Sequences homologous to P elements occur in Drosophila paulistorum.

abstract：:The type 6 secretion system (T6SS) is a nanomachine used by many Gram-negative bacteria, including Vibrio cholerae, to deliver toxic effector proteins into adjacent eukaryotic and bacterial cells. Because the activity of the T6SS is dependent on direct contact between cells, its activity is limited to bacteria growing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toska J,Ho BT,Mekalanos JJ

更新日期：2018-07-31 00:00:00

abstract：:Complete sequences for the intergenic regions of the genome of human respiratory syncytial virus were obtained by dideoxynucleotide sequencing using synthetic oligonucleotides. These experiments established that the 10 respiratory syncytial viral genes are arranged, without additional intervening genes, in the order 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins PL,Dickens LE,Buckler-White A,Olmsted RA,Spriggs MK,Camargo E,Coelingh KV

更新日期：1986-07-01 00:00:00

abstract：:The ciliary epithelium in the eye consists of pigmented epithelial cells that express the α1β1 isoform of Na,K-ATPase and nonpigmented epithelial cells that express mainly the α2β3 isoform. In principle, a Na,K-ATPase inhibitor with selectivity for α2β3 that penetrates the cornea could effectively reduce intraocular p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katz A,Tal DM,Heller D,Habeck M,Ben Zeev E,Rabah B,Bar Kana Y,Marcovich AL,Karlish SJ

更新日期：2015-11-03 00:00:00

abstract：:Protein kinase C (PKC) and Syk protein tyrosine kinase play critical roles in immune cell activation including that through the high-affinity IgE receptor, FcepsilonRI. Mechanisms by which PKC activation leads to the activation of Ras, a family of GTPases essential for immune cell activation, have been elusive. We pre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawakami Y,Kitaura J,Yao L,McHenry RW,Kawakami Y,Newton AC,Kang S,Kato RM,Leitges M,Rawlings DJ,Kawakami T

更新日期：2003-08-05 00:00:00

abstract：:DNA origami enables the precise fabrication of nanoscale geometries. We demonstrate an approach to engineer complex and reversible motion of nanoscale DNA origami machine elements. We first design, fabricate, and characterize the mechanical behavior of flexible DNA origami rotational and linear joints that integrate s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marras AE,Zhou L,Su HJ,Castro CE

更新日期：2015-01-20 00:00:00

abstract：:The complete nucleotide sequence of the gene for the specific glycoprotein (gp55) of the polycythemic strain of Friend spleen focus-forming virus (SFFV) was derived from the cloned SFFV DNA intermediate. The gp55 gene is present within 1.4 kilobases of the 5' side of the 3'long terminal repeat sequence. The open readi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amanuma H,Katori A,Obata M,Sagata N,Ikawa Y

更新日期：1983-07-01 00:00:00

abstract：:Accurate protein folding is essential for proper cellular and organismal function. In the cell, protein folding is carefully regulated; changes in folding homeostasis (proteostasis) can disrupt many cellular processes and have been implicated in various neurodegenerative diseases and other pathologies. For many protei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samelson AJ,Jensen MK,Soto RA,Cate JH,Marqusee S

更新日期：2016-11-22 00:00:00

abstract：:Rat superior cervical sympathetic ganglion was used to begin studying the regulation of molecular components of the synapse. Ganglionic postsynaptic densities (PSDs)exhibited a thin, disc-shaped profile electron microscopically, comparable to that described for brain. Moreover, the presumptive ganglionic PSD protein (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu K,Black IB

更新日期：1987-12-01 00:00:00

abstract：:Human embryonic stem cells have the potential to differentiate into various cell types and, thus, may be useful as a source of cells for transplantation or tissue engineering. We describe here the differentiation steps of human embryonic stem cells into endothelial cells forming vascular-like structures. The human emb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levenberg S,Golub JS,Amit M,Itskovitz-Eldor J,Langer R

更新日期：2002-04-02 00:00:00

abstract：:A high density of galanin binding sites was found by using 125I-labeled galanin, iodinated by chloramine-T, followed by autoradiography in the ventral, but not in the dorsal, hippocampus of the rat. Lesions of the fimbria and of the septum caused disappearance of a major population of these binding sites, suggesting t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fisone G,Wu CF,Consolo S,Nordström O,Brynne N,Bartfai T,Melander T,Hökfelt T

