amide)...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.6871
更新日期:1997-05-01 00:00:00
abstract::Glucose-induced insulin secretion from pancreatic beta-cells involves metabolism-induced membrane depolarization and voltage-dependent Ca(2+) influx. The electrical events in beta-cell glucose sensing have been studied intensely using mouse islets of Langerhans, but data from other species, including models of type 2 ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.12.017
更新日期:2009-04-01 00:00:00
abstract::We have examined the effect of ambient water temperature on the ability of juvenile rainbow trout to respond to estradiol (E2) injection with vitellogenin (Vg) synthesis. Vg appeared in serum within 24 hr of E2 injection in fish kept at 15 degrees and rose to 70 mg/ml over a 10-day treatment period during which four i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1031
更新日期:1993-02-01 00:00:00
abstract::Isolated brain-retrocerebral neurohemal complex of the silkworm of Bombyx mori was stimulated electrically and the released bombyxin (an insulin-like neuropeptide) was measured using time-resolved fluoroimmunoassay. The amount of bombyxin release depended on the number of stimulus pulses delivered to the axonal tract ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.03.009
更新日期:2004-07-01 00:00:00
abstract::Renal actions of arginine vasotocin (AVT) and atrial natriuretic peptide (ANP) in teleosts are poorly defined. Administration of these hormones changes renal function, largely explained by systemic vascular effects. The present experiments examine the potential direct action of these hormones on the teleost nephron. T...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1046
更新日期:1993-03-01 00:00:00
abstract::The existence of direct progestin actions on teleost sperm to stimulate hypermotility is not widely acknowledged because it has only been demonstrated in members of the family Sciaenidae. In the present study, progestin stimulation of sperm hypermotility was investigated in a non-sciaenid, southern flounder, and the p...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.12.005
更新日期:2011-02-01 00:00:00
abstract::The Atlantic Bluefin Tuna (ABFT, Thunnus thynnus) is one of the most intensely exploited fisheries resources in the world. In spite of the years of studies on ABFT, basic aspects of its reproductive biology remain uncertain. To gain insight regarding the seasonal changes of the reproductive characteristics of the east...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2019.113216
更新日期:2019-10-01 00:00:00
abstract::Fetal development is a critical period of physical development, and factors in the intrauterine environment can cause lasting effects on the growth and development of offspring. There is little research evaluating organizational effects of early androgen exposure of endogenous maternal origins on the prenatal and post...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.10.008
更新日期:2010-04-01 00:00:00
abstract::In songbirds, neurons that regulate learned song behavior undergo extensive seasonal plasticity in their number and size in relation to the bird's reproductive status. Seasonal plasticity of these brain regions is primarily regulated by changes in circulating concentrations of testosterone. Androgen receptors are pres...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.08.001
更新日期:2010-03-01 00:00:00
abstract::Metabolic pathways of estradiol-17 beta and other vertebrate steroid hormones of cultured silkworm pupal ovaries were examined using 14C-labeled steroids. The isolated ovaries showed significant uptake and metabolic activity of the 14C-labeled estradiol-17 beta added to the medium. Analysis of the metabolized compound...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90225-x
更新日期:1986-03-01 00:00:00
abstract::The photoperiodic control of puberty in birds was studied using hybrid red-legged partridges (Alectoris graeca chukar). In the wild partridges first breed at about 10 months of age. Males and females were held from hatch on short (8L:16D) daylengths and then at 3, 4 1/2, 6, 7 1/2, 9, and 12 weeks of age groups were tr...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(88)90290-0
更新日期:1988-07-01 00:00:00
abstract::Insulin-like peptides (ILPs) regulate a multitude of biological processes, including metabolism and immunity to infection, and share similar structural motifs across widely divergent taxa. Insulin/insulin-like growth factor signaling (IIS) pathway elements are similarly conserved. We have shown that IIS regulates repr...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.06.005
更新日期:2011-09-01 00:00:00
abstract::Three experiments were performed to determine the role of juvenile hormone (JH) in worker reproduction in queenless colonies of honey bees. In Experiment 1, egg-laying workers had low hemolymph titers of JH, as did bees engaged in brood care, while foragers had significantly higher titers. Experiment 2 confirmed these...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90055-o
更新日期:1992-09-01 00:00:00
abstract::In addition to the previously recognised thyroid hormone-binding proteins, three plasma components possibly involved in T3 and T4 transport have been identified in chicken plasma using gel filtration at pH 7.4. In mature female, though not male and immature female birds, a significant amount of T3 (11%) is bound to ve...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90276-x
更新日期:1985-02-01 00:00:00
abstract::Variation in food intake affects somatic growth by altering the expression of hormones in the somatotropic endocrine axis including insulin-like growth factor-1 (IGF-1). Here, we examined IGF-1 pathway responses to long- and short-term variation in food availability in copper rockfish (Sebastes caurinus), a nearshore ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2019.04.025
更新日期:2019-09-01 00:00:00
abstract::Arginine vasotocin (AVT) and isotocin (IT) are fish hypothalamic nonapeptides involved in numerous social and reproductive behaviors. Vasotocinergic and isotocinergic fibers project to different brain areas where peptides act as neurotransmitters and/or neuromodulators. In this study, we measured whole brain levels of...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2014.04.039
更新日期:2014-08-01 00:00:00
abstract::Undifferentiated spermatogonia (Aund) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been deve...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.06.019
更新日期:2019-03-01 00:00:00
abstract::A radioimmunoassay (RIA) capable of determining blood ACTH levels in salmonid fishes was developed and validated. The RIA used an antibody raised against mammalian ACTH, iodinated human ACTH as tracer, and human 1-39 ACTH as standard. Incubation of the standard or unknown with antibody for 3 days before addition of as...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90046-8
更新日期:1986-06-01 00:00:00
abstract::Using morpholino antisense oligonucleotide (MO) technology, we blocked leptin A or leptin receptor expression in embryonic zebrafish, and analyzed consequences of leptin A knock-down on fish development. Embryos injected with leptin A or leptin receptor MOs (leptin A or leptin receptor morphants) had smaller bodies an...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2012.07.011
更新日期:2012-09-15 00:00:00