Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts.

abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...

journal_title：Experimental cell research

authors： Zhu S,Wang X,Wang J,Lin J,Cong Y,Qiao G

更新日期：2017-12-01 00:00:00

abstract：:The recruitment of osteoblast progenitors involves their migration and attachment to the sites of bone formation through interactions with matrix proteins. In a time-limited cell attachment assay, coated laminin-1 inhibits the adhesion of most rat calvaria cells but attaches specifically to osteoprogenitors, as quanti...

journal_title：Experimental cell research

authors： Roche P,Rousselle P,Lissitzky JC,Delmas PD,Malaval L

更新日期：1999-08-01 00:00:00

abstract：:Previous morphological and biochemical studies have suggested that actin and actin-containing filaments (microfilaments) exist in the eukaryotic nucleus and that they perform important nuclear functions. However, the concept is not widely accepted. In this study, we demonstrate actin and bundles of actin in the nuclei...

journal_title：Experimental cell research

authors： Parfenov VN,Davis DS,Pochukalina GN,Sample CE,Bugaeva EA,Murti KG

更新日期：1995-04-01 00:00:00

abstract：:In preparation for studies on the growth factor requirements of normal and transformed human fibroblasts, we have developed a serum-free medium that supports vigorous long-term serial subculture of diploid human fibroblasts and allows them to form large-sized colonies with high efficiency (40 to 60%) when plated at cl...

journal_title：Experimental cell research

authors： Ryan PA,Maher VM,McCormick JJ

更新日期：1987-10-01 00:00:00

abstract：:An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...

journal_title：Experimental cell research

authors： Khan S,Collins RJ,Dunn IS

更新日期：1996-02-25 00:00:00

abstract：:The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a m...

journal_title：Experimental cell research

authors： Perego RA,Bianchi C,Brando B,Urbano M,Del Monte U

更新日期：1998-11-25 00:00:00

abstract：:Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...

journal_title：Experimental cell research

authors： Li J,Li H,Tian Y,Yang Y,Chen G,Guo W,Tian W

更新日期：2016-07-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) secrete paracrine factors that could be cytoprotective and serve roles in immunoregulation during tissue injury. Although MSCs express HIV receptors, and co-receptors, and are susceptible to HIV infection, whether HIV-1 may affect biological properties of MSCs needs more study. We evaluat...

journal_title：Experimental cell research

authors： Cheng K,Rai P,Lan X,Plagov A,Malhotra A,Gupta S,Singhal PC

更新日期：2013-08-15 00:00:00

abstract：:A cell culture model stimulating the genetic deficiency of glucocerebrosidase has been developed, utilizing macrophages and conduritol B epoxide (CBE), the specific irreversible inhibitor of the enzyme. Rat peritoneal macrophage glucocerebrosidase was completely inhibited when cells were treated with 10 microM CBE for...

journal_title：Experimental cell research

authors： Das PK,Murray GJ,Gal AE,Barranger JA

更新日期：1987-02-01 00:00:00

abstract：:The growth factor requirement of normal and polyomavirus middle T gene transformed REF52 cells was studied in serum-free medium in an attempt to elucidate the possible linkage between an altered growth factor requirement and one or more altered physiological properties of the transformed cells. For optimal growth, REF...

journal_title：Experimental cell research

authors： Li L,Orlando J,Chen JK

更新日期：1989-07-01 00:00:00

abstract：:Notch signaling involves the proteolytic cleavage of the transmembrane Notch receptor after binding to its transmembrane ligands. Jagged-1 also undergoes proteolytic cleavage by gamma-secretase and releases an intracellular fragment. In this study, we have demonstrated that the Jagged-1 intracellular domain (JICD) inh...

journal_title：Experimental cell research

authors： Kim MY,Jung J,Mo JS,Ann EJ,Ahn JS,Yoon JH,Park HS

更新日期：2011-10-15 00:00:00

abstract：:The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...

journal_title：Experimental cell research

authors： Theiss C,Meller K

更新日期：2002-12-10 00:00:00

abstract：:We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent i...

journal_title：Experimental cell research

authors： Okumoto K,Bogaki A,Tateishi K,Tsukamoto T,Osumi T,Shimozawa N,Suzuki Y,Orii T,Fujiki Y

更新日期：1997-05-25 00:00:00

abstract：:The process of tumor cell invasion of the basement membrane is proposed to consist of three steps: attachment, local proteolysis and migration. 12-(S)-HETE, a 12-lipoxygenase metabolite of arachidonic acid, upregulates surface expression of integrin cytoadhesins and an autocrine motility factor receptor, suggesting th...

journal_title：Experimental cell research

authors： Honn KV,Timár J,Rozhin J,Bazaz R,Sameni M,Ziegler G,Sloane BF

更新日期：1994-09-01 00:00:00

abstract：:Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...

journal_title：Experimental cell research

authors： Zhong ZS,Zhang G,Meng XQ,Zhang YL,Chen DY,Schatten H,Sun QY

更新日期：2005-05-15 00:00:00

abstract：:There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

journal_title：Experimental cell research

authors： D'Silva I,Poirier GG,Heath MC

更新日期：1998-12-15 00:00:00

abstract：:During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...