更新日期：1987-10-01 00:00:00

abstract：:Although the apoptotic role of caspases has been largely understood, accumulating evidence in Drosophila suggests that caspases also control other processes than apoptotic cell death. However, how caspases contribute to the development of the mammalian nervous system remains obscure. Here, we provide unique evidence t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohsawa S,Hamada S,Kuida K,Yoshida H,Igaki T,Miura M

更新日期：2010-07-27 00:00:00

abstract：:The chloride permeability of airway and sweat ductal epithelium of cystic fibrosis (CF) patients is decreased. This abnormality could represent an intrinsic characteristic of the epithelial cell or the response to a tonic extrinsic stimulus, in vivo. We cultured airway epithelial cells derived from CF and non-CF indiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stutts MJ,Cotton CU,Yankaskas JR,Cheng E,Knowles MR,Gatzy JT,Boucher RC

更新日期：1985-10-01 00:00:00

abstract：:We have previously shown that nuclear transcripts of the multifunctional enzyme, carbamoyl-phosphate synthetase, aspartate transcarbamylase, dihydroorotase RNA can be released from nuclei of Syrian hamster cells as compact ribonucleoprotein (RNP) particles that sediment at the 200S region in a sucrose gradient. The 20...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spann P,Feinerman M,Sperling J,Sperling R

更新日期：1989-01-01 00:00:00

abstract：:Brief visual experience causes rapid physiological changes in the visual cortex during early postnatal development. A possible mediator of these effects is the immediate early genes whose protein products are involved in the rapid response of neurons to transsynaptic stimulation. Here we report evidence that the level...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosen KM,McCormack MA,Villa-Komaroff L,Mower GD

更新日期：1992-06-15 00:00:00

abstract：:Most studies of cytoskeletal organelles have concentrated on molecular analyses of abundant and biochemically accessible structures. In many of the classical cases, however, the nature of the system chosen has precluded a concurrent genetic analysis. The mitotic spindle of the yeast Saccharomyces cerevisiae is one exa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pillus L,Solomon F

更新日期：1986-04-01 00:00:00

abstract：:Despite their importance for biological activity, slower molecular motions beyond the nanosecond range remain poorly understood. We have assembled an unprecedented set of experimental NMR data, comprising up to 27 residual dipolar couplings per amino acid, to define the nature and amplitude of backbone motion in prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bouvignies G,Bernadó P,Meier S,Cho K,Grzesiek S,Brüschweiler R,Blackledge M

更新日期：2005-09-27 00:00:00

abstract：:Whole-cell and single-channel recording techniques were used to study the action of the anticonvulsant drug MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]- cyclohepten-5,10-imine maleate) on responses to excitatory amino acids in rat neocortical neurons in cell culture. MK-801 caused a progressive, long-lasting bl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huettner JE,Bean BP

更新日期：1988-02-01 00:00:00

abstract：:Constitutive cyclooxygenase (COX-1; prostaglandin-endoperoxide synthase, EC 1.14.99.1) is present in cells under physiological conditions, whereas COX-2 is induced by some cytokines, mitogens, and endotoxin presumably in pathological conditions, such as inflammation. Therefore, we have assessed the relative inhibitory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell JA,Akarasereenont P,Thiemermann C,Flower RJ,Vane JR

更新日期：1993-12-15 00:00:00

abstract：:Based on structural information derived from the F NMR data of labeled rhodopsins, rhodopsin crystal structure, and excited-state properties of model polyenes, we propose a molecular mechanism that accounts specifically for the causes of the well-known enhanced photoreactivity of rhodopsin (increased rates and quantum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu RS,Colmenares LU

更新日期：2003-12-09 00:00:00

abstract：:Natural abundance 13C spin-lattice (T1) relaxation time measurements are reported for unilamellar vesicles of 1,2-dipalmitoylphosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), in the liquid crystalline phase, at magnetic field strengths of 1.40, 1.87, 2.35, 4.23, 7.05, 8.45, and 11.7 tesla (resonance f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown MF,Ribeiro AA,Williams GD

更新日期：1983-07-01 00:00:00

abstract：:Protective immunity against human schistosome infection develops slowly, for reasons that are not yet fully understood. For many decades, researchers have attempted to infer properties of the immune response from epidemiological studies, with mathematical models frequently being used to bridge the gap between immunolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell KM,Mutapi F,Savill NJ,Woolhouse ME

更新日期：2012-08-14 00:00:00

abstract：:The exopolysaccharide alginate is a major virulence factor in the pathogenicity of Pseudomonas aeruginosa infecting the lungs of cystic fibrosis (CF) patients. Alginate synthesis by P. aeruginosa is believed to occur in response to environmental signals present in the CF lung. Transcription of a critical alginate bios...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roychoudhury S,Sakai K,Chakrabarty AM

更新日期：1992-04-01 00:00:00

abstract：:Lactobacillus acidophilus NCFM is a probiotic bacterium that has been produced commercially since 1972. The complete genome is 1,993,564 nt and devoid of plasmids. The average GC content is 34.71% with 1,864 predicted ORFs, of which 72.5% were functionally classified. Nine phage-related integrases were predicted, but ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Altermann E,Russell WM,Azcarate-Peril MA,Barrangou R,Buck BL,McAuliffe O,Souther N,Dobson A,Duong T,Callanan M,Lick S,Hamrick A,Cano R,Klaenhammer TR

更新日期：2005-03-15 00:00:00

abstract：:5-enol-Pyruvylshikimate-3-phosphate synthase (EPSP synthase; phosphoenolpyruvate:3-phosphoshikimate 1-carboxyvinyltransferase, EC 2.5.1.19) is an enzyme on the pathway toward the synthesis of aromatic amino acids in plants, fungi, and bacteria and is the target of the broad-spectrum herbicide glyphosate. The three-dim...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stallings WC,Abdel-Meguid SS,Lim LW,Shieh HS,Dayringer HE,Leimgruber NK,Stegeman RA,Anderson KS,Sikorski JA,Padgette SR,Kishore GM

更新日期：1991-06-01 00:00:00

abstract：:Fluoride activation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] is significantly enhanced (2 to 5 times) by a protein factor isolated from rat brain. The fluoride-dependent adenylate cyclase stimulator (FCS) is nondialyzable, trypsin-labile, and stable at 90 degrees C for 10 min. FCS stimula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rasenick MM,Bitensky MW

更新日期：1980-08-01 00:00:00

abstract：:As a dynamic property of folded proteins, protein compressibility provides important information about the forces that govern structural stability. We relate intrinsic compressibility to stability by using molecular dynamics to identify a molecular basis for the variation in compressibility among globular proteins. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dadarlat VM,Post CB

更新日期：2003-12-09 00:00:00

abstract：:The visual images in the eyes contain much more information than the brain can process. An important selection mechanism is feature-based attention (FBA). FBA is best described by attention filters that specify precisely the extent to which items containing attended features are selectively processed and the extent to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun P,Chubb C,Wright CE,Sperling G

更新日期：2016-10-25 00:00:00

abstract：:Nonribosomal peptide synthetases (NRPSs) usually catalyze the biosynthesis of peptide natural products by sequential selection, activation, and condensation of amino acid precursors. It was reported that some fatty acids, α-ketoacids, and α-hydroxyacids originating from amino acid metabolism as well as polyketide-deri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng C,Pu JY,Song LQ,Jian XH,Tang MC,Tang GL

更新日期：2012-05-29 00:00:00

abstract：:New important applications of copper metal, e.g., in the areas of hydrogen production, fuel cell operation, and spent nuclear fuel disposal, require accurate knowledge of the physical and chemical properties of stable and metastable copper compounds. Among the copper(I) compounds with oxygen and hydrogen, cuprous oxid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Korzhavyi PA,Soroka IL,Isaev EI,Lilja C,Johansson B

更新日期：2012-01-17 00:00:00

abstract：:Transcription of a right-handed double-helical DNA requires a relative rotation of the RNA polymerase and its nascent RNA around the DNA. We describe conditions under which the resistance to the rotational motion of the transcription ensemble around the DNA can be large. In such cases, the advancing polymerase generat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu LF,Wang JC

更新日期：1987-10-01 00:00:00