journal_title：Experimental cell research

authors： Krause S,Fakan S,Weis K,Wahle E

更新日期：1994-09-01 00:00:00

abstract：:The retention of mRNAs near the nuclei that synthesize them may be an important feature of the organization of multinucleated skeletal myotubes. Here, we assess the possible role of two factors in this localization. First, we examine the role of mRNA half-life, by studying the distribution of the mRNA for the transfer...

journal_title：Experimental cell research

authors： Ralston E,McLaren RS,Horowitz JA

更新日期：1997-11-01 00:00:00

abstract：:U73122, a phospholipase C inhibitor, dose dependently blocks the cGMP-induced Ca2+ release in sea urchin eggs and homogenates. U73122 inhibition was prevented by cotreatment with dithiothreitol (DTT), but DTT is ineffective when eggs or homogenates were pretreated with U73122. U73122 action is different from the other...

journal_title：Experimental cell research

authors： Lee SJ,Madden PJ,Shen SS

更新日期：1998-07-10 00:00:00

abstract：:Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in the early stage of pancreatic cancer, contributing to the poor prognosis. Accordingly, in this study, we evaluated the molecular mechanisms underlying invasion and metastasis. Using mass spectrometry, we found that Integrin alp...

journal_title：Experimental cell research

authors： Wu Y,Tan X,Liu P,Yang Y,Huang Y,Liu X,Meng X,Yu B,Wu M,Jin H

更新日期：2019-06-01 00:00:00

abstract：:Subsets of glycoproteins are thought to require lectin-like membrane receptors for efficient export out of the endoplasmic reticulum (ER). To identify new members related to two previously characterized intracellular lectins ERGIC-53/p58 and VIP36, we carried out an extensive database search using the conserved carboh...

journal_title：Experimental cell research

authors： Neve EP,Svensson K,Fuxe J,Pettersson RF

更新日期：2003-08-01 00:00:00

abstract：:Identification of transcription factors expressed by differentiated cells is informative not only of tissue-specific pathways, but to help identify master regulators for cellular reprogramming. If applied, such an approach could generate healthy autologous tissue-specific cells for clinical use where cells from the ho...

journal_title：Experimental cell research

authors： Hustler A,Eardley I,Hinley J,Pearson J,Wezel F,Radvanyi F,Baker SC,Southgate J

更新日期：2018-08-15 00:00:00

abstract：:Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this ...

journal_title：Experimental cell research

authors： Saga H,Kimura K,Hayashi K,Gotow T,Uchiyama Y,Momiyama T,Tadokoro S,Kawashima N,Jimbou A,Sobue K

更新日期：1999-02-25 00:00:00

abstract：:Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...

journal_title：Experimental cell research

authors： Nakahara H,Dennis JE,Bruder SP,Haynesworth SE,Lennon DP,Caplan AI

更新日期：1991-08-01 00:00:00

abstract：:The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline ...

journal_title：Experimental cell research

authors： Tsunoi M,Hakeda Y,Kurihara N,Maeda N,Utsumi N,Kumegawa M

更新日期：1984-07-01 00:00:00

abstract：:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...

journal_title：Experimental cell research

authors： Akatov VS,Lezhnev EI,Vexler AM,Kublik LN

更新日期：1985-10-01 00:00:00

abstract：BACKGROUND:Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to...

journal_title：Experimental cell research

authors： Liu Y,Wang D,Li Y,Yan S,Dang H,Yue H,Ling J,Chen F,Zhao Y,Gou L,Tang P,Huang A,Tang H

更新日期：2019-06-01 00:00:00

abstract：:Procollagen I is a trimer consisting of two proalpha1(I) chains and one proalpha 2(I) chain. In certain cases of mild osteogenesis imperfecta, abnormal proalpha1(I) chains are degraded very soon after synthesis. As a consequence, the cells produce excess proalpha2(I) chains, which cannot form trimers and are not secre...

journal_title：Experimental cell research

authors： Gotkin MG,Ripley CR,Lamande SR,Bateman JF,Bienkowski RS

更新日期：2004-06-10 00:00:00

abstract：:In this report, we elucidate the role of Na(+)-K+ pump in the regulation of polyamine spermidine (Spd) transport in murine leukemia (L 1210) cells in culture. Ouabain, known to bind extracellularly to the alpha-subunit of the Na(+)-K+ pump, inhibits the pump activity. The L 1210 cells were found to possess ouabain bin...

journal_title：Experimental cell research

authors： Khan NA,Quemener V,Moulinoux JP

更新日期：1992-04-01 00:00:00

abstract：:Pitx2 is a bicoid-related homeobox transcription factor implicated in regulating left-right patterning and organogenesis. However, only a limited number of Pitx2 downstream target genes have been identified and characterized. Here we demonstrate that Pitx2 is a transcriptional repressor of DEP domain containing 1B (DE...

journal_title：Experimental cell research

authors： Wu D,Zhu X,Jimenez-Cowell K,Mold AJ,Sollecito CC,Lombana N,Jiao M,Wei Q

更新日期：2015-04-10 00:00:00